Subject(s)
Chondrodysplasia Punctata , Intracranial Hemorrhages , Humans , Female , Pregnancy , Chondrodysplasia Punctata/genetics , Chondrodysplasia Punctata/diagnostic imaging , Intracranial Hemorrhages/diagnostic imaging , Intracranial Hemorrhages/embryology , Intracranial Hemorrhages/etiology , Ultrasonography, Prenatal , Fetal Diseases/diagnostic imaging , Fetal Diseases/genetics , Infant, Newborn , AdultABSTRACT
Using a calpain/protein kinase C (PKC) complex, we were able to reproduce, in vitro, the induction of PKC down-regulation by the phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate (TPA) which had been previously observed in cells. We show that TPA initiates this phenomenon by promoting a calpain-dependent conversion of PKC to the Ca2+ phospholipid-independent protein kinase M (PKM), at physiological calcium concentrations. This effect of TPA was dependent upon the presence of phosphatidylserine and was observed only when PKC was the substrate for the protease, inactivation of calpain by autolysis not being modified by the presence of TPA. Moreover, PKM generated from the calpain-PKC complex was resistant to calpain, even after addition of TPA. These results suggest that TPA induces a conformational change in PKC, increasing the affinity of the kinase for calpain and consequently permitting its proteolysis for the basal level of calcium in cells.
Subject(s)
Calpain/analysis , Protein Kinase C/analysis , Tetradecanoylphorbol Acetate/pharmacology , Animals , Calcium/pharmacology , Dose-Response Relationship, Drug , Down-Regulation , In Vitro Techniques , Male , Protein Kinase C/metabolism , RabbitsABSTRACT
A calpain 1-protein kinase C (PKC) complex was isolated from rabbit skeletal muscle by hydrophobic interaction chromatography on phenyl-sepharose and by strong anion exchange chromatography on Q-Sepharose. Calpain 1 and kinase activities were then dissociated on a phenyl-Sepharose matrix using gradients of decreasing ionic strength. The purified PKC obtained corresponded to conventional PKC and was recognized by a monoclonal antibody specific for alpha and beta isotypes. Leupeptin, calpain inhibitor II, and the more selective calpain inhibitors calpeptin and MDL 28170 did not block the activation of the purified PKC by Ca2+ and phosphatidylserine.