ABSTRACT
We experimentally demonstrate generation of a squeezed vacuum at 800 nm with a Sagnac loop fiber interferometer. When negative dispersion is properly added to an input laser pulse to compensate for the fiber dispersion, the level of squeezing is improved. A squeezed vacuum of 0.45 dB is obtained at a dispersion of -0.0157 ps(2) for the 1.5 m-long fiber loop. Since the squeezed vacuum is degraded by guided acoustic-wave Brillouin scattering (GAWBS), the noise level of the squeezing is improved by -0.3 dB at a liquid nitrogen temperature. We also demonstrate generation of photon number squeezing at -1.3 dB.
Subject(s)
Interferometry/methods , Lasers , Acoustics , Amplifiers, Electronic , Equipment Design , Models, Statistical , Models, Theoretical , Nitrogen , Optical Fibers , Photons , Scattering, Radiation , Spectrum Analysis, Raman/methodsSubject(s)
Cerebral Infarction/etiology , Fibroma/diagnosis , Heart Neoplasms/diagnosis , Mitral Valve , Echocardiography, Transesophageal , Fibroma/complications , Heart Neoplasms/complications , Heart Valve Diseases/complications , Heart Valve Diseases/diagnosis , Humans , Male , Middle Aged , Tomography, X-Ray ComputedABSTRACT
OBJECTIVES: To study the involvement of vasospasm as the trigger of acute myocardial infarction without significant stenosis, the circadian variation of the time of onset of acute myocardial infarction was compared with that of vasospastic angina without significant stenosis. METHODS: The subjects consisted of 3 groups, 64 patients with acute myocardial infarction without significant stenosis, 101 patients with acute myocardial infarction with one vessel disease and 98 patients with vasospastic angina without significant stenosis. The times of onset of acute myocardial infarction and spontaneous attack of vasospastic angina were recorded and classified according to the 4 periods (0:00-6:00, 6:00-12:00, 12:00-18:00, 18:00-24:00) and the pattern of distribution was compared between the 3 groups. RESULTS: The time distribution of acute myocardial infarction without significant stenosis showed a double peaked pattern at 6:00-12:00 and 18:00-24:00 and was similar to the pattern of acute myocardial infarction with one vessel disease(p = 0.93). Only a single peak in 0:00-6:00 was found in the pattern of distribution of vasospastic angina without significant stenosis and differed significantly from acute myocardial infarction(p < 0.01). CONCLUSIONS: The circadian variation of the time of onset of acute myocardial infarction was similar in patients with or without significant stenosis, and differed significantly from that in patients with vasospastic angina.
Subject(s)
Angina Pectoris/epidemiology , Circadian Rhythm , Coronary Vasospasm/complications , Myocardial Infarction/epidemiology , Angina Pectoris/complications , Female , Humans , Male , Middle Aged , Myocardial Infarction/etiology , TimeABSTRACT
We previously demonstrated that GLUT2, a facilitated-diffusion glucose transporter isoform known to play critical roles in the regulation of systemic blood glucose level, is present at the apical ends of Müller cells in the rat retina. As a means of elucidating the ontogeny and possible role(s) of GLUT2 in the developing retina, this study examined its expression at various stages of retinal development by immunofluorescence staining using GLUT2-specific antibody. Evidence of GLUT2 expression first appeared at embryonic day 14 (E14) as linear staining along the boundary between the inner and outer layers of the optic cup, with this staining pattern being present throughout subsequent embryonic and neonatal stages. After the development of photoreceptor cell inner and outer segments (i.e., photoreceptor layer), GLUT2 immunoreactivity was localized along the boundary between the outer nuclear layer and photoreceptor layer. Localization of GLUT2 expression and the timing of its appearance, which coincided with the formation of choriocapillaries, together suggest that GLUT2 is involved in the anterior transport of glucose supplied by choroidal circulation from the early stages of retinal development.
Subject(s)
Monosaccharide Transport Proteins/analysis , Retina/chemistry , Retina/embryology , Animals , Capillaries/cytology , Epithelial Cells/cytology , Fluorescent Antibody Technique , Glucose Transporter Type 2 , Pigment Epithelium of Eye/embryology , Pigment Epithelium of Eye/ultrastructure , Rats , Rats, Sprague-Dawley , Retina/ultrastructure , Retinal Vessels/cytologyABSTRACT
To determine whether the site and morphology of coronary artery spasm provoked with acetylcholine can predict the long-term prognosis of vasospastic angina, coronary artery spasm (more than 90% narrowing) provoked with acetylcholine was studied in 66 consecutive patients (56 males, 10 females, mean age 56 +/- 9 years) with vasospastic angina. All patients were followed for 6.7 +/- 0.9 years and the incidence of cardiac events such as sudden death, myocardial infarction or worsened unstable angina was compared with the site and morphology of provoked spasm. The site of spasm was regarded as proximal when spasm occurred in the proximal site of 3 major coronary arteries which was designated as segment 1, 6 or 11, according to the classification of the American Heart Association, and distal in other segments. The morphology of spasm was classified into 3 types, focal (12 cases, localized more than 90% narrowing with adjoining parts constricting less than 25%), diffuse (17 cases, diffuse more than 90% narrowing), and intermediate (37 cases, localized more than 90% narrowing with adjoining parts constricting 25-90%). The site of spasm was classified into 2 types, the proximal group (24 cases) and the distal group (42 cases). Cardiac events occurred in 7 patients during the follow-up period: sudden death in 2, myocardial infarction in 2, and worsened unstable angina in 3. As to the site of spasm, the incidence of cardiac events was 21% (5/24 patients) in the proximal group, significantly higher than 5% (2/42) in the distal group (p < 0.05). As to the site of spasm, the incidence of cardiac events was 41% (5/12) in the focal group, significantly higher than 3% (1/37) in the intermediate group and 6% (1/17) in the diffuse group (p < 0.001). The presence of proximal and focal coronary artery spasm was associated with a significantly higher incidence of cardiac events. The site and morphology of coronary artery spasm provoked with acetylcholine is related to the long-term prognosis of vasospastic angina.
Subject(s)
Angina Pectoris/complications , Coronary Vasospasm/complications , Acetylcholine , Aged , Angina, Unstable/diagnosis , Angina, Unstable/physiopathology , Coronary Angiography , Coronary Vasospasm/physiopathology , Death, Sudden, Cardiac , Female , Follow-Up Studies , Humans , Male , Middle Aged , Myocardial Infarction/etiology , Prognosis , Vasodilator AgentsABSTRACT
Myelin is synthesized about the time of birth. The Src-family tyrosine kinase Fyn is involved in the initial events of myelination. Fyn is present in myelin-forming cells and is activated through stimulation of cell surface receptors such as large myelin-associated glycoprotein (L-MAG). Here we show that Fyn stimulates transcription of the myelin basic protein (MBP) gene for myelination. MBP is a major component of the myelin membrane. In 4-week-old Fyn-deficient mice, MBP is significantly reduced, and electron microscopic analysis showed that myelination is delayed, compared with wild-type mice. The Fyn-deficient mice had thinner, more irregular myelin than the wild-type. We found that Fyn stimulates the promoter activity of the MBP gene by approximately sevenfold. The region responsible for the transactivation by Fyn is located between nucleotides -675 and -647 with respect to the transcription start site. Proteins binding to this region were found by gel shift study, and the binding activity correlates with Fyn activity during myelination. These results suggest that transactivation of the MBP gene by Fyn is important for myelination.
Subject(s)
Myelin Basic Protein/biosynthesis , Myelin Basic Protein/genetics , Myelin Sheath/physiology , Protein-Tyrosine Kinases/biosynthesis , Proto-Oncogene Proteins/biosynthesis , Transcription, Genetic/genetics , Amino Acid Sequence , Animals , Brain Chemistry , Mice , Microscopy, Electron , Molecular Sequence Data , Nuclear Proteins/metabolism , Protein Binding , Proto-Oncogene Proteins c-fyn , Signal Transduction/physiology , Spinal Cord/physiologyABSTRACT
Regulation of adhesion and degranulation of mast cells plays an important role in allergy and inflammation. We investigated a possible role of Bruton's tyrosine kinase (Btk) in the regulation of adhesion and degranulation by using bone marrow-derived mast cells from X-linked immunodeficiency (Xid) and Btk-deficient mice. Cross-linking of the high affinity IgE receptor (Fc epsilonRI) and steel factor (SLF) induced indistinguishable adhesive responses of mast cells to fibronectin in kinetics, and these adhesive responses were comparable among wild type, Xid, and Btk-deficient mast cells. Cross-linking of Fc epsilonRI, but not SLF triggered degranulation of bone marrow-derived mast cells. However, Fc epsilonRI-induced degranulation was impaired in Xid and Btk-deficient mast cells. Calcium influx induced by Fc epsilonRI cross-linking and SLF were also reduced in Xid and Btk-deficient mast cells. Degranulation and calcium influx were reduced more severely in Btk-deficient than in Xid mast cells. Consistently, cross-linking Fc epsilonRI and SLF augmented Btk kinase activities transiently. Inositol triphosphate (IP3) production was also severely reduced in Btk-deficient mast cells, indicating Btk play a critical role of Fc epsilonRI-induced IP3 production. The differential sensitivity of wortmannin on calcium influx in wild type and Xid mast cells suggested that the activation of phosphatidylinositol 3 kinase (PI 3-kinase) was required in calcium influx. Furthermore, abnormal secretory granules with translucent contents and variable in size were observed both in Xid and Btk-deficient mast cells. Our study demonstrated a critical role of Btk in regulating intracellular calcium and granule exocytosis.
Subject(s)
Calcium/metabolism , Cell Degranulation , Immunologic Deficiency Syndromes/immunology , Mast Cells/physiology , Protein-Tyrosine Kinases/metabolism , Agammaglobulinaemia Tyrosine Kinase , Androstadienes/pharmacology , Animals , Blotting, Western , Bone Marrow Cells/physiology , Cell Adhesion/physiology , Enzyme Activation , Enzyme Inhibitors/pharmacology , Female , Inositol 1,4,5-Trisphosphate/metabolism , Mast Cells/ultrastructure , Mice , Mice, Inbred BALB C , Microscopy, Electron , Phosphatidylinositol 3-Kinases/drug effects , Phosphatidylinositol 3-Kinases/metabolism , Precipitin Tests , Receptors, IgE/metabolism , Stem Cell Factor/metabolism , WortmanninABSTRACT
The retinal pigment epithelium of the vertebrate eye plays a major part in the maintenance of ocular function. To identify molecules involved in the exertion of their physiological functions, monoclonal antibodies against bovine retinal pigment epithelial cells were made. Analysis by immunofluoresence and immunoelectron microscopy showed that one of the monoclonal antibodies named anti-RPE7 stained the cell surface of retinal pigment epithelial cells and Müller cells. The anti-RPE7 antibody was revealed to recognize molecules of 45-55 kDa by Western blot analysis. Molecular cloning of the RPE7 cDNA and sequence analysis of the amino acids revealed that protein RPE7 belonged to the Ig superfamily. The high homology of RPE7 with metalloproteinase inducer suggests that the protein RPE7 might play a role in the matrix (interphotoreceptor matrix and basement membranes) remodeling as well as in retinal pigment epithelial cell migration under pathological conditions.
Subject(s)
Antigens, CD , Antigens, Neoplasm , Avian Proteins , Blood Proteins , Immunoglobulins/analysis , Membrane Glycoproteins/analysis , Retina/chemistry , Amino Acid Sequence , Animals , Antibodies, Monoclonal/isolation & purification , Antigens, Surface/genetics , Base Sequence , Basigin , Blotting, Northern , Blotting, Western , Cattle , Cells, Cultured , Immunoglobulins/chemistry , Immunoglobulins/genetics , Membrane Glycoproteins/chemistry , Membrane Glycoproteins/genetics , Microscopy, Fluorescence , Microscopy, Immunoelectron , Molecular Sequence Data , Pigment Epithelium of Eye/chemistry , Pigment Epithelium of Eye/cytology , Retina/cytology , Sequence AlignmentABSTRACT
This is a case of a 70-year-old woman with thymic cyst which was found by chance on admission for treatment of acute bronchial asthma and diagnosed by pathological examination. After remission of the asthma she was asymptomatic and no pathological physical findings were found except of cervical thyroid tumor of adenomatous goiter. Chest X-ray films revealed a large mediastinal mass with a sharp margin at the right cardiophrenic angle. Chest CT and MRI revealed that it occupied a space from upper anterior mediastinum to right cardiophrenic angle, and that it showed low density area without enhancement, suggesting a mediastinal cyst. A multilocular cyst, 15 cm in diameter, containing serous liquid was excised using thoracotomy. Pathological and immunohistochemical examination of the cyst revealed that its inner surface was lined with benign cylindrical epithelium and that thymic tissues existed in the walls of the cyst, resulting in a thymic cyst. The thymic cyst, of which reports have increased, should be considered in the differential diagnosis of anterior mediastinal cysts.
Subject(s)
Mediastinal Cyst/pathology , Aged , Diagnosis, Differential , Female , Humans , Mediastinal Cyst/diagnostic imaging , Radiography, Thoracic , Staining and Labeling , Tomography, X-Ray ComputedABSTRACT
The reproducibility of coronary vasospasm was assessed in nine patients with complete remission of vasospastic angina by medical treatment by reexamination at intervals of mean [+/-SD] 5.7 +/- 0.9 years. Twenty-one segments were defined as spastic, demonstrating more than 90% narrowing after acetylcholine injection at the initial angiography. The degree of spasticity, type of spasm (diffuse or focal) and coronary artery diameter in these segments at the initial and follow-up studies were compared. Of the 21 segments, 17 (81%) still had some spasticity (> 25%) at the follow-up study and 8 (38%) of these 17 showed spasticity with greater than 90% narrowing. On the other hand, spasm was not reprovoked in 4 (19%) segments. Luminal diameter of the spastic segments decreased significantly at the follow-up study (2.52 +/- 0.83 vs 2.26 +/- 0.62 mm, p = 0.01), but percentage stenosis was not different between the initial and follow-up studies (9.1 +/- 7.2 vs 10.3 +/- 8.0%, NS). The reproducibility of the type of spasm provoked was 83%. Coronary vasospasticity persists to some extent in spite of complete remission of angina by medical treatment, and the type of spasm provoked has high reproducibility. Therefore, the cessation of drug treatment should be done carefully.
Subject(s)
Angina Pectoris/drug therapy , Coronary Vasospasm/etiology , Acetylcholine , Aged , Calcium Channel Blockers/therapeutic use , Coronary Angiography , Coronary Vasospasm/drug therapy , Female , Follow-Up Studies , Humans , Male , Middle Aged , Recurrence , Remission InductionABSTRACT
Drosophila corneal lens secretion was studied by electron microscope autoradiography of [3H]amino acids (leucine, lysine, phenylalanine, proline and tyrosine) and [3H]sugars (glucosamine and mannose) in newly emerged flies. Ommatidial lenses were homogeneously labelled with both tracers at low levels, suggesting that lens materials turn over continuously after lens formation is completed. In contrast, ocellar lenses were heavily labelled, indicating that deposition of ocellar lens cuticle is still active at this stage. [3H]amino acids and [3H]sugars were deposited in distinct patterns in ocelli. Although over 90% of [3H] sugars remained, even after 3 h after application, within 1 micron of the apices of corneagenous cells associated with lens bases, [3H]amino acids distributed diffusely. There was an obvious gradient of [3H]sugars from center to periphery of the lens base, suggesting that structure of the corneal lens in dorsal ocelli is determined by spatially regulated secretion of chitin by corneagenous cells.
Subject(s)
Autoradiography/methods , Drosophila melanogaster/ultrastructure , Lens, Crystalline/metabolism , Lens, Crystalline/ultrastructure , Microscopy, Electron/methods , Animals , Cornea , Lens, Crystalline/diagnostic imaging , RadiographyABSTRACT
We previously developed a reaggregate cell culture system (pellet cultures) in which retinal neuroepithelial cells proliferate and give rise to rod photoreceptor cells (rods) in vitro (Watanabe and Raff, 1990, Neuron 4:461-467). In the present study, we analyzed cell differentiation and morphogenesis in pellet cultures by using both cell-type-specific markers with immunofluorescence and electron microscopy. We demonstrated that, in addition to rods, the other major retinal cell types, including amacrine cells, bipolar cells, Müller cells, and ganglion cells were all present in the pellets, where most were able to develop from dividing precursor cells in vitro. The different cell types in the pellets became organized into two distinct structures: dark rosettes and pale rosettes. The cellular composition of these structures indicated that the dark rosettes correspond to the outer nuclear layer and the pale rosettes to the inner nuclear layer of the normal retina. Ultrastructural studies have indicated that the thin layer of neuronal processes surrounding the dark rosettes correspond to the outer plexiform layer, and the central region of the pale rosettes correspond to the inner plexiform layer of the normal retina. Other features of normal retinal development also occurred in the pellets, including programmed cell death and the formation of inner and outer rod cell segments and synapses. Thus, pellet cultures provide a convenient way to study different aspects of retinal development where one can control the size and the cellular composition of the initial reaggregate.
Subject(s)
Cell Differentiation/physiology , Retina/growth & development , Retina/ultrastructure , Animals , Cells, Cultured , Female , Microscopy, Electron , Pregnancy , Rats , Rats, Sprague-DawleyABSTRACT
The relationship between coronary vasospasticity and the development of atherosclerotic lesion was studied in 24 patients with vasospastic angina. All patients had no organic stenosis initially and underwent follow-up coronary angiography at 66 +/- 9 months after the initial examination. The coronary artery diameter was measured with the contour detection method. The spastic and non-spastic sites were identified at the initial coronary angiography with the acetylcholine provocation test. The change of the luminal diameter (delta LD) and the ratio of the change of luminal diameter (% delta LD) were compared at the spastic and the non-spastic sites. The follow-up examination showed significant decreases of coronary artery diameter in both the spastic (2.35 +/- 0.67 vs 2.16 +/- 0.58 mm, p < 0.001) and non-spastic sites (2.66 +/- 0.91 vs 2.54 +/- 0.84 mm, p = 0.02). However, delta LD and % delta LD were not different between the spastic and non-spastic sites (delta LD: -0.19 +/- 0.40 vs -0.12 +/- 0.46 mm, NS; % delta LD: -6.7 +/- 14.8% vs -3.2 +/- 17.0%, NS). In conclusion, coronary vasospasticity does not promote the development of atherosclerotic lesion.
Subject(s)
Coronary Vasospasm/pathology , Coronary Vessels/pathology , Aged , Angina Pectoris, Variant/diagnostic imaging , Angina Pectoris, Variant/pathology , Coronary Angiography , Coronary Vasospasm/diagnostic imaging , Female , Follow-Up Studies , Humans , Male , Middle AgedABSTRACT
This study investigates the presence, localization, and developmental expression of a neuron-specific facilitated-diffusion glucose transporter, GLUT3, in the rat retina so as to elucidate molecular mechanisms regulating glucose homeostasis in support of the visual function. Immunoblot analysis using anti-GLUT3 antibody (ALM3-C) revealed the presence of GLUT3 as a heterogeneously glycosylated protein with an average molecular weight of approximately 44 kDa. Although immunofluorescence staining showed it to be localized primarily in the inner and outer plexiform layers, some of the cell bodies in the inner nuclear layer also showed weak immunoreactivity. Immunoblot analysis of developing rat retinal tissues revealed the presence of the GLUT3 protein as early as embryonic day 15 (E15), and immunofluorescence staining revealed its expression in the inner plexiform layer near the time of birth and in the outer plexiform layer at postnatal day 14 (P14), i.e., when the eyes normally open and retinal activity commences. The protein's abundance remained at a relatively low level during the embryonic stages and up until the end of the first postnatal week (P7), though a transient increase was confirmed to occur at E18. From P13, however, the abundance steadily increased, rapidly reaching the adult level at P24. Based on these observations, we hypothesize that GLUT3 is expressed in some subsets of retinal neurons, being preferentially abundant in their neuronal processes, and that its ontogeny is closely associated with morphological and functional development of the retina. As such, this suggests that GLUT3 plays some important role(s) in the retina where glucose metabolism is essential.
Subject(s)
Monosaccharide Transport Proteins/biosynthesis , Nerve Tissue Proteins , Neurons/chemistry , Retina/embryology , Retina/metabolism , Animals , Antibody Specificity , Female , Fluorescent Antibody Technique, Direct , Gene Expression Regulation, Developmental/physiology , Glucose Transporter Type 3 , Immunoblotting , Male , Monosaccharide Transport Proteins/genetics , Monosaccharide Transport Proteins/immunology , Neurons/metabolism , Pregnancy , Rats , Rats, Sprague-Dawley , Retina/cytologyABSTRACT
We studied the structure of ocellar photoreceptor cells of Drosophila melanogaster, particularly the subrhabdomeric cisternae which our previous studies have shown to be essential structures for turnover of photoreceptive membranes in compound eyes. Each ocellus contained elongated photoreceptor cells with rhabdomeres positioned distally. In the subrhabdomeric regions, endocytotic invaginations were frequently observed, suggesting active turnover of photoreceptive membranes. In the vicinity of the photoreceptive microvilli, membranous structures similar to the subrhabdomeric cisternae in compound eyes were observed. These membranous structures were immunopositive for the rdgB protein, a phosphatidylinositol transfer protein that is localized to the subrhabdomeric cisternae in compound eyes. The ocellar photoreceptor cells of the retinal degeneration mutants (rdgA,B) were also studied. In these mutants, retinal degeneration has been reported to start, in compound eyes, with the disappearance of the subrhabdomeric cisternae. We found that the ocellar subrhabdomeric cisternae also disappear during the initial stage of retinal degeneration. From these observations, we conclude that the mechanism of photoreceptive membrane turnover in ocellar photoreceptor cells involves the rdgB and probably the rdgA proteins which are associated with subrhabdomeric cisternae, as is the case for photoreceptive membrane turnover in compound eyes.
Subject(s)
Drosophila melanogaster/anatomy & histology , Membrane Proteins , Photoreceptor Cells, Invertebrate/ultrastructure , Animals , Carrier Proteins/genetics , Genes, Insect , Microscopy, Electron , Microvilli/ultrastructure , Mutation , Phosphatidylinositols/metabolism , Phospholipid Transfer Proteins , Retina/cytology , Retinal Degeneration/metabolismABSTRACT
We demonstrated bundle formation of microtubules both in the cytoplasmic processes and in the cytoplasm near the nucleus of cultured megakaryocytes by means of electron and immunofluorescent microscopy. To determine whether this bundle formation was related to megakaryocyte maturation, we studied the effects of recombinant human interleukin-6 (rhIL-6) in vitro and in vivo on this event. About 75% of the megakaryocytes in the bone marrow had no fibrous microtubule structures in the cytoplasm (type I), and about 25% had bundles of microtubules (type II). The formation of these bundles was promoted by rhIL-6 both in vitro and in vivo. Considerably more megakaryocytes cultured with recombinant murine (rm) IL-3 and rhIL-6 became type II than those cultured with rmIL-3 alone. Megakaryocytes from mice given rhIL-6 (10 microg/animal/d) subcutaneously also began to form bundles in proportion to an increase in platelet counts. After the administration of rhIL-6, about half of the megakaryocytes contained microtubule bundles in their cytoplasm. These results indicate that microtubule-bundle formation is one maturational event in megakaryocyte development and that rhIL-6 could accelerate this event.
Subject(s)
Megakaryocytes/cytology , Microtubules/ultrastructure , Animals , Blood Platelets/ultrastructure , Bone Marrow Cells , Cell Differentiation/drug effects , Cells, Cultured , Cytoplasm/ultrastructure , Female , Humans , Interleukin-3/pharmacology , Interleukin-6/pharmacology , Megakaryocytes/drug effects , Megakaryocytes/ultrastructure , Mice , Microscopy, Electron , Microscopy, Fluorescence , Recombinant Proteins/pharmacology , Tubulin/analysisABSTRACT
The morphology and permeability to horseradish peroxidase (HRP) of the ciliary muscle capillaries of squirrel monkeys were studied. The endothelial cells of the capillaries were of the continuous type and the interendothelial clefts were closed by a zonula occludens. Many vesicles were present in the cytoplasm of the endothelial cells. In addition to these vesicles, large vacuole-like structures, 100-500 nm in diameter, were observed in the cytoplasm of capillary endothelium, especially in the circular and radial ciliary muscles. When HRP was perfused into the anterior chamber, the 3,3'-diaminobenzidine reaction product was observed in the lumen of the ciliary muscle vessels after 30 minutes. When HRP was administered intravenously, the reaction product was observed among the muscle fiber bundles after 15 minutes. In both cases, the reaction products were also present in the cytoplasmic vesicles and vacuole-like structures, suggesting that these structures are involved in the bidirectional transport of HRP between interstitium and blood.
Subject(s)
Capillary Permeability/physiology , Ciliary Body/blood supply , Saimiri/physiology , Animals , Capillaries/ultrastructure , Horseradish Peroxidase/pharmacokineticsABSTRACT
In order to understand the molecular basis of glucose regulation supporting visual function, this study examined the presence of GLUT2, a facilitated-diffusion glucose transporter isoform, and delineated its localization in the rat retina. Reverse transcriptase-polymerase chain reaction (RT-PCR) demonstrated the presence of GLUT2 mRNA, and immunoblot analysis using polyclonal antibody specific to rat GLUT2 revealed a band at a molecular weight of approximately 60 kDa, indicating the presence of GLUT2 protein in the rat retina. Fluorescence and electron microscopy localized GLUT2 expression to the apical ends of Müller cells that face the inter-photoreceptor space. These findings suggest that GLUT2 on Müller cells may control intra-retinal glucose homeostasis by performing both anterior and posterior glucose transport within the rat retina. This is the first study to provide evidence that GLUT2 is present in the mammalian central nervous system and indicates that GLUT2 may have local glucose homeostatic functions within the retina in addition to its role in the regulation of systemic blood glucose level.
Subject(s)
Monosaccharide Transport Proteins/biosynthesis , Nerve Endings/metabolism , Retina/metabolism , Animals , Blotting, Southern , Fluorescent Antibody Technique , Glucose/metabolism , Glucose Transporter Type 2 , Immunoblotting , Immunohistochemistry , Microscopy, Electron , Nerve Endings/ultrastructure , Polymerase Chain Reaction , RNA, Messenger/biosynthesis , Rats , Rats, Sprague-Dawley , Retina/cytology , Retina/ultrastructureABSTRACT
Distribution of rdgB protein, which was recently shown to be a Drosophila phosphatidylinositol transfer protein, was studied in the photoreceptor cells of compound eyes of normal and rdgAPC47 mutant of Drosophila melanogaster by immunoelectron microscopy using (1) pre-embedding HRP staining, (2) pre-embedding NANOGOLD labeling followed by siliver enhancement, (3) and post-embedding colloidal gold labeling methods. In normal cells, immunoreactivity was localized to the membranes of subrhabdomeric cisternae (SRC) and adjacent plasma membranes at the bases of photoreceptive microvilli. In rdgAPC47 mutant cells, whose SRC gradually degenerate, the immunoreactivity in the plasma membranes at the bases of microvilli disappeared in parallel with the degeneration of SRC. Instead, the abnormally proliferated membranous structures became labeled by the antibody. These results indicate that the rdgB protein is normally localized to the SRC membranes and probably adjacent photoreceptive membranes, suggesting the involvement of the rdgB protein in the phosphatidylinositol transfer between SRC and photoreceptive membranes.