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INTRODUCTION: Chest-physiotherapy is a key element in treatment of cystic fibrosis and patient adherence is a major issue in global cystic fibrosis care. This study aims to assess adherence to chest physiotherapy in adults with cystic fibrosis who not treated with tritherapy and to analyze the impact of certain factors on adherence. METHODS: Thus is a cross-sectional study, conducted using a questionnaire and a physiotherapy evaluation. Adherence to this treatment was measured in terms of quantitative and qualitative aspects. The impact on adherence of 15 factors was then assessed. RESULTS: Only 47% of patients could be considered as adherent, with a significant disparity between a quantitative and qualitative assessment. Gender, working time, pathology severity, the fact of being regularly followed by a physiotherapist, the perceived benefit of the sessions and their replacement by physical activity, seem associated with adherence to this treatment. CONCLUSIONS: Taking into account the qualitative aspect of the sessions, our study reveals a low rate, in our cohort, of adherence to respiratory physiotherapy, and highlights six factors likely to predominate. Regular follow-up by a physiotherapist seems to be a determining factor in adherence to this treatment.
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Deploying disease-resistant cultivars is one of the most effective control strategies to manage crop diseases such as wheat leaf rust, caused by Puccinia triticina. After harvest, this biotrophic fungal pathogen can survive on wheat volunteers present at landscape scale and constitute a local source of primary inoculum for the next cropping season. In this study, we characterised the diversity of P. triticina populations surveyed on wheat volunteer seedlings for six consecutive years (2007-2012) at the landscape scale. A total of 642 leaf rust samples classified in 52 virulence profiles (pathotypes) were collected within a fixed 5-km radius. The pathotype composition (identity and abundance) of field-collected populations was analyzed according to the distance between the surveyed wheat plots and to the cultivars of origin of isolates. Our study emphasised the high diversity of P. triticina populations on wheat volunteers at the landscape scale. We observed an impact of cultivar of origin on pathogen population composition. Levels of population diversity differed between cultivars and their deployment in the study area. Our results suggest that wheat volunteers could provide a significant though highly variable contribution to the composition of primary inoculum and subsequent initiation of leaf rust epidemics.
Subject(s)
Basidiomycota , Triticum , Humans , Triticum/microbiology , Plant Diseases/microbiology , PucciniaABSTRACT
The assessment of waste ecotoxicity (hazardous property HP14 in the European Union) is fundamental for proper waste classification and safe application/disposal. Biotests are relevant for evaluating waste complex matrices, but their efficiency is crucial to encourage their adoption at the industrial level. This work aims at evaluating possibilities of improving the efficiency of a biotest battery previously suggested in the literature, regarding test selection, duration, and/or laboratory resources optimization. Fresh incineration bottom ash (IBA) was the case study. The test battery analysed included standard aquatic (bacteria, microalgae, macrophytes, daphnids, rotifers, fairy shrimp) and terrestrial (bacteria, plants, earthworms, collembolans) organisms. The assessment followed an Extended Limit Test design (three dilutions of eluate or solid IBA) and the Lowest Ineffective Dilution (LID-approach) for ecotoxicity classification. The results emphasize the importance of testing different species. It was also evidenced that tests with daphnids and earthworms may be shortened to 24 h; the miniaturization of tests is suitable as e.g. differential sensitivity of microalgae and macrophytes was captured with low variability; alternative testing kits can be used when methodological difficulties are found. Microalgae were more sensitive than macrophytes. Similar results were found for the Thamnotoxkit and daphnids test for eluates with natural pH, so the former may be used as an alternative. B. rapa was the most sensitive organism, suggesting that it may be tested as the only terrestrial plant species and that minimum test duration is appropriate. F. candida does not appear to add information to the battery. The differences in sensitivity of A. fischeri and E. fetida compared to the remaining species were not significant enough to exclude them from the battery. Thus, this work suggests a biotest battery to test IBA comprising aquatic tests - Aliivibrio fischeri, Raphidocelis subcapitata (miniaturised test), and Daphnia magna (24 h when clear deleterious effects are observed) or Thamnocephalus platyurus (toxkit) - and terrestrial tests - Arthrobacter globiformis, Brassica rapa (14 d), and Eisenia fetida (24 h). Testing waste with natural pH is also recommended. The Extended Limit Test design considering the LID-approach seems useful in waste testing, particularly for the industry, involving low effort, test material requirements, and few laboratory resources. The LID-approach allowed for differentiating ecotoxic from non-ecotoxic effects and captured different sensitivities between species. Ecotoxicological assessment of other waste may benefit from these recommendations, but caution should be taken given the properties of each waste type.
Subject(s)
Coal Ash , Incineration , European Union , PlantsABSTRACT
Hyaluronic acid (HA) and gelatin (Gel) are major components of the extracellular matrix of different tissues, and thus are largely appealing for the construction of hybrid hydrogels to combine the favorable characteristics of each biopolymer, such as the gel adhesiveness of Gel and the better mechanical strength of HA, respectively. However, despite previous studies conducted so far, the relationship between composition and scaffold structure and physico-chemical properties has not been completely and systematically established. In this work, pure and hybrid hydrogels of methacroyl-modified HA (HAMA) and Gel (GelMA) were prepared by UV photopolymerization and an extensive characterization was done to elucidate such correlations. Methacrylation degrees of ca. 40% and 11% for GelMA and HAMA, respectively, were obtained, which allows to improve the hydrogels' mechanical properties. Hybrid GelMA/HAMA hydrogels were stiffer, with elastic modulus up to ca. 30 kPa, and porous (up to 91%) compared with pure GelMA ones at similar GelMA concentrations thanks to the interaction between HAMA and GelMA chains in the polymeric matrix. The progressive presence of HAMA gave rise to scaffolds with more disorganized, stiffer, and less porous structures owing to the net increase of mass in the hydrogel compositions. HAMA also made hybrid hydrogels more swellable and resistant to collagenase biodegradation. Hence, the suitable choice of polymeric composition allows to regulate the hydrogels´ physical properties to look for the most optimal characteristics required for the intended tissue engineering application.
Subject(s)
Biocompatible Materials/chemistry , Gelatin/chemistry , Hyaluronic Acid/chemistry , Hydrogels/chemistry , Methacrylates/chemistry , Tissue Engineering/methods , Tissue Scaffolds/chemistry , Humans , Polymers/chemistryABSTRACT
INTRODUCTION: Hypofractionated radiation therapy for breast cancer requires highly precise delivery through the use of image-guided radiotherapy (IGRT). Surface-guided radiation therapy (SGRT) is being increasingly used for patient positioning in breast radiotherapy. We aimed to assess the role of SGRT for verification of breast radiotherapy and the tumour bed. MATERIALS AND METHODS: Prospective study of 252 patients with early stage breast cancer. A total of 1170 determinations of daily positioning were performed. Breast surface positioning was determined with SGRT (AlignRT) and correlated with the surgical clips in the tumour bed, verified by IGRT (ExacTrac). RESULTS: SGRT improved surface matching by a mean of 5.3 points compared to conventional skin markers (98.0 vs. 92.7), a statistically significant difference (p < 0.01, Wilcoxon Test). For surface matching values > 95%, ≥ 3 clips coincided in 99.7% of the determinations and all markers coincided in 92.5%. For surface matching rates > 90%, the location of ≥ 3 clips coincided in 99.55% of determinations. CONCLUSIONS: SGRT improves patient positioning accuracy compared to skin markers. Optimal breast SGRT can accurately verify the localisation of the tumour bed, ensuring matching with ≥ 3 surgical clips. SGRT can eliminate unwanted radiation from IGRT verification systems.
Subject(s)
Breast Neoplasms/radiotherapy , Patient Positioning/methods , Radiotherapy, Image-Guided/methods , Adult , Aged , Aged, 80 and over , Breast/diagnostic imaging , Breast Neoplasms/diagnostic imaging , Breast Neoplasms/pathology , Breast Neoplasms/surgery , Female , Fiducial Markers , Humans , Mastectomy, Segmental , Middle Aged , Organ Sparing Treatments/methods , Prospective Studies , Radiation Dose Hypofractionation , Statistics, Nonparametric , Surgical Instruments , Tomography, X-Ray ComputedABSTRACT
OBJECTIVE: To define the sensitization pattern of patients with anaphylaxis to Vespa velutina nigrithorax (VVN). METHODS: We studied 100 consecutive Spanish patients with anaphylaxis to Hymenoptera venom and systematically determined specific IgE (sIgE) to whole venoms (Vespula species, Polistes dominula, Apis mellifera, Vespa crabro, and Dolichovespula maculata) and their molecular components (rApi m 1, rApi m 5, rApi m 10, rVes v 1, rVes v 5, rPol d 5, and cross-reactive carbohydrates). Specific IgE to VVN venom and its antigen 5 (nVesp v 5) were measured in a subsample. RESULTS: Seventy-seven patients had anaphylaxis to VVN. Of these, only 16 (20.8%) reported previous VVN stings, but were stung by other Hymenoptera. Positive sIgE (>0.35 kUA/L) to each of the whole venoms was detected in >70% of patients (Vespula species in 100%). The components showing >50% positivity were rApi m 5 (51.4%), rPol d 5 (80.0%), and rVes v 5 (98.7%). This pattern was similar to that of Vespula species anaphylaxis (n=11) but different from that of A mellifera anaphylaxis (n=10). Specific IgE to nVesp v 5 was positive in all patients (n=15) with VVN anaphylaxis and was correlated with sIgE to both rVes v 5 (R=0.931) and rPol d 5 (R=0.887). CONCLUSIONS: VVN has become the commonest cause of Hymenoptera anaphylaxis in our area. Most cases report no previous VVN stings. Their sensitization pattern is similar to that of patients with anaphylaxis to other Vespidae. Specific IgE to antigen-5 from VVN, Vespula species, and P dominula are strongly correlated in patients with VVN anaphylaxis.
Subject(s)
Allergens/immunology , Anaphylaxis/immunology , Hypersensitivity/immunology , Insect Bites and Stings/immunology , Wasp Venoms/immunology , Adult , Aged , Aged, 80 and over , Anaphylaxis/epidemiology , Animals , Female , Humans , Hypersensitivity/epidemiology , Immunization , Immunoglobulin E/metabolism , Insect Bites and Stings/epidemiology , Male , Middle Aged , Spain/epidemiology , Wasps , Young AdultABSTRACT
The knowledge of material properties and their behavior at high temperatures is of crucial importance in many fields. For instance, annealing phenomena occurring during the thermomechanical processing of materials, such as recrystallization, have long been recognized as being both of scientific interest and technological importance. Different methods are currently used to study annealing phenomena and submit metals to heat loads. In this work, we present the design and the development of a laser-based facility for annealing tests. This experimental setup enables studies at the laboratory scale with great flexibility to submit samples to various spatial and temporal heating profiles. Due to the possibility of having optical access to the sample, laser heating can be combined with several non-contact diagnostics such as infrared imaging to control and analyze the temperature gradients. As a case study, we present a set of experiments performed to study the recrystallization kinetics of tungsten. We demonstrate that samples can be heated linearly with heating rate up to â¼2000 K/s, at temperatures above 2000 K, for seconds or hours, with typical errors in the temperature measurement of around 1% that depend mainly on the determination of sample emissivity. Such studies are of crucial interest in the framework of nuclear fusion since the international thermonuclear experimental reactor nuclear reactor will operate with a full-W divertor.
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OBJECTIVES/BACKGROUND: Rapid eye movement (REM) Sleep Behavior Disorder (RBD) in Parkinson's disease (PD) may be associated with a malignant phenotype. Despite its prognostic value, little is known about the time course of RBD in PD. In this study, we aimed to ascertain whether or not RBD is a stable feature in PD. In this study, we prospectively evaluated clinical and neurophysiological features of RBD, including REM Sleep Without Atonia (RSWA), in PD patients with RBD at baseline and after three years then assessed whether the changes in measures of RSWA parallel the progression of PD. PATIENTS/METHODS: In sum, 22 (17M, mean age 64.0 ± 6.9 years) moderate-to-advanced PD patients (mean PD duration at baseline:7.6±4.8 years) with RBD, underwent a video-polysomnography (vPSG) recording and clinical and neuropsychological assessment at baseline and after three years. RESULTS: At follow-up, the self-assessed frequency of RBD symptoms increased in six patients, decreased in six and remained stable in 10, while RSWA measures significantly increased in all subjects. At follow-up, patients showed worse H&Y stage (p = 0.02), higher dopaminergic doses (p = 0.05) and they performed significantly worse in phonetic and semantic fluency tests (p = 0.02; p = 0.04). Changes in RSWA correlated significantly with the severity in levodopa-induced dyskinesia (r = 0.61,p = 0.05) and motor fluctuation (r = 0.54,p = 0.03) scores, and with the worsening of executive functions (r = 0.78,p = 0.001) and visuo-spatial perception (r = -0.57,p = 0.04). CONCLUSION: Despite the subjective improvement of RBD symptoms in one-fourth of PD patients, all RSWA measures increased significantly at follow-up, and their changes correlated with the clinical evolution of motor and non-motor symptoms. RBD is a long-lasting feature in PD and RSWA is a marker of the disease's progression.
Subject(s)
Parkinson Disease , REM Sleep Behavior Disorder , Aged , Humans , Levodopa , Middle Aged , Parkinson Disease/complications , Parkinson Disease/drug therapy , Polysomnography , REM Sleep Behavior Disorder/etiology , Sleep, REMABSTRACT
BACKGROUND AND AIMS: The dynamics of plant architecture is a central aspect of plant and crop models. Most models assume that whole shoot development is orchestrated by the leaf appearance rate, which follows a thermal time schedule. However, leaf appearance actually results from leaf extension and taking it as an input hampers our ability to understand shoot construction. The objective of the present study was to assess a modelling framework for grasses, in which the emergence of leaves and other organs is explicitly calculated as a result of their extension. METHODS: The approach builds on a previous model, which uses a set of rules co-ordinating the timing of development within and between phytomers. We first assessed rule validity for four experimental datasets, including different cultivars, planting densities and environments, and accordingly revised the equations driving the extension of the upper leaves and of internodes. We then fitted model parameters for each dataset and evaluated the ability to simulate the measured phenotypes across time. Finally, we carried out a sensitivity analysis to identify the parameters that had the greatest impact and to investigate model behaviour. KEY RESULTS: The modified version of the model simulated correctly the contrasting maize phenotypes. Co-ordination rules accounted for the observations in all studied cultivars. Factors with major impact on model output included extension rates, the time of tassel initiation and initial conditions. A large diversity of phenotypes could be simulated. CONCLUSIONS: This work provides direct experimental evidence for co-ordination rules and illustrates the capacity of the model to represent contrasting phenotypes. These rules play an important role in patterning shoot architecture and some of them need to be assessed further, considering contrasting growth conditions. To make the model more predictive, several parameters could be considered in the future as internal variables driven by plant status.
Subject(s)
Plant Leaves , Zea mays , Algorithms , PhenotypeSubject(s)
Porphyria, Acute Intermittent/complications , Posterior Leukoencephalopathy Syndrome/complications , Diagnosis, Differential , Female , Humans , Magnetic Resonance Imaging , Middle Aged , Porphyria, Acute Intermittent/diagnosis , Porphyria, Acute Intermittent/urine , Posterior Leukoencephalopathy Syndrome/diagnosis , Posterior Leukoencephalopathy Syndrome/urine , Recurrence , Seizures/diagnosis , Seizures/etiology , Seizures/urine , UrinalysisABSTRACT
GEMS is an IRAM 30m Large Program whose aim is determining the elemental depletions and the ionization fraction in a set of prototypical star-forming regions. This paper presents the first results from the prototypical dark cloud TMC 1. Extensive millimeter observations have been carried out with the IRAM 30m telescope (3 mm and 2 mm) and the 40m Yebes telescope (1.3 cm and 7 mm) to determine the fractional abundances of CO, HCO+, HCN, CS, SO, HCS+, and N2H+ in three cuts which intersect the dense filament at the well-known positions TMC 1-CP, TMC 1-NH3, and TMC 1-C, covering a visual extinction range from A V ~ 3 to ~20 mag. Two phases with differentiated chemistry can be distinguished: i) the translucent envelope with molecular hydrogen densities of 1-5×103 cm-3; and ii) the dense phase, located at A V > 10 mag, with molecular hydrogen densities >104 cm-3. Observations and modeling show that the gas phase abundances of C and O progressively decrease along the C+/C/CO transition zone (A V ~ 3 mag) where C/H ~ 8×10-5 and C/O~0.8-1, until the beginning of the dense phase at A V ~ 10 mag. This is consistent with the grain temperatures being below the CO evaporation temperature in this region. In the case of sulfur, a strong depletion should occur before the translucent phase where we estimate a S/H ~ (0.4 - 2.2) ×10-6, an abundance ~7-40 times lower than the solar value. A second strong depletion must be present during the formation of the thick icy mantles to achieve the values of S/H measured in the dense cold cores (S/H ~8×10-8). Based on our chemical modeling, we constrain the value of ζ H2 to ~ (0.5 - 1.8) ×10-16 s-1 in the translucent cloud.
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Freshwater ecosystems face widespread diffuse and point-source contamination. Species Sensitivity Distributions (SSDs) have been used as a tool to determine chemical concentration benchmarks that represent protective levels for most species in the environment. Here we used a SSD approach to assess on the adequacy of standard planktonic organisms to reflect the response of benthic communities, critically supporting the structure and function of lotic ecosystems. For the purpose, SSDs reflecting non-lethal responses of standard planktonic and selected benthic organisms were built based on EC50 values (collected in the literature or estimated following testing herein) regarding three model contaminants: potassium dichromate (PD), 3,5-dichlorophenol (DCP) and lead chloride (LC). The derived HC5 estimates were discriminatory between chemicals and the uncertainty associated with the estimate was remarkably low. The HC5 estimates with corresponding uncertainty were generally within the same order of magnitude for the three chemicals tested, with better discrimination between chemicals regarding their hazardous potential being achieved for benthic organisms: DCP was clearly less hazardous than PD, but LC tends to be as hazardous as PD and DCP (assuming the confidence interval ranges). Moreover, benthic communities were more sensitive to both DCP and PD, in this later case the HC5 being lower by more than one order of magnitude than that found for planktonic communities; for LC, confidence intervals overlapped, preventing a feasible assumption regarding differential sensitivity of the compared communities. Microphytobenthos was highlighted as the most sensitive group to the three tested chemicals in SSDs covering the benthic compartment, while SSDs with planktonic organisms did not consistently show trends in sensitivity ordering. Overall, our results suggest that protective benchmarks retrieved from SSDs built with the responses of standard planktonic organisms (which are the most commonly used for regulation purposes) do not adequately protect benthic communities.
Subject(s)
Benchmarking/methods , Fresh Water/chemistry , Oligochaeta/drug effects , Plankton/drug effects , Water Pollutants, Chemical/toxicity , Animals , Conservation of Natural Resources/methods , Ecosystem , Ecotoxicology , Risk Assessment , Species Specificity , Water Pollutants, Chemical/analysisABSTRACT
PURPOSE: To evaluate the effect of the optical zone diameter (OZ) in orthokeratology contact lenses regarding the topographical profile in patients with high myopia (-4.00 D to -7.00 D) and to study its effect over the visual quality. MATERIALS AND METHODS: Twelve patients (18 eyes) were fitted with overnight orthokeratology (OrthoK) with a randomized 6 mm or 5 mm OZ lens worn for 2 weeks, followed by a 2-week washout period, between both designs. Keratometry (K) readings, optical zone treatment diameter (OZT), peripheral ring width (PRW), higher-order aberrations (HOA), high (HC) and low contrast (LC) visual acuity, and subjective vision and comfort were measured at baseline and after 2 weeks of OrthoK lens wear of each contact lens. RESULTS: No significant differences were found between any measurements for the same subject at both baselines (p value > 0.05). There was no difference between OZ lens designs found in refraction, subjective vision or comfort, and HC and LC visual acuity. Contrast sensitivity was decreased in the 5 mm OZ lens design compared with 6 mm OZ design (p-value < 0.05). 5 mm OZ design provoked a greater flattening, more powerful midperipheral ring and 4th-order corneal and total spherical aberration than the 6 mm OZ design, being statistically significant after 7 days, for corneal aberration, and 15 days, for corneal and total, of wearing the lens (p-value < 0.05). The OZT obtained were 2.8 ± 0.2 mm and 3.1 ± 0.1 mm for 5 mm and 6 mm OZ design, respectively (p-value < 0.05). Regarding PRW, the 5 mm OZ design had a wider ring width in both the nasal and temporal zones (p-value < 0.05). CONCLUSIONS: A smaller diameter optical zone (5 mm) in orthokeratology lenses produces a smaller treatment area and a larger and more powerful midperipheral ring, increasing the 4th-order spherical aberration that affects only the contrast sensitivity but without differences in visual acuity and subjective vision compared with a larger OZ diameter (6 mm).
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Background and Aims: Growing cultivars differing by their disease resistance level together (cultivar mixtures) can reduce the propagation of diseases. Although architectural characteristics of cultivars are little considered in mixture design, they could have an effect on disease, in particular through spore dispersal by rain splash, which occurs over short distances. The objective of this work was to assess the impact of plant height of wheat cultivars in mixtures on splash dispersal of Zymoseptoria tritici, which causes septoria tritici leaf blotch. Methods: We used a modelling approach involving an explicit description of canopy architecture and splash dispersal processes. The dispersal model computed raindrop interception by a virtual canopy as well as the production, transport and interception of splash droplets carrying inoculum. We designed 3-D virtual canopies composed of susceptible and resistant plants, according to field measurements at the flowering stage. In numerical experiments, we tested different heights of virtual cultivars making up binary mixtures to assess the influence of this architectural trait on dispersal patterns of spore-carrying droplets. Key Results: Inoculum interception decreased exponentially with the height relative to the main inoculum source (lower diseased leaves of susceptible plants), and little inoculum was intercepted further than 40 cm above the inoculum source. Consequently, tall plants intercepted less inoculum than smaller ones. Plants with twice the standard height intercepted 33 % less inoculum than standard height plants. In cases when the height of suscpeptible plants was doubled, inoculum interception by resistant leaves was 40 % higher. This physical barrier to spore-carrying droplet trajectories reduced inoculum interception by tall susceptible plants and was modulated by plant height differences between cultivars of a binary mixture. Conclusions: These results suggest that mixture effects on spore dispersal could be modulated by an adequate choice of architectural characteristics of cultivars. In particular, even small differences in plant height could reduce spore dispersal.
Subject(s)
Disease Resistance , Plant Diseases/prevention & control , Triticum/anatomy & histology , Ascomycota , Flowers/anatomy & histology , Imaging, Three-Dimensional , Plant Diseases/etiology , Plant Leaves/anatomy & histology , Rain , Spores, Fungal , Triticum/immunology , Triticum/physiologyABSTRACT
The present report is of two patients who, immediately after internal carotid endarterectomy, presented with unexplained hemiplegia, despite normal findings on repeated MRI scans, which secondarily evolved into homolateral subacute corticobasal syndrome (CBS), with asymmetrical hemispheric hypometabolism and evidence of dopaminergic denervation. This prompted us to propose an hypothesis of transient cerebral hypoxia arising during the surgical clamping period that might have provoked a prolonged or permanent functional lesion of the left hemisphere and basal ganglia, with no visible infarction on MRI but only synaptic rearrangement of the neural networks, thereby revealing or exacerbating a potentially preexisting silent impairment.
Subject(s)
Basal Ganglia Diseases/etiology , Endarterectomy, Carotid/adverse effects , Postoperative Complications/therapy , Aged , Basal Ganglia Diseases/diagnostic imaging , Basal Ganglia Diseases/therapy , Cerebral Hemorrhage/etiology , Cerebral Hemorrhage/therapy , Cerebrovascular Circulation , Diffusion Magnetic Resonance Imaging , Dopamine Agents/therapeutic use , Hemiplegia/etiology , Hemiplegia/therapy , Humans , Hypoxia, Brain/etiology , Hypoxia, Brain/therapy , Levodopa/therapeutic use , Magnetic Resonance Imaging , Male , Memory Disorders/etiology , Memory Disorders/psychology , Neuropsychological Tests , Positron-Emission Tomography , Postoperative Complications/diagnostic imaging , Tomography, X-Ray ComputedSubject(s)
Anaphylaxis/immunology , Disaccharides/administration & dosage , Disaccharides/adverse effects , Drug Hypersensitivity/immunology , Administration, Intravaginal , Aged , Anaphylaxis/diagnosis , Anaphylaxis/drug therapy , Anti-Allergic Agents/therapeutic use , Antifungal Agents/administration & dosage , Capsules , Disaccharides/immunology , Drug Compounding , Drug Hypersensitivity/diagnosis , Drug Hypersensitivity/drug therapy , Epitopes , Female , Humans , Imidazoles/administration & dosage , Intradermal Tests , Treatment OutcomeABSTRACT
INTRODUCTION: One of the objectives of the French expert centers for Parkinson's disease (NS-Park) network was to determine a consensus procedure for assessing cognitive function in patients with Parkinson's. This article presents this procedure and briefly describes the selected tests. METHODS: A group of 13 experts used the Delphi method for consensus building to define the overall structure and components of the assessment procedure. For inclusion in the battery, tests had to be validated in the French language, require little motor participation, have normative data and be recognized by the international community. Experimental tasks and tests requiring specific devices were excluded. RESULTS: Two possibilities were identified, depending on whether an abbreviated or comprehensive assessment of cognitive function was necessary. For an abbreviated assessment, the experts recommended the Montreal Cognitive Assessment (MoCA) as a screening test for cognitive impairment or dementia. For a comprehensive neuropsychological assessment, the experts recommended assessing global efficiency plus the five main cognitive domains (attention and working memory, executive function, episodic memory, visuospatial function and language) that may be impaired in Parkinson's disease, using two tests for each domain. DISCUSSION AND CONCLUSION: A common procedure for assessing cognitive function is now available across the French network dedicated to Parkinson's disease, and is recommended for both research and clinical practice. It will also help to promote standardization of the neuropsychological assessment of Parkinson's disease.
Subject(s)
Cognition Disorders/diagnosis , Cognition/physiology , Neuropsychological Tests , Parkinson Disease/physiopathology , Parkinson Disease/psychology , Cognition Disorders/physiopathology , Cognition Disorders/psychology , Consensus , Delphi Technique , Executive Function , Expert Testimony , France , Humans , Memory, Short-Term , Neuropsychological Tests/standards , Parkinson Disease/diagnosisABSTRACT
O experimento foi conduzido em delineamento inteiramente ao acaso para avaliar os efeitos de diferentes níveis de glicina+serina (gli+ser) total em dietas de baixa proteína bruta (PB) sobre o desempenho e características de carcaça de frangos de corte de um a 21 dias de idade. Foram utilizadas 750 aves, distribuídas em cinco tratamentos, cinco repetições e 30 aves por unidade experimental. As dietas utilizadas continham 190g/kg de PB e níveis de gli+ser de 16,7; 19,2; 21,7 e 24,2g/kg; a dieta controle continha 230g/kg de PB. Avaliaram-se os efeitos dos níveis de glicina+serina sobre o desempenho e composição de carcaça. Os dados foram submetidos à análise de variância e os resultados obtidos com as dietas de baixos níveis proteicos com diferentes níveis de gli+ser foram comparados à dieta controle pelo teste de Dunnett. Os níveis de gli+ser apresentaram efeito linear sobre a conversão alimentar, ganho de peso e peso aos 21 dias, sendo o nível de 24,2g/kg de gli+ser com resultado semelhante à dieta controle. A redução proteica aumentou o conteúdo de extrato etéreo na carcaça.(AU)
A completely randomized experimental design was carried out to evaluate the effects of total gly+ser levels in low crude protein diets on performance and body composition of male broiler chickens from 1 to 21 days of age. Seven hundred and fifty broiler chickens were used in each one of the production phases. The birds were randomly allotted to five treatments and five replicates. The diets contained 190g/kg crude protein (CP) and total gly+ser levels of 16.7; 19.2; 21.7 and 24.2g/kg; and a control diet with 230 g/kg CP. The effect of glycine+serine levels on performance and body composition was evaluated. Data were subjected to analysis of variance and the results obtained with the diets of low crude protein levels with different levels of Gly + Ser were compared to the control diet by Dunnett's test. The gly+ser levels improved the feed linearly: weight gain ratio; weight gain and final weight. The broilers fed 24.2g/kg gly+ser diet showed a performance similar to broilers fed control diets. The carcass fat increased with CP reduction.(AU)
Subject(s)
Animals , Serine/administration & dosage , Dietary Proteins/administration & dosage , Weight Gain , Chickens , Glycine/administration & dosage , Animal FeedABSTRACT
Estudou-se o efeito do teor de metionina + cistina sobre o desempenho de codornas de corte durante a fase inicial (nascimento ao 21° dia). Foram utilizadas 360 codornas, de ambos os sexos, distribuídas em delineamento experimental inteiramente ao acaso. Os tratamentos consistiram em seis níveis de metionina + cistina total (0,86; 0,96; 1,06; 1,16; 1,26 e 1,36), quatro repetições e 15 aves por unidade experimental. As características avaliadas foram o peso corporal, ganho de peso, consumo e conversão alimentar para fase inicial. Observou-se efeito quadrático significativo do teor de metionina + cistina sobre todas as variáveis avaliadas do nascimento ao 21° dia de idade, sendo observado melhor desempenho nas codornas alimentadas com 1,21% de metionina + cistina para peso corporal e ganho de peso, 1,36% de metionina + cistina para consumo e 1,1% de metionina + cistina para conversão alimentar. A exigência de metionina + cistina para máximo ganho de peso na fase inicial é de 1,21%.
The effect of total methionine + cystine level on the performance traits of European quails during the initial phase of the growth period (from hatch to 21 days of age) was evaluated in a completely randomized experimental design with six levels of total methionine + cystine (.86; .96; 1.06; 1.16;1.26;1.36), with four replicates and 15 quails per experimental unit. Body weight, weight gain, feed intake and feed: weight gain ratio were evaluated. Significant quadratic effects of total methionine + cystine level on the performance traits recorded from hatch to 21 days of age were observed. Highest body weight and weight gain were estimated for quails fed 1.21% total methionoine + cystine diets, lowest feed intake was estimated for quails fed 1.36% diets and best feed: weight gain ratio was estimated for quails fed 1.1% total methionoine + cystine diet. The total methionine + cystine requirement for weight gain from hatch to 21 days of age is 1.21%.
Subject(s)
Animals , Infant, Newborn , Coturnix/metabolism , Methionine/administration & dosage , Methionine/adverse effectsABSTRACT
OBJECTIVE: To assess the frequency of symptoms of impulse control disorders (ICD, namely pathological gambling, compulsive sexual behaviour, compulsive eating and compulsive shopping) and related behaviours (hobbyism, punding, walkabout and dopamine dysregulation syndrome) in patients with Parkinson's disease (PD) with and without probable rapid eye movement, sleep behaviour disorder (pRBD). METHODS: Two hundred and sixteen consecutive PD patients, attending two university-based movement disorders clinics, were screened for p-RBD using the RBD Single Question and the RBD Screening Questionnaire (RBDSQ). Current ICDs and related behaviours symptoms were assessed with the Questionnaire for Impulsive-Compulsive Disorders in PD (QUIP)-short form. RESULTS: PD-pRBD patients (n=106/216;49%) had a longer PD duration, a higher Hoehn & Yahr score, a greater levodopa-equivalent daily dose (LEDD), but no difference in dopamine agonist use, compared to PD-without pRBD. A higher proportion of one or more current ICDs and related behaviours symptoms was reported in PD-pRBD compared to PD-without RBD (53% vs28%; p=0.0002). In a multivariate regression analysis accounting for gender, age of onset, PD duration, PD severity, depression score and total and dopaminergic agonist-LEDD, RBD was associated to a relative risk of 1.84 for any ICD or related behaviours symptoms (p=0.01), and to a risk of 2.59 for any ICD symptoms only (p=0.001). Furthermore, PD-pRBD had a more than fourfold risk for symptoms of pathological gambling (relative risk (RR): 4.87; p=0.049) compared to PD-without pRBD. CONCLUSIONS: The present study indicates that RBD is associated with an increased risk of developing symptoms of ICDs in PD. Identifying RBD in PD may help clinicians to choose the best therapeutic strategy. TRIAL REGISTRATION: AU1023 Institutional Ethics Committee.