Long-term dairy manure amendment promotes legacy phosphorus buildup and mobility in calcareous soils.

Continuous application of dairy manure to soils can lead to excessive phosphorus (P) accumulation (legacy P), which requires understanding for managing nutrient availability and leaching. This study was conducted in Kimberly, ID, where dairy manure or conventional fertilizer was applied to calcareous soil plots under continuous crop rotations for 8 years (2013-2020), followed by 2 years with no amendment. To understand legacy P behavior in the soils, total P, organic/inorganic P, and plant-available Olsen bicarbonate P and Truog extraction measurements were made from surface and subsurface samples. Additionally, P in soluble and less soluble calcium phosphate (Ca-P) minerals was estimated using selective extractions, and P desorption was measured in a flow-through reactor. Manure amendments resulted in increased total soil P and plant-available P, particularly in the initial 5 years. In the 0- to 30-cm depth, 54%-65% of the soil P added from manure amendments was readily soluble by the Truog P test. Phosphorus released from the 2022 manure-amended soil in the desorption experiments was about five times greater than the fertilizer-amended soil, suggesting high leaching potential. After 8 years of manure amendment, subsurface Olsen-P levels exceeded the 40 mg kg-1 management threshold, suggesting P adsorption potential of the surface had become saturated, allowing for P leaching. In the manure-amended surface soils, calcium phosphate minerals increased compared to the controls. Even after 2 years without manure amendment, soluble Ca-P mineral phases persisted in the soils, which can be a long-term source of P leaching.

The Role of the Trace Element Selenium in Inflammatory Bowel Disease.

One set of chronic gastrointestinal disorders called inflammatory bowel disease (IBD) is defined by persistent, non-specific inflammation. Abdominal pain, hematochezia, diarrhea, and other symptoms are among its clinical signs. Currently, managing and treating IBD remains a significant challenge. Patients with IBD frequently have deficits in trace elements. Selenium (Se) is one of the necessary trace elements for normal organismal function. It has several regulatory effects, including anti-oxidation, anti-inflammatory, and defensive properties, via inducing the synthesis of selenoproteins. Patients with IBD have been shown to have lower Se levels in epidemiologic research studies. Several experimental models of IBD suggest that Se or selenoproteins play a key role in microinflammation. We discuss the relationship between Se and IBD in this review, with an emphasis on a summary of potential mechanisms of action and applications of Se in IBD.

Obstetric and neonatal outcomes after programmed frozen embryo transfer with or without GnRH agonist for polycystic ovary syndrome: secondary analysis results from a randomized controlled trial.

BACKGROUND: Recent studies have reported higher risks of adverse maternal and neonatal outcomes, such as hypertensive disorders of pregnancy, after programmed frozen embryo transfer, especially in cycles with gonadotropin-releasing hormone agonist pretreatment. It remains unclear if GnRH agonist pretreatment before programmed frozen embryo transfer further increases the risk for obstetrical complications among women with polycystic ovary syndrome. OBJECTIVE: This study aimed to compare the obstetrical and neonatal complications of singleton and twin pregnancies after programmed frozen embryo transfer with or without gonadotropin-releasing hormone-a pretreatment among women with polycystic ovary syndrome. STUDY DESIGN: This was a secondary analysis of a single-center, randomized controlled trial comparing the live birth rate and cost-effectiveness of programmed cycle-prepared frozen embryo transfers with or without gonadotropin-releasing hormone agonist pretreatment among women with polycystic ovary syndrome. The analysis was restricted to women with polycystic ovary syndrome, aged 24 to 40 years, who delivered live neonates after frozen-thawed blastocyst transfer. The obstetrical and neonatal outcomes were compared between programmed cycles with and those without gonadotropin-releasing hormone agonist pretreatment. The adjusted odds ratios with 95% confidence intervals were calculated and adjustments were made for relevant confounders. RESULTS: The maternal and neonatal complications associated with 177 live single births and 38 twin births (253 newborns in total) were analyzed. There were no significant differences in the frequencies of obstetrical complications, including hypertensive disorders of pregnancy, between the gonadotropin-releasing hormone agonist pretreatment and no pretreatment group for both singleton and twin pregnancies. However, there was a significantly greater incidence of having a low birthweight neonate among singleton infants born after gonadotropin-releasing hormone agonist pretreatment when compared with no pretreatment (10.2% vs 1.3%; P=0.042), and a low birthweight among singleton infants was still more likely after adjusting for confounders (relative ratio, 3.85; 95% confidence interval, 1.13-7.11; P=.024). Other neonatal complications were all comparable between the pretreatment and no pretreatment groups for both singleton and twin pregnancies. CONCLUSION: For women with polycystic ovary syndrome, programmed frozen embryo transfer cycles with gonadotropin-releasing hormone agonist pretreatment could lead to a greater risk of having a low birthweight singleton neonate.

Low levels of arsenic and cadmium in rice grown in southern Florida Histosols - Impacts of water management and soil thickness.

Rice is planted as a rotation crop in the sugarcane-dominant Everglades Agricultural Area (EAA) in southern Florida. The Histosols in this area are unlike other mineral soils used to grow rice due to the high organic content and land subsidence caused by rapid oxidation of organic matter upon drainage. It remains unknown if such soils pose a risk of arsenic (As) or cadmium (Cd) mobilization and uptake into rice grain. Both As and Cd are carcinogenic trace elements of concern in rice, and it is important to understand their soil-plant transfer into rice, a staple food of global importance. Here, a mesocosm pot study was conducted using two thicknesses of local soil, deep (D, 50 cm) and shallow (S, 25 cm), under three water managements, conventional flooding (FL), low water table (LWT), and alternating wetting and drying (AWD). Rice was grown to maturity and plant levels of As and Cd were determined. Regardless of treatments, rice grown in these Florida Histolsols has very low Cd concentrations in polished grain (1.5-5.6 µg kg-1) and relatively low total As (35-150 µg kg-1) and inorganic As (35-87 µg kg-1) concentrations in polished grain, which are below regulatory limits. This may be due to the low soil As and Cd levels, high soil cation exchange capacity due to high soil organic matter content, and slightly alkaline soil pH. Grain As was significantly affected by water management (AWD < FL = LWT) and its interaction effect with soil thickness (AWD-D ≤ AWD-S ≤ FL-D = LWT-S = LWT-D ≤ FL-S), resulting in as much as 62 % difference among treatments. Grain Cd was significantly affected by water management (AWD > FL > LWT) without any soil thickness impact. In conclusion, even though water management has more of an impact on rice As and Cd than soil thickness, the low concentrations of As and Cd in rice pose little health risk for consumers.

A molecular marker of milk composition traits in NCAM2 gene of Chinese Holstein.

The aim of this study was to detect the novel copy number variation (CNV) locus of NCAM2 gene in Chinese Holstein, and to analyze the effect of the novel CNV locus in NCAM2 gene on milk composition traits. The novel CNV locus of NCAM2 gene in 310 Chinese Holstein was detected by real-time quantitative fluorescent PCR (qPCR) and association analysis was performed between the novel CNV locus in NCAM2 gene and milk composition traits in Chinese Holstein. There are three CNV types of NCAM2 gene in Chinese Holstein: gain (increased copy number), median (normal copy number) and loss (deleted copy number). Statistical analysis revealed that there was a significant association between CNV types and milk fat rate (p < 0.05). Moreover, we also discovered that the milk production and milk protein rate of gain type is higher than that of loss type, but that of mediate type is lower than that of loss type. However, in terms of somatic cell score, loss type is higher than that of gain type, but that of mediate type is lower than that of gain type. These observations suggested that gain type can be used as a candidate molecular genetic marker of milk fat rate.HighlightsThe CNVs of the NCAM2 gene were detected and validated in Chinese Holstein.The type of CNV was successfully implemented using qPCR.The statistical analysis indicated that the CNV of the NCAM2 gene are significantly associated with milk fat rate.

Rice Cd Levels in Cambodia Ranged 3 Orders of Magnitude due to Season and Soil Cd Levels.

Cadmium (Cd) is a toxic trace element that can be transported from soil into rice grain, posing health threats to rice consumers. Among the global studies on rice grain Cd, only one market survey reported grain Cd levels from Cambodia, an important rice-growing country in Southeast Asia. Here, we collected paired rice and soil samples in the wet and dry seasons from major rice-growing regions across five provinces in Cambodia and report the relationships between plant Cd and soil Cd parameters. Both DTPA-extractable and nitric acid digestible soil Cd are significant predictors for Cd levels in rice straw and grain. Rice grain Cd concentrations ranged 3 orders of magnitude from 0.002 to 1.066 mg kg-1 with the median and mean concentrations of 0.024 and 0.091 mg kg-1, respectively; these values have an upper range that is higher than previously reported. The highest grain Cd levels were found in rice grown in the dry season from two provinces located southeast of Phnom Penh along the Lower Mekong River, and their corresponding soil Cd levels were relatively higher than those collected during the wet season and around the Tonle Sap. While the source of higher Cd may be geogenic or due to anthropogenic activities, our data demonstrate that geographical and perhaps seasonal differences in grain Cd exist even within a small country that might not be reflected in market surveys.