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1.
Sci Total Environ ; 954: 176652, 2024 Oct 01.
Article in English | MEDLINE | ID: mdl-39362537

ABSTRACT

The Tibetan Plateau is one of the highest and most pristine plateaus in the world, and its ecological environment has a significant impact on global climate and the distribution of water resources. Mercury (Hg), as a toxic metal pollutant, can have a severe impact on the health of living organisms and the ecosystem due to its presence in the environment. This study collected 336 soil samples from 28 sites across four typical surface vegetation landscapes (meadow, grassland, desert, and forest) on the Tibetan Plateau to measure soil THg (Total Hg) concentrations. The research aimed to explore the factors influencing soil THg levels, analyze pollution and environmental risks of THg in the surface soil, and evaluate the associated health risks to the local population. The results indicate that the mean soil THg concentration (31.84 ± 32.58 ng·g-1) of this study is compared to the background value of THg in Tibetan Plateau soils (37.0 ng·g-1), but there are significant differences in THg concentration among soils with different surface vegetation landscapes. The mean THg concentration in soils of forest vegetation types (74.42 ± 41.19 ng·g-1) is approximately twice the background value of Tibetan Plateau soils. In the forested regions of the southeastern, eastern, and southern Tibetan Plateau, soil concentrations of total mercury are relatively high, whereas in the desert areas of the northern, northwestern, and northeastern Tibetan Plateau, the concentrations are lower. Organic matter (soil organic carbon) being an important factor influencing the soil THg. Based on existing surface soil THg data from this and previous research in Tibetan Plateau (n = 477), 34.2 % of the samples show Hg pollution and potential ecological risks. However, the health risks of soil Hg to both adults and children are not significant.

2.
Inorg Chem ; 62(23): 9158-9167, 2023 Jun 12.
Article in English | MEDLINE | ID: mdl-37243623

ABSTRACT

Photocatalytic degradation of organic pollutants via semiconductors with high visible light response and effective carrier separation is an economical and green route to greatly achieve environmental remediation. Herein, an efficient BiOI/Bi2MoO6 p-n heterojunction was in situ fabricated through hydrothermal method by substituting Mo7O246- species for I ions. The characteristic p-n heterojunction exhibited a strongly enhanced visible light responsive absorption from 500 to 700 nm owing to the narrow band gap of BiOI and a greatly effective separation of photoexcited carriers because of the built-in electric field on the interface between BiOI and Bi2MoO6. Moreover, the flower-like microstructure also promoted the adsorption of organic pollutants owing to the large surface area (about 10.36 m2/g), good for further photocatalytic degradation. As a result, BiOI/Bi2MoO6 p-n heterojunction showed an excellent photocatalytic activity of RhB of almost 95% in a short time of 90 min under wavelength longer than 420 nm, 2.3 and 2.7 times higher compared with single BiOI and Bi2MoO6, respectively. This work offers a promising approach to purify the environment through the utilization of solar energy by constructing efficient p-n junction photocatalysts.

3.
Front Microbiol ; 13: 1041338, 2022.
Article in English | MEDLINE | ID: mdl-36466668

ABSTRACT

Human respiratory syncytial virus (RSV) is a ubiquitous pediatric pathogen causing serious lower respiratory tract disease worldwide. No licensed vaccine is currently available. In this work, the coding gene for mDS-Dav1, the full-length and prefusion conformation RSV fusion glycoprotein (F), was designed by introducing the stabilized prefusion F (preF) mutations from DS-Cav1 into the encoding gene of wild-type RSV (wtRSV) F protein. The recombinant adenovirus encoding mDS-Cav1, rChAd63-mDS-Cav1, was constructed based on serotype 63 chimpanzee adenovirus vector and characterized in vitro. After immunizing mice via intranasal route, the rChAd63-mDS-Cav1 induced enhanced neutralizing antibody and F-specific CD8+ T cell responses as well as good immune protection against RSV challenge with the absence of enhanced RSV disease (ERD) in BALB/c mice. The results indicate that rChAd63-mDS-Cav1 is a promising mucosal vaccine candidate against RSV infection and warrants further development.

4.
J Nutr ; 152(6): 1450-1458, 2022 06 09.
Article in English | MEDLINE | ID: mdl-35285912

ABSTRACT

BACKGROUND: Limited evidence supports the efficacy of iron-rich foods (IRFs) in improving iron status during pregnancy. OBJECTIVES: The study aims to evaluate the effect of IRFs on iron status and biomarkers of iron metabolism in the third trimester of pregnancy. METHODS: A total of 240 pregnant women at 11-13 wk of gestation without iron-deficiency anemia (IDA) in South China were recruited to this single-blind clinical trial [non-IDA referred to both hemoglobin (Hb) ≥110g/L and serum ferritin (SF) ≥15ng/mL],  randomly assigned to 1) control, 2) IRFs containing 20 mg iron/d (IRF-20), or 3) IRFs containing 40 mg iron/d (IRF-40). The IRFs were consumed 3 days a week, including pork liver, chicken/duck blood, soybean, and agaric. The IRFs started at recruitment and ended in the predelivery room. Primary outcome included anemia (Hb <110 g/L), iron deficiency (ID, definition 1: SF <15 ng/mL; definition 2: SF <12 ng/mL), and IDA (ID and Hb <110 g/L). Secondary outcome was plasma Hb and iron indices, including SF, serum hepcidin, and iron. RESULTS: All participants who completed the trial with full data (n = 170) were included in the analysis. At the endline, both intervention groups showed lower ID and IDA rates than control. Specifically, IRF-40 showed a lower ID (SF <12 ng/mL) rate than control (9.0% compared with 22.8%, P = 0.022). For IDA by definition 1, the incidence in IRF-40 was lower than that in control (1.9% compared with 8.9%, P = 0.045). For IDA by definition 2, the incidence in IRF-20 was lower than that in control (3.9% compared with 17.9%, P = 0.049). Moreover, IRF-20 showed higher SF concentrations than control (P = 0.039). No effects of IRFs on anemia (P = 0.856), plasma Hb (P = 0.697), serum hepcidin (P = 0.311), and iron (P = 0.253) concentrations were observed. The assessed iron intakes were 22.2 mg/d in IRF-20 and 25.0 mg/d in IRF-40, respectively. CONCLUSIONS: Antenatal IRFs reduce the risk of ID and IDA in late pregnancy, although the present results are inadequate to confirm an ideal dosage (No. ChiCTR1800017574).


Subject(s)
Anemia, Iron-Deficiency , Anemia , Anemia/complications , Female , Hemoglobins/analysis , Hepcidins , Humans , Iron , Pregnancy , Pregnant Women , Single-Blind Method
5.
BMC Pregnancy Childbirth ; 21(1): 50, 2021 Jan 12.
Article in English | MEDLINE | ID: mdl-33435921

ABSTRACT

BACKGROUND: Antenatal corticosteroids (ACS) treatment is critical to support survival and lung maturation in preterm infants, however, its effect on feeding and growth is unclear. Prior preterm delivery, it remains uncertain whether ACS treatment should be continued if possible (repeated course ACS), until a certain gestational age is reached. We hypothesized that the association of single-course ACS with feeding competence and postnatal growth outcomes might be different from that of repeated course ACS in very-low-birth-weight preterm infants. METHODS: A multicenter retrospective cohort study was conducted in very-low-birth-weight preterm infants born at 23-37 weeks' gestation in South China from 2011 to 2014. Data on growth, nutritional and clinical outcomes were collected. Repeated course ACS was defined in this study as two or more courses ACS (more than single-course). Infants were stratified by gestational age (GA), including GA < 28 weeks, 28 weeks ≤ GA < 32 weeks and 32 weeks ≤ GA < 37 weeks. Multiple linear regression and multilevel model were applied to analyze the association of ACS with feeding and growth outcomes. RESULTS: A total of 841 infants were recruited. The results, just in very-low-birth-weight preterm infants born at 28-32 weeks' gestation, showed both single and repeated course of ACS regimens had shorter intubated ventilation time compared to non-ACS regimen. Single-course ACS promoted the earlier application of amino acid and enteral nutrition, and higher rate of weight increase (15.71; 95%CI 5.54-25.88) than non-ACS after adjusting for potential confounding factors. No associations of repeated course ACS with feeding, mean weight and weight increase rate were observed. CONCLUSIONS: Single-course ACS was positively related to feeding and growth outcomes in very-low-birth-weight preterm infants born at 28-32 weeks' gestation. However, the similar phenomenon was not observed in the repeated course of ACS regimen.


Subject(s)
Adrenal Cortex Hormones/therapeutic use , Betamethasone/therapeutic use , Infant, Very Low Birth Weight/growth & development , Prenatal Care , Respiratory Distress Syndrome, Newborn/prevention & control , Adrenal Cortex Hormones/administration & dosage , Betamethasone/administration & dosage , China , Cohort Studies , Female , Gestational Age , Humans , Infant, Newborn , Pregnancy , Retrospective Studies
7.
Appl Biochem Biotechnol ; 191(3): 1340-1352, 2020 Jul.
Article in English | MEDLINE | ID: mdl-32100232

ABSTRACT

The aim of this study was to explore whether or not acetylresveratrol as a potential substitute for resveratrol dragged the toxic aldehyde to inhibit the mutation of mitochondrial DNA. The results revealed that the acetylresveratrol shifted ultraviolet peak of trans-crotonaldehyde from 316 to 311 nm. In mitochondria, the acetylresveratrol split the ultraviolet peak at 311 nm of trans-crotonaldehyde into 311 nm and 309 nm; the aldehyde Raman band of trans-crotonaldehyde was red shifted by the acetylresveratrol from 1689 to 1686 cm-1 with obvious band decline; Raman bands at 1149 cm-1, 1168 cm-1, and 1325 cm-1 of acetylresveratrol disappeared. In aldehyde dehydrogenase, the aldehyde Raman band of trans-crotonaldehyde was red shifted by the acetylresveratrol from 1689 to 1684 cm-1 with band decline; Raman bands at 1150 cm-1, 1168 cm-1, and 1324 cm-1 of acetylresveratrol declined. The weak acidic microenvironment was the best, for the acetylresveratrol dragged the toxic aldehyde of trans-crotonaldehyde. Compared with the resveratrol, the effect of the acetylresveratrol on the toxic aldehyde of trans-crotonaldehyde was very similar to that of the resveratrol. The acetylresveratrol is very suitable as a potential substitute for resveratrol dragged the toxic aldehyde to inhibit the mutation of mitochondrial DNA. Graphical Abstract In mitochondria, the Raman band of the toxic -CH=O of trans-crotonaldehyde (TCA) dragged by the Acetyl-Res from 1689 to 1686 cm-1 with obvious band decline, while the Raman bands at 1149 cm-1, 1168 cm-1, and 1325 cm-1 of the Acetyl-Res disappeared, respectively. The Acetyl-Res is very suitable as a potential substitute, for the Res dragged the toxic -CH=O of TCA to inhibit the mutation of mitochondrial DNA for anticancer.


Subject(s)
Aldehydes/chemistry , DNA, Mitochondrial/genetics , Mitochondria, Heart/drug effects , Mutation , Resveratrol/analogs & derivatives , Resveratrol/pharmacology , Animals , Hydrogen-Ion Concentration , Male , Protein Domains , Rats , Solvents/chemistry , Spectrum Analysis, Raman , Ultraviolet Rays
9.
10.
AME Case Rep ; 3: 30, 2019.
Article in English | MEDLINE | ID: mdl-31559386

ABSTRACT

The treatment of chronic total occlusion (CTO) of coronary arteries allowed for a remarkable improvement in success rates and is represented by the introduction of the "Hybrid algorithm". An inability to cross the occlusion with a guidewire is the most common cause of failure of CTO revascularization. As antegrade options are limited, intervention upon the extreme angulation of the coronary artery warrants a retrograde approach. Here, we report a 58-year-old man with symptoms of effort angina complicated with three-vessel disease and CTO of the right coronary artery (RCA). Left main artery bifurcation lesions were treated with systematic two-stent strategies and intervention upon RCA CTO after three months by means of a combination of the antegrade and retrograde approaches. The RCA was eventually revascularized by the implantation of stents, and no adverse events were observed during hospitalization.

11.
Sci Rep ; 9(1): 9458, 2019 07 01.
Article in English | MEDLINE | ID: mdl-31263152

ABSTRACT

Rapid production of doubled haploids (DHs) through isolated microspore culture is an important and promising method for genetic study of alfalfa. To induce embryogenesis in alfalfa, isolated microspores were submitted to abiotic stresses during their initial culture, in order to stimulate them to form embryos and plantlets. 'Baoding' and 'Zhongmu No 1' alfalfa cultivars supported reproducible and reliable proliferation response irrespective of any stress treatment of microspores. The microspore developmental stage for isolated microspore culture was studied and we found that uninucleate microspores were best to initiate culture. Exposure of microspores to appropriate low temperature or heat shock stresses were able to increase the efficiency of embryogenesis. The most effective low-temperature treatment was 4 °C for 24 h and the frequency of plantlets induction was 20.0%. The most effective heat shock treatment was 32 °C for 2 d and the frequency of plantlets induction was 14.17%. The analysis of ploidy level performed by flow cytometer revealed that the majority of 278 regenerated plantlets were haploid (65.83%) or doubled haploid (33.81%). This is the first report of haploid production in alfalfa through isolated microspore culture.

12.
IUBMB Life ; 71(7): 1014-1020, 2019 07.
Article in English | MEDLINE | ID: mdl-31012998

ABSTRACT

The aim of this study was to explore how the toxic trans-crotonaldehyde (TCA) in mitochondria or aldehyde dehydrogenase (ALDH) at different pHs was intercepted by oxyresveratrol (Oxy-Res) contributing to anticancer. Ultraviolet-visible (UV-vis) spectroscopy and Raman spectroscopy were employed. UV-vis spectra showed that the Oxy-Res red shifted the peak of the toxic TCA from 316 nm to 325 nm, while the peaks of the Oxy-Res shifted from 329 nm with 290 nm and 300 nm to 325 nm with 303 nm. In the mitochondria, the Oxy-Res blue shifted the peaks of the toxic TCA from 325 nm with 303 nm to 321 nm with 301 nm. Raman spectra revealed that the Oxy-Res caused shifting of the CHO of the toxic TCA from 1,689 cm-1 to 1,671 cm-1 with band decline. The CC of the toxic TCA at 1641 cm-1 was split into 1,639 cm-1 and 1,642 cm-1 with band decline. The bands of the Oxy-Res at 1634 cm-1 , 1,617 cm-1 , and 1,595 cm-1 disappeared. In the mitochondria, the CC of the toxic TCA at 1641 cm-1 splitting disappeared. In ALDH, with the decrease of pH from 7.8 to 6.5, the CHO of the toxic TCA did not red shift from 1,689 cm-1 to 1,674 cm-1 up to pH 6.5. There was no change in the CC of the toxic TCA at 1640 cm-1 in ALDH at different pHs. The conclusion of the study was that the CHO of the toxic TCA was intercepted by the Oxy-Res under the action of ALDH in the mitochondria, particularly at pH 7.8. © 2019 IUBMB Life, 2019.


Subject(s)
Aldehyde Dehydrogenase/metabolism , Aldehydes/toxicity , Antineoplastic Agents/pharmacology , Mitochondria/drug effects , Plant Extracts/pharmacology , Stilbenes/pharmacology , Aldehydes/chemistry , Animals , Antineoplastic Agents/chemistry , Mitochondria/metabolism , Models, Molecular , Molecular Structure , Plant Extracts/chemistry , Rats , Stilbenes/chemistry
13.
Environ Toxicol Pharmacol ; 66: 14-23, 2019 Feb.
Article in English | MEDLINE | ID: mdl-30593950

ABSTRACT

Both ß-amyloid (Aß) catabolism and epigenetic regulation play critical roles in the onset of neurodegeneration. The latter also contribute to Pb neurotoxicity. The present study explored the role of epigenetic modifiers and Aß degradation enzymes in Pb-induced latent effects on Aß overproduction in vitro. Our results indicated that in SH-SY5Y cells exposed to Pb, the expression of NEP and IDE remained declined during the recovery period, accompanied with abnormal increase of Aß1-42 and amyloid oligomer. A disruption of selective global post-translational histone modifiers including the decrease of H3K9ac and H4K12ac and the induction of H3K9me2 and H3K27me2 dose dependently was also showed in recovery cells. Moreover, histone deacetylase inhibitor VPA could attenuate latent Aß accumulation and HDAC activity induced by Pb, which might be by regulating the expression of NEP and IDE epigenetically. Overall, our results suggest sustained reduction of NEP and IDE expression in response to Pb sensitizes recovery SH-SY5Y cells to Aß accumulation; however, administration of VPA is demonstrated to be beneficial in modulating Aß clearance.


Subject(s)
Amyloid beta-Peptides/metabolism , Lead/toxicity , Cell Line , Epigenesis, Genetic , Histone Deacetylase Inhibitors/pharmacology , Histones/metabolism , Humans , Insulysin/genetics , Insulysin/metabolism , Neprilysin/genetics , Neprilysin/metabolism , Tretinoin/pharmacology , Valproic Acid/pharmacology
14.
Spectrochim Acta A Mol Biomol Spectrosc ; 205: 568-573, 2018 Dec 05.
Article in English | MEDLINE | ID: mdl-30075437

ABSTRACT

The purposes of this study were to probe spectral behaviors of the toxic targets in trans-crotonaldehyde (TCA) in mitochondria altered by diallyl disulfides (DADS) derived from garlic. Ultraviolet absorption spectra showed that when ethanol as a solvent, the DADS blue shifted the peak of TCA from 318 nm to 312 nm. In mitochondria, the DADS further blue displaced the peak of TCA from 312 nm to 308 nm. Raman spectra displayed that the SS of DADS directly interacted with the CC toxic target of TCA, then the CCC of DADS interacted with the CHO toxic target of TCA. When DADS to TCA was 1:2, the DADS was the most powerful for the removal of the CC and CHO toxic targets of TCA. Study suggested that the SS of DADS altered the CC toxic target of TCA, while the CCC of DADS eliminated the CHO toxic target of TCA via local electron delocalization. The above two together clearly depicted the spectral behaviors of the toxic targets of TCA in mitochondria altered by DADS. These results are of great significance and value to elucidate the effects of garlic organic polysulfide on myocardial ischemia for the extensive development and use of garlic extracts.


Subject(s)
Aldehydes/analysis , Allyl Compounds/pharmacology , Cardiovascular Agents/pharmacology , Disulfides/pharmacology , Mitochondria/drug effects , Allyl Compounds/chemistry , Allyl Compounds/pharmacokinetics , Animals , Cardiovascular Agents/chemistry , Cardiovascular Agents/pharmacokinetics , Cells, Cultured , Disulfides/chemistry , Disulfides/pharmacokinetics , Male , Mitochondria/chemistry , Mitochondria/metabolism , Myocardial Ischemia , Myocardium/cytology , Rats , Rats, Wistar
15.
Int J Mol Med ; 41(6): 3195-3202, 2018 Jun.
Article in English | MEDLINE | ID: mdl-29512691

ABSTRACT

Lithocholic acid (LCA) is known to kill glioma cells while sparing normal neuronal cells. However, the anti-glioma mechanism of LCA is unclear at present. Although malondialdehyde (MDA) is not specific to detect tumors, biologically active α,ß-unsaturated aldehydes can be used to detect the outcome of gliomas, especially the mitochondria, as a research tool. The purpose of this research was to determine the optimum conditions for a lipid peroxidation model, according to changes in the aldehydes formed from the reaction between 2-thiobarbituric acid and biologically active α,ß-unsaturated aldehydes. Experimental methods and procedures were successfully established for a model of lipid peroxidation induced by H2O2 in glioma mitochondria for glioma treatment and optimum conditions for LCA treatment were determined. The optimal conditions for the model were a glioma mitochondrial concentration of 1.5 mg/ml, H2O2 concentration of 0.3 mg/ml, duration of action of 30 min, and addition of 4.0 ml of 46 mM thiobarbituric acid. The effect of LCA, as determined by changes in the UV peaks at 450, 495, and 532 nm, was optimal at a concentration of 100 µM, a duration of action of 15 min, and in an acidic microenvironment. The study concluded that a suitable concentration of LCA has anti-glioma effects as determined by the effect on changes in the UV peaks at 450, 495 and 532 nm and the mitochondrial model developed should be conducive to further in-depth research.


Subject(s)
Glioma/drug therapy , Hydrogen Peroxide/pharmacology , Lipid Peroxidation/drug effects , Lithocholic Acid/therapeutic use , Aldehydes/metabolism , Glioma/metabolism , Humans , In Vitro Techniques , Malondialdehyde/metabolism , Mitochondria/drug effects , Mitochondria/metabolism , Thiobarbiturates/metabolism
16.
Sci Rep ; 7(1): 10081, 2017 08 30.
Article in English | MEDLINE | ID: mdl-28855539

ABSTRACT

Inappropriate use of ethanol (EtOH) had led to noticeable health problems, but a beneficial phenomenon was found that EtOH displayed unique influences for toxic trans-crotonaldehyde (TCA) derived from mitochondrial lipid peroxidation. The influences of EtOH on the structure of TCA were systematically probed by UV-vis & Raman spectroscopy in the absence and presence of mitochondria, respectively. The maximum UV-vis peak at 301 nm of TCA was red shifted by hydroxyl (-OH) and methyl (-CH3) of EtOH, respectively. Raman stretching band of aldehyde (-CH=O) of TCA (TCA-CH=O) was split by the -CH3 of EtOH. The -CH3 increased TCA-CH=O stretching frequency while the -OH induced it. The more exposed -OH, the less stretching frequency. The ectopic -CH3 red shifted the UV-vis peak at 301 nm and Raman band of TCA-CH=O. In mitochondria, EtOH red shifted Raman stretching band of TCA-CH=O. Raman stretching bands of C-H, C-O and C-C of EtOH were red shifted, while Raman stretching bands of -CH2 and C-C-O of EtOH disappeared. The paper unearths the influences of EtOH to trap and transform the structure of TCA-CH=O. This discovery has an important contribution to eliminate TCA in order to protect and repair mtDNA by means of the decrease of 8-oxoG.


Subject(s)
Aldehydes/antagonists & inhibitors , Ethanol/chemistry , Mitochondria, Heart/chemistry , Aldehydes/toxicity , Animals , DNA, Mitochondrial/chemistry , DNA, Mitochondrial/drug effects , Ethanol/pharmacology , Guanine/analogs & derivatives , Guanine/antagonists & inhibitors , Guanine/chemistry , Lipid Peroxidation , Male , Mitochondria, Heart/drug effects , Mitochondria, Heart/metabolism , Myocardium/chemistry , Rats , Rats, Wistar , Spectrum Analysis, Raman
17.
IUBMB Life ; 69(7): 500-509, 2017 07.
Article in English | MEDLINE | ID: mdl-28419719

ABSTRACT

The purpose of this study is to probe the spectroscopic evidences of toxic trans-crotonaldehyde (TCA) trapped and transformed by resveratrol (Res) to prevent the damage of mitochondrial DNA. In aldehyde dehydrogenase (ALDH) at the different pH or mitochondria, the spectroscopic characteristics of TCA trapped and transformed by Res were observed by means of both UV-vis and Raman spectra. When Res interacted with TCA, TCA peak at 316 nm immediately disappeared while Res main peak at 305 nm and shoulder peak at 320 nm were dramatically changed, Raman peaks of TCA at 1,688 cm-1 assigned to CHO and 1,641 cm-1 affiliated to CC were strikingly shifted, Raman peaks of Res itself were significantly displaced or disappeared, especially in mitochondria and ALDH at different pH. The active groups of Res were the OH at C5 and C10 . The results of theoretical calculations are in agreement on the whole with the experimental data. The Res plays undoubtedly an important role via the structural change in TCA. The toxic CHO of TCA was effectively trapped and transformed by Res by means of itself OH at C5 and C10 . The mitochondrial alkaline microenvironment and ALDH promoted the elimination of toxic TCA. © 2017 IUBMB Life, 69(7):500-509, 2017.


Subject(s)
Aldehydes/chemistry , DNA, Mitochondrial/chemistry , Stilbenes/chemistry , Aldehyde Dehydrogenase/chemistry , Aldehydes/metabolism , Animals , DNA Damage , DNA, Mitochondrial/metabolism , Hydrogen-Ion Concentration , Male , Mitochondria/chemistry , Mitochondria/drug effects , Models, Molecular , Rats, Wistar , Resveratrol , Spectrophotometry, Ultraviolet , Spectrum Analysis, Raman , Stilbenes/pharmacology
18.
PLoS One ; 10(9): e0138073, 2015.
Article in English | MEDLINE | ID: mdl-26406606

ABSTRACT

Head splitting resistance (HSR) in cabbage is an important trait closely related to both quality and yield of head. However, the genetic control of this trait remains unclear. In this study, a doubled haploid (DH) population derived from an intra-cross between head splitting-susceptible inbred cabbage line 79-156 and resistant line 96-100 was obtained and used to analyze inheritance and detect quantitative trait loci (QTLs) for HSR using a mixed major gene/polygene inheritance analysis and QTL mapping. HSR can be attributed to additive-epistatic effects of three major gene pairs combined with those of polygenes. Negative and significant correlations were also detected between head Hsr and head vertical diameter (Hvd), head transverse diameter (Htd) and head weight (Hw). Using the DH population, a genetic map was constructed with simple sequence repeat (SSR) and insertion-deletion (InDel) markers, with a total length of 1065.9 cM and average interval length of 4.4 cM between adjacent markers. Nine QTLs for HSR were located on chromosomes C3, C4, C7, and C9 based on 2 years of phenotypic data using both multiple-QTL mapping and inclusive composite interval mapping. The identified QTLs collectively explained 39.4 to 59.1% of phenotypic variation. Three major QTLs (Hsr 3.2, 4.2, 9.2) showing a relatively larger effect were robustly detected in different years or with different mapping methods. The HSR trait was shown to have complex genetic mechanisms. Results from QTL mapping and classical genetic analysis were consistent. The QTLs obtained in this study should be useful for molecular marker-assisted selection in cabbage breeding and provide a foundation for further research on HSR genetic regulation.


Subject(s)
Brassica/genetics , Chromosomes, Plant/genetics , Genome, Plant , INDEL Mutation , Quantitative Trait Loci , Genome-Wide Association Study , High-Throughput Nucleotide Sequencing , Plant Breeding
19.
Front Plant Sci ; 6: 1118, 2015.
Article in English | MEDLINE | ID: mdl-26734028

ABSTRACT

Chromosome doubling of microspore-derived plants is an important factor in the practical application of microspore culture technology because breeding programs require a large number of genetically stable, homozygous doubled haploid plants with a high level of fertility. In the present paper, 29 populations of microspore-derived plantlets from cabbage (Brassica oleracea var. capitata) and broccoli (Brassica oleracea var. italica) were used to study the ploidy level and spontaneous chromosome doubling of these populations, the artificial chromosome doubling induced by colchicine, and the influence of tissue culture duration on the chromosomal ploidy of the microspore-derived regenerants. Spontaneous chromosome doubling occurred randomly and was genotype dependent. In the plant populations derived from microspores, there were haploids, diploids, and even a low frequency of polyploids and mixed-ploidy plantlets. The total spontaneous doubling in the 14 cabbage populations ranged from 0 to 76.9%, compared with 52.2 to 100% in the 15 broccoli populations. To improve the rate of chromosome doubling, an efficient and reliable artificial chromosome doubling protocol (i.e., the immersion of haploid plantlet roots in a colchicine solution) was developed for cabbage and broccoli microspore-derived haploids. The optimal chromosome doubling of the haploids was obtained with a solution of 0.2% colchicine for 9-12 h or 0.4% colchicine for 3-9 h for cabbage and 0.05% colchicine for 6-12 h for broccoli. This protocol produced chromosome doubling in over 50% of the haploid genotypes for most of the populations derived from cabbage and broccoli. Notably, after 1 or more years in tissue culture, the chromosomes of the haploids were doubled, and most of the haploids turned into doubled haploid or mixed-ploidy plants. This is the first report indicating that tissue culture duration can change the chromosomal ploidy of microspore-derived regenerants.

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