ABSTRACT
The objective of the study was to determine interval values of biochemical parameters in the most commonly applied experimental model among different species, i.e. rats. Blood analysis of experimental animals is done in different research fields. They are important especially in experiments in pharmacology, pathophysiology, experimental surgery, toxicology and for monitoring experimental disorders in laboratory animals. In this paper, basic biochemical markers in the blood serum of Buffalo and Wistar rats are also compared in relation to the animals' age and sex. The values were obtained using the latest available measurement methods and the above-listed checkpoints were considered. The biochemical markers show variability between the particular groups of animals related to their age, sex, and strain. The obtained data may be used to create a model of interval values of biochemical parameters for the Buffalo and Wistar rat strains. This study is necessary to enhance our understanding on basic parameters in these animals which are often used in different medical experiments.
Subject(s)
Aging/physiology , Blood Glucose , Blood Proteins , Blood Urea Nitrogen , Electrolytes/blood , Sex Characteristics , Alanine Transaminase/blood , Alkaline Phosphatase/blood , Animals , Aspartate Aminotransferases/blood , Bilirubin/blood , Cholesterol/blood , Female , Male , Rats , Rats, Inbred BUF , Rats, WistarABSTRACT
Glyceraldehyde-3-phosphate dehydrogenase is a glycolytic enzyme that catalyses conversion of glyceraldehyde-3-phosphate to 1,3-diphosphoglycerate. ATP has been found to have an inhibitory effect on this enzyme. To establish the interaction between the enzyme and ATP, a fluorescence technique was used. Fluorescence quenching in the presence of ATP suggests cooperative binding of ATP to the enzyme (the Hill obtained coefficient equals 2.78). The interaction between glyceraldehyde-3-phosphate dehydrogenase and ATP may control not only glycolysis but other activities of this enzyme, such as binding to the cytoskeleton.
Subject(s)
Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism , Myocardium/enzymology , Adenosine Triphosphate/metabolism , Animals , Cattle , Chromatography, Ion Exchange , Glyceraldehyde-3-Phosphate Dehydrogenases/chemistry , Glyceraldehyde-3-Phosphate Dehydrogenases/isolation & purification , Kinetics , Papillary Muscles/enzymology , Spectrometry, FluorescenceABSTRACT
Two subtypes of angiotensin II receptors have been characterised so far: AT1 and AT2. In PC12W pheochromocytoma cells, only AT2 receptors have been found (acting probably through G1 proteins or via G protein-independent mechanism). Here, dynamic changes in phosphorylation pattern in PC12W cells upon induction of angiotensin II and under influence of redox agents were investigated. PC12W pheochromocytoma cell line was preincubated with angiotensin II, then incubated with redox agents. After lysis the cells were subjected to Western-Blotting technique with antiphosphotyrosine and anti-ERK2 antibodies, as well as phosphotyrosine phosphatases and kinases activity was measured. Angiotensin II through its AT2 receptor induced dephosphorylation of tyrosines of the proteins in the range of 60 to 150 kD in PC12W cells. The obtained phosphorylation pattern suggests that AT2 receptors may act comparably to leukocyte CD45 receptor pathway. Treatment of PC12W cells with H2O2 resulted in significant decrease in phosphotyrosine phosphatases activity. It could be assumed that signal transduction based on protein phosphorylation might be controlled by cellular redox mechanisms.