[Pharmacologic analysis of the role of catecholamines in the mechanism of alcoholic affection of the heart]. / Farmakologicheskii analiz roli katekholaminov v mekhanizme alkogol'nogo porazheniia serdtsa.

The authors studied the effect of 6-oxidopamine and rausedyl on the manifestation of cardiac disorders in rats given 3-5 g/kg ethanol by way of the stomach at 12-hour intervals for 5.5 days. 6-oxidopamine was injected intraperitoneally in a dose of 50 mg/kg 24 hours before the beginning of alcoholization and one hour after the first administration of ethanol. Rausedyl was given in a dose of 0.5 mg/kg by the intragastric route once a day during alcoholization. It was established in perfusion of an isolated rat heart that ethanol induces decrease of cardiac rhythm and rate of relaxation of the heart and coronary duct, increase of systolic and diastolic pressure in the left ventricle, and escape of creatine phosphokinase from the heart. 6-oxidopamine fails to influence while rausedyl weakens the effect of ethanol on heart contractility. Both compounds reduce the escape of creatine phosphokinase from the heart approximately by 40%. It is concluded that the destructive effect of ethanol on the cardiomyocytes is mediated partly by catecholamines. The authors suggest that the cardiotoxic effect of catecholamines in alcoholic intoxication is realized not fully because ethanol weakens their negative action on the heart.

[Determination of the leading factors in the pathogenesis of an alcoholic lesion of the heart]. / Opredelenie vedushchikh faktorov v patogeneze alkogol'nogo porazheniia serdtsa.

Male rats were given per os 25% ethanol solution twice a day at 9.00 and 21.00 for 5.5 consecutive days. Every single dose was 2 to 5 g/kg 2 and 12 hours after 8th gavage ethanol, acetaldehyde and acetone concentrations were measured in blood, 2-8 hours after the last (11th) gavage isolated hearts were perfused by Krebs-Henseleit solution. Applying of Spearman rank correlation method demonstrated negative correlation between mean acetaldehyde concentration and maximal systolic pressure, tension-time index of left ventricle and velocity of contraction and relaxation. Negative correlation has been shown between maximal ethanol concentration (MEC) and rate heart whereas positive correlation has been noticed between MEC and leakage of creatine phosphokinase.

[Comparative characteristics of voluntary and semivoluntary methods of the alcoholization of rats]. / Sravnitel'naia kharakteristika dobrovol'nykh i poludobrovol'nykh metodov alkogolizatsii krys.

Water and ethanol consumption, blood and urine ethanol concentrations were measured in male rats aged 1.5 to 8 months. The animals had ethanol solutions (5-25%) and water as alternate fluid (two-bottle choice) or a 10% ethanol solution as a sole water source. In both cases, the rats did not exceed 7 g/kg of ethanol consumption per day. From 10 a.m. to 16 p.m. the blood ethanol concentration was no more than 0.1 g/l. Ethanol excretion with urine did not go beyond 0.1% of the daily dose. Ethanol consumption was increased by 1-2 g/kg a day if saccharin (0.125%) and sodium chloride (1%) were added to ethanol solution. In this case the withdrawal signs developed after ethanol consumption cessation.