1.
Int J Oncol
; 18(1): 181-5, 2001 Jan.
Article
in English
| MEDLINE
| ID: mdl-11115557
ABSTRACT
We developed a rapid, sensitive and robust high risk human papillomavirus (HR HPV) detection protocol based on direct in situ PCR technology and fluorochrome-modified nucleotides on cytologic specimens (cell smears) and on HPV infected tissues (CIN III). Reproducible results on both cytologic specimens and paraffin-embedded tissues were obtained, providing a powerful tool for clinical investigation on HR HPV infection. Quantitative PCR performed on the same tissue sections adjacent to those used for in situ techniques allowed us to establish the sensitivity of our methods, able to detect rare copies (about 15 in our paraffin-embedded tissues) of HPV.
Subject(s)
Papillomaviridae/isolation & purification , Polymerase Chain Reaction/methods , Uterine Cervical Dysplasia/virology , Female , Formaldehyde , HeLa Cells , Humans , In Situ Hybridization, Fluorescence/methods , Papillomaviridae/genetics , Paraffin Embedding , Risk Factors , Sensitivity and Specificity
2.
Eur J Histochem
; 43(2): 155-7, 1999.
Article
in English
| MEDLINE
| ID: mdl-10439218