Molecular basis for developmental changes of GM-CSF gene inducibility in embryonal carcinoma cells.
Biochem Biophys Res Commun
; 198(2): 473-9, 1994 Jan 28.
Article
em En
| MEDLINE
| ID: mdl-8297357
ABSTRACT
In a previous report, we reported that the induction of GM-CSF gene in differentiated P19 cells results from the maturation of the transcriptional machinery. Here, we identified a cis-DNA element which confers the activation of GM-CSF gene in response to PMA/A23187 stimulation in differentiated state. Analysis of the 5'-flanking region between -113 and -60 revealed two elements responsible for promotion and one for inhibition, and the overall effects led to the activation of GM-CSF gene mainly through the sequence between -95 and -73. Using the oligonucleotide between -94 and -73 as a probe in gel retardation assays, we identified a DNA-binding protein, NF-GM-P19, the binding activity of which was induced after differentiation in response to PMA/A23187 stimulation. These results indicate that the induction of GM-CSF gene after differentiation results from the maturation of the transcriptional machinery which recognizes the sequence between -95 and -73.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA de Neoplasias
/
Regulação Neoplásica da Expressão Gênica
/
Sequências Reguladoras de Ácido Nucleico
/
Fator Estimulador de Colônias de Granulócitos e Macrófagos
/
Carcinoma Embrionário
/
Proteínas de Ligação a DNA
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Japão