[REPARATIVE ACTIVITY OF ORIGINAL SUBSTANCE BASED ON ALGINIC ACID].

The influence of an original substance based on enzymatically destructed alginic acid on the reparative activity has been studied using an experimental model of planar aseptic wound. Toxicity of the test compound was evaluated and its effective dose was determined using curiozin as the reference drug. It is found that the test compounds is non-toxic. It is established that the pharmacological activity of the reference drug (curiozin) is lower on the average by 3.5% (δ = 0.021) than that of the original pharmaceutical substance based on destructed alginic acid

[Koletex-M absorbent paper use in acute festering odontogenic periostitis treatment].

150 patients with acute festering odontogenic periostitis were under observation. In 75 of them Koletex-M absorbent paper was used as draining material containing as active components metronidazole, dimexide and sodium alginate. In the control group of patients traditional schemes of treatment were used. Treatment process efficacy was estimated by microbiological and cytological studies. It was found that Koletex-M absorbent paper use reduced postoperative wound microbe semination. In 3 days the cells of regenerative series were found that led to speedy wound healing and reduction of temporary disability terms.

Complex N-acetyl-L-cysteine compounds with biometals as self-defense factors of biological system.

We studied the effects of complex N-acetyl-L-cysteine compounds with transitional biometals on the inflammatory and adaptation reactions. Some compounds were superior to known antihypoxants and actoprotectors and exhibited significant antiinflammatory activity.

[Conversion of L-cystine and L-cysteine to taurin by the enzyme systems of liver cells]. / Transformatsiia L-tsistina i L-tsisteina v taurin pod deistviem fermentnykh sistem kletok pecheni.

The kinetics of conversion of sulfur-containing amino acids L-cystine and L-cysteine to taurin by the enzyme system of cattle liver cells was studied, and a mathematical model was developed. It was shown that L-cystine and L-cysteine conversion obeyed the Michaelis-Menten equations of serial-sequential conversions with regard to inhibition by the final product and inactivation. The yield of taurin under the optimized conditions of L-cystine and L-cysteine conversion (temperature, 40 degrees C; pH 1.5 and 3.0, respectively; and addition of enzyme preparations in five equal portions at 2-h intervals) was in the range 80-85% of the substrate weight.

[Gastroprotective effect of lycopene in acute gastritis induced by hydrocortisone]. / Issledovanie gastroprotektivnoi aktivnosti likopina na modeli ostrogo gastrita, indutsirovannogo gidrokortizonom.

Lycopine in a daily dose of 0.2 mg/kg produced a gastroprotector effect on the model of hydrocortisone-induced gastritis in rats. A change in the dosage (toward significant increase or decrease) significantly reduces the gastroprotector effect.

[Preventive effect of carotene-containing preparations in acute gastritis model]. / [Preventivnoe deistvie karotinsoderzhashchikh preparatov na modeli ostrogo gastrita]

A preliminary course of treatment with cascatol and beta-clamin, original drugs of natural origin, prevented the butadion damage of mucous membrane of the stomach in experimental rats. An analysis of the morphometric data of histologic samples taken from the test animals showed the effect to be dose-dependent. A 40-day treatment with cascatol and beta-clamin in a single daily dose of 0.05 mg/kg produced a gastroprotector effect, while the same drugs in a dose of 0.5 mg/kg offered no protection for the stomach against the butadion damage.

[Combined effect of visible light sensitized by chlorine e6 and streptomycin on the obligate pathogenic bacterium Klebsiella rhinoscleromatis]. / Kombinirovannoe sensibilizirovannoe khlorinom e6 deistvie vidimogo sveta i streptomitsina na obligatno-patogennye bakterii Klebsiella rhinoscleromatis.

Chlorine e6-sensitized photodamage of Klebsiella rhinoscleromatis (an obligate pathogen) in the presence of streptomycin was studied. The pathogen is highly resistant to photosensitization characteristic of other gram-negative bacteria. Marked synergism of the bactericidal effect of the chlorine e6-sensitized visible light and streptomycin on K. rhinoscleromatis was observed. It was suggested that the synergistic action was due to the oxidative damage of the cell membranes resulting in higher rates of the streptomycin penetration to the cells and impairment of the intracellular protective mechanisms at the molecular level. The findings are in favour of the further investigation aimed at the development of a comprehensive method of photo- and chemotherapy of scleroma.

[The antistaphylococcal properties of plant extracts in relation to their prospective use as therapeutic and prophylactic formulations for the skin]. / Antistafilokokkovye svoistva nekotorykh ékstraktov rastenii v sviazi s perspektivoi ispol'zovaniia v lechebno-profilakticheskikh sostavakh dlia kozhi.

Antistaphylococcal activities of plant extracts (12 water alcohol glycerol, WAG, 6 water alcohol, WA, 8 alcohol glycerol, AG, extracts) towards reference strains and those isolated from patients with pyoinflammatory diseases of the skin were examined by diluting the preparations in solid media. The strains under study were 69 S. aureus, 44 S. epidermidis, and 2 S. saprophyticus ones. Fifteen plant extracts have shown antistaphylococcal activities. The most active were oak bark, sage and St. John's wort grass WAG extracts, horse radish root and leaf AG extracts, celandine grass WA extract; bur marigold and yarrow grass WA extracts were active towards S. aureus. S. aureus strains isolated from patients were found less sensitive to oak bark, German camomile flower WAG and celandine, bur marigold, and brewing waste WA extracts that the reference strains. S. epidermidis strains isolated from patients with acne rash were less sensitive to sweet flag rhizome WAG, celandine and brewing waste WA extracts that the reference strains. These data may be useful when developing compositions including plant extracts for patients with skin diseases.

[The intensity and dynamics of the development of immunity in the administration of experimental corpuscular ozena vaccines]. / Napriazhennost' i dinamika razvitiia immuniteta pri vvedenii éksperimental'nykh ozenoznykh korpuskuliarnykh vaktsin.

Experiments on the active protection of mice from ozenous infection in its two forms, generalized (acute sepsis) and local (plantar infiltration), have demonstrated that immunity, induced by experimental heat-killed ozena vaccine (Klebsiella ozaenae strain 2211, antigens 02B:K4) introduced in a single injection, is characterized by sufficiently high intensity (the degree of protection increases up to 10,000-fold) and duration (at least 30 days). In both forms the development of immunity is characterized by a rapid rise of its intensity to the maximum level (achieved by the end of week 1), subsequent decrease by weeks 3-4 and disappearance by days 50-60 after immunization. Immunity becomes more intense with the increase of the number of injections if these injections are separated by sufficient intervals (up to 14 days). The optimum schedule used in the study of postvaccinal immunity to experimental generalized and local ozenous infection consists of the subcutaneous injection of K. ozaenae strain 2211 in a dose of 250-500 million microbial bodies per mouse with the subsequent challenge with the virulent strain on week 2 from the date of immunization.

[Interrelations between Klebsiella ozaenae and other members of a microbial association in a pathological ozenous focus]. / Vzaimootnosheniia mezhdu Klebsiella ozaenae i drugimi chlenami mikrobnoi assotsiatsii patologicheskogo ozenoznogo ochaga.

The study of relationships between individual representatives of the microeubiosis at a pathological ozenous focus has shown that Gram-positive microorganisms and opportunistic enterobacteria are not antagonistic to K. ozaenae which, in their turn, do not affect the growth of opportunistic enterobacteria and Pseudomonas aeruginosa. The overwhelming majority of P. aeruginosa strains (92.9%) grown on a solid culture medium are characterized by a varying antagonistic activity with respect to K. ozaenae; this activity does not manifest if these organisms have been grown together in a liquid medium. P. aeruginosa culture fluid diluted 1:10 and 1:20 has not inhibited the multiplication of K. ozaenae. The data indicate the possibility of a prolonged coexistence of K. ozaenae with Proteus and P. aeruginosa, which is confirmed by many cases of simultaneous isolation of associations of these microbes from ozena patients. Thus the possibility appears of a combined pathogenic action of these bacteria on human body.

[Immunogenicity of inactivated Klebsiella ozaenae cultures in relation to the properties and methods of culturing and preserving the initial strains]. / Immunogennost' inaktivirovannykh kul'tur Klebsiella ozaenae v zavisimosti ot svoistv, metodov kul'tivirovaniia i khraneniia iskhodnykh shtammov.

Experiments in the active protection of mice from generalized K. ozaenae infection have demonstrated that the heat-killed cultures of K. ozaenae capsular strains (antigens 02B : K4) possess pronounced and stable immunogenic properties, dependent on the presence and type of the capsular antigen and independent of the virulence and age of the initial strain, as well as the time and methods of its cultivation (the type of the culture medium: nutrient agar, glucose-mineral medium) and storage (the term of observation is 2 years). This investigation has resulted in the determination of the strain (2211) with the highest and most stable protective properties and in the selection of the optimum conditions for the immunization of mice (by the subcutaneous injection of 250 microbial bodies per mouse) with its heat-killed culture.

[Comparative study of the resistance of hospital and nonhospital strains of Pseudomonas aeruginosa to antibacterial preparations]. / Sravnitel'noe izuchenie ustoichivosti k antibakterial'nym preparatam gospital'nykh i vnegospital'nykh shtammov Pseudomonas aeruginosa.

The comparative study on 392 hospital and 160 out-of-hospital Pseudomonas aeruginosa strains showed that the hospital strains differed in the levels and spectra of resistance to antibiotics, bacteriostatic and bactericidal concentrations of antiseptics and disinfectants, the resistancevars composition, serogroups and biochemical activity. A scheme for dividing Ps. aeruginosa into resistancevars according to the antibiotic resistance spectra is presented. The hospital strains in this scheme belonged mainly to the variants with a resistance to 3--5 drugs and the out-of-hospital strains belonged to the variants resistant to 1--2 drugs. The biological characteristics of the hospital strains made them more advantageous as compared to the out-of-hospital strains when existing in the hospital ecosystem. The changeability of Pseudomonas aeruginosa is considered to be geographically related (group, interpopulational). The hospital strains of this species were classified as belonging to a higher taxon, i. e. ecological types or variants.

[Role of immune and normal sera in protection from ozena]. / Rol' immunnykh i normal'nykh syvorotok v zashchite ot ozenoznoi infektsii.

For the first time high preventive properties of sera obtained from ozena patients and healthy persons have been established on the model of acute ozena sepsis. The preventive properties of sera obtained from ozena patients and containing antibodies to the somatic antigens of the causative agent of ozena (O-antibodies) are higher than those of sera obtained from healthy persons. But this effect is linked not with O-antibodies, but probably with the presence of antibodies to the capsular antigen of the microbe (C-antibodies), which are not detected in the agglutination test. The indirect proof of the presence of C-antibodies in the sera of ozena patients, containing O-antibodies, have been obtained due to the ability of C-antibodies to enhance in vivo and to decrease in vitro the bactericidal activity of sera obtained from such patients.