Contamination of Bisphenol A, Nonylphenol, Octylphenol, and Estrone in Major Rivers of Mega Manila, Philippines.

Endocrine-disrupting compounds (EDCs) such as natural and synthetic hormones as well as phenolic industrial chemicals are considered contaminants of emerging concern in environmental waters. While EDCs carried through rivers may impact ecosystem health and productivity, these compounds are still not widely studied nor regulated. In the present study, we report the occurrence of EDCs in urban rivers in Mega Manila, namely, the Marikina, Pasig, Angat, and Pampanga Rivers that drain into Manila Bay. Endocrine-disrupting compounds may have reached these rivers through domestic wastewater and industrial effluents. Water samples from the rivers were extracted by solid-phase extraction before instrumental analysis using a liquid chromatograph coupled to a mass spectrometer. The analytical method exhibited good linear response (>99% in the concentration range of 1-50 µg/L) and low instrument detection limits (0.14-1.46 µg/L) for the hormones estrone (E1), estradiol, ethinylestradiol, progesterone, and testosterone, and the industrial chemicals bisphenol A, nonylphenol, and octylphenol. Of the hormones, E1 was detected up to 11 ng/L. Bisphenol A, nonylphenol, and octylphenol were measured up to 54, 1878, and 62 ng/L, respectively. Endocrine-disrupting compounds are not yet monitored in water bodies in the Philippines and there are no local guidelines yet on occurrence, pollution prevention, and mitigation. Environ Toxicol Chem 2024;43:259-266. © 2023 SETAC.

ß-Lactam antibiotics and antibiotic resistance in Asian lakes and rivers: An overview of contamination, sources and detection methods.

Lakes and rivers are sources of livelihood, food and water in many parts of the world. Lakes provide natural resources and valuable ecosystem services. These aquatic ecosystems are also vulnerable to known and new environmental pollutants. Emerging water contaminants are now being studied including antibiotics because of the global phenomenon on antibiotic resistance. ß-Lactam antibiotics are widely used in human and animal disease prevention or treatment. The emergence of antibiotic resistance is a public health threat when bacteria become more resistant and infections consequently increase requiring treatment using last resort drugs that are more expensive. This review summarizes the key findings on the occurrence, contamination sources, and determination of ß-lactam antibiotics and ß-lactam antibiotic resistant bacteria and genes in the Asian lake and river waters. The current methods in the analytical measurements of ß-lactam antibiotics in water involving solid-phase extraction and liquid chromatography-mass spectrometry are discussed. Also described is the determination of antibiotic resistance genes which is primarily based on a polymerase chain reaction method. To date, ß-lactam antibiotics in the Asian aquatic environments are reported in the ng/L concentrations. Studies on ß-lactam resistant bacteria and resistance genes were mostly conducted in China. The occurrence of these emerging contaminants is largely uncharted because many aquatic systems in the Asian region remain to be studied. Comprehensive investigations encompassing the environmental behavior of ß-lactam antibiotics, emergence of resistant bacteria, transfer of resistance genes to non-resistant bacteria, multiple antibiotic resistance, and effects on aquatic biota are needed particularly in rivers and lakes that are eventual sinks of these water contaminants.

Occurrence and distribution of hormones and bisphenol A in Laguna Lake, Philippines.

The occurrence of hormones and hormone-like compounds in water has been regarded as an emerging environmental concern. These water contaminants are known to cause endocrine disruption that may affect aquatic organisms. In this study, hormones and phenolic xenoestrogens were investigated and measured in the surface waters of Laguna Lake, Philippines. Laguna Lake is the largest lake in the country that has many uses including fish cultivation and source for potable water production. The hormones estrone, 17-beta-estradiol, 17-alpha-ethynylestradiol, progesterone and testosterone as well as the plasticizer bisphenol A in the lake water were determined by solid phase extraction and LC-MS/MS. The extraction method exhibited good recoveries (75-90% in spiked lake water) while the instrumental method of analysis has low detection limits (0.01-0.24 µg/L) and good linear response (>99% in the 0-50 µg/L concentration range). In the nine sampling sites across the lake, estrone was found in concentrations between 0.03 and 0.30 ng/L. 17-Beta-estradiol, testosterone and progesterone were detected in some of the sites in concentrations up to 0.36, 0.22, and 2.05 ng/L, respectively. Bisphenol A was detected in all sites in higher concentrations of 0.71-47.40 ng/L. Although there are no local guidelines yet, the determination and monitoring of these emerging water contaminants are important because of their potential environmental impacts. Further to this initial study, investigations on point sources spanning the tributary rivers, long-term determination of locational and temporal variations, and assessment of ecotoxicological risks are needed. These are crucial in the regulation and mitigation of discharges into the lake.