A new member of the Rho family, Rnd1, promotes disassembly of actin filament structures and loss of cell adhesion.
J Cell Biol
; 141(1): 187-97, 1998 Apr 06.
Article
em En
| MEDLINE
| ID: mdl-9531558
ABSTRACT
Members of the Rho GTPase family regulate the organization of the actin cytoskeleton in response to extracellular growth factors. We have identified three proteins that form a distinct branch of the Rho family Rnd1, expressed mostly in brain and liver; Rnd2, highly expressed in testis; and Rnd3/RhoE, showing a ubiquitous low expression. At the subcellular level, Rnd1 is concentrated at adherens junctions both in confluent fibroblasts and in epithelial cells. Rnd1 has a low affinity for GDP and spontaneously exchanges nucleotide rapidly in a physiological buffer. Furthermore, Rnd1 lacks intrinsic GTPase activity suggesting that in vivo, it might be constitutively in a GTP-bound form. Expression of Rnd1 or Rnd3/RhoE in fibroblasts inhibits the formation of actin stress fibers, membrane ruffles, and integrin-based focal adhesions and induces loss of cell-substrate adhesion leading to cell rounding (hence Rnd for "round"). We suggest that these proteins control rearrangements of the actin cytoskeleton and changes in cell adhesion.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
/
Adesão Celular
/
Actinas
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Proteínas de Ligação ao GTP
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Proteínas rho de Ligação ao GTP
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GTP Fosfo-Hidrolases
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Humans
/
Male
Idioma:
En
Revista:
J Cell Biol
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Reino Unido