Subject(s)
Crohn Disease/microbiology , Crohn Disease/therapy , Feces/microbiology , Food, Formulated , Parenteral Nutrition, Total , Female , Humans , MaleABSTRACT
A new apoptosis inducer, ammocidin, was isolated from the culture broth of Saccharothrix sp. AJ9571. Ammocidin induced apoptotic cell death in Ras-dependent Ba/F3-V12 cells with an IC50 of 66 ng/ml. No cell death was observed in IL-3-dependent Ba/F3-V12 cells at less than 100 microg/ml of ammocidin. Ammocidin significantly reduced the phosphorylation level of MAPK and S6K that mediate the anti-apoptotic function of Ras.
Subject(s)
Actinomycetales/metabolism , Apoptosis/drug effects , Hematopoietic Stem Cells/drug effects , Macrolides/isolation & purification , Macrolides/pharmacology , Actinomycetales/growth & development , Apoptosis/physiology , Cell Line , Humans , Macrolides/chemistry , Macrolides/metabolism , ras Proteins/metabolismABSTRACT
The structure of ammocidin, a new apoptosis inducer in Ras-dependent cells from Saccharothrix sp. AJ9571, was elucidated to be as shown in Fig. 1 by NMR and degradation studies. Ammocidin consists of a 20-membered macrolide ring and three deoxy sugars identified as 6-deoxy-L-glucose, D-digitoxose and D-olivomycose.
Subject(s)
Actinomycetales/metabolism , Apoptosis/drug effects , Macrolides/chemistry , Macrolides/pharmacology , Actinomycetales/growth & development , Hematopoietic Stem Cells/drug effects , Macrolides/metabolism , Magnetic Resonance Spectroscopy , Molecular Structure , Spectrophotometry, Infrared , Spectrophotometry, Ultraviolet , ras Proteins/metabolismSubject(s)
Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Benzoxazoles/chemistry , Benzoxazoles/pharmacology , Streptomyces/metabolism , Antineoplastic Agents/isolation & purification , Antineoplastic Agents/metabolism , Benzoxazoles/metabolism , Drug Screening Assays, Antitumor , Enzyme Inhibitors/chemistry , Enzyme Inhibitors/isolation & purification , Enzyme Inhibitors/metabolism , Enzyme Inhibitors/pharmacology , Fermentation , Humans , Inhibitory Concentration 50 , Magnetic Resonance Spectroscopy , Molecular Structure , Streptomyces/classification , Topoisomerase II InhibitorsABSTRACT
Strain TP-AO121 which produces a complex of novel tyrosine kinase inhibitors designated hibarimicins A, B, C, D and G was considered to be a new subspecies of Microbispora rosea, and the name, Microbispora rosea subsp. hibaria, was proposed. Hibarimicins A, B, C and D specifically inhibited the src tyrosine kinase activity without affecting protein kinase A or protein kinase C. They also showed in vitro anti-Gram-positive bacterial and antitumor activities. The molecular formulae of hibarimicins A, B, C, D and G were assigned to be C85H112O37, C85H112O37, C83H110O36, C85H112O38, and C85H112O39 respectively.
Subject(s)
Actinomycetales/chemistry , Anti-Bacterial Agents/pharmacology , Signal Transduction/drug effects , Actinomycetales/classification , Actinomycetales/genetics , Animals , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/isolation & purification , Antibiotics, Antineoplastic/chemistry , Antibiotics, Antineoplastic/isolation & purification , Antibiotics, Antineoplastic/pharmacology , Chemical Phenomena , Chemistry, Physical , DNA, Fungal/genetics , Enzyme Inhibitors/chemistry , Enzyme Inhibitors/isolation & purification , Enzyme Inhibitors/pharmacology , Fermentation , Mice , Nucleic Acid Hybridization , Protein Kinase Inhibitors , Tumor Cells, Cultured , src-Family Kinases/antagonists & inhibitorsABSTRACT
The structure of hibarimicins A, B, C, D and G which are inhibitors for tyrosine specific protein kinase are determined using spectroscopic techniques. Hibarimicins described in this report consist of a common aglycon and six deoxyhexoses. The aglycon contains a highly oxidized naphtylnaphthoquinone as a chromophore. Among them, hibarimicin B was identical with angelmicin B.
Subject(s)
Actinomycetales/chemistry , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Signal Transduction/drug effects , Carbohydrates/chemistry , Enzyme Inhibitors/chemistry , Enzyme Inhibitors/pharmacology , Magnetic Resonance Spectroscopy , Molecular Structure , Protein-Tyrosine Kinases/antagonists & inhibitors , Spectrometry, Mass, Fast Atom BombardmentABSTRACT
Glucosylquestiomycin, a novel N-glucopyranoside of questiomycin A, was isolated from the culture broth of Microbispora sp. TP-A0184. The absolute configuration of the sugar was determined as D-configuration by chemical synthesis. The new antibiotic showed antibacterial activity against gram-positive and -negative bacteria and yeasts and cytotoxic activity against U937 cells.
Subject(s)
Anti-Bacterial Agents/isolation & purification , Anti-Bacterial Agents/chemical synthesis , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Antibiotics, Antineoplastic/chemical synthesis , Antibiotics, Antineoplastic/chemistry , Antibiotics, Antineoplastic/isolation & purification , Antibiotics, Antineoplastic/pharmacology , Fermentation , Glucosides/chemical synthesis , Glucosides/chemistry , Glucosides/isolation & purification , Glucosides/pharmacology , Gram-Negative Bacteria/drug effects , Gram-Positive Bacteria/drug effects , Humans , Inhibitory Concentration 50 , Magnetic Resonance Spectroscopy , Microbial Sensitivity Tests , Molecular Structure , Oxazines/chemical synthesis , Oxazines/chemistry , Oxazines/isolation & purification , Oxazines/pharmacology , Stereoisomerism , U937 Cells , Yeasts/drug effectsABSTRACT
We present a case of asymptomatic cor triatrium in a 78-year-old man, in whom the condition was diagnosed and the severity of the lesion was accurately assessed by biplanar transesophageal echocardiography. To our knowledge, our patient is the oldest living person with cor triatrium.
Subject(s)
Cor Triatriatum/diagnostic imaging , Aged , Echocardiography, Doppler, Color , Echocardiography, Doppler, Pulsed , Echocardiography, Transesophageal , Humans , MaleABSTRACT
To determine the relationship between airway and coronary artery spasmogenesity in patients with vasospastic angina pectoris (VSAP), we measured the bronchial responsiveness to inhaled methacholine in 21 VSAP patients (V-group) and compared it to that of 17 chest pain syndrome patients with normal coronary (N-group). In the V-group, logarithmic values of the minimum cumulative dose required to decrease respiratory conductance from the baseline (Log Dmin) was significantly lower than that in N-group (0.47 +/- 0.60 log units vs. 0.98 +/- 0.60 log units respectively, p < 0.05). Log Dmin was significantly correlated with the dose of acetylcholine which induced a greater than 90% stenosis of the coronary artery (r = 0.383, p < 0.05). We concluded that both the coronary artery and the airway show hyperresponsiveness to cholinergic agonists in patients with VSAP.
Subject(s)
Angina Pectoris/physiopathology , Bronchial Hyperreactivity/physiopathology , Coronary Vasospasm/physiopathology , Aged , Bronchial Provocation Tests , Female , Humans , Male , Methacholine Chloride , Middle AgedSubject(s)
Dental Bonding , Dental Soldering , Denture Precision Attachment , Adhesives , Dental Abutments , HumansSubject(s)
Brain/diagnostic imaging , Tomography, X-Ray Computed , Adult , Brain/anatomy & histology , Cephalometry , Humans , Middle AgedABSTRACT
Review was made on the effect of adenosine triphosphate, 1.0-3.0 mg/kg intravenously, on the cerebral circulation and the presence of the dilating action on the cerebral blood-vessels in cats. 1. The group of the normal intra-cranial pressure showed, as reported previously, the transient hypotension and its complicated decrease of cerebral blood-circulation at the initial period for 30-100 check, then demonstrating the increase of cerebral blood-flow for 200-1000 check. Its increased volume and duration were dose-dependent. 2. The group of the loaded intracranial pressure of 20-30 mmHg (slightly elevated group) showed the increase of cerebral blood-flow, like the group of the normal intracranial pressure. However, the effect of ATP showed the decrease or disappearance in accordance with the elevation of the loaded pressure. 3. Cerebral vasodilating effect of ATP was promptly observed after the administration, and its degree was 30-50% in photographical observation. 4. Increasing effect on cerebral blood-flow and cerebral vasodilating action of ATP were similar in the pattern to that of vasodilators such as papaverine hydrochloride.
Subject(s)
Adenosine Triphosphate/pharmacology , Cerebral Arteries/drug effects , Cerebrovascular Circulation/drug effects , Animals , Basilar Artery/drug effects , Blood Volume/drug effects , Cats , Intracranial Pressure/drug effects , Vasomotor System/drug effectsABSTRACT
Raman spectrum of purified formylmethionine transfer RNA from Escherichia coli has been observed in its aqueous solution. In the 1800 to 200 cm(-l) range, about 30 Raman lines are found, each of which can be assigned to one of the constituent nucleotide residues. From the positions and intensities of the lines, information on the intramolecular environments of these base residues can be obtained.