ABSTRACT
The advent of inexpensive computers and associated control and data acquisition software makes possible the development of sophisticated, configurable, integrated monitoring and control systems for small-scale laboratory and pilot-scale fermentors at low cost. We describe here the implementation of such a system, the interfacing of off-line instruments to enhance real time data analysis, low level process control and several substrate feeding protocols.
Subject(s)
Escherichia coli/metabolism , Fermentation , Monitoring, Physiologic/methods , Ammonia/metabolism , Carbon Dioxide/metabolism , Computer Systems , Electronic Data Processing , Glucose/metabolism , Hydrogen-Ion Concentration , Oxygen/metabolism , SoftwareSubject(s)
Hemoglobins/biosynthesis , Chromatography, Ion Exchange , Escherichia coli/genetics , Fermentation , Gene Expression , Globins/genetics , Hemoglobins/genetics , Hemoglobins/isolation & purification , Humans , Plasmids/genetics , Recombinant Proteins/biosynthesis , Recombinant Proteins/genetics , Recombinant Proteins/isolation & purification , Restriction MappingABSTRACT
A fluoroacetate-resistant mutant of Escherichia coli K-12 (MM-294) accumulated less acetate in the medium during growth to high cell density in fermentor cultures and was shown to be defective in its phosphotransacetylase activity. The mutant had an improved ability to continue growing during induction of interleukin-2 (IL-2) synthesis, and in fermentor cultures it gave a higher level of specific IL-2 accumulation than its parent during expression under control of the temperature-sensitive pL promoter. In flask cultures at lower cell density, the mutant again produced less acetate than the parent, although both showed a much lower level of acetate accumulation than that seen in fermentors at high cell density. Both showed a higher specific expression level of IL-2 in flask cultures, and there was a greater difference between the mutant and its parent in the final extent of specific IL-2 accumulation in fermentor cultures compared with flask cultures. Thus, the concentration of acetate in the medium, which was much higher in fermentor cultures (greater than or equal to 300 mM after 5 h of induction) than in flask cultures (less than or equal to mM) of the parent organism, was a significant factor in limiting expression of the heterologous protein product, IL-2. The acetate kinase-phosphotransacetylase pathway was therefore a major source of acetate formation in these cultures. Blocking this pathway improved accumulation of IL-2 and did not slow growth.
Subject(s)
Acetyltransferases/analysis , Escherichia coli/metabolism , Interleukin-2/biosynthesis , Phosphate Acetyltransferase/analysis , Acetates/metabolism , Ethanol/metabolism , Fermentation , Mutation , Phosphate Acetyltransferase/genetics , Pyruvates/metabolism , Pyruvic AcidABSTRACT
We examined the ability of transformed Escherichia coli cells in fermentor cultures to accumulate interleukin-2 (IL-2) intracellularly under temperature-regulated control of the phage lambda pL promoter. Induction of expression was undertaken at different culture optical densities, and specific IL-2 accumulation was found to decrease with increasing cell density at induction. Induction at higher culture optical densities was also accompanied by decreased growth during induction and increased acetate accumulation in the culture medium. Experiments were undertaken to study the effect of replacing spent medium by perfusion with fresh medium both before induction and during IL-2 expression at high cell density. Improved IL-2 expression was seen only when perfusion was continued past 1.6 h after the start of induction, and it was accompanied by a significant reduction in acetate buildup. Further improvements were not seen when perfusion was continued beyond hour 3 of induction. Replenishing medium components and decreasing the concentration of diffusible inhibitors before induction did not alleviate acetate buildup, growth limitation, or limitation of IL-2 synthesis. These results suggested that accumulation of diffusible inhibitors such as acetate during induction may be a significant factor limiting IL-2 expression in high-density cultures, but other factors intrinsic to the organism or the protein also played a major role.
Subject(s)
Escherichia coli/genetics , Interleukin-2/genetics , Acetates/metabolism , Acetic Acid , Cell Division , Colony Count, Microbial , Culture Media , Escherichia coli/cytology , Escherichia coli/metabolism , Fermentation , Gene Expression Regulation, Bacterial , Humans , Interleukin-2/biosynthesis , Transformation, GeneticABSTRACT
In continuing studies of patulin biosynthesis, the first enzyme of the pathway, 6-methylsalicylic acid synthetase, was found to be far more labile than were the later enzymes of the pathway. Attempts were made to stabilize 6-methylsalicylic acid synthetase in vitro. The combined addition of the cofactor NADPH, the substrates acetyl-CoA and malonyl-CoA, the reducing agent dithiothreitol, and the proteinase inhibitor phenylmethylsulfonyl fluoride to cell-free extracts was found to prolong the half-life of the enzyme as much as 12-fold. This suggested that proteolysis and the conformational integrity of the enzyme may play an important role in controlling the duration of antibiotic biosynthesis in vivo. This was in agreement with the finding that the intracellular proteinase content of antibiotic-producing cells of Penicillium urticae rapidly increased just before the loss of 6-methylsalicylic acid synthetase content. These in vitro stabilization studies have provided some insight into the metabolic conditions that may stabilize these enzymes in vivo, and into possible ways of extending the life of these catalysts.
Subject(s)
Acyltransferases/metabolism , Ligases/metabolism , Multienzyme Complexes/metabolism , Oxidoreductases/metabolism , Penicillium/enzymology , Acetyl Coenzyme A/pharmacology , Acyltransferases/antagonists & inhibitors , Ascorbic Acid/pharmacology , Dithiothreitol/pharmacology , Drug Stability , Half-Life , Hydrogen-Ion Concentration , Kinetics , Ligases/antagonists & inhibitors , Malonyl Coenzyme A/pharmacology , Multienzyme Complexes/antagonists & inhibitors , NADP/pharmacology , Oxidoreductases/antagonists & inhibitors , Patulin/biosynthesis , Peptide Hydrolases/metabolism , Phenylmethylsulfonyl Fluoride/pharmacology , Protein Conformation/drug effects , Sulfhydryl CompoundsABSTRACT
A procedure was developed for purification of ornithine transcarbamylase (OTCase) to near homogeneity from Bacillus subtilis 168. The purified native enzyme existed as a mixture of dimeric, tetrameric, and hexameric forms, but was converted to the dimer in the presence of 2-mercaptoethanol. The molecular weight of the subunit was 44,000. Some general kinetic properties of the enzyme were described. OTCase was repressed by arginine in growing B. subtilis cells, but the enzyme was induced by arginine at the end of exponential growth. Specific antibodies against the purified OTCase were used to show that the same enzyme was produced under all conditions. These results and studies of a mutant lacking OTCase demonstrated that B. subtilis produced only a single OTCase. OTCase was clearly required for arginine biosynthesis, but the physiological function of OTCase induction by arginine was obscure. OTCase was not induced by, or required for, growth on arginine as a carbon and nitrogen source. Absence of OTCase in a mutant did not alter the yield or arginine content of its spores in comparison to a strain containing OTCase.
Subject(s)
Bacillus subtilis/enzymology , Ornithine Carbamoyltransferase/isolation & purification , Arginine/metabolism , Bacillus subtilis/growth & development , Bacillus subtilis/metabolism , Kinetics , Molecular Weight , Ornithine Carbamoyltransferase/metabolism , Precipitin Tests , Spores, Bacterial/enzymologyABSTRACT
When Bacillus subtilis cells grew and sporulated on glucose-nutrient broth, ornithine transcarbamylase (OTCase) was synthesized in the early stationary phase and then inactivated. The loss of OTCase activity was much slower in a mutant that was deficient in a major intracellular serine protease (ISP). Immunochemical analysis showed that synthesis of OTCase decreased to a low, but detectable, level during its inactivation and that loss of activity was paralleled by loss of cross-reactive protein. Because the antibodies were capable of detecting denatured and fragmented forms of OTCase, we conclude that inactivation involved or was rapidly followed by degradation in vivo. Native OTCase was not degraded in crude extracts or when purified ISP and OTCase were incubated together under a variety of conditions. Synthesis of OTCase was not shut off normally in the ISP-deficient mutant. When the effects of continued synthesis were minimized, OTCase was degraded only slightly slower in the mutant than in its parent. Thus, the mutant had unanticipated pleiotropic characteristics, and it was unlikely that ISP played a major role in the degradation of OTCase in vivo.