ABSTRACT
El Laboratorio de Análisis Clínico del INLASA realizó el Control de Calidad Instrumental de los espectrofotómetros de 8 Laboratorios de la ciudad de La Paz,Bolivia pertenecientes a la Red Departamental de Química Clínica. Se evaluó la Exactitud Fotométrica realizando 3 lecturas: la primera lectura se realizó en UV a 340 nm con solución de dicromato de potasio 50 mg/1 en ácido sulfúrico 0.01N, la segunda en Visible a 510 nm con solución de nitrato de cobalto 0.0918 M en medio sulfúrico y la tercera lectura en Visible a 650 nm con solución de sulfato de cobre 20 g/1 en medio sulfúrico. También se evaluó la Linealidad Fotométrica realizando la lectura en UV a 340 nm con 4 soluciones de diferente concentración de dicromato de potasio, absorvancia independiente de la temperatura. En el Control de la Exactitud Fotométrica los resultados obtenidos mostraron que 3 espectrofotómetros dieron valores dentro del rango óptimo, uno dentro del rango aceptable y cuatro fuera del rango aceptable. En el Control de la Linearidad Fotométrica, los resultados obtenidos con la lectura UV a 340 nm con las 4 soluciones de Dicromato de Potasio mostraron que sólo 2 espectrofotómetros cumplieron con la linearidad respectiva. El Control Instrumental realizado en los 8 espectrofotómetros, nos permitió evaluar su funcionamiento, habiéndose observado que es necesario realizar el mantenimiento permanente de los equipos para garantizar su confiabilidad
Subject(s)
Quality Control , SpectrophotometersABSTRACT
The human adult intestinal epithelium has traditionally been described as nonpermeable to proteins. However, indirect evidence suggests that reduced absorption of undegraded proteins might take place under physiological conditions. Using bromelain (an enzyme obtained from pineapple stems) as a model protein, we studied the extent of this mucosal permeation in 19 healthy men. The protein was detected in plasma by immunoassay and by its proteolytic activity after oral administration. The estimated plasma half-life was 6-9 h. After oral multidosing (3 g/day), plasma concentration reached as much as 5,000 pg/ml by 48 h. From the plasma concentration curve, it could be estimated that an average of 10.8 micrograms of bromelain was present in plasma in the 3- to 51-h period. The presence of undegraded bromelain in plasma was shown unequivocally by immunoprecipitation of plasma samples with antibromelain antibodies, followed by gel electrophoresis and immunodetection. Moreover, the enzyme retained its biological activity, at least in part. Circulating bromelain was found associated with alpha 2-macroglobulin and alpha 1-antichymotrypain. The results of this work confirm the existence of a small but significant intestinal transport of undegraded proteins in healthy men.
Subject(s)
Bromelains/pharmacokinetics , Dietary Proteins , Intestinal Absorption , Intestinal Mucosa/physiology , Administration, Oral , Adult , Bromelains/administration & dosage , Bromelains/blood , Double-Blind Method , Half-Life , Humans , Male , Metabolic Clearance Rate , Random Allocation , alpha 1-Antitrypsin/metabolism , alpha-Macroglobulins/metabolismABSTRACT
UNLABELLED: PET permits the quantification of myocardial blood flow, but is hampered by the limited spatial resolution of PET images. METHODS: We evaluated two methods for the correction of resolution effects in PET perfusion 13NH3-ammonia images. In one model, the spillover and recovery coefficients are estimated in the kinetic modeling analysis. The new, second model uses an explicit delineation of the left ventricular wall and a convolution model for the system point spread function to compute the regional values of the spillover and recovery coefficients. RESULTS: The new method is validated with phantom measurements. The two methods are evaluated on animal experiments using 13NH3-ammonia. Both two- and three- compartment models were used to compute absolute flow values. Excellent linear correlations with microsphere data were obtained. The slope of the regression line was lower for corrections based on kinetic modeling as compared to convolution-based correction. In animal experiments, recovery coefficients of 59% for the myocardial wall and 86% for the blood pool were obtained. Spillover from the blood pool into the myocardial was was 14%. CONCLUSION: The new correction method strongly suppresses spillover and recovery effects due to limited resolution.
Subject(s)
Algorithms , Ammonia , Artifacts , Heart/diagnostic imaging , Nitrogen Radioisotopes , Tomography, Emission-Computed , Animals , Coronary Circulation/physiology , Dogs , Female , Humans , Linear Models , Male , Models, Cardiovascular , Phantoms, Imaging , Ventricular Function/physiologyABSTRACT
The wave model of endogenous psychoses constructed by us is based on special properties of the envelopes of arterial pulse waves. It has been tested and used by the Bechterev Institute in Leningrad. We could confirm us that all examined patients are classified into the right class of psychosis solely by their measurement vector of the envelope curve. Also statements about the progress of the psychosis are possible.
Subject(s)
Psychotic Disorders/diagnosis , Pulse , Telemetry , Analysis of Variance , Arteries/physiopathology , Bipolar Disorder/diagnosis , Bipolar Disorder/physiopathology , Depressive Disorder/diagnosis , Depressive Disorder/physiopathology , Evoked Potentials , Humans , Psychotic Disorders/physiopathologyABSTRACT
After the successful classification of endogenous psychoses only by a vector of measuring dates of covering curves of the arterial pulse waves was tested, whether the concrete state of activity of the generally activating reticular system observed in this case may be described also according to the peculiarity of centrally regulated variations of the cardiac rhythm. Using the vectors of the measuring values of these regulation influences we also got a rate of correct classification of endogenous psychoses of about 80%.
Subject(s)
Psychotic Disorders/classification , Pulse , Analysis of Variance , Computers , Depressive Disorder/physiopathology , Electrocardiography , Humans , Schizoid Personality Disorder/physiopathology , Schizophrenia/physiopathologyABSTRACT
The envelope curves of arterial pulse waves are carries of information about the central circulatory and respiratory regulation. Using a discriminence analysis, the mean width of the wave peak of this curve appears as a characteristic feature of the activity form of the generally activating reticular system which is changed in endogenous depressions. By means of this feature, it is possible to distinguish patients with endogenous depression from healthy test persons without using psychopathological findings.
Subject(s)
Depression/physiopathology , Pulse , Adult , Depression/diagnosis , Female , Humans , Male , Middle AgedABSTRACT
As an example are represented in three processes of disease of Shy-Drager-Syndrom pathogenesis, diagnosis, and therapy. The leading symptom manifested in asympathicotonical hypotonical state is mixed with vegetative and centralnervious changes. The manifestation of this disease after the age of fourty makes it to a very strange illness of the second half of life.
Subject(s)
Autonomic Nervous System Diseases/diagnosis , Shy-Drager Syndrome/diagnosis , Aged , Bandages , Caffeine/therapeutic use , Dexamethasone/therapeutic use , Erectile Dysfunction/complications , Facial Paralysis/complications , Female , Humans , Hypohidrosis/complications , Hypotension, Orthostatic/complications , Male , Middle Aged , Muscle Rigidity/complications , Prednisone/therapeutic use , Shy-Drager Syndrome/therapyABSTRACT
In comparison to normal children of the same age, in oligophrenic children there were qualitatively special structures of the intellectual mnestic functions, attention, auditory and visual perception, higher emotions and brain bioelectric activity. These data have an importance for the diagnosis and elaboration of standarized indices of oligophrenic patients in preschool age.