Search | Brasil - Virtual Health Library

A refocused and optimized HNCA: increased sensitivity and resolution in large macromolecules.

Farmer, B T; Venters, R A; Spicer, L D; Wittekind, M G; Müller, L.

J Biomol NMR ; 2(2): 195-202, 1992 Mar.

Article in English | MEDLINE | ID: mdl-1422152

ABSTRACT

A 3D optimized, refocused HNCA experiment is described. It is demonstrated to yield a dramatic increase in sensitivity when applied to [13C, 15N]-labeled human carbonic anhydrase II, a 29-kDa protein. The reasons for the gain in sensitivity are discussed, and 3 distinct areas for further development are indicated.

Subject(s)

Carbonic Anhydrases/chemistry , Isoenzymes/chemistry , Magnetic Resonance Spectroscopy/methods , Carbon Isotopes , Humans , Mathematics , Nitrogen Isotopes , Protein Conformation

The structure of HPr and site-directed mutagenesis.

Waygood, E B; Sharma, S; Bhanot, P; el-Kabbani, O A; Delbaere, L T; Georges, F; Wittekind, M G; Klevit, R E.

FEMS Microbiol Rev ; 5(1-2): 43-52, 1989 Jun.

Article in English | MEDLINE | ID: mdl-2483820

Subject(s)

Mutation , Phosphoenolpyruvate Sugar Phosphotransferase System , Adenosine Triphosphate/metabolism , Amino Acid Sequence , Bacterial Proteins/genetics , Bacterial Proteins/immunology , Bacterial Proteins/metabolism , Epitopes/analysis , Glutamates/metabolism , Molecular Sequence Data , Molecular Structure , Phosphoenolpyruvate Sugar Phosphotransferase System/genetics , Phosphoenolpyruvate Sugar Phosphotransferase System/immunology , Phosphoenolpyruvate Sugar Phosphotransferase System/metabolism , Phosphorylation , Protein Conformation

ABSTRACT

Subject(s)

Subject(s)

SEND TO:

SELECTION OF CITATIONS

SEARCH DETAIL