Pluteus insidiosus Complex, Four New Species Described and Pluteus reisneri Resurrected.

We studied the taxonomy of Pluteus insidiosus and similar species using morphological and molecular (nrITS, TEF1-α) data, including a detailed study of the type collection of P. insidiosus. Based on our results, we recognize five species in this group: P. insidiosus sensu stricto and four other taxa: P. assimilatus; P. farensis; P. flavostipitatus; and P. pseudoinsidiosus; described here as new. All these taxa are distinct from each other based on molecular data, but some of them are semi-cryptic based on morphology and co-occur in the Palaearctic region. An additional molecular lineage, phylogenetically separates from the P. insidiosus complex, but with many morphological similarities, was recognized in the molecular phylogenies. Based on the revision of available type collections, the name Pluteus reisneri Velen., was adopted for this Clade. Pluteus reisneri was validly published in 1921, but it has barely been used since its original description. A modern epitype, with molecular data, was selected for P. reisneri.

Volvariella turcica, a new species from Turkey, and a multigene phylogeny of Volvariella.

A new species of Volvariella, collected from Aydin Province on the coast of the Aegean Sea in southwestern Turkey, is described as Volvariella turcica, sp. nov., based on morphology and multigene molecular analysis of three nuc rDNA gene regions: internal transcribed spacer ITS1-5.8S-ITS2 (ITS), 28S, and 18S. The new species was found in forests dominated by Pinus brutia and Quercus coccifera and mainly characterized by small basidiomata with a white pileus covered with pale ochre center and an ochre-discoloring volva, small basidiospores, lageniform pleurocystidia, balloon-shaped to clavate cheilocystidia, and stipitipellis hairs that are cylindrical or cylindrical-tortuous with subcapitate or lobe-like projections. A comprehensive description, illustrations, and line drawings are provided, and comparison with morphologically similar and phylogenetically related species is discussed.

Evaluation of Bioactivities, Phenolic and Metal Content of Ten Wild Edible Mushrooms from Western Black Sea Region of Turkey.

In this study, we investigated the phenolic profile, metal concentrations, and antioxidant and antimicrobial activities of edible mushrooms collected from Sinop, Turkey: Amanita caesarea, Boletus edulis, Grifola frondosa, Hydnum repandum, Lactarius deliciosus, L. piperatus, L. volemus, Laetiporus sulphureus, Pleurotus ostreatus, and Ramaria flava. The mycochemical contents of R. flava, L. sulphureus, A. caesarea, L. deliciosus, and B. edulis were high. The cobalt (Co), cadmium (Cd), nickel (Ni), and lead (Pb) contents of mushrooms were between < 0.54 and 8.97 ppm. L. deliciosus had effective total antioxidant activity (7990 µmol α-tocopherol eq./g), ABTS·+ (2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid)) scavenging activity scavenging activity (EC50:7.87 µg/mL), and free-radical scavenging activity (EC50: 0.018 µg/mL) due to high levels of phenol, flavonoid, ß-carotene, and lycopene. B. edulis demonstrated strong reducing power (A0.5: 11.89 µg/mL), inhibition of linoleic acid peroxidation (EC50:0.0016 µg/mL), and H2O2 scavenging activity (EC50: 0.28 µg/mL). A. caesarea and R. flava showed the best metal chelating activity (EC50:44.31 µg/mL) and superoxide anion scavenging activity (EC50:0.18 µg/mL), respectively. Inhibition zone values of A. caesarea extract were detected between 8.1 and 27.1 mm for B. cereus. Our results show that mushrooms are promising dietary sources for natural prevention of many infectious diseases and that they act as antioxidant agents.

Interpolated mapping and investigation of environmental radioactivity levels in soils and mushrooms in the Middle Black Sea Region of Turkey.

The activity concentration of natural (238U, 232Th, and 40K) and artificial (137Cs) radionuclides was determined in 50 samples (obtained from the same station) from various species of mushrooms and soil collected from the Middle Black Sea Region (Turkey). The activities of 238U, 232Th, 40K, and 137Cs were found as 84 ± 16, 45 ± 14, 570 ± 28, and 64 ± 6 Bq kg-1 (dry weight), respectively, in the mushroom samples and as 51 ± 6, 41 ± 6, 201 ± 11, and 44 ± 4 Bq kg-1, respectively, in the soil samples for the entire area of study. The results of all radionuclide activity measurements, except those of 238U and 232Th in the mushroom samples, are consistent with previous studies. In the soil samples, the mean values of 238U and 232Th are above the world mean, and the activity mean of 40K is below the world mean. Finally, the activity estimation was made with both the soil and mushroom samples for unmeasured points within the study area by using the ordinary kriging method. Radiological distribution maps were generated.

Screening of antioxidant, antimicrobial activities and chemical contents of edible mushrooms wildly grown in the black sea region of Turkey.

The antioxidative activity of the methanol extracts obtained from twelve commonly consumed wild edible mushrooms was investigated according to the phosphomolybdenum method, inhibition of linoleic acid peroxidation, reducing power, metal chelating, O(2)(˙-), DPPH(˙), peroxide and H(2)O(2) scavenging activity in the Black Sea Region of Turky, and compared to standard antioxidant compounds such as a-tocopherol, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), and trolox. Total phenolic compounds, flavonoids, anthocyanins, ascorbic acid, ß-carotene and lycopene in the extracts were measured. The antimicrobial activities of extracts were also evalated against gram (-) and (+) bacteria and yeast with disc diffusion method. Hydnum repandum, Lactarius delicious, Lactarius camphoratus, Lactarius piperatus, Ramaria flava, and Agaricus bisporus were greater than α-tocopherol and BHT on antioxidant activity. The lipid peroxidation of L. camphoratus and Lactarius volemus was excellent, and was 58.05% and 61.44% respectively. The DPPH(˙) scavenging effects decreased in the order of R. flava>BHT> Macrolepiota procera>L. camphoratus>L. piperatus>Lactarius volemus>a-tocopherol and were, at 500 µg/ml and were 80.38, 76.61, 69.74, 61.55, 61.47, 61.09, 60.65%, respectively. All the extracts of mushroom also had the stronger chelating effect according to standards. The O(2)(˙-) scavenging effect of extracts of the mushroom species was in order of R. flava>Boletus edulis>Leatiporus sulphureus>M. procera>standards. L. piperatus, L. camphorates, L. volemus, A. bisporus, Chanterellus cibarius, L. sulphureus, H. repandum showed strong antimicrobial activity, especially on E. coli. H. repandum exhibited to be more effective on P. aeruginosa than other bacterial strains.

Antioxidant activities of Sarcodon imbricatum wildly grown in the Black Sea Region of Turkey.

The antioxidant activities of the methanol extract of Sarcodon imbricatum wildly grown in the Black Sea Region of Turkey were investigated in this study. Antioxidant activities were evaluated in terms of total antioxidant activity, reducing power, metal chelating ability, inhibition of linoleic acid peroxidation, superoxide, peroxide and hydrogen peroxide scavenging effects. Various antioxidant activities were compared to references antioxidants such as alpha-tocopherol, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), and trolox. In total antioxidant (12674.45 mumol alpha-tocopherol/g of extract), superoxide scavenging (53.74%) and peroxide scavenging activity (45.73%), the methanol extract of Sarcodon imbricatum showed stronger activity patterns than that of references antioxidants. Reducing power, metal chelating activity and free radical (DPPH(*)) scavenging activity was increased with the increasing concentration. The contents of total phenolic, flavonoid, anthocyanin, ascorbic acid, beta-carotene and lycopene of Sarcodon imbricatum were determined and found to be noteworthy.

Antioxidant activity of two wild edible mushrooms (Morchella vulgaris and Morchella esculanta) from North Turkey.

The ethanol extracts of Morchella vulgaris (EEMV) and Morchella esculanta (EEME) were analysed for their antioxidant activities in different systems including reducing power, free radical scavenging, superoxide anion radical scavenging, total antioxidant activity, and metal chelating activity. EEMV and EEME had similar reducing power, free radical scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging, and metal chelating activity at concentrations of 50, 100, and 150 microg/mL. These various antioxidant activities were compared to standard antioxidants such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), and alpha-tocopherol. The percent inhibition of different concentrations of EEMV on peroxidation in the linoleic acid system was 85 and 87 % respectively, which was greater than that of 100 and 250 microg/mL of alpha-tocopherol (50 and 77%, respectively) and similar to 250 microg/mL of BHA (85, 87%, respectively). The percent inhibition of different concentrations of EEME on peroxidation in the linoleic acid system was 80 and 87 % respectively, which was greater than that of 100 and 250 microg/mL of alpha-tocopherol (50, 77%) and similar to 250 microg/mL BHA (87%). On the other hand, the percent inhibition of 100 and 250 microg/mL of BHT was 97 and 99%, respectively. In addition, the total phenolic compounds in EEMV and EEME were determined as gallic acid equivalents.

REMOVED: Antioxidant activity of two wild edible mushrooms Morchella vulgaris and Morchella esculenta from north Turkey.

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Biosorption of heavy metals on Aspergillus fumigatus immobilized Diaion HP-2MG resin for their atomic absorption spectrometric determinations.

A solid phase extraction procedure based on biosorption of copper(II), lead(II), zinc(II), iron(III), nickel(II) and cobalt(II) ions on Aspergillus fumigatus immobilized Diaion HP-2MG has been investigated. The analytical conditions including amounts of A. fumigatus, eluent type, flow rates of sample and eluent solutions were examined. Good recoveries were obtained to the spiked natural waters. The influences of the concomitant ions on the retentions of the analytes were also examined. The detection limits (3sigma, N=11) were 0.30mugl(-1) for copper, 0.32mugl(-1) for iron, 0.41mugl(-1) for zinc, 0.52mugl(-1) for lead, 0.59mugl(-1) for nickel and 0.72mugl(-1) for cobalt. The relative standard deviations of the procedure were below 7%. The validation of the presented procedure is performed by the analysis of three standard reference materials (NRCC-SLRS 4 Riverine Water, SRM 1515 Apple leaves and GBW 07605 Tea). The procedure was successfully applied for the determination of analyte ions in natural waters microwave digested samples including street dust, tomato paste, black tea, etc.