Subject(s)
Cattle/genetics , Polymorphism, Restriction Fragment Length , Repetitive Sequences, Nucleic Acid , Animals , Base Sequence , Chromosome Mapping , DNA Primers/genetics , Genetic Markers , Genomic Library , Male , Molecular Sequence Data , Oligodeoxyribonucleotides/genetics , Polymerase Chain ReactionABSTRACT
Altogether 424 Norwegian AI bulls, progeny tested for clinical mastitis, ketosis and fertility (recorded as nonreturn percentage), were typed by Edinburgh and Oslo allo-antisera to bovine lymphocyte antigens (BoLA-A) over a 7-year period. Significant effects of BoLA-A on disease were revealed. A2 was associated with relative resistance to mastitis, a positive influence on fertility, and a possible relative resistance to ketosis, while A13 was associated with relative resistance to ketosis. The previously reported associations of A11 and w16 with relative susceptibility to mastitis were not confirmed in the present material.
Subject(s)
Cattle Diseases/immunology , Fertility/genetics , Histocompatibility Antigens/analysis , Ketosis/veterinary , Mastitis, Bovine/immunology , Animals , Cattle , Cattle Diseases/genetics , Female , Fertility/immunology , Ketosis/genetics , Ketosis/immunology , Male , Mastitis, Bovine/geneticsABSTRACT
Bovine lymphocyte antigens of class I were defined for 434 AI bulls of Norwegian Cattle, which is a dual purpose breed. The antigens w16, A2, and A8 occurred at highest frequencies. A gene substitution model was used to compare bovine lymphocyte antigens with total breeding value and breeding values for milk yield, milk fat percentage, milk protein percentage, and meat production. Several bovine lymphocyte antigens were significantly associated with the traits in question: low fat percentage was associated with A8, A10(w50), and A11; high milk protein percentage with A13; and low meat production with the rare antigen w1. Associations of the bovine lymphocyte antigens and milk yield were not significant.
Subject(s)
Cattle/genetics , Histocompatibility Antigens Class I/genetics , Lactation/genetics , Meat , Alleles , Animals , Female , Gene Frequency , Genetic Linkage , Genetic Markers , Male , Models, Genetic , NorwayABSTRACT
Serum defined BoLA-A antigens, together with BoLA-DQB RFLP patterns, were determined in 87 almost unrelated Norwegian cattle. Statistical analysis revealed strong linkage disequilibria between these loci at the population level. A total of 13 haplotypes were found to be present at frequencies significantly greater than those predicted on the basis of their component gene frequencies. Among these, the subgroups 1A and 1B of the DQ1 haplotype were found to be closely associated with the class I antigens A11 and w16, respectively. The association between A11 and DQ1A is of particular interest, as two independent studies, one employing class I serology, and the other RFLP analysis of the class II locus DQ, have previously indicated that A11 and DQ1A confer relative susceptibility to mastitis.
Subject(s)
Cattle/genetics , Genes, MHC Class II , Genes, MHC Class I , Linkage Disequilibrium , Alleles , Animals , Cattle/immunology , Gene Frequency , Polymorphism, Restriction Fragment LengthABSTRACT
A total of 102 cows was tested for class I antigens of the bovine major histocompatibility complex. Half of the animals (51) had completed at least four lactations without any veterinary treatment for mastitis. The distribution of BoLA-A antigens among these relative mastitis-resistant cows was compared to that in the other half of the material (51), which comprised animals with at least one recorded treatment for mastitis. There were no statistically significant differences in BoLA-A antigen frequency between cows with mastitis and cows without mastitis. The two most common antigens were A2 and w16. The frequency of these two antigens deviated from earlier estimates within the Norwegian cattle (NRF) population, the difference for w16 being statistically significant.