1.
Analyst
; 125(4): 569-73, 2000 Apr.
Article
in English
| MEDLINE
| ID: mdl-10892013
ABSTRACT
The isolation and identification is described of MHC class II-bound peptides derived from Langerhans cells. A combination of preparative micro-HPLC, MALDI-MS, Edman degradation was used for determining the amino acid sequence of MHC-associated peptides. Sample handling was crucial because fractions containing trace amounts of material require immediate storage at -80 degrees C to prevent peptide losses.