ABSTRACT
Medullary thyroid cancer (MTC) accounts for around 5-10% of all thyroid cancers. Though usually sporadic, 1 in 4 cases are of genetic origin, with germinal mutations in the RET proto-oncogene in familial forms and somatic mutations both in RET and in the RAS family genes in sporadic ones.This study aimed to characterize a rare H-RAS sequence variant -M72I- in a patient with sporadic MTC, focusing on its functional significance.Mutation analysis was performed for the RET, N-RAS, K-RAS and H-RAS genes by direct sequencing. Western blot analysis was done on 4 thyroid tissues from 1 patient carrying the M72I mutation in H-RAS, 1 with the Q61R mutation in H-RAS, 1 with no RET, H-RAS, K-RAS or N-RAS gene mutations, and 1 normal thyroid, using different antibodies against Erk1/2, phospho-Erk1/2 (Thr202/Tyr204), Akt and phospho-Akt (Ser473). Large-scale molecular dynamics simulations were completed for H-RAS wt and H-RAS M72I.Western blot analysis demonstrated that both MAPK and PI3K/Akt pathways were activated in the MTC patient carrying the M72I variant. In silico results showed conformational changes in H-RAS that could influence its activation by Sos and phosphate binding. Results of molecular dynamics were consistent with Western blot experiments.The M72I mutation may contribute effectively to proliferation and survival signaling throughout the MAPK and PI3K/Akt pathways. This work underscores the importance of studying genetic alterations that may lead to carcinogenesis.
Subject(s)
Carcinoma, Medullary/genetics , Genes, ras/genetics , Mutation/genetics , Thyroid Neoplasms/genetics , Blotting, Western , Carcinoma, Medullary/metabolism , Codon/genetics , DNA/genetics , Exons/genetics , Female , Goiter, Nodular/etiology , Humans , Melanoma/complications , Middle Aged , Mitogen-Activated Protein Kinases/genetics , Mitogen-Activated Protein Kinases/metabolism , Phosphatidylinositol 3-Kinases/genetics , Phosphatidylinositol 3-Kinases/metabolism , Protein Conformation , Proto-Oncogene Mas , Signal Transduction/genetics , Thyroid Neoplasms/metabolismABSTRACT
We report the identification and characterization of a homologue of the IL1RAPL transcript which is responsible for a form of X-linked mental retardation (MRX34). This new transcript was cloned by analysis of genomic sequences from the Xq22 region and was named IL1RAPL2 (Interleukin 1 Receptor Accessory Protein-Like-2). The two X-linked genes share the same domains, the same exon-intron organization and a high degree of similarity at the protein level (70.4% similarity). RNA in situ expression studies on mouse embryo tissue section at different developmental stages show that Il1rapl2 is specifically expressed in the nervous system from embryonic day 12.5. The homologies together with the pattern of expression render ILRAPL2 a candidate gene for disorders displaying involvement of the CNS, including the MRX loci for which the gene has not been identified yet.
Subject(s)
Central Nervous System/metabolism , Receptors, Interleukin-1/genetics , X Chromosome/genetics , Adult , Animals , Blotting, Northern , Brain/metabolism , Chromosome Mapping , DNA, Complementary/chemistry , DNA, Complementary/genetics , Embryo, Mammalian/metabolism , Gene Expression Regulation , Gene Expression Regulation, Developmental , Humans , In Situ Hybridization , Interleukin-1 Receptor Accessory Protein , Mice , Molecular Sequence Data , RNA, Messenger/genetics , RNA, Messenger/metabolism , Sequence Analysis, DNAABSTRACT
We identified new transcribed sequences, using a differential display paradigm to select genes expressed in proliferating neuroblasts from mouse telencephalon at 10 days of embryonic development. In this systematic search, we isolated a 361-bp partial 3' untranslated region (3' UTR) homologous to the 3' UTR of the human gene encoding a putative intracellular kinase regulator, glia maturation factor beta (GMFB). We cloned a full-length, 4,311-bp mouse cDNA containing a 270-bp 5' UTR, a 3,615-bp 3' UTR, and an open reading frame of 426 nucleotides encoding a putative 142 amino-acid protein, identical to human GMFB, with the exception of two amino acids. This 4.3-kb transcript is present in a variety of adult tissues and is developmentally regulated as shown by Northern blot analysis. Differential expression in telencephalon was demonstrated by quantification of radioactive relative RT-PCR and confirmed by in situ hybridization. The isolation of this full-length clone of mouse Gmfb should facilitate investigation of the intracellular mechanisms involved in the development of telencephalon.
Subject(s)
Glia Maturation Factor/genetics , Protein Kinases/metabolism , Telencephalon/metabolism , 3' Untranslated Regions/analysis , 3' Untranslated Regions/genetics , Amino Acid Sequence , Animals , Cloning, Molecular , DNA, Complementary/genetics , Expressed Sequence Tags , Gene Expression Regulation, Developmental , Glia Maturation Factor/chemistry , Glia Maturation Factor/pharmacology , Humans , In Situ Hybridization , Mice , Molecular Sequence Data , RNA, Messenger/analysis , RNA, Messenger/genetics , Sequence Alignment , Sequence Homology , Telencephalon/cytology , Telencephalon/embryology , Telencephalon/enzymologyABSTRACT
During corticogenesis, early-born neurons of the preplate and layer 6 are important for guiding subsequent neuronal migrations and axonal projections. Tbr1 is a putative transcription factor that is highly expressed in glutamatergic early-born cortical neurons. In Tbr1-deficient mice, these early-born neurons had molecular and functional defects. Cajal-Retzius cells expressed decreased levels of Reelin, resulting in a reeler-like cortical migration disorder. Impaired subplate differentiation was associated with ectopic projection of thalamocortical fibers into the basal telencephalon. Layer 6 defects contributed to errors in the thalamocortical, corticothalamic, and callosal projections. These results show that Tbr1 is a common genetic determinant for the differentiation of early-born glutamatergic neocortical neurons and provide insights into the functions of these neurons as regulators of cortical development.
Subject(s)
Cell Adhesion Molecules, Neuronal/metabolism , DNA-Binding Proteins/physiology , Extracellular Matrix Proteins/metabolism , Gene Expression Regulation, Developmental/physiology , Germ Layers/metabolism , Neocortex/embryology , Animals , Cell Death , Cell Movement/physiology , DNA-Binding Proteins/genetics , Lac Operon/physiology , Mice , Mice, Mutant Strains , Mice, Transgenic , Mutation , Neocortex/abnormalities , Neocortex/growth & development , Nerve Tissue Proteins , Neural Pathways/physiology , Neurons/metabolism , Reelin Protein , Serine Endopeptidases , Synaptic Transmission , T-Box Domain ProteinsABSTRACT
Oral-facial-digital type 1 syndrome (OFD1 [MIM 311200]) is transmitted as an X-linked dominant condition with lethality in males and is characterized by malformations of the face, oral cavity, and digits, and by a highly variable expressivity even within the same family. Malformation of the brain and polycystic kidneys are commonly associated with this disorder. The locus for OFD1 was mapped by linkage analysis to a 12-Mb interval, flanked by markers DXS85 and DXS7105 in the Xp22 region. To identify the gene responsible for this syndrome, we analyzed several transcripts mapping to the region and found mutations in OFD1 (formerly named "Cxorf5/71-7a"), encoding a protein containing coiled-coil alpha-helical domains. Seven patients with OFD1, including three with familial and four with sporadic cases, were analyzed. Analysis of the familial cases revealed a missense mutation, a 19-bp deletion, and a single base-pair deletion leading to a frameshift. In the sporadic cases, we found a missense (de novo), a nonsense, a splice, and a frameshift mutation. RNA in situ studies on mouse embryo tissue sections show that Ofd1 is developmentally regulated and is expressed in all tissues affected in OFD1 syndrome. The involvement of OFD1 in oral-facial-digital type I syndrome demonstrates an important role of this gene in human development.
Subject(s)
Abnormalities, Multiple/genetics , Face/abnormalities , Fingers/abnormalities , Mouth Abnormalities/genetics , Mutation , Proteins/genetics , Toes/abnormalities , X Chromosome , Amino Acid Sequence , Base Sequence , Chromosome Mapping , Exons , Female , Humans , Male , Molecular Sequence Data , Pedigree , SyndromeABSTRACT
Citron-kinase (Citron-K) has been proposed by in vitro studies as a crucial effector of Rho in regulation of cytokinesis. To further investigate in vivo its biologic functions, we have inactivated Citron-K gene in mice by homologous recombination. Citron-K-/- mice grow at slower rates, are severely ataxic, and die before adulthood as a consequence of fatal seizures. Their brains display defective neurogenesis, with depletion of specific neuronal populations. These abnormalities arise during development of the central nervous system due to altered cytokinesis and massive apoptosis. Our results indicate that Citron-K is essential for cytokinesis in vivo but only in specific neuronal precursors. Moreover, they suggest a novel molecular mechanism for a subset of human malformative syndromes of the CNS.
Subject(s)
Apoptosis/genetics , Cell Division/genetics , Neurodegenerative Diseases/genetics , Neurons/metabolism , Protein Serine-Threonine Kinases/genetics , Animals , Ataxia/etiology , Brain/embryology , Brain/pathology , Cyclin D1/metabolism , DNA/biosynthesis , Intracellular Signaling Peptides and Proteins , Mice , Mice, Knockout , Neurodegenerative Diseases/complications , Neurodegenerative Diseases/pathology , Neurons/pathology , Polyploidy , Protein Serine-Threonine Kinases/biosynthesis , Protein Serine-Threonine Kinases/deficiency , Seizures/etiology , Stem Cells/metabolism , Stem Cells/pathology , rho-Associated KinasesABSTRACT
We report on the expression pattern of a novel EGF- containing gene named Maeg. RNA in situ studies indicate that Maeg is first activated during specification of the early lateral dermatome, and continues to be expressed in all the dermatome derivatives as the dermis of the trunk, the hair follicles, and the mesenchyme of the cranio-facial region.
Subject(s)
Embryonic and Fetal Development/genetics , Epidermal Growth Factor/genetics , Glycoproteins , Growth Substances , Neoplasm Proteins , Peptides , Animals , Base Sequence , Calcium-Binding Proteins , Cell Adhesion Molecules , DNA Primers/genetics , Gene Expression Regulation, Developmental , Genetic Markers , In Situ Hybridization , Mice , RNA, Messenger/genetics , RNA, Messenger/metabolism , Somites/metabolismABSTRACT
We identified in the EST database murine and human sequences similar, but not identical, to the members of the PC3/BTG/TOB family of cell cycle inhibitors. A conserved domain (aa 50-68) of the PC3 protein, the prototype member of the family, was used as a query. That domain has been shown by us to be necessary for the antiproliferative activity of PC3. A murine EST clone and a highly homologous human EST clone, containing the entire ORF, were chosen for sequencing. Comparison to databases and a phylogenetic tree analysis indicated that these EST clones are the mouse and human homologues of a gene that represents a novel member of the PC3/BTG/TOB family. This gene, named PC3B, is endowed with marked antiproliferative activity, being able to induce G(1) arrest, and is highly expressed in testis, in oocyte, and in preimplantation embryos. Analysis of its expression during murine development indicated a specific localization in the olfactory epithelium at midgestation, suggesting that PC3B might be involved in the differentiation of this neuronal structure. Human PC3B mapped to chromosome 11q23, as indicated by radiation hybrid analysis.
Subject(s)
Cell Cycle Proteins/genetics , Olfactory Mucosa/metabolism , Amino Acid Sequence , Animals , Aspartic Acid Endopeptidases/chemistry , Aspartic Acid Endopeptidases/genetics , Base Sequence , Cell Cycle/genetics , Cell Cycle Proteins/chemistry , Cloning, Molecular , Conserved Sequence , Evolution, Molecular , Expressed Sequence Tags , Humans , Mice , Molecular Sequence Data , Multigene Family , Olfactory Mucosa/cytology , Open Reading Frames , Phylogeny , Proprotein Convertases , Sequence Alignment , Sequence Homology, Amino AcidABSTRACT
Pallial and subpallial morphological subdivisions of the developing chicken telencephalon were examined by means of gene markers, compared with their expression pattern in the mouse. Nested expression domains of the genes Dlx-2 and Nkx-2.1, plus Pax-6-expressing migrated cells, are characteristic for the mouse subpallium. The genes Pax-6, Tbr-1, and Emx-1 are expressed in the pallium. The pallio-subpallial boundary lies at the interface between the Tbr-1 and Dlx-2 expression domains. Differences in the expression topography of Tbr-1 and Emx-1 suggest the existence of a novel "ventral pallium" subdivision, which is an Emx-1-negative pallial territory intercalated between the striatum and the lateral pallium. Its derivatives in the mouse belong to the claustroamygdaloid complex. Chicken genes homologous to these mouse genes are expressed in topologically comparable patterns during development. The avian subpallium, called "paleostriatum," shows nested Dlx-2 and Nkx-2.1 domains and migrated Pax-6-positive neurons; the avian pallium expresses Pax-6, Tbr-1, and Emx-1 and also contains a distinct Emx-1-negative ventral pallium, formed by the massive domain confusingly called "neostriatum." These expression patterns extend into the septum and the archistriatum, as they do into the mouse septum and amygdala, suggesting that the concepts of pallium and subpallium can be extended to these areas. The similarity of such molecular profiles in the mouse and chicken pallium and subpallium points to common sets of causal determinants. These may underlie similar histogenetic specification processes and field homologies, including some comparable connectivity patterns.
Subject(s)
Body Patterning/genetics , Chick Embryo/metabolism , DNA-Binding Proteins/genetics , Gene Expression Regulation, Developmental/physiology , Homeodomain Proteins/genetics , Mice/embryology , Nuclear Proteins/genetics , Telencephalon/embryology , Transcription Factors/genetics , Age Factors , Animals , Chick Embryo/cytology , Cytoskeletal Proteins , Embryo, Mammalian , Eye Proteins , Mice/anatomy & histology , Mice/metabolism , PAX6 Transcription Factor , Paired Box Transcription Factors , RNA, Messenger/metabolism , RNA-Binding Proteins , Repressor Proteins , T-Box Domain Proteins , Telencephalon/cytology , Telencephalon/metabolism , Thyroid Nuclear Factor 1ABSTRACT
The BarH1 and BarH2 ( Bar ) Drosophila genes are homeobox-containing genes, which are required for the fate determination of external sensory organs in the fly. By means of a bioinformatic approach, we have identified in mouse and human two homeobox genes highly related to the Bar Drosophila genes, Barhl1 and Barhl2. While Barhl1 represents a novel gene, Barhl2 turned out to correspond to the mBH1 cDNA recently described in rat. We isolated and sequenced the full-length mouse Barhl1 and mapped both the human BARHL1 and BARHL2 genes to chromosomes 9q34 and 1p22, respectively. Detailed analysis of the murine Barhl1 expression pattern by in situ hybridization revealed that this transcript is exclusively expressed in restricted domains of the developing CNS, which suggests that this gene, similar to its Drosophila counterparts BarH1 and BarH2, may play a crucial role in cell fate determination of neural structures. In particular, Barhl1 showed specific domains of expression in the diencephalon and in the rhombencephalon where it was found to be expressed in migrating cells giving rise to the cerebellar external granular layer and to specific populations of dorsal sensory interneurons of the spinal cord. Thus, Barhl1 function may be required for the generation of these specific subtypes of neuronal progenitors. Furthermore, the mapping assignment and the expression pattern make BARHL1 an attractive positional candidate gene for a form of Joubert syndrome, a rare developmental anomaly of the cerebellum in humans.
Subject(s)
Central Nervous System/metabolism , Genes, Homeobox , Homeodomain Proteins/biosynthesis , Homeodomain Proteins/genetics , Nerve Tissue Proteins/biosynthesis , Nerve Tissue Proteins/genetics , Neurons/metabolism , Amino Acid Sequence , Animals , Central Nervous System/embryology , Cerebellum/abnormalities , Cerebellum/metabolism , Chromosomes, Human, Pair 1 , Chromosomes, Human, Pair 9 , DNA, Complementary/metabolism , Embryo, Mammalian/metabolism , Gene Library , Humans , In Situ Hybridization , Mice , Molecular Sequence Data , Repressor Proteins , Sequence Analysis, DNA , Sequence Homology, Amino Acid , Spinal Cord/metabolismABSTRACT
We isolated a novel mouse gene, RP42, in a systematic search for genes expressed in proliferating neuroblasts whose human orthologs map to susceptibility loci for autism. This gene is intronless and encodes a putative 259-amino-acid protein that exhibits 30-36% overall sequence identity to a fission yeast and a nematode protein (GenPept Accession Nos. CAA17006 and CAB54261). Nevertheless, no homology to any known gene was found. RP42 has developmentally regulated expression, particularly in proliferating neuroblasts from which neocortical neurons originate. Its human ortholog is located in a cluster of embryonic neuronally expressed genes on the 6q16 chromosome, making it a positional candidate susceptibility gene for autism.
Subject(s)
Autistic Disorder/genetics , Chromosomes, Human, Pair 6/genetics , Proteins/genetics , Amino Acid Sequence , Animals , Blotting, Northern , Chromosome Mapping , Cloning, Molecular , DNA, Complementary/chemistry , DNA, Complementary/genetics , Embryo, Mammalian/metabolism , Female , Gene Expression , Gene Expression Regulation, Developmental , Genetic Predisposition to Disease , Humans , Intracellular Signaling Peptides and Proteins , Male , Mice , Molecular Sequence Data , RNA, Messenger/genetics , RNA, Messenger/metabolism , Sequence Alignment , Sequence Analysis, DNA , Sequence Homology, Amino Acid , Tissue DistributionABSTRACT
The signal for somatic sex determination in mammals, Caenorhabditis elegans and Drosophila melanogaster is chromosomal, but the overall mechanisms do not appear to be conserved between the phyla. However it has been found quite recently that the C. elegans sex-determining gene Mab-3 contains a domain highly homologous to the Drosophila sex-determining gene doublesex (dsx) and shares a similar role. These data suggest that at least some aspects of the regulation of sex determination might be conserved. In humans, a doublesex-related gene (DMRT1) was identified at less than 30 kb from the critical region for sex reversal on chromosome 9p24 (TD9). In order to get insights into the role of DMRT1 in sex determination/differentiation, we have isolated DMRT1 mouse homologue (Dmrt1) and analysed its expression pattern. The gene is expressed in the genital ridges of both sexes during the sex-determining switch and it shows male/female dimorphism at late stages of sex differentiation.
Subject(s)
Drosophila Proteins , Transcription Factors/genetics , Amino Acid Sequence , Animals , DNA-Binding Proteins/chemistry , Female , Gene Expression , Humans , Insect Proteins/chemistry , Male , Mice , Molecular Sequence Data , Sequence Homology, Amino Acid , Sex Determination Processes , Sex Differentiation , Transcription Factors/physiologyABSTRACT
We have isolated a human and murine homologue of the Drosophila prune gene through dbEST searches. The gene is ubiquitously expressed in human adult tissues, while in mouse developing embryos a high level of expression is confined to the nervous system particularly in the dorsal root ganglia, cranial nerves, and neural retina. The gene is composed of eight exons and is located in the 1q21.3 chromosomal region. A pseudogene has been sequenced and mapped to chromosomal region 13q12. PRUNE protein retains the four characteristic domains of DHH phosphoesterases. The synergism between prune and awdK-pn in Drosophila has led various authors to propose an interaction between these genes. However, such an interaction has never been supported by biochemical data. By using interaction-mating and in vitro co-immunoprecipitation experiments, we show for the first time the ability of human PRUNE to interact with the human homologue of awd protein (nm23-H1). In contrast, PRUNE is impaired in its interaction with nm-23-H1-S120G mutant, a gain-of-function mutation associated with advanced neuroblastoma stages. Consistently, PRUNE and nm23-H1 proteins partially colocalize in the cytoplasm. The data presented are consistent with the view that PRUNE acts as a negative regulator of the nm23-H1 protein. We discuss how PRUNE regulates nm23-H1 protein and postulate possible implications of PRUNE in neuroblastoma progression.
Subject(s)
Biomarkers, Tumor/genetics , Chromosomes, Human, Pair 13 , Drosophila Proteins , Gene Expression Regulation , Insect Proteins/genetics , Monomeric GTP-Binding Proteins , Transcription Factors/genetics , Adult , Amino Acid Sequence , Animals , Antigens, Neoplasm/genetics , Chromosome Mapping , Drosophila , Gene Expression Regulation, Developmental , Humans , Mice , Molecular Sequence Data , NM23 Nucleoside Diphosphate Kinases , Nucleoside-Diphosphate Kinase/genetics , Sequence Alignment , Sequence Homology, Amino AcidABSTRACT
Using a bioinformatic approach, we have identified a new transcript, SLC7A8, mapping to 14q11.2, within the lysinuric protein intolerance (LPI) critical region. This gene is highly expressed in skeletal muscle, intestine, kidney, and placenta and encodes a predicted protein of 535 amino acids, homologous to the amino acid permease CD98 light chain and cationic amino acid transporters. RNA in situ hybridization data on mouse embryos confirm the expression in kidney and intestine and, interestingly, reveal that SLC7A8 is also highly expressed in eye, in retinal pigmented epithelium, and in tooth buds at day 16.5 of gestation. Mutational analysis excluded any direct involvement of the SLC7A8 gene product in LPI disease. The homology data and the expression pattern are in agreement with the hypothesis that SLC7A8 represents a novel light chain interacting with the 4F2 heavy chain in the multimeric complex mediating neutral and/or cationic amino acid transport and cystine/glutamate exchange.
Subject(s)
Amino Acid Metabolism, Inborn Errors/genetics , Carrier Proteins/chemistry , Carrier Proteins/genetics , Lysine/urine , Membrane Proteins/chemistry , Membrane Proteins/genetics , Multigene Family , Amino Acid Metabolism, Inborn Errors/etiology , Amino Acid Metabolism, Inborn Errors/urine , Amino Acid Sequence , Amino Acid Transport Systems, Basic , Animals , Antigens, CD/genetics , Carrier Proteins/isolation & purification , Contig Mapping , DNA, Complementary/isolation & purification , Fusion Regulatory Protein-1 , Genetic Markers , Humans , Membrane Proteins/isolation & purification , Mice , Molecular Sequence Data , Nucleic Acid Hybridization , Rats , Sequence Alignment , Sequence Homology, Amino Acid , XenopusABSTRACT
We describe the identification and characterization of a new gene deleted in the AMME contiguous gene syndrome. This gene is predominantly expressed in heart, skeletal muscle, spinal cord, and brain. Screening of placenta and NT2 cDNA libraries enabled us to obtain the 1.5-kb full-length transcript, which shows a 426-bp open reading frame. Since the resulting 142-amino-acid peptide has a single putative transmembrane domain and a weak but suggestive homology with KCNE1 (minK), a protein associated with the KCNQ1 potassium channel (KVLQT1), we named this new gene KCNE1-like (KCNE1L). To obtain greater insight into this new member of an apparently distinct protein family, we have identified and characterized the homologous mouse gene (Kcne1l), which encodes a peptide of 143 amino acids with 91% homology and 80% identity. The expression pattern of mouse Kcne1l in the developing embryo revealed strong signal in ganglia, in the migrating neural crest cells of cranial nerves, in the somites, and in the myoepicardial layer of the heart. The specific distribution in adult tissues, the putative channel function, and the expression pp6tern in the developing mouse embryo suggest that KCNE1L could be involved in the development of the cardiac abnormalities as well as of some neurological signs observed in patients with AMME contiguous gene syndrome.
Subject(s)
Gene Deletion , Intellectual Disability/genetics , Nephritis, Hereditary/genetics , Potassium Channels, Voltage-Gated , Potassium Channels/genetics , X Chromosome/genetics , Animals , Chromosome Mapping , Clone Cells , Databases, Factual , Electric Conductivity , Electrocardiography , Gene Expression , Heart Defects, Congenital/genetics , Humans , In Situ Hybridization , Male , Membrane Proteins/genetics , Mice , Molecular Sequence Data , Potassium Channels/chemistry , Sequence Homology, Nucleic Acid , SyndromeABSTRACT
Herein, the genetic control of regionalization and connectivity of the neocortex are reviewed. Evidence is accumulating which suggests that intrinsic mechanisms have a central role in controlling cortical regional specification and differentiation. Expression patterns of several genes (Id-2, Tbr-1, cadherin-6, cadherin-8, neuropilin-2, Wnt-7b, Eph-A7 and RZR-beta) are described; the expressions of these genes have regional boundaries which demarcate distinct functional areas of the cerebral cortex in neonatal mice.
Subject(s)
Gene Expression Regulation, Developmental , Glycoproteins , Neocortex/cytology , Neocortex/embryology , Neural Pathways/cytology , Neural Pathways/embryology , Repressor Proteins , Transcription Factors , Animals , Animals, Newborn , Axons/chemistry , Axons/physiology , Biomarkers , Brain Stem/cytology , Cadherins/genetics , DNA-Binding Proteins/genetics , Inhibitor of Differentiation Protein 2 , Mice , Mice, Inbred Strains , Neocortex/growth & development , Nerve Tissue Proteins/genetics , Neural Pathways/growth & development , Neuropilin-1 , Proto-Oncogene Proteins/genetics , RNA, Messenger/analysis , Receptor Protein-Tyrosine Kinases/genetics , Receptor, EphA7 , Receptors, Cell Surface/genetics , Receptors, Cytoplasmic and Nuclear/genetics , Receptors, Melatonin , Spinal Cord/cytology , T-Box Domain Proteins , Thalamus/cytology , Wnt ProteinsABSTRACT
We have identified a transcription factor specifically expressed in the developing vertebrate eye. We named this gene vax2 because of the high degree of sequence similarity to the recently described vax1. Both in the human and mouse genomes, vax2 is localized in the vicinity of the emx1 gene. This mapping assignment, together with the previously reported colocalization of Vax1 and Emx2 in mouse, indicates that the vax and the emx genes may be organized in clusters. vax2 has a remarkable expression domain confined to the ventral portion of the prospective neural retina in mouse, human, and Xenopus. The overexpression of either the frog Xvax2 or the human VAX2 in Xenopus embryos leads to an aberrant eye phenotype and, in particular, determines a ventralizing effect on the developing eye. The expression domain of the transcription factor Xpax2, normally confined to the ventral developing retina, extends to the dorsal region of the retina after overexpression of vax2. On the other hand, the expression of Xvent2, a molecular marker of the dorsal retina, is strongly reduced. Furthermore, vax2 overexpression induces a striking expansion of the optic stalk, a structure deriving from the ventralmost region of the eye vesicle. Altogether, these data indicate that vax2 plays a crucial role in eye development and, in particular, in the specification of the ventral optic vesicle.
Subject(s)
Eye/embryology , Homeodomain Proteins/genetics , Homeodomain Proteins/metabolism , Homeodomain Proteins/physiology , Xenopus Proteins , Amino Acid Sequence , Animals , Body Patterning/genetics , DNA, Complementary/analysis , Eye/metabolism , Gene Expression Regulation, Developmental , Genetic Linkage , Humans , In Situ Hybridization , Larva , Mice , Molecular Sequence Data , Phenotype , Physical Chromosome Mapping , Retina/embryology , Retina/metabolism , Sequence Homology, Amino Acid , Time Factors , Tissue Distribution , XenopusABSTRACT
The members of the T-box gene family share a highly conserved DNA binding domain named the T-domain, and important developmental functions. Here we report the cloning of chicken Tbr1 and of murine and chicken Tbr2 (orthologs of the Xenopus eomesodermin gene), the mapping of the murine Tbr2 to chromosome 9, and their pattern of expression during mouse and chick embryogenesis. Both Tbr 1 and 2 have a restricted and conserved domain of expression in the telencephalic pallium of the two species. Chick Tbr2 has a specific and dynamic expression in the gastrulating embryo.
Subject(s)
Brain/embryology , DNA-Binding Proteins/genetics , Gene Expression Regulation, Developmental , Nerve Tissue Proteins , T-Box Domain Proteins , Amino Acid Sequence , Animals , Brain/growth & development , Brain/metabolism , Chick Embryo , Chickens , Chromosome Mapping , Cloning, Molecular , DNA-Binding Proteins/metabolism , Mice , Mice, Inbred C57BL , Molecular Sequence Data , Sequence Homology, Amino Acid , Transcription Factors/geneticsABSTRACT
The general strategies of phototransduction in vertebrates and invertebrates share many similarities, but differ significantly in their underlying molecular machinery. The CDS gene encodes the CDP-diacylglycerol synthase (CDS) enzyme and is required for phototransduction in Drosophila. Using a bioinformatic approach, we have identified two novel transcripts (CDS1 and CDS2) highly homologous to the Drosophila CDS gene. We isolated and sequenced the CDS2 full-length cDNA and mapped the two genes to human chromosomes 20p13 (CDS2) and 4q21.1 (CDS1). Sequence analysis revealed that both genes are highly homologous to the Drosophila protein (64.4 and 58. 6% identity at the protein level between CDS and CDS2 and between CDS and CDS1, respectively). The mouse homologs for both genes were isolated and used in RNA in situ hybridization studies on adult and embryonic mouse tissue sections. These studies showed that Cds2 is highly expressed in the differentiating neuroblasts of the neural retina and in the central nervous system during embryonic development, while it was not detected in adult retina. Cds1, on the other hand, shows a high level of expression in the photoreceptor layer of adult retina, which strongly suggests a role for Cds1 in phototransduction. Knowledge of the expression pattern of these genes in mammals may shed light on the evolution of vision mechanisms and help in the evaluation of candidate genes for human retinopathies.
Subject(s)
Diacylglycerol Cholinephosphotransferase/genetics , Adult , Amino Acid Sequence , Animals , Chromosome Mapping , Chromosomes, Human, Pair 20 , Chromosomes, Human, Pair 4 , Drosophila , Gene Expression , Humans , In Situ Hybridization, Fluorescence , Mice , Mice, Inbred C57BL , Molecular Sequence Data , Retina/embryology , Retina/enzymology , Retina/metabolism , Vision, OcularABSTRACT
We have isolated a family of four vertebrate genes homologous to eyes absent (eya), a key regulator of ocular development in Drosophila. Here we present the detailed characterization of the EYA4 gene in human and mouse. EYA4 encodes a 640 amino acid protein containing a highly conserved C-terminal domain of 271 amino acids which in Drosophila eya is known to mediate developmentally important protein-protein interactions. Human EYA4 maps to 6q23 and mouse Eya4 maps to the predicted homology region near the centromere of chromosome 10. In the developing mouse embryo, Eya4 is expressed primarily in the craniofacial mesenchyme, the dermamyotome and the limb. On the basis of map position and expression pattern, EYA4 is a candidate for oculo-dento-digital (ODD) syndrome, but no EYA4 mutations were found in a panel of ODD patients.