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Two human homologs of Rad23 are functionally interchangeable in complex formation and stimulation of XPC repair activity.
Sugasawa, K; Ng, J M; Masutani, C; Maekawa, T; Uchida, A; van der Spek, P J; Eker, A P; Rademakers, S; Visser, C; Aboussekhra, A; Wood, R D; Hanaoka, F; Bootsma, D; Hoeijmakers, J H.
Afiliação
  • Sugasawa K; Department of Cell Biology and Genetics, Medical Genetic Centre, Erasmus University, Rotterdam, The Netherlands. sugasawa@postman.riken.go.jp
Mol Cell Biol ; 17(12): 6924-31, 1997 Dec.
Article em En | MEDLINE | ID: mdl-9372924
ABSTRACT
XPC-hHR23B protein complex is specifically involved in nucleotide excision repair (NER) of DNA lesions on transcriptionally inactive sequences as well as the nontranscribed strand of active genes. Here we demonstrate that not only highly purified recombinant hHR23B (rhHR23B) but also a second human homolog of the Saccharomyces cerevisiae Rad23 repair protein, hHR23A, stimulates the in vitro repair activity of recombinant human XPC (rhXPC), revealing functional redundancy between these human Rad23 homologs. Coprecipitation experiments with His-tagged rhHR23 as well as sedimentation velocity analysis showed that both rhHR23 proteins in vitro reconstitute a physical complex with rhXPC. Both complexes were more active than free rhXPC, indicating that complex assembly is required for the stimulation. rhHR23B was shown to stimulate an early stage of NER at or prior to incision. Furthermore, both rhHR23 proteins function in a defined NER system reconstituted with purified proteins, indicating direct involvement of hHR23 proteins in the DNA repair reaction via interaction with XPC.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Ligação a DNA / Reparo do DNA Limite: Humans Idioma: En Revista: Mol Cell Biol Ano de publicação: 1997 Tipo de documento: Article País de afiliação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Ligação a DNA / Reparo do DNA Limite: Humans Idioma: En Revista: Mol Cell Biol Ano de publicação: 1997 Tipo de documento: Article País de afiliação: Holanda
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