Subject(s)
Celiac Disease/complications , Celiac Disease/immunology , Cerebellar Diseases/immunology , Demyelinating Autoimmune Diseases, CNS/immunology , Adolescent , Adult , Aged , Autoantibodies/analysis , Autoantibodies/blood , Celiac Disease/physiopathology , Cerebellar Diseases/diagnosis , Cerebellar Diseases/physiopathology , Cerebellar Nuclei/physiopathology , Cerebellum/immunology , Cerebellum/physiopathology , Demyelinating Autoimmune Diseases, CNS/physiopathology , Female , Glutens/immunology , Humans , Male , Middle Aged , Neurologic Examination , Ocular Motility Disorders/diagnosis , Ocular Motility Disorders/immunology , Ocular Motility Disorders/physiopathology , Reflex, Vestibulo-Ocular/immunology , Saccades/immunology , Young AdultABSTRACT
A bibliographical search was conducted for papers published between 1999 and 2007 to verify the validity of International Classification of Headache Disorders (ICHD)-II criteria for the Tolosa-Hunt syndrome (THS) in terms of (i) the role of magnetic resonance imaging (MRI); (ii) which steroid treatment should be considered as adequate; and (iii) the response to treatment. Of 536 articles, 48, reporting on 62 patients, met the inclusion criteria. MRI was positive in 92.1% of the cases and it normalized after clinical resolution. There was no evidence of which steroid schedule should be considered as adequate; high-dose steroids are likely to be more effective both to induce resolution and to avoid recurrences. Pain subsided within the time limit required by the ICHD-II criteria, but signs did not. We conclude that THS diagnostic criteria can be improved on the basis of currently available data. MRI should play a pivotal role both to diagnose and to follow-up THS.
Subject(s)
International Classification of Diseases , Orbital Cellulitis/diagnosis , Orbital Cellulitis/drug therapy , Practice Guidelines as Topic/standards , Sinusitis/diagnosis , Sinusitis/drug therapy , Tolosa-Hunt Syndrome/diagnosis , Tolosa-Hunt Syndrome/drug therapy , Cavernous Sinus , Humans , Internationality , Orbital Cellulitis/epidemiology , Outcome Assessment, Health Care/methods , Prognosis , Sinusitis/epidemiology , Tolosa-Hunt Syndrome/epidemiology , Treatment OutcomeABSTRACT
Swallowing problems can be relevant, even if underestimated, in Multiple Sclerosis (MS) patients. However, no specific questionnaire for the assessment of dysphagia in MS is available. We built a questionnaire (DYsphagia in MUltiple Sclerosis, DYMUS) that was administered to 226 consecutive MS patients (168 F, 58 M, mean age 40.5 years, mean disease duration 10.1 years, mean EDSS 3.1) during control visits in four Italian MS Centres. DYMUS was abnormal in 80 cases (35%). The patients who claimed to have swallowing problems had a significantly higher mean DYMUS score that the other patients (p<0.0001). Mean DYMUS scores were significantly higher in the progressive forms (p=0.003). DYMUS values were significantly correlated to EDSS (p=0.0007). DYMUS showed a very good internal consistency (Cronbach's alpha 0.877). Factor analysis allowed us to sub-divide DYMUS in two sub-scales, 'dysphagia for solid' and 'dysphagia for liquid', both of them had a very good internal consistency (Cronbach's alpha 0.852 and 0.870 respectively). DYMUS demonstrated to be an easy and consistent tool to detect dysphagia and its main characteristics in MS. It can be used for preliminary selection of patients to submit to more specific instrumental analyses, and to direct toward programs for prevention of aspiration.
Subject(s)
Deglutition Disorders/diagnosis , Deglutition Disorders/etiology , Multiple Sclerosis/complications , Surveys and Questionnaires , Adult , Aged , Deglutition Disorders/epidemiology , Female , Humans , Italy , Male , Middle Aged , Multiple Sclerosis/epidemiology , Reproducibility of Results , Sensitivity and SpecificityABSTRACT
OBJECTIVE: Mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE-HS) may involve extrahippocampal areas of structural and functional damage. The incidence and the features of this damage are still a matter of debate and vary depending on the method applied. Memory guided saccades (MGSs) with a memorization delay longer than 20s can be used reliably to evaluate the parahippocampal cortex. METHODS: MGSs with 3 and 30s memorization delays were recorded with the search coil technique in six patients affected by right MTLE-HS, and in 13 healthy controls. RESULTS: The patients were not able to reduce the MGSs residual amplitude error after the first saccade with a 30s memorization delay. This finding was more evident with leftward saccades. CONCLUSIONS: MGS abnormalities suggested the functional involvement of the right parahippocampal cortex in most of the patients with MTLE-HS, and this supports the clinical and anatomopathological heterogeneity of the disease. SIGNIFICANCE: MGSs can be used in patients with right MTLE-HS to detect a possible functional involvement of the ipsilateral parahippocampal cortex.
Subject(s)
Epilepsy, Temporal Lobe/complications , Epilepsy, Temporal Lobe/physiopathology , Hippocampus/pathology , Memory/physiology , Saccades/physiology , Adult , Anticonvulsants/therapeutic use , Electroencephalography , Eye Movements/physiology , Female , Functional Laterality/physiology , Humans , Magnetic Resonance Imaging , Male , Middle Aged , Parahippocampal Gyrus/physiopathology , SclerosisABSTRACT
Guillain-Barré syndrome (GBS) is an autoimmune acute peripheral neuropathy. Frequently a flu-like episode or a gastroenteritis precede GBS, and the cross-reactivity between microbial and neural antigens partly explains the pathophysiology of the disease and the possible detection of antiganglioside antibodies. The weakness reaches its nadir in 2-4 weeks: the patients may be chair- or bed-bound, may need artificial ventilation and frequently experience dysautonomic dysfunction; 5-15% of the patients die and more patients are left with a disabling motor deficit and/or fatigue. Electrophysiology and cerebrospinal fluid evaluation support the diagnosis. The treatment of GBS is multidisciplinary, and both plasma exchange and high dose immunoglobulin (IVIg) are effective in reducing both the severity of the disease and the residual deficits. Finally, steroids are not effective in GBS.
Subject(s)
Guillain-Barre Syndrome , Guillain-Barre Syndrome/epidemiology , Guillain-Barre Syndrome/pathology , Guillain-Barre Syndrome/therapy , HumansABSTRACT
The geographical analysis of a disease risk is particularly difficult when the disease is non-frequent and the area units are small. The practical use of the Bayesian modelling, instead of the classical frequentist one, is applied to study the geographical variation of multiple sclerosis (MS) across the province of Pavia, Northern Italy. 464 MS-affected individuals resident in the province of Pavia were identified on December 31st 2000. The overall prevalence was 94 per 100,000 inhabitants. This estimate indicates an increasing MS prevalence in the province, in accordance with the vast majority of the Italian areas where prevalence studies have been repeated. We mapped the geographical variation of MS prevalence across the 190 communes of the province both with a classical approach and a Bayesian approach. The frequentist approach produced an extremely dishomogeneous map, while the Bayesian map was much smoother and more interpretable. Our study underlines the usefulness of Bayesian methods to obtain reliable maps of disease prevalence and to identify possible clusters of disease where to carry out further epidemiological investigations.
Subject(s)
Multiple Sclerosis/epidemiology , Adolescent , Adult , Aged , Bayes Theorem , Cluster Analysis , Cross-Sectional Studies , Epidemiologic Methods , Female , Humans , Italy/epidemiology , Male , Middle Aged , PrevalenceABSTRACT
OBJECTIVE: Paired serum and cerebrospinal fluid (CSF) specimens were investigated for Chlamydia pneumoniae-specific oligoclonal bands (OCBs) to determine band specificity in multiple sclerosis (MS). MATERIAL AND METHODS: Serum and CSF samples were collected from patients with relapsing-remitting MS (n = 56), other inflammatory (n = 18) or non-inflammatory (n = 15) neurologic diseases, and from 10 healthy controls. OCBs were determined with affinity immunoblotting of C. pneumoniae-specific IgG onto antigen-coated nitrocellulose paper after protein separation with agarose isoelectric focusing. RESULTS: Chlamydia pneumoniae-specific OCBs were present in 5 of 56 patients with MS, and in 3 of 18 patients with other inflammatory neurologic diseases. CONCLUSIONS: The intrathecal production of C. pneumoniae-specific oligoclonal IgG occurs in a minority of patients with MS. This intrathecal anti-C. pneumoniae reactivity is likely part of a polyspecific humoral immune response in MS.
Subject(s)
Chlamydophila Infections/immunology , Chlamydophila pneumoniae/immunology , Multiple Sclerosis/immunology , AIDS Dementia Complex/complications , AIDS Dementia Complex/immunology , Adolescent , Adult , Aged , Aged, 80 and over , Antigens, Bacterial/immunology , Chlamydophila Infections/complications , Female , Humans , Immunoblotting , Male , Middle Aged , Multiple Sclerosis/complications , Neurosyphilis/complications , Neurosyphilis/immunology , Oligoclonal Bands/blood , Oligoclonal Bands/cerebrospinal fluidABSTRACT
We studied repeated cerebrospinal fluids of patients with Devic's neuromyelitis optica (NMO) and multiple sclerosis (MS). Variations of oligoclonal bands (OBs) had opposite trends in the two groups. In MS, OBs were detected in 399 of 411 patients (97%) and never disappeared. In NMO, OBs were detected in three of 11 patients (27%) and always disappeared. The hypothesis that NMO and MS follow distinct pathogenetic pathways is supported by our findings, which can be useful for the differentiation of NMO from MS.
Subject(s)
Multiple Sclerosis/cerebrospinal fluid , Neuromyelitis Optica/cerebrospinal fluid , Oligoclonal Bands/cerebrospinal fluid , Adult , Diagnosis, Differential , Female , Humans , Male , Middle Aged , Multiple Sclerosis/diagnosis , Neuromyelitis Optica/diagnosis , Retrospective StudiesABSTRACT
Intracellular cytokine flow cytometry was used to analyse the percentages of interferon (IFN) gamma and interleukin (IL)-4 producing T cells in the peripheral blood of multiple sclerosis patients, before and after immunomodulatory treatment, and of healthy controls. After six months of treatment, different doses of IFN beta1a (Avonex or Rebif) decreased CD4+ (Th1, Th2) and CD8+ (Tc1) cells to a similar extent, without affecting the Th1/Th2 ratio. These T cell subsets were unmodified after nine months of glatiramer acetate (Copaxone) treatment, and after six day courses of high dose 6-methylprednisolone. The data suggest that IFN beta1a produces sustained downmodulation of IFN gamma and IL-4 producing T cells in vivo, which may contribute to its therapeutic efficacy; that glatiramer acetate possibly acts without altering non-specific cellular immunity; and that glucocorticoid induced lymphocytopenia does not affect the percentages of Th1, Th2, and Tc1 cells; at least in the periphery, none of the treatments caused a Th1 to Th2 shift that could account for their respective therapeutic effects.
Subject(s)
Adjuvants, Immunologic/therapeutic use , Interferon-beta/therapeutic use , Interferon-gamma/biosynthesis , Interleukin-4/biosynthesis , Multiple Sclerosis, Relapsing-Remitting/immunology , T-Lymphocytes/immunology , Adult , CD4-Positive T-Lymphocytes/immunology , CD4-Positive T-Lymphocytes/metabolism , CD8-Positive T-Lymphocytes/immunology , CD8-Positive T-Lymphocytes/metabolism , Female , Flow Cytometry , Glatiramer Acetate , Glucocorticoids/therapeutic use , Humans , Interferon beta-1a , Male , Methylprednisolone/therapeutic use , Middle Aged , Multiple Sclerosis, Relapsing-Remitting/blood , Multiple Sclerosis, Relapsing-Remitting/drug therapy , Peptides/therapeutic use , Reference Values , T-Lymphocyte Subsets/immunology , T-Lymphocyte Subsets/metabolism , T-Lymphocytes/metabolism , Th1 Cells/immunology , Th1 Cells/metabolism , Th2 Cells/immunology , Th2 Cells/metabolismABSTRACT
OBJECTIVE: To detect disconjugate ocular motor abnormalities and a possible extraocular muscle myotonic phenomenon in patients with myotonic dystrophy (MyD). METHODS: The magnetic scleral search coil technique was used to record monocularly the small (25 degrees ) and large (50 degrees ) saccades, which were paced to two interstimulus intervals (ISIs), one short (1 s), the other long (5 s). The case study comprised 20 patients with MyD, 10 patients with multiple sclerosis (MS), and 10 controls. The amplitude, duration, peak velocity, and skewness of the velocity profile (ratio between the acceleration and the deceleration periods) of each saccade were measured. The disconjugate parameters (difference between the two eyes of the same measure), and the myotonic parameter (the maximal (as absolute value) short-long ISI difference between the same measures) were considered. RESULTS: The disconjugate parameters were the same in all three groups. The mean values of myotonic parameters found in patients with MyD for duration (for both small and large target displacements) and skewness (for small target displacements only) differed from those found for both the MS and the control groups. Additionally, the occurrence of individual patients presenting with abnormal duration and skewness parameters was higher in the MyD than in the MS group. In patients with MyD, the saccade duration was longer for long than for short ISI; the effect derived from a prolongation of the acceleration period, which manifested as an increase in skewness. CONCLUSION: The results can be explained by a combination of the myotonic and the warm up phenomena. A delay in the relaxation (myotonia) of the extraocular muscle may be more evident after a long fixation period (long ISI) and it may improve by increasing saccade pacing (short ISI-warm up). This phenomenon is slight, and is unlikely to affect saccade performance significantly, but it may provide some insight into the nature of the disorder affecting extraocular and skeletal muscles in myotonic dystrophy.
Subject(s)
Myotonic Dystrophy/diagnosis , Oculomotor Nerve Diseases/diagnosis , Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Multiple Sclerosis/diagnosis , Multiple Sclerosis/physiopathology , Myotonic Dystrophy/physiopathology , Oculomotor Muscles/physiopathology , Oculomotor Nerve Diseases/physiopathology , Reaction Time/physiology , Saccades/physiologyABSTRACT
With the aid of a Bayesian statistical model of the natural course of relapsing remitting Multiple Sclerosis (MS), we identify short-term clinical predictors of long-term evolution of the disease, with particular focus on predicting onset of secondary progressive course (failure event) on the basis of patient information available at an early stage of disease. The model specifies the full joint probability distribution for a set of variables including early indicator variables (observed during the early stage of disease), intermediate indicator variables (observed throughout the course of disease, prefailure) and the time to failure. Our model treats the intermediate indicators as a surrogate response event, so that in right-censored patients, these indicators provide supplementary information pointing towards the unobserved failure times. Moreover, the full probability modelling approach allows the considerable uncertainty which affects certain early indicators, such as the early relapse rates, to be incorporated in the analysis. With such a model, the ability of early indicators to predict failure can be assessed more accurately and reliably, and explained in terms of the relationship between early and intermediate indicators. Moreover, a model with the aforementioned features allows us to characterize the pattern of disease course in high-risk patients, and to identify short-term manifestations which are strongly related to long-term evolution of disease, as potential surrogate responses in clinical trials. Our analysis is based on longitudinal data from 186 MS patients with a relapsing-remitting initial course. The following important early predictors of the time to progression emerged: age; number of neurological functional systems (FSs) involved; sphincter, or motor, or motor-sensory symptoms; presence of sequelae after onset. During the first 3 years of follow up, to reach EDSS> or =4 outside relapse, to have sphincter or motor relapses and to reach moderate pyramidal involvement were also found to be unfavourable prognostic factors.
Subject(s)
Bayes Theorem , Multiple Sclerosis/epidemiology , Age of Onset , Cohort Studies , Disease Progression , Female , Humans , Italy/epidemiology , Male , Markov Chains , Models, Neurological , Multiple Sclerosis/complications , Multiple Sclerosis/therapy , Optic Neuritis/epidemiology , Optic Neuritis/etiology , Paralysis/epidemiology , Paralysis/etiology , Prognosis , Remission, Spontaneous , Risk Factors , Treatment Failure , Urinary Incontinence/epidemiology , Urinary Incontinence/etiologyABSTRACT
With an Italian case series of 81 Italian patients and 130 controls, we analysed associations between myasthenia gravis (MG) and genetic polymorphisms in the MHC class II/III region. Increases in the frequency of the TNF-B*1, C4A*Q0, C4B*1, DRB1*03 supratype, which is likely part of the 8.1 ancestral haplotype, were maximal in females with early onset (EO) MG vs. controls [p<0.05, relative risk (RR)=9.9]. These patients showed neither a significantly high frequency of thymic hyperplasia, nor high levels of serum anti-acethylcholine receptor antibodies. The DRB1*03 allele was absent in patients with thymoma; however, in comparison with controls, occurrence of this marker was frequent in MG patients (p<0.005; RR=6.2), more frequent in females (p<0.005; RR=7.8) and most frequent in EOMG female patients (p<0.005; RR=15.1). Analysis of the TNF-B*1, C4A*Q0, C4B*1, DRB1*03 supratype and its recombinants showed that the MHC region between C4 and TNF might contain genes that influence susceptibility to MG in females. Polymorphic markers within the supratype, e.g. TNF-B*1 and C4A*Q0, might contribute to pathogenetically significant abnormalities in immune responses in a subset of female MG patients. The combined effect of other intervening genes cannot be excluded.
Subject(s)
Genes, MHC Class II/immunology , Myasthenia Gravis/genetics , Myasthenia Gravis/immunology , Polymorphism, Genetic/genetics , Adult , Aged , Chromosome Mapping , Complement C4a/genetics , Complement C4b/genetics , Complement Factor B/genetics , DNA Mutational Analysis , Deoxyribonucleases, Type II Site-Specific/genetics , Female , Gene Deletion , Gene Frequency , Genetic Markers/physiology , Genetic Predisposition to Disease/genetics , Genetic Testing , Genotype , Humans , Italy , Lymphotoxin-alpha/genetics , Male , Middle Aged , Myasthenia Gravis/blood , Sex CharacteristicsABSTRACT
The two chemokines, monocyte chemoattractant protein (MCP)-1 and gamma-interferon inducible protein (IP)-10, are thought to be involved in the pathogenesis of multiple sclerosis (MS). We measured MCP-1 and IP-10 levels in serum and CSF samples from 38 acute and 25 stable MS patients and from 40 controls. The latter consisted in patients with other inflammatory neurological diseases (OIND) or with non-inflammatory neurological diseases, and healthy controls. CSF MCP-1 levels exceeded those found in serum in all the patients studied as well as in healthy controls. CSF MCP-1 levels were significantly lower in acute MS [468+/-(S.E.M.) 18 pg/ml] than in stable MS (857+/-104 pg/ml). When detectable, serum and CSF IP-10 levels were significantly higher in acute MS (serum 331+/-66 pg/ml; CSF 118+/-16 pg/ml) than in stable MS (serum 69+/-7 pg/ml; CSF 25+/-2 pg/ml). Among OIND patients, those with HIV-1-associated dementia showed high serum and CSF levels of both MCP-1 and IP-10. Those with encephalitis showed high serum and CSF levels of IP-10 and CSF mononuclear pleiocytosis. We also evaluated the effects of 6-methylprednisolone or IFN-beta1a therapy on circulating MCP-1 and IP-10 levels. Neither MCP-1 nor IP-10 post-therapy levels varied significantly from baseline values. Our findings suggest that (a) MCP-1 could be constitutively produced within the brain; (b) MCP-1 and IP-10 CSF levels in acute MS vary significantly from those in stable MS, and these variations are inverse; and (c) current MS therapies do not modify circulating levels of MCP-1 and IP-10.
Subject(s)
Adjuvants, Immunologic/therapeutic use , Chemokine CCL2/metabolism , Chemokines, CXC/metabolism , Multiple Sclerosis/blood , Multiple Sclerosis/cerebrospinal fluid , Acute Disease , Adult , Aged , Anti-Inflammatory Agents/therapeutic use , Chemokine CXCL10 , Female , Humans , Interferon beta-1a , Interferon-beta/therapeutic use , Male , Methylprednisolone/therapeutic use , Middle Aged , Multiple Sclerosis/therapy , Severity of Illness Index , Treatment OutcomeABSTRACT
Developmental reading disability (dyslexia) has traditionally been attributed to impaired linguistic skills. Recent psychophysical data suggest that dyslexia may be related to a visual perceptual deficit. A few visual evoked potential (VEP) studies have addressed this hypothesis, but their results are far from consistent. We submitted 9 dyslexic subjects and 9 age- and sex-matched normal controls to checkerboard pattern reversal VEPs. The main experimental variables were: large (0.5 cycles per degree; cpd) and small (2 cpd) checks and two reversal frequencies (2.1 Hz and 8 Hz); mean luminance and contrast (60 cd/m2 and 50%, respectively) were kept constant in all four conditions. Transient VEP (2.1 Hz) parameters did not differ between controls and dyslexics at 2 cpd. At 0.5 cpd, N70 amplitude was significantly smaller and N70 latency significantly shorter in dyslexics. Amplitudes for the fundamental frequency (8 Hz), as well as for the second and third harmonics of the steady-state VEPs were smaller in dyslexics for both stimulus sizes. A discriminant analysis correctly classified each subject. Our data confirm the hypothesis of a perceptual deficit in dyslexic subjects. The abnormalities are related to spatial and temporal stimulus frequencies: they appear when large stimuli are presented, or when the stimulation frequency is high. These data support the hypothesis of selective magnocellular dysfunction in dyslexia.
Subject(s)
Dyslexia/physiopathology , Evoked Potentials, Visual/physiology , Adolescent , Child , Electroencephalography , Female , Humans , Male , Pattern Recognition, Visual/physiology , Photic Stimulation , ReadingABSTRACT
Vestibular evoked myogenic potentials (VEMPs) are myogenic responses induced by stimulation of the saccular macula by intense sound stimuli. The responses are recordable from the sternocleidomastoid (SCM) muscles. We recorded VEMPs from normal subjects (up to three times in each subject) to identify: i) the best recording procedures, ii) the reliability, and iii) the normal limits for both individual point and test-retest evaluation. We adopted a recording setting in which the subjects were asked to simultaneously activate both SCM muscles by pushing their forehead against a load cell during a bilateral acoustic stimulation. This system enabled subjects to monitor their intensity of SCM activation and to keep intensity constant; us to record VEMPs from both sides simultaneously, and thus to minimize the duration of the recording session. For each subject we considered the mean and the difference (divided by the mean) of the values derived from the two SCM muscles of the latency of the P13 and N23 components and of the P13-N23 peak-to-peak amplitude. Reliability was evaluated by estimate of the intraclass correlation coefficient, and was good or excellent for all parameters, with the exception of the P13-N23 amplitude side-difference. To take advantage of all the data available, we computed the normal limits for both individual point and test-retest evaluation by means of the variability indices used for the evaluation of reliability. In this system, VEMP recording is simple, inexpensive and rapid. It is well tolerated by subjects, and easily implemented in laboratories equipped for evoked potential recording.
Subject(s)
Evoked Potentials/physiology , Muscle, Skeletal/physiology , Vestibule, Labyrinth/physiology , Electromyography/methods , Humans , Models, Biological , Reproducibility of ResultsABSTRACT
Pro- and anti-inflammatory cytokines are thought to participate in the development and regulation of autoimmunity in multiple sclerosis (MS), a demyelinating disease of the central nervous system (CNS). We analysed the percentage of interferon (IFN)-gamma and interleukin (IL)-4-producing cells in the peripheral blood of both active and stable MS patients, and of healthy controls. After short-term stimulation, cytokine-producing cells were intracellularly stained and sorted. Significantly lower percentages of IFN-gamma and IL-4-producing T cells were found in stable MS patients than in controls, and in active than in stable patients. The diminution affected CD4(+) (Th1, Th2) and CD8(+) (Tc1) phenotypes. Tc2 cells were not detected. The Th1/Th2 ratio did not differ in active and stable MS, nor in controls. The fact that Th2 and Tc1 cell percentages were higher in stable than in active MS possibly indicates that these cells play a downmodulating role in the immune response. In contrast, a role in exacerbating the immune response is not attributable to Th1 cells, given their reduction in acute MS. Our data do not support the hypothesis that MS is a Th1/Th2 paradigmatic disease; rather, they suggest that sequestration in the CNS, or activation-induced apoptosis (whether in vivo or in vitro) may explain reduced levels of IFN-gamma and IL-4-producing subsets in the peripheral blood of clinically acute patients.
Subject(s)
Interferon-gamma/biosynthesis , Interleukin-4/biosynthesis , Multiple Sclerosis/immunology , Th1 Cells/immunology , Th2 Cells/immunology , Adolescent , Adult , Female , Humans , Lymphocyte Activation , Male , Middle Aged , Recurrence , T-Lymphocyte Subsets/classificationABSTRACT
OBJECTIVES: Body cooling has been proposed as a symptomatic treatment for multiple sclerosis. This study aimed to assess the effects of body cooling and of circadian variations on clinical parameters and on visual and auditory evoked potential measures in multiple sclerosis patients. METHODS: Clinical status was assessed and VEPs, BAEPs and MLAEPs (all with two stimulus frequencies) were recorded a total of 4 times on two separate days (two times per day at 08:30 and 15:00 h each day) in 10 multiple sclerosis patients and 10 controls. On one of these days, the subjects were submitted to body cooling before the afternoon session. RESULTS: Tympanic temperature was significantly higher in the afternoon. Cooling lowered the temperature by 1.4 degrees C. No clinical effects were observed. Circadian effects were detected on VEP amplitude, which increased both in controls and in patients at low stimulus frequency (P<0.01), and increased in controls and decreased in patients at high stimulus frequency (interaction: P<0.01). Cooling determined an increase in BAEP I-V peak-to-peak time in controls, and a reduction in patients at high stimulus frequency (interaction: P<0.01). In patients, cooling also determined a great increase in MLAEP amplitude (interaction: P<0.001). We did not find cooling effects on VEP measures. CONCLUSIONS: Visual and auditory evoked potentials showed differences in circadian and cooling effects between controls and multiple sclerosis patients. These differences are consistent with the hypothesis of temperature-dependent conduction blocks in demyelinated fibers. Cooling may have a clinical effect in selected patients only.
Subject(s)
Brain/physiopathology , Circadian Rhythm/physiology , Evoked Potentials, Auditory/physiology , Evoked Potentials, Visual/physiology , Hypothermia, Induced , Multiple Sclerosis/physiopathology , Acoustic Stimulation , Adult , Electroencephalography , Female , Humans , Male , Photic StimulationABSTRACT
OBJECTIVE: Periodic alternating nystagmus has been associated with the instability of the velocity storage mechanism, which is known to play an important role in both the vestibulo-oculomotor and the optokinetic systems. In the present study we looked for a possible spinal equivalent to PAN. METHODS AND RESULTS: In 3 PAN patients, the H-reflex amplitude proved to be slightly but significantly influenced by nystagmus direction, in that it was greater when the nystagmus was beating toward the stimulation side. CONCLUSIONS: This finding suggests that projections from velocity storage may play a role not only in the ocular motor but also in assisting postural stability through the vestibulo-spinal system.