Effects of glycosylation of the residue at position 14 in ovine angiotensinogen on the human renin reaction.
Biosci Biotechnol Biochem
; 62(8): 1612-4, 1998 Aug.
Article
em En
| MEDLINE
| ID: mdl-9757569
ABSTRACT
A mutant angiotensinogen, S14N, in which Ser14 of ovine angiotensinogen was replaced by Asn to form a N-glycosylation site, was produced in CHO cells. The molecular weight was about 3,000 larger than that of wild-type ovine angiotensinogen, indicating that S14N angiotensinogen was glycosylated at Asn14. In the reaction with human renin, the km of mutant angiotensinogen was 3 times increased, but the Vmax was not affected by the mutation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Angiotensinogênio
/
Renina
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biosci Biotechnol Biochem
Assunto da revista:
BIOQUIMICA
/
BIOTECNOLOGIA
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Japão