ABSTRACT
Abuse of antibiotics and their residues in the environment results in the emergence and prevalence of drug-resistant bacteria and leads to serious health problems. Herein, a photon-controlled antibacterial platform that can efficiently kill drug-resistant bacteria and avoid the generation of new bacterial resistance was designed by encapsulating black phosphorus quantum dots (BPQDs) and pharmaceuticals inside a thermal-sensitive liposome. The antibacterial platform can release pharmaceuticals in a spatial-, temporal-, and dosage-controlled fashion because the BPQDs can delicately generate heat under near-infrared light stimulation to disrupt the liposome. This user-defined delivery of drug can greatly reduce the antibiotic dosage, thus avoiding the indiscriminate use of antibiotics and preventing the generation of superbugs. Moreover, by coupling the photothermal effect with antibiotics, this antibacterial platform achieved a synergistic photothermal-/pharmaco-therapy with significantly improved antibacterial efficiency toward drug-resistant bacteria. The antibacterial platform was further employed to treat antibiotic-resistant bacteria-caused skin abscess and it displayed excellent antibacterial activity in vivo, promising its potential clinical applications. Additionally, the antibacterial mechanism was further investigated. The developed photon-controlled antibacterial platform can open new possibilities for avoiding bacterial resistance and efficiently killing antibiotic-resistant bacteria, making it valuable in fields ranging from antiinfective therapy to precision medicine.
Subject(s)
Anti-Bacterial Agents , Hyperthermia, Induced , Infrared Rays , Methicillin-Resistant Staphylococcus aureus/growth & development , Phototherapy , Quantum Dots , Staphylococcal Skin Infections , Animals , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacokinetics , Anti-Bacterial Agents/pharmacology , Liposomes , Mice , Quantum Dots/chemistry , Quantum Dots/therapeutic use , Staphylococcal Skin Infections/metabolism , Staphylococcal Skin Infections/pathology , Staphylococcal Skin Infections/therapyABSTRACT
Treatment of bacterial infections due to the fast emergence of drug-resistant bacteria is a significant challenge faced in modern medicine. Here the authors report a drug-induced self-assembly nanoantibiotic for treating bacterial infection, with temperature/pH-sensitivity, synergistic antibacterial effect of silver and antibiotics, and immunoregulatory effect. In this nanoantibiotic, smart polymer p(N-isopropylacrylamide-b-acrylic acid) triblock polymer (PNA) utilized to encapsulate the drugs provides convenience in preparing this structure simply through drug-induced self-assembly and controllable release profile by changing the sequence of addition of different drugs. The polymer also allows the nanoantibiotic to be responsive to multiple external stimuli such as pH, temperature, and ionic strength. The silver and antibiotics codelivered in this nanoantibiotic can exert a synergistic antibacterial effect due to the different antibacterial mechanisms. More importantly, macrophages can be activated into an M2 phenotype to promote tissue repair by this nanoantibiotic for the negative surface charge and the antibiotics contained. The self-assembly nanoantibiotic exhibited great promise to be applied in the treatment of bacterial infection and provide favorable utility for inflammation treatment, tissue engineering, and targeted therapy.