Localization of cyclin A at the sites of cellular DNA replication.
Exp Cell Res
; 206(1): 43-8, 1993 May.
Article
em En
| MEDLINE
| ID: mdl-8097727
ABSTRACT
Cyclin A is a nuclear protein which is part of a kinase complex with either p34cdc2 or p33cdk2. Cyclin A is required in higher eukaryotic cells at the G1/S and the G2/M transitions. To examine the relationship between cyclin A and DNA replication, we simultaneously labeled exponentially growing HeLa cells for the distribution of cyclin A and proliferating cell nuclear antigen (PCNA). We have now demonstrated, by means of immunoelectron microscopy, that cyclin A is located at the sites of DNA replication visualized by both BrdU and PCNA labeling. Thus cyclin A may play a significant role in the phosphorylation of proteins at or near the sites of DNA replication.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ciclinas
/
Replicação do DNA
Limite:
Humans
Idioma:
En
Revista:
Exp Cell Res
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
França