ABSTRACT
The bruchid, Callosobruchus maculatus (F.) causes major losses during the storage of cowpea seeds [Vigna unguiculata (L.)Walp.] in Sri Lanka. Essential oil isolated from Plectranthus zeylanicus plant was tested for potential insecticidal activity against C. maculatus. The gas chromatography studies of the essential oil of P. zeylanicus showed that ρ-cymene (3.5%), ß-caryophyllene (0.2%), geranyl acetate (9.3%) and geraniol (7.2%) were the major constituents. The adults of C. maculatus were susceptible to both fumigant and contact toxicity of P. zeylanicus plant oil. LC(50) values of 0.927 and 0.010 g L(-1) were obtained for fumigant toxicity and contact toxicity assays, respectively. Oviposition and F(1) adult emergence were significantly inhibited by P. zeylanicus plant oil at a concentration higher than 0.001 g L(-1) in both fumigant and contact toxicities. The analysis of olfactometer and choice camber bioassays revealed the repellent effects of the oil of P. zeylanicus plant.
Subject(s)
Coleoptera/drug effects , Insecticides/pharmacology , Oils, Volatile/chemistry , Oils, Volatile/pharmacology , Plectranthus/chemistry , Acetates/analysis , Acyclic Monoterpenes , Animals , Chromatography, Gas , Cymenes , Dose-Response Relationship, Drug , Drug Evaluation, Preclinical/methods , Edible Grain , Female , Insect Repellents/pharmacology , Monoterpenes/analysis , Mortality , Oils, Volatile/analysis , Oviposition/drug effects , Polycyclic Sesquiterpenes , Sesquiterpenes/analysis , Sri Lanka , Terpenes/analysisABSTRACT
The carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1) is downregulated in colonic and intestinal hyperplastic lesions as well as in other cancers, where it functions as a tumor suppressor. To investigate the functions of CEACAM1 in the normal intestine and in intestinal tumors, we generated a compound knockout mouse model and examined both Ceacam1(-/-) and Apc(1638N/+):Ceacam1(-/-) mice. Ceacam1(-/-) intestinal cells exhibited a significant decrease in apoptosis, with no change in proliferation or migration, however. Compound Apc(1638N/+):Ceacam1(-/-) mice demonstrated an increase in intestinal tumor multiplicity and tumor progression. Increases in intussusceptions and desmoid lesions were also observed. We have shown that CEACAM1-L associates with beta-catenin by co-immunoprecipitation and colocalization in CEACAM1-L-transfected CT26 and CT51 mouse colon carcinoma cells. Ceacam1(-/-) enterocytes displayed decreased glycogen synthase kinase 3-beta activity with corresponding nuclear localization of beta-catenin. Increased T-cell factor/Lef transcriptional activity was observed in CEACAM1-null CT51 colonic cells and in Caco2 colon cancer cells in which CEACAM1 was downregulated. A significant increased expression in c-Myc and cyclin D1 targets of the Wnt signaling pathway was also revealed in the Ceacam1(-/-) intestine. CEACAM1 therefore actively participates in Wnt signaling in intestinal cells and its downregulation in intestinal tissue contributes to malignancy by augmenting tumor multiplicity and progression.
Subject(s)
Carcinoembryonic Antigen/physiology , Intestinal Neoplasms/pathology , Signal Transduction , Wnt Proteins/metabolism , Animals , Base Sequence , Carcinoembryonic Antigen/genetics , Cell Line, Tumor , DNA Primers , Disease Progression , Mice , Mice, Inbred C57BL , Mice, Knockout , Reverse Transcriptase Polymerase Chain ReactionABSTRACT
Gastrocystoplasty is a form of surgical bladder augmentation or neobladder used to restore bladder capacity and compliance in children and in patients with neurogenic bladder. Other forms of bladder augmentation include ileocystoplasty and colocystoplasty. Reported complications of gastrocystoplasty include post-operative bleeding, haematuria, stricture, metabolic alkalosis and rupture of the gastric segment. There are reports of adenocarcinomas arising in the setting of ileocystoplasty and colocystoplasty. However, the first case of adenocarcinoma arising in the setting of a gastrocystoplasty is reported.
Subject(s)
Adenocarcinoma/etiology , Stomach/transplantation , Urinary Bladder Neoplasms/etiology , Urinary Bladder, Neurogenic/surgery , Adenocarcinoma/pathology , Humans , Male , Middle Aged , Urinary Bladder Neoplasms/pathologyABSTRACT
Poorly differentiated malignancies affecting the anal canal are uncommon but pose diagnostic difficulties because of the wide range of normal cell types that may occur within a limited anatomical region. The range of lesions that may present as poorly differentiated tumours includes squamous cell carcinoma, adenocarcinoma, small and large cell neuroendocrine carcinoma, neuroendocrine carcinoma expressing epithelial cytokeratins and other patterns of mixed differentiation, undifferentiated carcinoma, malignant melanoma, lymphoma and secondary tumours. This review discusses the differential diagnosis of these neoplasms with the aid of short illustrative case studies.