ABSTRACT
Brown spot (BS) disease causes significant losses to rice productivity. In this study, a roving survey in the Karnataka state of India revealed a wider distribution of BS with a percent disease index range of 20.56-50.74. From the symptomatic geo-distinct samples, pure cultures of 63 isolates were obtained. Based on the conidial morphology, 63 isolates were identified as Bipolaris oryzae (Bo) (n = 40), Curvularia lunata (Cl) (n = 15), and Exserohilum rostratum (Er) (n = 08). The taxonomic identity was further confirmed via ITS-sequencing. A pathogenicity assay on a BS-susceptible rice cultivar GNV-05-01 confirmed the pathogenicity of all three pathogens, which induces typical BS disease on test plants. Further, on PDA media, all isolates of three pathogens showed significant cultural diversity for mycelial color, colony type, and sporulation. We further studied the in-planta distribution of three pathogens on a randomly collected 600 BS spots from 10 different rice fields, which indicated that 77.83%, 17.33%, and 4.83% of the typical BS were produced by Bo, Cl, and Er, respectively. The ITS region was sequenced for selected 9, 7, and 3 isolates of Bo, Cl, and Er, respectively, and analyzed for their nucleotide and haplotype diversity, and phylogenetic relationships. A phylogenetic study identified the unique clustering patterns, and haplotyping indicated 3, 4, and 6 haplotypes. Tajima's D (D) test showed several rare alleles in the ITS regions. This is the first comprehensive study reporting the three fungal pathogens causing BS of rice and it is useful for re-designing the screening protocol for the host plant resistance breeding program. Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-024-04033-3.
ABSTRACT
False smut disease of rice caused by Ustilaginoidea virens, is an emerging threat to rice cultivation worldwide due to its detrimental effects on grain yield and quality. False smut disease severity was 4.44â17.22% during a roving survey in Kharif 2016 in the four different rice ecosystems of Karnataka, India. Further, 15 pathogen isolates representing four different ecosystems were studied for their virulence and morphometric diversity. Among the 15 strains studied, most virulent strains Uv-Gvt was selected for whole genome sequencing in Illumina NextSeq 500 platform using 2 × 150 bp sequencing chemistry. The total assembled genome of Uv-Gvt was 26.96 Mb, which comprised of 9157 scaffolds with an N50 value of 15,934 bp and 6628 protein-coding genes. Next, the comparative genomic study revealed a similar gene inventory as UV-8b and MAFF 236576 strains reported from China and Japan, respectively. But, 1756 genes were unique to Uv-Gvt strain. The Uv-Gvt genome harbors 422 putative host-pathogen interacting genes compared to 359 and 520 genes in UV-8b and MAFF 236576 strains, respectively. The variant analysis revealed low genetic diversity (0.073â0.088%) among U. virens strains. Further, phylogenetic analysis using 250 single copy orthologs genes of U. virens revealed a distinct phylogeny and an approximate divergence time. Our study, report the genomic resource of rice false smut pathogen from India, where the disease originated, and this information will have broader applicability in understanding the pathogen population diversity.