ABSTRACT
Fanconi anemia is primarily inherited as an autosomal recessive genetic disorder with common delays in diagnosis and challenging treatments. Fanconi anemia patients have a high risk of developing solid tumors, particularly in the head and neck or anogenital regions. The diagnosis of Fanconi anemia is primarily based on the chromosomal breakage but FA gene sequencing is recommended in all patients with a positive chromosome fragility test. Here, we present a 32-year-old man with advanced tonsil squamous cell carcinoma and fatal toxicity after the first cycle of chemotherapy. No anemia was present. A recent variant mutation if the FANCM gene was detected (c1511_1515delGAGTA (pArg504AsnfsTer29)). Homozygous or double heterozygous pathogenic variants have been reported in FANCM and linked to azoospermia and primary ovarian failure without anemia. Alterations in this gene have also been associated with a genetic predisposition for solid tumors (breast and ovarian cancer) and hematological malignancies (B-cell acute lymphoblastic leukemia). Due to the hypersensitivity of these patients to DNA-damaging agents such as chemotherapy and radiotherapy, surgery is the best treatment option for malignant solid tumors. Dose reductions or alternative regimens of chemotherapy and/or radiotherapy are recommended in FA patients who develop a malignant tumor.
Subject(s)
Antineoplastic Agents/adverse effects , Carcinoma, Squamous Cell , Cisplatin/adverse effects , DNA Helicases/genetics , Fanconi Anemia/genetics , Tonsillar Neoplasms , Adult , Carcinoma, Squamous Cell/drug therapy , Carcinoma, Squamous Cell/genetics , Carcinoma, Squamous Cell/radiotherapy , Fatal Outcome , Humans , Male , Mutation , Tonsillar Neoplasms/drug therapy , Tonsillar Neoplasms/genetics , Tonsillar Neoplasms/radiotherapyABSTRACT
The high incidence of norovirus (NoV) infections seems to be related to the emergence of new variants that evolved by genetic drift of the capsid gene. In this work, that represents a first effort to describe the molecular epidemiology of NoV in the northwest of Spain, a total of eight different NoV genotypes (GII.1, GII.3, GII.4, GII.6, GII.7, GII.12, GII.13, GII.14) were detected. The major genotypes observed were GII.4 (45·42%) and GII.14 (34·9%), being detected in all age groups. In addition, and although most of GII.4 sequences belonged to 2006b (7·2%) and 2010 (50·35%) variants, the presence of new NoV variants was observed. Phylogenetic analysis revealed that a high number of GII.4 sequences (35·24%) could be assigned to the newly emerging Sydney 2012 variant, even during late 2010. The high prevalence of NoV GII.14 observed in this study may indicate the emergence of this genotype in Spain.
Subject(s)
Caliciviridae Infections/epidemiology , Caliciviridae Infections/virology , Gastroenteritis/epidemiology , Gastroenteritis/virology , Norovirus/genetics , Adolescent , Adult , Child , Child, Preschool , Feces/microbiology , Genotyping Techniques , Humans , Infant , Infant, Newborn , Middle Aged , Molecular Epidemiology , Norovirus/classification , Norovirus/isolation & purification , Phylogeny , Prevalence , Spain/epidemiology , Young AdultABSTRACT
Hepatitis A virus (HAV) represents a significant public health problem due to its high persistence in the environment and its transmission through contaminated water and food. Bivalve shellfish are filter feeders that can bioaccumulate human pathogens found in contaminated waters, their consumption being a potential cause of hepatitis A outbreaks. In this work, cultured and wild bivalve shellfish from the Ría de Vigo (Galicia, NW Spain) were analysed for the presence and genotyping of HAV. A total of 160 shellfish samples were collected between March 2004 and December 2006, including 68 samples from cultured mussels (Mytilus galloprovincialis), 30 from wild clams (Rupitapes decussatus), 31 from wild cockles (Cerastoderma edule) and 31 from wild mussel. HAV detection, carried out by quantitative RT-PCR, was positive for 29 (42.6%) cultured and 40 (43.5%) wild samples, with levels ranging from 3.1 x 10(2) and 1.4 x 10(10) RNA copies/g of shellfish digestive tissue. The phylogenetic analysis of VP1-P2A and VP3-VP1 regions, separately or as concatenated sequences, revealed that all HAV strains analysed belong to subgenotype IB. These results indicate a high prevalence of this subgenotype in the area studied.
Subject(s)
Hepatitis A virus/genetics , Shellfish/virology , Animals , Bivalvia/virology , Fresh Water/virology , Genotype , Hepatitis A/epidemiology , Hepatitis A virus/classification , Hepatitis A virus/isolation & purification , Humans , Phylogeny , RNA, Viral/genetics , RNA, Viral/isolation & purification , SpainABSTRACT
The main defense mechanisms of the human body are described. The essential steps of the immune responses are summarized, emphasising the importance of cytokines, especially II-1, TNF and TGF. Alcohol acts as an immunosuppressor in different ways, namely through suppression of TNF synthesis. The consequence is the appearance of respiratory infections, including pneumonia, tuberculosis, AIDS, infections and cancer of the digestive tract and a status of chronic inflammation which leads to hepatic cyrrhosis.
Subject(s)
Ethanol/adverse effects , Infections/immunology , HumansABSTRACT
The authors present a case report of a pseudotumor of the stomach and a brief discussion about this very unusual entity. A 75-year-old female patient was admitted with melena and a large epigastric tumor; she underwent upper gastrointestinal endoscopy, abdominal ultrasound, magnetic resonance imaging, guided needle aspiration and angiography. Preoperative diagnostic hypothesis included a partially thrombosed aneurysm of the splenic artery, pancreatic cystic neoplasm with gastric invasion and pancreatic pseudocyst complicated with hemorrhage. Laparotomy revealed a gastric tumor and the patient was submitted to a radical subtotal Billroth II gastrectomy. Only the pathologic examination revealed the unexpected definitive diagnosis of an organized intramural gastric hematoma. There were no postoperative complications and she remains asymptomatic 10 months after surgery.
Subject(s)
Stomach Neoplasms/diagnosis , Aged , Aneurysm/diagnosis , Diagnosis, Differential , Female , Gastrectomy/methods , Hematoma/diagnosis , Humans , Pancreatic Neoplasms/diagnosis , Splenic Diseases/diagnosis , Stomach Diseases/diagnosis , Stomach Neoplasms/pathology , Stomach Neoplasms/surgeryABSTRACT
The relative activities of some hydrogen-donating antioxidants were assessed by comparing their activities with that of Trolox (Trolox equivalent antioxidant capacity, TEAC) for scavenging the ABTS radical cation (ABTS.+) generated in the aqueous phase. We have verified, however, that TEAC values may change with the concentration of compounds and with the measuring times used. Not withstanding, TEAC values do not differ significantly if the compounds have kinetic curves of ABTS.+ formation similar to that of Trolox. This is the case with ascorbic acid, whose TEAC values, determined by using five concentrations at three different measuring times, are very close. For the flavonoids studied (catechin, rutin, naringenin and silibinin) which have kinetic curves of ABTS.+ formation different from that of Trolox, the TEAC values decrease with increasing concentrations of the compounds for each measuring time, and increase with increasing measuring times for each concentration. In the present study, we conclude that, in order to evaluate relative antioxidant activities of compounds by the ABTS assay, it is essential to perform kinetic studies to assess scavenging of ABTS.+ by these compounds. Therefore, when the TEAC values of compounds are determined for more than one measuring time, we may be sure that all the antioxidant potential of compounds is being considered and whether or not it is possible to establish a hierarchy for their antioxidant activities.
Subject(s)
Antioxidants/pharmacology , KineticsABSTRACT
Oxidative stress is implicated in the pathogenesis of various cardiovascular disorders. The knowledge, characterisation and comparison of the different antioxidant properties of some cardiovascular drugs might lead to new therapeutic approaches. Blood constituents are biological products easier to obtain. Their oxidative damage is accepted to be involved in several pathogenic pathways such as atherogenesis. Blood products can be utilised as in vitro models of macromolecular and cellular oxidative damage. It is well known that transition metals catalyse the generation of more damaging reactive oxygen species. We studied the antioxidant effect of four beta-adrenergic blocking agents (pindolol, propranolol, atenolol and metoprolol) and of one calcium channel antagonist (nifedipine), on the plasma oxidative damage induced by copper. Lipid peroxidation was evaluated by measuring fluorescent substances. We added the drugs to the assay system, before and after the induction of peroxidation by copper, to elucidate their ability to prevent and/or block lipid peroxidation. We observed that pindolol, propranolol and nifedipine have antioxidant properties, in the assay system, when added 15 min before the addition of copper. This effect is demonstrated by the delay in initiation and decrease in formation of lipid peroxidation products. Nifedipine also has a remarkable chain breaking effect. We compared the drugs with ascorbic acid in their relative antioxidant effect: nifedipine > ascorbate > pindolol > propranolol.
Subject(s)
Adrenergic beta-Antagonists/pharmacology , Antioxidants/pharmacology , Calcium Channel Blockers/pharmacology , Nifedipine/pharmacology , Ascorbic Acid/pharmacology , Atenolol/pharmacology , Copper/pharmacology , Female , Fluorescence , Humans , Lipid Peroxidation , Male , Metoprolol/pharmacology , Pindolol/pharmacology , Propranolol/pharmacologySubject(s)
AIDS-Related Opportunistic Infections , Pericarditis, Tuberculous , AIDS-Related Opportunistic Infections/diagnosis , AIDS-Related Opportunistic Infections/drug therapy , AIDS-Related Opportunistic Infections/immunology , Adult , Antitubercular Agents/therapeutic use , CD4 Lymphocyte Count , Female , Humans , Isoniazid/therapeutic use , Pericarditis, Tuberculous/diagnosis , Pericarditis, Tuberculous/drug therapy , Pericarditis, Tuberculous/immunologyABSTRACT
The general adaptation syndrome is discussed on the light of recent discoveries on hypothalamic peptides and of their possible influence in survival and in induction of diseases. The problem of stress in alcoholism is reviewed. The author ends with a short souvenir of Hans Selye.
Subject(s)
Stress, Psychological/physiopathology , Alcoholism/physiopathology , Animals , General Adaptation Syndrome/physiopathology , Glucocorticoids/physiology , Humans , Neuropeptides/physiologyABSTRACT
A 65-year-old man with primary haemochromatosis was admitted because of fever and confusion. He was found to have bacteraemia and meningitis due to Listeria monocytogenes. Treatment with ampicillin plus tobramycin was instituted, and despite an initial improvement, the patient experienced an unfavourable course and died. At postmortem examination, tricuspid valve endocarditis and purulent pericarditis with tamponade were detected. Listeria monocytogenes grew in the culture of the pericardial fluid. Documentation of Listeria monocytogenes pericarditis is extremely rare, and data on the patient described and on seven published cases are reported.
Subject(s)
Endocarditis, Bacterial/complications , Hemochromatosis/complications , Listeriosis/complications , Meningitis, Listeria/complications , Pericarditis/complications , Aged , Bacteremia/complications , Endocarditis, Bacterial/microbiology , Fatal Outcome , Female , Humans , Listeria monocytogenes/isolation & purification , Listeriosis/microbiology , Male , Pericarditis/microbiologyABSTRACT
Silibinin (SDH) is a flavonoid with ascertained hepatoprotective effects, which have been partially attributed to its antioxidant properties. Oxidation of blood constituents could have a role in atherogenesis and interfere with the rheologic properties of the blood. In this study we investigated, whether SDH could protect some blood constituents against oxidative modification. In human plasma we measured TBARS and fluorescence generation as indicators of copper or azobis amidinopropane hydrochloride (AAPH) at 760 mm Hg PO2-induced lipid peroxidation. SDH at 50 microM inhibited copper-induced TBARS formation by 25% and fluorescence by 47%. SDH also inhibited AAPH-induced lipid peroxidation, but at 175 microM concentration only. Oxidative modification of albumine was evaluated by fluorescence generation. SDH at 50 microM inhibited copper/hydrogen peroxide fluorescence generation by 54% and at 2.5 microM it inhibited EDTA-Fe (II)/hydrogen peroxide fluorescence generation by 31%. The protection of albumin by SDH was confirmed by SDS-PAGE electrophoresis. Copper-induced red-cell lipid peroxidation was evaluated by TBARS formation. SDH at 250 microM inhibited copper-induced lipid peroxidation and hemolysis by 45% and 94%, respectively. SDH also inhibited hemolysis in red-cell suspensions exposed to hydrogen peroxide, but not lipid peroxidation. Our results show that SDH may protect blood constituents from oxidative damage.
Subject(s)
Blood/metabolism , Oxidation-Reduction/drug effects , Plasma Substitutes/metabolism , Silymarin/pharmacology , Erythrocytes/metabolism , Hemolysis/drug effects , Humans , Lipid Peroxidation/drug effects , Serum Albumin/metabolismABSTRACT
Ethanol is a powerful generator of oxygen free radicals, when metabolized in the liver or in other organs. Isoenzyme 2E1 of cytochrome P450 and aldehyde oxidase are the main mechanisms for the generation of these radicals. A consequence of free radical generation is a decrease in protein synthesis. As a result we have endocrine and immunity alterations. The paper ords with a brief discussion of stress associated to alcoholism.
Subject(s)
Ethanol/metabolism , Stress, Physiological/metabolism , Alcoholism/immunology , Animals , Endocrine System Diseases/metabolism , Free Radicals , Humans , Protein Biosynthesis , Stress, Physiological/immunologySubject(s)
Adenocarcinoma/complications , Bordetella Infections/etiology , Bordetella bronchiseptica/isolation & purification , Opportunistic Infections/etiology , Pneumonia, Bacterial/etiology , Prostatic Neoplasms/complications , Adenocarcinoma/immunology , Aged , Bordetella Infections/microbiology , Fatal Outcome , Humans , Immunocompromised Host , Intestinal Obstruction/complications , Intestinal Obstruction/microbiology , Intestinal Obstruction/surgery , Male , Opportunistic Infections/microbiology , Pneumonia, Bacterial/microbiology , Prostatic Neoplasms/immunologyABSTRACT
Diamine oxidase (DAO) or histaminase is an enzyme which deaminates histamine and several aliphatic amines to their corresponding aldehydes. Hydrogen peroxide and ammonia are side products of this reaction. The purpose of the present work was to evaluate if determination of produced hydrogen peroxide reflects DAO activity or if intermediate formation of the superoxide radical could be a reason for lack of correspondence between oxygen uptake and hydrogen peroxide production at different pH. Superoxide radical formation was determined by cytochrome c reduction in the presence and absence of superoxide dismutase (SOD). Oxygen uptake was measured with an oxygen electrode and hydrogen peroxide production by a spectrophotometric method. At pH 6.6 there was no superoxide production, but at pH 7.4 there was some, and it increased markedly at pH 9.5. Oxygen uptake also increased with increasing pH, especially with histamine as substrate. These results lead us to suggest that the mechanism of action of DAO involves the intermediate generation of superoxide radicals.
Subject(s)
Amine Oxidase (Copper-Containing)/metabolism , Amines/metabolism , Superoxides/metabolism , Animals , Benzylamines/metabolism , Cadaverine/metabolism , Colorimetry , Histamine/metabolism , Hydrogen Peroxide/analysis , Hydrogen-Ion Concentration , Kidney/enzymology , Kinetics , Manometry , Oxidation-Reduction , Putrescine/metabolism , Substrate Specificity , SwineABSTRACT
BACKGROUND: The glycosaminoglycans metabolism is disturbed in progressive systemic sclerosis (PSS). Serum hyaluronic acid (HA) is elevated in this disease. OBJECTIVE: This study was conducted to determine the HA plasma concentrations of patients with PSS according to the different stages of the disease. METHODS: We studied 48 patients divided into three subgroups: subgroup 1 (n = 10), with skin compromise without evidence of other organ involvement; subgroup 2 (n = 21), with skin and esophagus involvement; subgroup 3 (n = 17), with skin, lung and other internal organ involvement. A radiometric assay was performed for quantification of HA. RESULTS: Our results confirm the increase in plasma HA in patients with PSS. They also suggest that lung involvement is the main feature responsible for high plasma concentrations of HA. The plasma HA levels were elevated in patients compared to normals (p <0.001). Significant differences were observed between subgroups 1 and 3 (p <0.01) and between subgroups 2 and 3 (p <0.01). A positive correlation between disease severity scores and plasma HA values was observed (p <0.01). CONCLUSION: An important elevation of HA plasma levels could be a serologic marker of disease severity, progression and degree of visceral involvement.
Subject(s)
Hyaluronic Acid/blood , Scleroderma, Systemic/blood , Adult , Aged , Biomarkers/blood , Female , Humans , Male , Middle Aged , Prognosis , Sensitivity and Specificity , Severity of Illness IndexABSTRACT
Differences among angiotensin-converting enzyme inhibitors (ACEI) in scavenging reactive oxygen species were described and mainly attributed to the presence or absence of a thiol group. Plasma constituents and red cells are known targets for oxidative damage. Transition metals, like copper, are well known catalizers of free radical generation. In the present study we compared the abilities of captopril (a thiol ACEI), enalaprilat, and lisinopril (two nonthiol ACEI) for inhibiting copper-induced thiobarbituric acid reactive substances (TBARS) formation and fluorescence generation in whole human plasma and low-density lipoprotein. The effects of those ACEI on copper/hydrogen peroxide-induced fluorescence development and electrophoretic mobility modification in albumin and on copper-induced TBARS formation and hemolysis in human red cells were also compared. Captopril was more effective than the two nonthiol ACEI in inhibiting plasma and LDL lipid peroxidation, but it was ineffective in inhibiting the albumin oxidative modification that was moderately inhibited by enalaprilat and lisinopril. On the contrary, the inhibitory effects of the three ACEI on copper-induced lipid peroxidation and hemolysis in red cell suspensions were more uniform. This as yet unreported red cell protective effect may deserve pharmacological evaluation. Our results show that captopril is a more effective antioxidant than the nonthiol ACEI in some systems. However, the nonthiol ACEI also have the ability to partially protect some targets against oxidative damage. These observations suggest that the presence of a thiol group in the ACEI structure is not the only determinant for the antioxidant properties.
Subject(s)
Angiotensin-Converting Enzyme Inhibitors/pharmacology , Copper/pharmacology , Lipid Metabolism , Lipid Peroxidation/drug effects , Proteins/metabolism , Sulfhydryl Compounds/pharmacology , Erythrocytes/metabolism , Fluorescence , Hemolysis/drug effects , Hydrogen Peroxide/pharmacology , Hydrogen-Ion Concentration , Oxidation-Reduction , Serum Albumin, Bovine/chemistryABSTRACT
Xanthine oxidase exists in vivo predominantly as a NAD(+)-dependent dehydrogenase form (xanthine dehydrogenase) which can be transformed into oxygen-dependent oxidase forms as a result of sulfhydryl oxidation (reversible xanthine oxidase) or proteolysis (irreversible xanthine oxidase). Xanthine oxidase has been hypothesized to be a potential source of oxygen-derived free radicals during reperfusion of ischemic tissues. Xanthine dehydrogenase was purified from rat liver and converted into reversible xanthine oxidase by heating at 37 °C and into irreversible xanthine oxidase by proteolysis with trypsin. Silibinin and bendazac are compounds used in therapeutics and to which free radical scavenging properties were ascribed. The effects of the compounds silibinin and bendazac on the different forms of the enzyme were studied. Silibinin inhibited all the forms of the enzyme but bendazac inhibited only reversible and irreversible xanthine oxidase. The inhibitions seem to be mixed non-competitive-competitive. The authors discuss the hypothesis that selective inhibitors of xanthine oxidase, preventing the interruption of uric acid formation, may have some advantage over the inhibitors of both xanthine dehydrogenase and xanthine oxidase in the treatment and prevention of situations such as ischemia and reperfusion syndromes.
ABSTRACT
The Author reviews the pathological consequences of chronic alcoholism with particular emphasis to the consequences of malnutrition, especially vitamin B-1 deficiency, immune deficiencies, adaptation of cytochrome 2E1, testicular atrophy, alterations of the skin, bones, liver, muscles, cardiac and neuropsychiatric.
Subject(s)
Alcoholism , Deficiency Diseases/etiology , Alcoholism/complications , Alcoholism/metabolism , Cardiomyopathy, Alcoholic/etiology , Deficiency Diseases/metabolism , Female , Humans , Immunologic Deficiency Syndromes/etiology , Liver Diseases, Alcoholic/etiology , Male , Nutrition Disorders/etiologyABSTRACT
The aim of the present study was to confirm the increase of adenosine deaminase (ADA) activity in progressive systemic sclerosis (PSS), described by Sasaki & Nakajima, and to compare plasma ADA activity of patients in different stages of the disease. Enzyme activity was measured with a colorimetric assay. The 48 patients were subdivided into 3 groups: subgroup 1 (n = 10), disease still limited to the skin; subgroup 2 (n = 21), involvement of the skin and oesophagus; and subgroup 3 (n= 17), involvement of the skin and multiple internal organs. ADA levels were highest in subgroup 3. However, the difference with respect to subgroup 2 did not reach statistical significance. Subgroup 1 was different from controls and subgroups 2 and 3 (p<0.001). Our results confirm that ADA activity is increased in PSS, and that this finding is observed even in the early stages of the disease process. We speculate that the increase in ADA, a well-known marker of T-cell activation, might be an indicator of disease activity in PSS, in the beginning as well as during phases of exacerbation in later stages of the disease.