Deferoxamine arrests in vitro the proliferation of porcine hepatocyte in G1 phase of the cell cycle.
Liver
; 18(1): 60-6, 1998 Feb.
Article
em En
| MEDLINE
| ID: mdl-9548269
ABSTRACT
Iron is required for cell proliferation of all living species. Moreover, iron excess may be involved in the development of hepatocellular carcinoma. In this study we analyzed the effects of deferoxamine, an iron chelator, on normal porcine hepatocyte proliferation. We confirmed that hepatocytes isolated from young pigs proliferate in the presence of insulin and fetal calf serum as shown by [3H] methyl-thymidine incorporation, presence of mitotic figures and increase in cell number. This was paralleled by nuclear expression of p34cdc2 and its associated histone H1 kinase activity. In the presence of deferoxamine, [3H] methyl-thymidine incorporation, expression of nuclear proteins (p34cdc2 and PCNA) and H1 kinase activity were drastically reduced. In addition, in contrast with control cultures, cells in S-phase were not detected by flow cytometry. These data suggest that iron chelation by deferoxamine can arrest the progression of porcine hepatocytes in the G1 phase of the cell cycle.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Quelantes de Ferro
/
Fase G1
/
Desferroxamina
/
Replicação do DNA
/
Fígado
Limite:
Animals
Idioma:
En
Revista:
Liver
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
França