Epidemic Characteristics and Spatio-Temporal Patterns of HFRS in Qingdao City, China, 2010-2022.

Objective: This study investigated the epidemic characteristics and spatio-temporal dynamics of hemorrhagic fever with renal syndrome (HFRS) in Qingdao City, China. Methods: Information was collected on HFRS cases in Qingdao City from 2010 to 2022. Descriptive epidemiologic, seasonal decomposition, spatial autocorrelation, and spatio-temporal cluster analyses were performed. Results: A total of 2,220 patients with HFRS were reported over the study period, with an average annual incidence of 1.89/100,000 and a case fatality rate of 2.52%. The male:female ratio was 2.8:1. 75.3% of patients were aged between 16 and 60 years old, 75.3% of patients were farmers, and 11.6% had both "three red" and "three pain" symptoms. The HFRS epidemic showed two-peak seasonality: the primary fall-winter peak and the minor spring peak. The HFRS epidemic presented highly spatially heterogeneous, street/township-level hot spots that were mostly distributed in Huangdao, Pingdu, and Jiaozhou. The spatio-temporal cluster analysis revealed three cluster areas in Qingdao City that were located in the south of Huangdao District during the fall-winter peak. Conclusion: The distribution of HFRS in Qingdao exhibited periodic, seasonal, and regional characteristics, with high spatial clustering heterogeneity. The typical symptoms of "three red" and "three pain" in patients with HFRS were not obvious.

Rare earth elements in the upstream of Yangtze River Delta: Spatio-temporal distributions, sources and speciations.

With the development of high technology, the environmental pollution caused by rare earth elements (REEs) has gradually aroused social concern, especially the water environment. As a upstream tributary in Xin'an river basin, Fengle river has a decisive impact on the downstream Yangtze River Delta. In this study, the spatial and temporal distributions, sources and the speciations of REEs in surface water of wood, agriculture and industry regions of Fengle River in the two seasons were studied. This study provides scientific basis for local government management, give new method in REEs sources identification, and provide data for the global databases.

Spatial and temporal distribution patterns of atmospheric PM2.5 and water-soluble inorganic ions in Shenzhen, China from 2016 to 2021.

In this study, a total of 1425 atmospheric fine particulate matter (PM2.5) samples were collected and analyzed for four water-soluble inorganic ions from 2016-2021 in Shenzhen. The average PM2.5 concentrations exhibit an overall decreasing trend, with a total decrease of 57.5% during the six-year period, which is related to the reduction of emissions due to the 13th Five-Year Plan, the COVID-19, and the introduction of new energy conservation and emission reduction policies. The analyzed ions were all detected in PM2.5 during the observation period in the concentration order of SO42- >NO3- >NH4+ >Cl-. These ions also showed a declining trend over the six years, with the average concentrations of the four ions decreasing to 3.95, 2.25, 1.59, and 0.17 µg m-3 until 2021, respectively. Seasonally, the concentration of SO42- peaks in autumn and winter, and Cl- peaks in spring, while both NO3- and NH4+ peak in winter and spring. Variations in meteorological factors are the main contributors to their different seasonal concentrations. The average mass percentages of SO42-, NO3-, Cl- and NH4+ in PM2.5 are 51.2%, 25.5%, 2.7% and 20.6%, respectively. Three secondary ions (SO42-, NO3- and NH4+) contribute significantly to the formation of PM2.5 as well as having a stronger correlation with it.

Epidemiological characteristics, diagnosis and treatment effect of rifampicin-resistant pulmonary tuberculosis (RR-PTB) in Guizhou Province.

BACKGROUND: Rifampicin-resistant pulmonary tuberculosis (RR-PTB) presents a significant threat to global public health security. China bears a substantial burden of RR-PTB cases globally, with Guizhou Province experiencing particularly alarming trends, marked by a continual increase in patient numbers. Understanding the population characteristics and treatment modalities for RR-PTB is crucial for mitigating morbidity and mortality associated with this disease. METHODS: We gathered epidemiological, diagnostic, and treatment data of all RR-PTB cases recorded in Guizhou Province from January 1, 2017 to December 31, 2023. Utilizing composition ratios as the analytical metric, we employed Chi-square tests to examine the spatiotemporal distribution patterns of RR-PTB patients and the evolving trends among different patient classifications over the study period. RESULTS: In our study, 3396 cases of RR-PTB were analyzed, with an average age of 45 years. The number of RR-PTB patients rose significantly from 176 in 2017 to 960 in 2023, peaking notably among individuals aged 23-28 and 44-54, with a rising proportion in the 51-80 age group (P < 0.001). Since 2021, there has been a notable increase in the proportion of female patients. While individuals of Han ethnic group comprised the largest group, their proportion decreased over time (P < 0.001). Conversely, the Miao ethnicity showed an increasing trend (P < 0.05). The majority of patients were farmers, with their proportion showing an upward trajectory (P < 0.001), while students represented 4.33% of the cases. Geographically, most patients were registered in Guiyang and Zunyi, with a declining trend (P < 0.001), yet household addresses primarily clustered in Bijie, Tongren, and Zunyi. The proportion of floating population patients gradually decreased, alongside an increase in newly treated patients and those without prior anti-tuberculosis therapy. Additionally, there was a notable rise in molecular biological diagnostic drug sensitivity (real-time PCR and melting curve analysis) (P < 0.001). However, the cure rate declined, coupled with an increasing proportion of RR-PTB patients lost to follow-up and untreated (P < 0.05). CONCLUSIONS: Enhanced surveillance is crucial for detecting tuberculosis patients aged 23-28 and 44-54 years. The distribution of cases varies among nationalities and occupations, potentially influenced by cultural and environmental factors. Regional patterns in RR-PTB incidence suggest tailored prevention and control strategies are necessary. Despite molecular tests advances, challenges persist with low cure rates and high loss to follow-up. Strengthening long-term management, resource allocation, and social support systems for RR-PTB patients is essential.

Spatio-temporal distribution of rhinovirus types in Kenya: a retrospective analysis, 2014.

The epidemiology and circulation patterns of various rhinovirus types within populations remains under-explored. We generated 803 VP4/VP2 gene sequences from rhinovirus-positive samples collected from acute respiratory illness (ARI) patients, including both in-patient and outpatient cases, between 1st January and 31st December 2014 from eleven surveillance sites across Kenya and used phylogenetics to characterise virus introductions and spread. RVs were detected throughout the year, with the highest detection rates observed from January to March and June to July. We detected a total of 114 of the 169 currently classified types. Our analysis revealed numerous virus introductions into Kenya characterized by local expansion and extinction, and extensive spatial mixing of types within the country due to the widespread transmission of the virus after an introduction. This work demonstrates that in a single year, the circulation of rhinovirus in Kenya was characterized by substantial genetic diversity, multiple introductions, and extensive geographical spread.

[Water Quality Evaluation and Spatial-temporal Distribution Characteristics of the East Route of the South-to-North Water Diversion Project in Jiangsu Province Based on WQI].

The east route of the South-to-North Water Diversion Project in Jiangsu Province was used as an example to investigate the characteristics of changes in water quality in the area affected by significant water diversion projects. Based on the comprehensive assessment method of the water quality index （WQI）, the M-K trend test, and the geographic information system （GIS）, the spatial and temporal variation characteristics of water quality in the 13 national assessment sections of the east route of the South-to-North Water Diversion Project in Jiangsu Province were evaluated and analyzed from 2013 to 2022. The results showed that the water quality assessment grades in the study area were mainly "medium" and "good." The overall mean value was 74.03, indicating "good" water quality. DO, BOD5, and NH4+-N were the primary indicators of changes in river water quality in the region. Over time, the water quality in the basin had significantly improved from 2013 to 2022. However, there was a rebound in 2021, but this phenomenon was effectively controlled in 2022. Water quality was better during the non-flood season compared to that during the flood season. From a spatial perspective, the water quality in the southern part of the region was superior to that in the north. Furthermore, the rate of improvement was faster in the south. This could be attributed to factors such as precipitation, population density, and the scale of agricultural and livestock activities. However, the water quality of the S11 （Sanduoxi Bridge） section was generally lower than that of other sections and should be given further attention.

Legacy and alternative per- and polyfluoroalkyl substances spatiotemporal distribution in China: Human exposure, environmental media, and risk assessment.

In recent decades, China's rapid development has led to significant environmental pollution from the widespread use of chemical products. Per- and polyfluoroalkyl substances (PFAS) are among the most concerning pollutants due to their persistence and bioaccumulation. This article assesses PFAS exposure levels, distribution, and health risks in Chinese blood, environment, and food. Out of 4037 papers retrieved from November 2022 to December 31, 2023, 351 articles met the criteria. Findings show perfluorooctanoic acid (PFOA) and perfluorooctane sulfonic acid (PFOS) as the main PFAS in both Chinese populations and the environment. The highest PFOA levels in Chinese populations were in Shandong (53.868 ng/mL), while Hubei had the highest PFOS levels (43.874 ng/mL). Similarly, water samples from Sichuan (2115.204 ng/L) and Jiangsu (368.134 ng/L) had the highest PFOA and PFOS levels, respectively. Although localized areas showed high PFAS concentrations. Additionally, developed areas had higher PFAS contamination. The researches conducted in areas such as Qinghai and Hainan remain limited, underscoring the imperative for further investigation. Temporal analysis indicates declining levels of some PFAS, but emerging alternatives require more research. Limited studies on PFAS concentrations in soil, atmosphere, and food emphasize the need for comprehensive research to mitigate human exposure.

Unseen riverine risk: Spatio-temporal shifts of microplastic pollution and its bioavailability in freshwater fish within the Ikopa River urban system.

Growing concern over microplastic pollution, driven by their widespread accumulation in the environment, stresses the need for comprehensive assessments. This study investigates the spatial and temporal distribution of microplastics in the Ikopa River (Antananarivo - Madagascar), which flows through a densely populated area, and examines their correlation with contamination levels in local fish species. By analyzing upstream and downstream stations across wet and dry seasons, only a notable increase in microplastic concentration downstream during the wet season was observed, ranging from 138.6 ± 9.0 to 222.0 ± 24.5 particles m-3, with polyethylene-co-vinyl acetate being the predominant polymer at 62.3 ± 5.13% of the total sampled polymers. This distribution underlines the impact of urban activities on pollution levels. Fish species, gambusia and Nile tilapia, were assessed for microplastic occurrence in gills and gastrointestinal tracts. Higher contamination rates were found in gambusia, enlightening the influence of feeding behaviour and fish habitat on microplastics contamination. Ingestion of microplastics directly from the water column was evident in both species, with the detection of high-density plastics such as polytetrafluoroethylene and polyvinyl chloride suggesting likely sediment contamination. This research highlights the widespread contamination of aquatic environments and its direct impact on local wildlife, pointing to a clear requirement for effective pollution management strategies.

COVID-19 lockdown measures affect polycyclic aromatic hydrocarbons distribution and sources in sediments of Chaohu Lake, China.

The COVID-19 pandemic has profoundly impacted human activities and the environment globally. The lockdown measures have led to significant changes in industrial activities, transportation, and human behavior. This study investigates how the lockdown measures influenced the distribution of polycyclic aromatic hydrocarbons (PAHs) in the sediments of Chaohu Lake, a semi-enclosed lake. Surface sediment samples were collected in summer of 2020 (lockdown have just been lifted) and 2022 and analyzed for 16 priority PAHs. The range of ΣPAHs concentrations remained similar between 2020 (158.19-1693.64 ng·g-1) and 2022 (148.86-1396.54 ng·g-1). Among the sampling sites, the west lake exhibited similar PAHs concentrations characteristics over the two years, with higher levels observed in areas near Hefei City. However, the east lake exhibited increased ΣPAHs concentrations in 2022 compared to 2020, especially the area near ship factory. PAHs source analysis using principal component analysis-multiple linear regression (PCA-MLR) revealed an increased proportion of petroleum combustion sources in 2022 compared to 2020. The isotope analysis results showed that organic matter (OM) sources in the western lake remained relatively stable over the two years, with sewage discharge dominating. In contrast, the eastern lake experienced a shift in OM sources from sewage to C3 plants, potentially contributing to the increased PAH levels observed in the eastern lake sediments. Ecological risk assessment revealed low to moderate risk in both 2020 and 2022. Health risk evaluation indicated little difference in incremental lifetime cancer risk (ILCR) values between the two years, with only benzo[a]pyrene (BaP) posing a high risk among the carcinogenic PAHs. Children generally faced higher health risks compared to adults. This study reveals pandemic-induced changes in PAH pollution and sources in lake sediments, offering new insights into the impact of human activities on persistent organic pollutants, with implications for future pollution control strategies.

Pedestrian flow-environmental pollutants interactions and health risks to residents in high-occupancy public areas of apartment buildings.

The current interaction of pedestrian flow and environmental pollutants in high-occupancy public areas of apartment and the risks of residents being exposed to environmental pollutants are issues that are often overlooked but urgently need to be addressed. In this study, we provide a comprehensive of pedestrian flow-environmental pollutants interactions and health risks to residents in first-floor public areas of apartment with high-occupancy. The main findings indicate that under closed management conditions, there is a significant increase in TVOC and noise levels during the peak periods of nighttime pedestrian flow. In the correlation analysis, the significant impact of time granularity selection in clarifying the correlation between pedestrian flow and environmental pollutants has been highlighted, with larger time granularities generally showing stronger correlations, while finer time granularities may help identify specific risks in areas directly connected to the external environment. There is a significant correlation exists between pedestrian flow and environmental pollutants (TVOC, ozone, and noise), with higher concentrations of these pollutants observed during peak pedestrian flow periods, thereby increasing the risk of residents being exposed to adverse environmental conditions. To mitigate the risks associated with TVOC pollution and noise exposure, it is crucial to maintain proper ventilation, avoid conducting cleaning or maintenance activities during peak hours, and implement noise-reducing measures, such as distancing noise sources from residential areas or installing soundproofing barriers. Additionally, the study identifies total volatile organic compounds originating from property maintenance activities and clarifies their dispersion patterns, emphasizing the importance of developing robust, standardized maintenance protocols for indoor environmental quality assurance. This research can improve the environmental sustainability of apartment buildings and provide a theoretical basis for the development of environmental health strategies for high-occupancy public areas of apartment buildings.

Spatial and Temporal Distribution Characteristics and Cytotoxicity of Atmospheric PM2.5 in the Main Urban Area of Lanzhou City.

PM2.5, as one of the most harmful pollutant in the atmospheric environment and population health, has received much attention. We monitored PM2.5 levels at five sampling sites in the Lanzhou City and collected PM2.5 particles from two representative sites for cytotoxicity experiment. The cytotoxicity of PM2.5 samples on A549 cells and migration ability of the cells were respectively detected by Cell Counting kit-8 (CCK-8) assay and scratch assay. We detected the levels of cellular inflammatory factors and oxidative damage-related biochemical indexes. RT-qPCR was used to detect the mRNA levels of NF-κB and epithelial-mesenchymal transition (EMT)-related genes. We found that the Lanlian Hotel station had the highest PM2.5 annual average concentration. The annual average concentration change curve of PM2.5 showed a roughly "U"-shaped distribution during the whole sampling period. The cytotoxicity experiment showed the viability of A549 cells decreased and the scratch healing rate increased in the 200 and 400 µg/mL PM2.5-treated groups. We also found 400 µg/mL PM2.5 induced changes in the mRNA levels of NF-κB and EMT-related genes, the mRNA levels of IKK-α, NIK, and NF-κB in the 400 µg/mL PM2.5 group were higher than those in the control group. The mRNA levels of E-cadherin decreased and α-SMA increased in the 400 µg/mL PM2.5 groups, and the mRNA levels of Fibronectin increased in the 400 µg/mL PM2.5 groups. Moreover, we found hydroxyl radical scavenging ability and T-AOC levels were lower, and LPO levels were higher in the 200 and 400 µg/mL PM2.5 groups, and the SOD activity of cells in the 400 µg/mL PM2.5 group decreased. And compared with the control group, the levels of TNF-α were higher in the 200 and 400 µg/mL PM2.5 groups and the levels of IL-1 were higher in the 400 µg/mL PM2.5 group. The results indicated that the cytotoxicity of atmospheric PM2.5 was related to oxidative damage, inflammatory response, NF-κB activity and EMT.

Pan India fluoride hazard assessment in groundwater.

Fluoride (F¯) contamination in groundwater in India has gained global attention due to human health hazards. India's hydrogeological heterogeneity, spatio-temporal variability of F¯, and health hazards due to geogenic and geo-environmental control pose unique challenges. Addressing these with only a single region-specific study is not possible. Therefore, this study provides an in-depth, holistic analysis of pan India F¯ contamination, controlling factors, and health hazards using a coupled advanced geostatistical and geospatial approach. Alarming F¯ contaminations are identified in Rajasthan, Telangana, Western Andhra Pradesh, Eastern Karnataka, Parts of Haryana, Gujarat, Madhya Pradesh, Tamil Nadu, Uttar Pradesh, Jharkhand, Bihar, and Chhattisgarh. Probabilistic health-risk evaluation using hot-spot, showed similar spatio-temporal distribution of F¯ contamination. The hazard quotient (HQ) for high F¯ shows more adversity to children than adults. Nationally, 8.65 % and 7.10 % of pre- and post-monsoon sites exceed the recommended safe limit of 1.50 mg/L. The highest average F¯ concentration is in Rajasthan. Very high-risk skeletal fluorosis is possible at around ≤ 2 %, whereas dental caries due to deficiency in F¯ concentration is approximately 40 %. A decisive hierarchy of lithology, geomorphology, soils, and lineaments control are identified on F¯ contamination. Climatic conditions are pivotal in governing all these controlling variables. Thus, in arid/semi-arid dry western regions, F¯ contamination is much higher than in the humid areas. Integration of strengths, weaknesses, opportunities, and threats (SWOT) analysis with the results can aid policymakers and government authorities in achieving sustainable remedial measures for future adaptability.

Spatial-temporal variation and source analysis of heavy metals in different land use types in Beilun District (2015 and 2022).

The soil environment plays an important role in urban ecosystems. To study the heavy metal contamination of soil in Beilun District, Ningbo, we collected soil samples from 60 points in urban and peri-urban areas of Beilun District and analyzed the spatiotemporal variation and sources of heavy metal pollution in various land-use types. The results shown that the heavy metal contents in 2015 and 2022 were higher than the background soil values of Ningbo city, and there was an accumulation of heavy metals over these 7 years. The contents of heavy metals in green belts and woodland in 2022 were higher than those in 2015, while there was no significant change in agricultural land. The heavy metal contents in both years were mainly in the order green belts > agricultural land > woodland. The spatiotemporal distribution of heavy metal content showed that heavy metal pollution in Beilun District was concentrated in five industrial areas, and there was a trend toward the disappearance of highly polluted points. But the single-factor pollution index, pollution load index (PLI), and geoaccumulation index (Igeo) indicated that there was no significant heavy metal pollution in Beilun District, and individual elements at specific points showed slight pollution. The source analysis results showed that the main source of Hg is chemical, As is mainly derived from agricultural, Cr, Ni and Cu are mainly derived from natural, the main sources of Zn and Cd are electroplating and machinery activities, and the main source of Pb is traffic. These results specify a reference for future investigation on urban soil heavy metals, and the source apportionment results provide a scientific foundation for subsequent soil heavy metal pollution treatment.

Dynamics of Noctiluca scintillans blooms: A 20-year study in Jangmok Bay, Korea.

This 20-year study (2001-2020) conducted in Jangmok Bay, Korea, assessed the intricate relationships between environmental factors and Noctiluca scintillans blooms. Granger causality tests and PCA analysis were used to assess the impact of sea surface temperature (SST), salinity, dissolved oxygen (DO) concentration, wind patterns, rainfall, and chlorophyll-a (Chl-a) concentration on bloom dynamics. The results revealed significant, albeit delayed, influences of these variables on bloom occurrence, with SST exhibiting a notable 2-month lag and salinity a 1-month lag in their impact. Additionally, the analysis highlighted the significant roles of phosphate, ammonium, and silicate, which influenced N. scintillans blooms with lags of 1 to 3 months. The PCA demonstrates how SST and wind speed during spring and summer, along with wind direction and salinity in winter, significantly impact N. scintillans blooms. We noted not only an increase in large-scale N. scintillans blooms but also a cyclical pattern of occurrence every 3 years. These findings underscore the synergistic effects of environmental factors, highlighting the complex interplay between SST, salinity, DO concentration, and weather conditions to influence bloom patterns. This research enhances our understanding of harmful algal blooms (HABs), emphasizing the importance of a comprehensive approach that considers multiple interconnected environmental variables for predicting and managing N. scintillans blooms.

Understanding stratification and turnover dynamics of a tropical lake using extensive field observations and 3D hydrodynamic simulations.

The stratification and turnover dynamics of a tropical lake were evaluated using field observations and 3D hydrodynamic simulations. Located in the Philippines, Sampaloc Lake is a 104-ha and 27-m deep volcanic crater lake with enclosed watershed, which is at risk of the impacts of intensive aquaculture, rapid urbanization and climate change. Temperature, dissolved oxygen (DO) and chlorophyll-a (Chl-a) were measured at seven sampling stations using a multiprobe. Kruskal-Wallis test revealed that the three parameters are not significantly different among stations, indicating that one sampling station can represent the water quality of the whole lake. Schmidt's Stability Index (SSI) and thermocline strength, together with DO and Chl-a gradients decreased from October 2022 (stratified) to January 2023 (turnover). After successfully verifying the 3D numerical model, sensitivity analyses of water temperature to varying weather, together with particle tracking simulations, were implemented to determine the timing of isothermal state, upwelling, partial mixing, and full turnover. Compared to air temperature, variations in wind speed have more pronounced effects on the delay or progression of isothermal conditions in the lake based on SSI, Lake Number and Wedderburn Number. Isothermal conditions do not necessarily coincide with the timing of full turnover, with the latter being delayed by two days than the former, on average. Results revealed that full turnover can occur several weeks earlier with the decrease in AT and increase in WS. This study can advance the understanding of thermal and turnover dynamics of stratified tropical lakes, leading to better management of the water quality of these water bodies.

[Estimation of Near-surface Ozone Concentration in the Beijing-Tianjin-Hebei Region Based on XGBoost-LME Model].

High spatiotemporal resolution data on near-surface ozone concentration distribution is of great significance for monitoring and controlling atmospheric ozone pollution and improving the living environment. Using TROPOMI-L3 NO2, HCHO products, and ERA5-land high-resolution data as estimation variables, an XGBoost-LME model was constructed to estimate the near-surface ozone concentration in the Beijing-Tianjin-Hebei Region. The results showed that： ① Through correlation analysis, surface 2 m temperature （T2M）, 2 m dewpoint temperature （D2M）, surface solar radiation downwards （SSRD）, tropospheric formaldehyde （HCHO）, and tropospheric nitrogen dioxide （NO2） were important factors affecting the near-surface ozone concentration in the Beijing-Tianjin-Hebei Region. Among them, T2M, SSRD, and D2M had strong correlations, with correlation coefficients of 0.82, 0.75, and 0.71, respectively. ② Compared with that of other models, the XGBoost-LME model had the best performance in terms of various indicators. The ten-fold cross-validation evaluation indicators R2, MAE, and RMSE were 0.951, 9.27 µg·m-3, and 13.49 µg·m-3, respectively. At the same time, the model performed well at different time scales. ③ In terms of time, there was a significant seasonal difference in near-surface ozone concentration in the Beijing-Tianjin-Hebei Region in 2019, with the concentration changing in the order of summer > spring > autumn > winter. The monthly average ozone concentration in the region showed an inverted "V" trend, with a slight increase in September. The highest value occurred in July, whereas the lowest value occurred in December. In terms of spatial distribution, the near-surface ozone concentrations in the Beijing-Tianjin-Hebei Region during the months of February and March were generally at the same levels. In January, November, and December, there was a relatively insignificant trend of higher concentrations in the north and lower concentrations in the south. For the remaining months, the spatial distribution of near-surface ozone concentrations in this area predominantly exhibited a pattern of higher concentrations in the south and lower concentrations in the north. High-value areas were predominantly found in the plain regions of the southern part with lower altitudes, dense population, and higher industrial emissions； low-value areas, on the other hand, were primarily located in mountainous areas of the northern part with higher altitudes, sparse population, higher vegetation coverage, and lower industrial emissions.

[Spatial and Temporal Distribution of Antibiotic Resistance Genes in Different River Sediments in Typical Cities: A Case Study of Shijiazhuang].

Rivers are important reservoirs of antibiotic resistance genes (ARGs). However, most current studies have focused on the temporal and spatial distribution, and data on the differences in the species and abundance of ARGs between urban and rural rivers is still lacking for certain areas. In view of this, two rural rivers and three urban rivers were selected in Shijiazhuang City. In both December 2020 and April 2021, sediments were collected at 15 sampling sites. Metagenomic sequencing technology was used to compare the differences in temporal-spatial variation for ARGs in sediments. The results showed that:① 162 and 79 ARGs were detected in urban (4 776 ±4 452) and rural rivers (1 043 ±632), respectively. The abundance and species of ARGs in urban rivers were higher than those in rural rivers. ② The relative abundances of sulfonamide (SAs,27 %), aminoglycoside (AGs,26 %), and multidrug (MDs,15 %) ARGs had the highest abundance in urban rivers, whereas the relative abundance of MDs ARGs was highest in rural rivers (65 %). On the whole, the complexity of ARGs in urban rivers was higher than that in rural rivers. ③ There was a significant positive correlation between SAs, AGs, MDs, tetracycline, phenicol, macrolides-lincosamids-streptogramins (MLS), ß-lactams, and diaminopyrimidine ARGs in urban rivers (P < 0.01); however, there was a significant negative correlation between glycopeptide ARGs and all types of ARGs (P < 0.05 and P < 0.01). There was a significant positive correlation between MDs and SAs ARGs in rural rivers (P < 0.05), but there was a significant negative correlation between amino aminocoumarin, peptide, rifamycin, and fosfomycin ARGs (P < 0.05 and P < 0.01). ④ For the temporal variation in urban rivers, 162 ARGs (4 776 ±4 452) and 148 ARGs (5 673 ±5 626) were detected in December and April, respectively. For the temporal variation in rural rivers, 79 species (1 043 ±632) and 46 species (467 ±183) were detected in December and April, respectively. ⑤ RDA analysis results showed that the spatial-temporal distributions of ARGs in urban and rural rivers were different. Correlation analysis showed that the ARGs in urban rivers were significantly correlated with the number of industrial enterprises, whereas the ARGs in rural rivers were significantly correlated with the output value of animal husbandry. In general, this study identified the main influencing factors for ARGs in different rivers and provided data support for ARGs risk management in different rivers.

Global distribution of zoonotic digenetic trematodes: a scoping review.

BACKGROUND: Digenetic trematodes, including blood flukes, intestinal flukes, liver flukes, lung flukes, and pancreatic flukes, are highly diverse and distributed widely. They affect at least 200 million people worldwide, so better understanding of their global distribution and prevalence are crucial for controlling and preventing human trematodiosis. Hence, this scoping review aims to conduct a comprehensive investigation on the spatio-temporal distribution and epidemiology of some important zoonotic digenetic trematodes. METHODS: We conducted a scoping review by searching PubMed, Web of Science, Google Scholar, China National Knowledge Infrastructure, and Wanfang databases for articles, reviews, and case reports of zoonotic digenetic trematodes, without any restrictions on the year of publication. We followed the inclusion and exclusion criteria to identify relevant studies. And relevant information of the identified studies were collected and summarized. RESULTS: We identified a total of 470 articles that met the inclusion criteria and were included in the review finally. Our analysis revealed the prevalence and global distribution of species in Schistosoma, Echinostoma, Isthmiophora, Echinochasmus, Paragonimus, Opisthorchiidae, Fasciolidae, Heterophyidae, and Eurytrema. Although some flukes are distributed worldwide, developing countries in Asia and Africa are still the most prevalent areas. Furthermore, there were some overlaps between the distribution of zoonotic digenetic trematodes from the same genus, and the prevalence of some zoonotic digenetic trematodes was not entirely consistent with their global distribution. The temporal disparities in zoonotic digenetic trematodes may attribute to the environmental changes. The gaps in our knowledge of the epidemiology and control of zoonotic digenetic trematodes indicate the need for large cohort studies in most countries. CONCLUSIONS: This review provides important insights into the prevalence and global distribution of some zoonotic digenetic trematodes, firstly reveals spatio-temporal disparities in these digenetic trematodes. Countries with higher prevalence rate could be potential sources of transmitting diseases to other areas and are threat for possible outbreaks in the future. Therefore, continued global efforts to control and prevent human trematodiosis, and more international collaborations are necessary in the future.

[Epidemiological characteristics of leptospirosis in China from 2010 to 2022].

OBJECTIVE: To analyze the epidemiological characteristics of leptospirosis in China from 2010 to 2022, so as to provide insights into formulation of the leptospirosis control strategy. METHODS: All data pertaining to clinically diagnosed cases and confirmed cases of leptospirosis reported in China from January 1, 2010 to December 31, 2022 was collected from Chinese Disease Prevention and Control Information Management System. The spatial, temporal and population distributions, and report and diagnosis institutions of leptospirosis cases were analyzed using a descriptive epidemiological method. RESULTS: A total of 4 559 leptospirosis cases were reported in China from 2010 to 2022, with an annual average number of 351 cases, and the number of reported leptospirosis cases reduced from 679 cases in 2010 to 158 cases in 2018. A total of 4 276 leptospirosis cases were reported in Sichuan Province, Yunnan Province, Guangdong Province, Hunan Province, Fujian Province, Zhejiang Province, Guangxi Zhuang Autonomous Region, Anhui Province, Jiangxi Province and Guizhou Province, accounting for 93.79% of the total number of leptospirosis cases in China. The number of leptospirosis cases had recently appeared a remarkable decline in Yunnan Province, while a significant rise was seen in the number of leptospirosis cases in two provinces of Zhejiang and Guangdong. No leptospirosis cases were reported in Henan Province from 2010 to 2020; however, there were 5 cases and 2 cases reported in 2021 and 2022, respectively. There was only one leptospirosis case reported in Shaanxi Province from 2010 to 2017; however, leptospirosis cases were reported in the province for 5 consecutive years since 2018. Leptospirosis cases were reported throughout the year in China from 2010 to 2022, with the peak of incidence found during the period between August and October, and the peak of leptospirosis incidence varied in provinces. A higher number of leptospirosis cases was seen among men than among women, with a male to female ratio of 2.3:1, and the median age of leptospirosis cases was 50 years (interquartile range, 23 years), with the highest proportion of leptospirosis cases reported at ages of 51 to 60 years (23.21%). Among all reported leptospirosis cases, 53.28% were confirmed cases, and the proportion of confirmed cases increased from 35.05% in 2010 to 61.66% in 2022. In addition, there were 67.22% of leptospirosis cases (2 937 cases) reported by comprehensive hospitals, 20.44% (893 cases) by disease control and prevention institutions, 7.23% (316 cases) by grassroots healthcare institutions and 5.10% (223 cases) by other healthcare and medical institutions, and the mortality of reported leptospirosis cases was 1.07% in China from 2010 to 2022, with a higher mortality seen among men than among women (1.39% vs. 0.36%; χ2 = 9.52, P = 0.002). CONCLUSIONS: The incidence of leptospirosis remained at a low level in China from 2010 to 2022, and southern China was still the main endemic area for leptospirosis. The epidemiological characteristics of leptospirosis cases varied in endemic provinces, and leptospirosis cases had been continued to be reported in Shaanxi and Henan provinces, which should be paid much attention to. Intensified surveillance of leptospirosis, improved diagnosis and treatment capability of leptospirosis cases and leptospirosis control with adaptations to local circumstance are recommended.

Wastewater threshold as an indicator of COVID-19 cases in correctional facilities for public health response: A modeling study.

Although prison facilities are not fully isolated from the communities in which they are located, most of the population is confined and requires high levels of health vigilance and protection. This study aimed to examine the dynamic relationship between facility-level wastewater viral concentrations and the probability of at least one positive COVID-19 case within the facility. The study period was from January 11, 2021 to May 8, 2023. Wastewater samples were collected and analyzed for SARS-CoV-2 (N1) and pepper mild mottle virus (PMMoV) three times weekly across 14 prison facilities in Kentucky (USA). Positive clinical case reports were also provided. A hierarchical Bayesian facility-level temporal model with a latent lagged process was developed. We modeled facility-specific SARS-CoV-2 (N1) normalized by the PMMoV wastewater concentration ratio threshold associated with at least one COVID-19 clinical case at an 80 % probability. The threshold differed among facilities. Across the 14 facilities, our model demonstrates a mean capture rate of 94.95 % via the N1/PMMoV ratio threshold with pts≥0.5. However, as the pts threshold was set higher, such as at ≥0.9, the mean capture rate of the model was reduced to 60 %. This robust performance underscores the effectiveness of the model for accurately detecting the presence of positive COVID-19 cases among incarcerated people. The findings of this study provide a facility-specific threshold model for public health response based on frequent wastewater surveillance.