Twelve-point scale grading system of scanning electron microscopic examination to investigate subtle changes in damaged hair surface.

BACKGROUND: To assess the hair surface condition, scanning electron microscope (SEM) is commonly used and it remains an indispensable hair morphology characterization technique. Yet, the technique is criticized for having subjective viewpoints and limitations in distinguishing the appearance of cuticle layers. OBJECTIVES: The aim of this study is to establish an objective classification system and also to subdivide by detailed description of damaged cuticle layers. METHODS: Hair samples were collected from female subjects (n = 500) who participated in hair efficacy study and Asian hair bunches (n = 180) that were previously collected. Damage to hair was initiated by chemical, heat stress and ultraviolet irradiation. We suggested the grading criterion on a 12-point scale and compared with a wide range grading system on a 5-point scale. We evaluated other hair surface-related parameters such as hair luster-ring and combing load to verify the validity and efficacy of our new grading system. RESULTS: The grading criterion on our 12-point scale revealed an improved discrimination compared to the wide range grading system. Hair surface-related parameters were significantly improved after hair care product, and these tendencies were likely to be determined to be similarly improved using the 12-point scale grading system. CONCLUSION: The 12-point scale classification system was demonstrated to be a more precise standardization and appropriate evaluation method to investigate the subtle distinction of the hair shaft after hair care product application.

Facial skin physiology recovery kinetics during 180 min post-washing with a cleanser.

BACKGROUND/PURPOSE: Facial cleansing is important to clean and exfoliate the skin while maintaining optimal physiologic function. However, there is insufficient data on the very early stage of skin change after applying soap or cleansing foam. We investigated the recovery kinetics of facial skin physiology during 180 min after exposure to the cleanser. METHODS: For the study, 22 Korean female subjects with normal and dry to oily skin type were recruited in this study. Study subjects were required to have face washing done within the 12 hours prior to visiting the research center, with only toner, lotion, or cream applied. The next day, the subjects visited the research center without face washing. We evaluated the skin hydration (Corneometer(®) CM 825), sebum (Sebumeter(®) SM 815), transepidermal water loss (Tewameter(®) TM 300), and pH (Skin-pH-Meter(®) PH 905) to define recovery kinetics of facial skin physiology during 180 min exposure post-cleansing. RESULTS: Skin hydration, sebum, and TEWL were significantly decreased at 20 min after washing, as compared to the baseline (P < 0.05). And skin hydration returned at 40 min, and skin sebum and TEWL returned at 120 min after washing. However, skin pH did not show significant differences at all times points. CONCLUSIONS: This study indicated that each of the skin parameters was restored at defined time points post-cleansing. Our result could be a useful reference to set the resting time in the estimation of skin bioengineering parameters.

The effects of statins on the clinical outcomes of Clostridium difficile infection in hospitalised patients.

BACKGROUND: An association between exposure to statin drugs and favourable treatment outcomes for various types of infections has been established. AIM: To determine the clinical characteristics and treatment outcomes of Clostridium difficile infection (CDI) among hospitalised patients taking statin drugs. METHODS: The medical records were reviewed for consecutive in-patients with CDI confirmed by positive toxin assay (A or B), C. difficile culture, or the presence of pseudomembrane on endoscopy. Treatment success was defined as the resolution of diarrhoea within 6 days of therapy. The primary end points were assessed by average symptom recovery time and treatment response (success or failure). RESULTS: Among 949 patients, the overall response to metronidazole was 91.9%. The baseline characteristics showed some differences between statin users and statin non-users with respect to mean disease severity score. In the multivariate analysis, successful treatment response was significantly associated with the absence of exposure to proton pump inhibitors (PPIs) (OR = 0.690, 95% CI = 0.513-0.929, P = 0.014) and with exposure to statins (OR = 1.449, 95% CI = 1.015-2.070, P = 0.041). Contrary to the treatment response, univariate and multivariate analyses failed to show that exposure to PPIs or statins affected symptom recovery times. Sixty-day CDI recurrence rates for those patients with statin exposure were significantly lower compared with those patients without statin exposure (3% vs. 7.3%, respectively; RR = 0.393, 95% CI = 0.167-0.926, P = 0.033). CONCLUSION: Prior statin exposure in patients with C. difficile infection is associated with a successful response to treatment.