[The social hygienic risk factors to be applied in acute endometritis prognostication].

The article presents the results of investigation of prevalence and prognostic significance of socio-hygienic risk factors of acute endometritis in 187 patients with this disease and 150 patients with non-complicated postnatal period. It is established that development of acute endometritis is associated more with quality of life indices, early (up to 14 years of age) onset of sexual activity, presence in anamnesis of medical and spontaneous abortion. These risk factors should be considered in the process of prognosticating development of acute endometritis.

[Laboratory diagnostics of ovarian cancer among the elderly in the parameters of immunoglobulins and phagocytic activity of blood.]

Due to the increase in the proportion of elderly women, the prevalence of ovarian cancer is increasing, which requires the search for new methods of its diagnosis. The aim of the work is to analyze the content and informativeness of immunoglobulins and phagocytic activity of peripheral blood to improve the diagnosis of ovarian cancer among elderly women. In 78 patients with ovarian cancer aged 65-70 years immunological and spectrophotometric methods studied phagocytic activity and the level of peripheral blood immunoglobulins. 42 women of the same age with no ovarian cancer served as control. The primary importance of circulating immune complexes, phagocyte migration inhibition index and neutrophil activity index for laboratory diagnosis of ovarian cancer among the elderly was established. The use of highly informative parameters of systemic immunity improves the diagnosis of ovarian cancer in the elderly.

[The development of diagnostic of acute endometritis on the basis of modeling and cluster analysis of parameters of local immunity.]

The acute endometritis provoking expressed disorders of local immunity is considered as one of the prevalent complications of postnatal period and abortions. The purpose of study is to develop diagnostic of acute endometritis on the basis of applied correlation modeling and cluster analysis techniques diagnostically priority-driven immunological indices of vaginal cervical mucus. The sample included main group (154 patients with acute endometritis) and control group (103 patients with uncomplicated post-natal period). Both groups were subjected to enzyme-linked immunosorbent assay to analyze content of immunoglobulins, components of complement and lysozyme in vaginal cervical mucus. The sampling of diagnostically significant indices was implemented using correlation and cluster analysis techniques. It is established that chief diagnostic indices of local immunity in case of acute endometritis are IG M, sIg A, С4 components of complement and lysozyme. The established diagnostically significant parameters of local immunity provide development of detection of acute endometritis according less number of indices.

[Immunoassay and discriminant analysis to improve the diagnosis of ovarian cancer in the elderly.]

Ovarian cancer is a common pathology in the structure of tumors among elderly women. The aim of the work is to identify the leading indicators of interleukin profile to improve the diagnosis of ovarian cancer in the elderly. Enzyme immunoassay studied serum levels of interleukins in 78 patients with ovarian cancer stage IIB-IIIB according to the classification of FIGO at the age of 65-70 years and 42 women of similar age without ovarian cancer at the time of the study and in history. The differentiation of the compared groups is carried out by means of discriminant method. The leading diagnostic interleukins for ovarian cancer in the elderly are IL-1α, IL-1ß, IL-8 and TNF-α. The constructed discriminant models allow to differentiate patients with ovarian cancer and control women with an accuracy of 93.2-93.8%. The use of enzyme immunoassay and discriminant analysis improve the diagnosis of ovarian cancer in the elderly.

[Diagnosis of acute endometritis on hematologic indicators, and given discriminant models.]

Diagnosis of acute endometritis, despite advances in modern medicine due to the prevalence of erased forms remains difficult. The purpose of the study is to improve the diagnosis of acute endometritis by informative indicators of peripheral blood and discriminant models. In 154 patients with acute postpartum endometritis and 103 patients with uncomplicated post-Natal period studied, the indicators of General blood analysis with the assessment of informativeness, with the construction of the correlation and discriminant models. On the basis of mathematical models has high diagnostic significance of band neutrophils, erythrocyte sedimentation rate. These blood parameters have the largest number of reliable correlations with the other formed elements. The development of acute endometritis is accompanied by an increase in the number of representative correlations between blood parameters. The discriminant model accurately diagnose acute endometritis in to 86.7-87.4%, other cases are informative indicators of the General analysis of blood.

[Reflux nephropathy in children: early diagnosis and monitoring].

Vesicoureteral reflux (VUR) is the most common type of obstructive uropathy in children. Reflux nephropathy (RN) is one of the most common complications of VUR that inevitably leads to chronic kidney disease. Patients with end-stage kidney disease require costly treatment, and the only way to cure them is kidney transplantation. A timely institution of renoprotective therapy is a key factor helping to preserve the function of the native kidneys. Hence, it is necessary to devise new highly sensitive and minimally invasive methods for early diagnosis of RN. The purpose of this article is to review the molecular mechanisms of initiation and progression of kidney fibrosis and the opportunities of instrumental and non-instrumental methods for its diagnosis. Special attention is paid to highly specific and highly sensitive non-invasive methods for the detection of minimal changes in the renal parenchyma. The authors discuss the promising biomarkers for the diagnosis and prediction of RN.

[The rationalization of laboratory diagnostic of acute salpingooophoritis according informative parameters of humoral immunity.]

The acute salpingoophoritis is a wide-spread pathology among gynecological diseases brining on significant disorders in the humoral immune system. The study was carried out to rationalize laboratory diagnostic of acute salpingoophoritis according to informative parameters of humoral immunity. The analysis of indices of humoral immunity was applied to 65 patients with acute salpingoophoritis and 32 healthy women using radial immunodiffusion technique. The significant increasing of both of circulating immune complexes in blood plasma and related correlation relationship of IgA, IgG is established. The high informativeness of the given indices is established. The leading diagnostic significance in laboratory diagnostic is related to IgA, IgG and circulating immune complexes.

Rotation thromboelastometry analysis of clot formation and fibrinolysis in severe thrombocytopenia: effect of fibrinogen, activated prothrombin complex concentrate, and thrombin- activatable fibrinolysis inhibitor.

INTRODUCTION: Bleeding symptoms in severe thrombocytopenia range from mild to severe. The aim of this in vitro study was to improve blood clotting and protect against fibrinolysis in reconstituted severe thrombocytopenia blood. METHODS: Thrombocytopenia [(16 ± 4) × 10(6) /mL] was created by high-speed centrifugation of normal blood with subsequent mixing plasma with packed cells. The blood samples were subjected to clotting by CaCl2 and tissue factor and to fibrinolysis by the addition of tissue plasminogen activator. Blood was spiked with fibrinogen, activated prothrombin complex concentrate (FEIBA), thrombin activatable fibrinolysis inhibitor (TAFI), or their combinations. To mimic the situation that may occur in patients subjected to massive transfusion of plasma substitutes, blood was diluted by 40% of TRIS/saline buffer. Clotting time (CT), α-Angle, maximum clot firmness (MCF), and lysis onset time (LOT) were evaluated using rotation thromboelastometry. RESULTS: Spiking thrombocytopenia blood with FEIBA led to reduction of CT. Fibrinogen and FEIBA enhanced α-Angle and MCF both in the absence and in the presence of tPA. LOT values were prolonged by TAFI and to less extent by FEIBA. Dilution of thrombocytopenia blood was followed by reduction of α-Angle and MCF compared to nondiluted blood which partly reversed by either fibrinogen or FEIBA being higher using fibrinogen and FEIBA together. Clot strength was enhanced, and fibrinolysis was inhibited by TAFI. CONCLUSION: The results of this study suggest that combined spiking of blood with fibrinogen and FEIBA may be enough to correct the clot formation disorder in severe thrombocytopenia, whereas in thrombocytopenia and blood dilution, additive inhibition of fibrinolysis may be needed.

[Influence of laser radiation of the whole blood in vitro on adhesion and aggregation of blood platelets].

The authors revealed dependence of reaction blood plates to photoeffect on the dose and rate of blood movement at laser radiation of donor blood in vitro. The red light decreases adhesion and aggregation of blood plates both at high and low rate of shift. Infrared laser radiation is effective only at high rate of shift leading to increase of adhesion and decrease of aggregation of blood plates. Blue laser is effective in small doses only and at low rate of sift it leads to decrease of adhesion and at high rate it provokes increase of adhesion. Blue laser do not have a significant influence on aggregation of blood plates. These results make possible to suppose ambiguity of biological response of venous and arterial blood to radiation.