ABSTRACT
BACKGROUND: The link between the prostate microbiome and prostate cancer remains unclear. Few studies have analyzed the microbiota of prostate tissue, and these have been limited by potential contamination by transrectal biopsy. Transperineal prostate biopsy offers an alternative and avoids fecal cross-contamination. We aim to characterize the prostate microbiome using transperineal biopsy. METHODS: Patients with clinical suspicion for prostate cancer who were to undergo transperineal prostate biopsy with magnetic resonance imaging (MRI) fusion guidance were prospectively enrolled from 2022 to 2023. Patients were excluded if they had Prostate Imaging Reporting and Data System lesions with scores ≤ 3, a history of prostate biopsy within 1 year, a history of prostate cancer, or antibiotic use within 30 days of biopsy. Tissue was collected from the MRI target lesions and nonneoplastic transitional zone. Bacteria were identified using 16S ribosomal RNA gene sequencing. RESULTS: Across the 42 patients, 76% were found to have prostate cancer. Beta diversity indices differed significantly between the perineum, voided urine, and prostate tissue. There were no beta diversity differences between cancerous or benign tissue, or between pre- and postbiopsy urines. There appear to be unique genera more abundant in cancerous versus benign tissue. There were no differences in alpha diversity indices relative to clinical findings including cancer status, grade, and risk group. CONCLUSIONS: We demonstrate a rigorous method to better characterize the prostate microbiome using transperineal biopsy and to limit contamination. These findings provide a framework for future large-scale studies of the microbiome of prostate cancer.
Subject(s)
Microbiota , Perineum , Prostate , Prostatic Neoplasms , Humans , Male , Prostatic Neoplasms/pathology , Prostatic Neoplasms/microbiology , Prostate/pathology , Prostate/microbiology , Prostate/diagnostic imaging , Prospective Studies , Middle Aged , Aged , Perineum/microbiology , Perineum/pathology , Magnetic Resonance Imaging/methods , Biopsy/methods , Image-Guided Biopsy/methods , RNA, Ribosomal, 16S/analysis , RNA, Ribosomal, 16S/geneticsABSTRACT
BACKGROUND: Maternal Group B Streptococcus (GBS) colonization is influenced by many factors but results are inconsistent. Consideration of antenatal risk factors may help inform decision making on GBS microbiological culture screening where universal screening is not standard of care. We sought to identify independent predictors of GBS colonization at 34-37 weeks gestation incorporating vaginal symptoms, perineal hygiene measures, sexual activity, and a potential novel factor, constipation. METHODS: In this prospective cross-sectional study, 573 women at 34-37 weeks gestation had an ano-vaginal swab taken and sent for selective culture for GBS. Women were asked about vaginal bleeding, discharge, irritation and candidiasis, antibiotic use during pregnancy, ano-vaginal hygiene practices such as douching and perineal cleansing after toileting, sexual intercourse related activities, and a potential novel factor for GBS carriage, constipation. Maternal basic demographics and obstetric-related characteristics were also collected. Bivariate analyses were performed to identify associates of GBS colonization. All variables with p < 0.05 found on bivariate analysis were then included into a model for multivariable binary logistic regression analysis to identify independent risk factors for GBS colonization. RESULTS: GBS colonization was found in 235/573 (41.0%) of participants. Twenty six independent variables were considered for bivariate analysis. Eight were found to have p < 0.05. Following adjusted analysis, six independent predictors of GBS colonization were identified: ethnicity, previous neonatal GBS prophylaxis, antenatal vaginal irritation, antibiotic use, recent panty liner use, and frequency of sexual intercourse. Vaginal discharge and perineal cleansing were not associated after adjustment. Recent douching and constipation were not associated on bivariate analysis. CONCLUSION: The identification of independent predictors of GBS colonization in late pregnancy may inform the woman and care provider in their shared decision making for microbiological screening at 35-38 weeks gestation in locations where universal GBS screening is not standard of care. ETHICS OVERSIGHT: This study was approved by the Medical Ethics Committee of University Malaya Medical Centre (UMMC) on August 9, 2022, reference number 2022328-11120.
Subject(s)
Constipation , Hygiene , Perineum , Pregnancy Complications, Infectious , Sexual Behavior , Streptococcal Infections , Streptococcus agalactiae , Vagina , Humans , Female , Pregnancy , Prospective Studies , Streptococcus agalactiae/isolation & purification , Adult , Constipation/microbiology , Constipation/prevention & control , Vagina/microbiology , Cross-Sectional Studies , Pregnancy Complications, Infectious/microbiology , Pregnancy Complications, Infectious/prevention & control , Pregnancy Complications, Infectious/diagnosis , Streptococcal Infections/prevention & control , Streptococcal Infections/diagnosis , Perineum/microbiology , Perineum/injuries , Risk Factors , Anal Canal/microbiology , Pregnancy Trimester, ThirdABSTRACT
BACKGROUND: Screening for maternal anogenital Group B streptococci (GBS) colonization in pregnancy with initiation of intravenous intrapartum antibiotic prophylaxis as indicated has led to a significant reduction in the incidence of neonatal GBS infection. This study aims to evaluate the agreement between vaginal-perianal or vaginal-perineal culture and the more typically used vaginal-rectal culture for screening for maternal anogenital GBS colonization in the third trimester of pregnancy. METHODS: Eligible English-language studies published until January 2020 were retrieved from Scopus, Web of Science, PubMed, Embase, and ClinicalTrials.gov databases. Studies were compiled that assessed for GBS colonization utilizing vaginal-perianal or vaginal-perineal culture and vaginal-rectal culture during the third trimester of pregnancy. Nonoriginal research articles and studies that did not assess pregnant patients, did not use culture-based screening, or did not compare vaginal-perianal or vaginal-perineal culture with vaginal-rectal culture were excluded. The search identified 559 articles with three prospective cohort studies that met inclusion criteria, including 643 participants. Quality was assessed using the Newcastle-Ottawa Scale, and risk of bias was assessed using the Risk of Bias In Non-randomized Studies of Interventions (ROBINS-I) tool. Patient characteristics and associated pain with specimen collection were abstracted. Meta-analyses of both the raw agreement and the Cohen's kappa statistic were performed. RESULTS: Within the three included studies, the range of GBS detection was 17.6-34.0%, consistent with the anticipated prevalence of GBS colonization reported in earlier publications. For both raw agreement and Cohen's kappa coefficient, the test for heterogeneity was not significant, indicating low heterogeneity among studies. The pooled estimate of the raw agreement was 0.97 (95%CI 0.95-0.98) and of the Cohen's kappa coefficient was 0.91 (95% CI: 0.87-0.95), indicating (according to the Landis and Koch criteria) an "almost perfect" agreement between the compared clinical tests. In the two studies that assessed procedure-related patient discomfort, vaginal-rectal swabbing caused more discomfort. CONCLUSION: Use of vaginal-perineal culture for assessment of maternal GBS colonization is comparable to the more typically utilized vaginal-rectal culture and is associated with less discomfort.
Subject(s)
Mass Screening/methods , Pregnancy Complications, Infectious/diagnosis , Pregnancy Trimester, Third , Specimen Handling/methods , Streptococcal Infections/diagnosis , Streptococcus agalactiae/isolation & purification , Female , Humans , Perineum/microbiology , Pregnancy , Rectum/microbiology , Vagina/microbiologyABSTRACT
Enrichment culture (EC) remains gold standard for detecting MRSA colonisation, but molecular methods shorten turnaround time. The CE-marked automated Hologic Panther Fusion MRSA Assay (HPFM) is validated for nasal swabs. We compared HPFM with EC following an in-house PCR for detection of MRSA in nasal, pharyngeal, and perineal ESwabs. The same ESwabs were analysed using HPFM and inoculated in selective Tryptic Soy Broth (TSB) for overnight incubation. TSBs were screened by a PCR targeting nuc, femA, mecA, and mecC. Only samples with PCR results compatible with MRSA presence were inoculated onto 5% blood agar and chromogenic MRSA plates. HPFM detected MRSA in 103 of 132 EC positive samples indicating a sensitivity of 78.0% across sample types. When paired TSBs of 29 EC positive/HPFM negative samples were re-analysed by HPFM, MRSA was detected in 17/29 TSBs indicating that enrichment will increase the sensitivity of HPFM. HPFM analyses of cultured isolates from the remaining 12 EC positive/HPFM negative samples failed to detect orfX. HPFM reported the presence of MRSA in 22 samples where EC failed to identify MRSA. Fifteen of these ESwabs had been kept and direct culture without enrichment identified MRSA in seven samples. HPFM was useful for all sample sites. Compared to EC, the sensitivity of HPFM was limited because of lack of analytical sensitivity and failure to detect all MRSA variants. Failure of some MRSA-containing samples to enrich in cefoxitin-containing TSB indicates an unappreciated limitation of EC, which may lead to underestimation of the specificity of molecular assays.
Subject(s)
Methicillin-Resistant Staphylococcus aureus/isolation & purification , Nose/microbiology , Perineum/microbiology , Pharynx/microbiology , Staphylococcal Infections/microbiology , Humans , Methicillin-Resistant Staphylococcus aureus/classification , Methicillin-Resistant Staphylococcus aureus/genetics , Methicillin-Resistant Staphylococcus aureus/growth & development , Multiplex Polymerase Chain Reaction , Staphylococcal Infections/diagnosisABSTRACT
Abdominoperineal excision of rectum (APER) is one of the widely used surgical procedures to treat low rectal cancer, benign conditions like Crohn's proctitis with anal involvement and as a salvage procedure for anal cancer. Perineal wound infection is a well-recognised complication following such major surgery. Occurrence of appendicitis in a few weeks' time following such a major surgery is uncommon. However, here we present a rare case report of perforated appendicitis presenting as persistent perineal discharge in an elderly man, following laparoscopic APER for a low rectal tumour. To our knowledge, this is the first time such a rare clinical presentation of appendicitis is reported in the history of medical literature. Through this case report, we aim to highlight the importance of considering such an uncommon presentation in patients with perineal discharge, following APER.
Subject(s)
Abscess/diagnosis , Appendicitis/complications , Escherichia coli Infections/diagnosis , Intestinal Perforation/diagnosis , Pelvic Infection/diagnosis , Postoperative Complications/diagnosis , Proctectomy/adverse effects , Abscess/etiology , Abscess/surgery , Adenocarcinoma/diagnosis , Adenocarcinoma/pathology , Adenocarcinoma/surgery , Aged , Appendectomy , Appendicitis/diagnosis , Appendicitis/surgery , Diagnosis, Differential , Escherichia coli/isolation & purification , Escherichia coli Infections/etiology , Escherichia coli Infections/surgery , Humans , Intestinal Perforation/etiology , Intestinal Perforation/surgery , Male , Pelvic Infection/etiology , Pelvic Infection/surgery , Perineum/microbiology , Perineum/pathology , Perineum/surgery , Postoperative Complications/etiology , Postoperative Complications/surgery , Rectal Neoplasms/diagnosis , Rectal Neoplasms/pathology , Rectal Neoplasms/surgery , Rectum/pathology , Rectum/surgerySubject(s)
Cerebrospinal Fluid/microbiology , Klebsiella Infections/diagnosis , Klebsiella Infections/drug therapy , Klebsiella pneumoniae/drug effects , Testis/microbiology , Anti-Bacterial Agents/therapeutic use , Cefoperazone/therapeutic use , Cerebrospinal Fluid/drug effects , Drug Combinations , Epididymis/diagnostic imaging , Epididymis/microbiology , Epididymis/pathology , Epididymis/surgery , Humans , Klebsiella Infections/blood , Klebsiella Infections/cerebrospinal fluid , Magnetic Resonance Imaging , Male , Meropenem/therapeutic use , Middle Aged , Perineum/diagnostic imaging , Perineum/microbiology , Perineum/pathology , Skull/diagnostic imaging , Skull/drug effects , Skull/pathology , Sulbactam/therapeutic use , Testis/drug effects , Testis/pathology , Testis/surgery , Ultrasonography , Vancomycin/therapeutic useABSTRACT
PURPOSE: Incontinence-associated dermatitis (IAD) due to the prolonged exposure of the skin to urinary, fecal, or double incontinence represents a major clinical practice challenge. The aim of this review was to identify and critically appraise the results of published studies on the etiology and pathophysiology of IAD and highlight the current gaps in empirical evidence. METHODS: Scoping literature review. SEARCH STRATEGY: The electronic databases PubMed, Cumulative Index to Nursing and Allied Health Literature, MEDLINE, and Embase were searched for relevant articles published from 1996 to April 2018. Thirteen studies and review articles related to the etiology and pathophysiology of IAD were identified in our initial review, and 3 studies published subsequent to our initial review were evaluated and included in our final review. FINDINGS: These studies suggest that several etiologic factors contribute to the development of IAD including exposure to urine, stool, or a combination of these substances (dual incontinence), the duration and frequency of exposure, frequent cleaning, and inflammatory responses. Results from the current scoping review showed that despite the increasing interest in IAD, evidence related to the underlying mechanisms causing IAD remains sparse. This paucity represents a clear gap in knowledge and indicates a need for additional research. IMPLICATIONS: Future studies should aim at elucidating: (1) the role of urine and its inherent pH on skin integrity, (2) the role of stool, specific digestive enzymes, and fecal bacteria on skin integrity, (3) the permeability and susceptibility of the skin to damage following frequent cleansing activities and occlusion, and (4) the specific inflammatory response triggered following exposure to urine and fecal matter.
Subject(s)
Dermatitis/etiology , Dermatitis/physiopathology , Fecal Incontinence/complications , Health Knowledge, Attitudes, Practice , Urinary Incontinence/complications , Humans , Perineum/microbiology , Skin CareABSTRACT
Fistula-in-ano is a very common surgical condition, caused by anal cryptoglandular inflammation. Most cases are idiopathic. Other causes such as Crohn's disease, trauma and malignancy are well known. Management of fistula-in-ano is largely surgical, especially if the patient is symptomatic. The goal of surgical therapy is sepsis drainage, delineate anatomy and eradicate the fistula while preserving faecal continence. Establishing the aetiology is also crucial as often a combination of specialist medical therapy is required, for example, in Crohn's disease. We report an extremely unusual case of fistula-in-ano on an elderly man with chronic lymphocytic leukaemia (CLL). Histology from the fistula track demonstrated CLL infiltration. This case, not previously reported on PubMed search, illustrates a good example of joint specialist medical (a haematologist) and surgical effort in successfully treating this symptomatic fistula-in-ano.
Subject(s)
Drainage/methods , Leukemia, Lymphocytic, Chronic, B-Cell/complications , Rectal Fistula/etiology , Rectal Fistula/surgery , Abscess/etiology , Aged , Humans , Magnetic Resonance Imaging/methods , Male , Perineum/diagnostic imaging , Perineum/microbiology , Rectal Fistula/pathology , Watchful Waiting/methodsABSTRACT
PURPOSE: We evaluate the prevalent microorganisms, antibiotic sensitivity patterns and associated outcomes in patients with Fournier's gangrene. MATERIALS AND METHODS: A retrospective chart review of patients with Fournier's gangrene was conducted between October 2011 and April 2018 at our institution. Univariate analysis was performed using the independent t-test or Kruskal-Wallis H test for continuous variables and exact test for categorical variables. RESULTS: Of the 143 patients included in this study, wound culture was available in 131 (92%) patients with a median number of 3 microorganisms per wound. The most commonly grown pathogens were Staphylococcus species (66, 46%), Streptococcus species (53, 37%), Bacteroides species (34, 24%), Candida species (31, 22%), Escherichia coli (28, 20%) and Prevotella species (26, 18%). Most bacteria were sensitive to ampicillin-sulbactam, ceftriaxone, piperacillin-tazobactam, amikacin and cefepime, and resistant to ampicillin, trimethoprim-sulfamethoxazole, levofloxacin and clindamycin. Enterococcus faecalis and Streptococcus anginosus were resistant to vancomycin. The overall Fournier's gangrene mortality count was 14 (10%) patients. No association was noted between the type of infection and Fournier's gangrene severity index, length of hospital stay or mortality. CONCLUSIONS: At our institution Candida is a prevalent pathogen in the wound culture of patients with Fournier's gangrene. The resistance patterns for clindamycin and vancomycin are concerning. Addition of an antifungal agent to the empiric treatment should be considered based on clinical presentation.
Subject(s)
Anti-Bacterial Agents/pharmacology , Bacteria/isolation & purification , Candida/isolation & purification , Fournier Gangrene/microbiology , Anti-Bacterial Agents/therapeutic use , Bacteria/drug effects , Candida/drug effects , Clindamycin/pharmacology , Clindamycin/therapeutic use , Debridement , Drug Resistance, Bacterial , Drug Resistance, Fungal , Female , Fournier Gangrene/diagnosis , Fournier Gangrene/mortality , Fournier Gangrene/therapy , Humans , Length of Stay/statistics & numerical data , Male , Microbial Sensitivity Tests/statistics & numerical data , Middle Aged , Perineum/microbiology , Perineum/surgery , Retrospective Studies , Severity of Illness Index , Tertiary Care Centers/statistics & numerical data , Vancomycin/pharmacology , Vancomycin/therapeutic useABSTRACT
Bacteriophages (phages) play a key role in shaping microbial communities, including those of the human body. Phages are abundant members of the urogenital tract, most often persisting through the lysogenic life cycle as prophages integrated within the genomes of their bacterial hosts. While numerous studies of the urogenital microbiota have focused on the most abundant bacterial member of this niche-Lactobacillus species-very little is known about Lactobacillus phages. Focusing on Lactobacillus jensenii strains from the urinary tract, we identified numerous prophages related to the previously characterized Lv-1 phage from a vaginal L. jensenii strain. Furthermore, we identified a new L. jensenii phage, Lu-1. Evidence suggests that both phages are abundant within the urogenital tract. CRISPR spacer sequences matching to Lv-1 and Lu-1 prophages were identified. While first detected in urinary isolates, the Lu-1 phage was also discovered in L. jensenii isolates from vaginal and perineal swabs, and both phages were found in metagenomic data sets. The prevalence of these phages in the isolates suggests that both phages are active members of the urogenital microbiota.
Subject(s)
Bacteriophages/isolation & purification , Lactobacillus/virology , Perineum/microbiology , Vagina/microbiology , Bacteriophages/genetics , Computational Biology , Female , Humans , MicrobiotaABSTRACT
Abstract Background Mycobacterial infections are a serious public health problem worldwide. Involvement of the anal canal and perineum is very rare, but constitute an important differential diagnosis with other equally serious pathologies that may affect the region, such as malignant neoplasms and Crohn's disease. Objectives To conduct a literature review on mycobacterial infections of the perianal region considering the most recent information for diagnostic and therapeutic guidance of this disease. Methods Research was performed on the PUBMED and LILACS databases with the expressions Mycobacterium, Anal, Infection and Tuberculosis. We reviewed articles referring to series of treated cases, clinical reports and literature review published since 2005. Results Information was compiled on the epidemiology of mycobacterial infections; the clinical behavior of affected individuals; diagnostic options and their validity in clinical practice; and, finally, therapeutic options. Conclusions Mycobacterial infections of the anus and perineum are rare. The most common clinical presentations are the presence of ulceration and fistulization. The diagnosis involves more than one procedure for identifying the bacilli and should consider the presence of manifestations in more than one organ. The treatment is based on pharmacological intervention. Surgery is recommended for acute complications or chronic sequelae of the disease.
Resumo Introdução Infecções micobacterianas constituem um grave problema de saúde pública a nível mundial. As manifestações anoperineais são raras, mas constituem um importante diagnóstico diferencial com outras patologias igualmente graves que podem acometer a região, como as neoplasias malignas e a doença de Crohn. Objetivos Realizar um levantamento da literatura sobre infecções micobacterianas da região anoperineal, considerando as informações mais atuais para orientação diagnóstica e terapêutica dessa enfermidade. Métodos Foi realizada pesquisa nos bancos de dados PUBMED e LILACS com as expressões Mycobacterium, Anal, Infection e Tuberculosis. Foram revisados artigos referentes a séries de casos tratados, relatos clínicos e revisão da literatura publicada a partir de 2005. Resultados Foram compiladas informações sobre a epidemiologia das infecções micobacterianas; o comportamento clínico dos indivíduos afetados; opções diagnósticas e sua validade na prática clínica; e, por fim, opções terapêuticas. Conclusões Infecções micobacterianas da região anoperineal são raras. As apresentações clínicas mais comuns são a formação de ulceras e a fistulização. O diagnóstico envolve mais de um procedimento para identificação dos bacilos, e deve considerar a presença de manifestações em mais de um órgão. O tratamento é principalmente medicamentoso, sendo a cirurgia recomendada nas complicações agudas ou sequelas crônicas da doença.
Subject(s)
Humans , Anus Diseases/diagnosis , Mycobacterium Infections/diagnosis , Anal Canal/microbiology , Anus Diseases/therapy , Anus Diseases/epidemiology , Perineum/microbiology , Skin Ulcer/microbiology , Tuberculosis/diagnosis , Tuberculosis/therapy , Tuberculosis/epidemiology , Fissure in Ano/microbiology , Mycobacterium Infections/therapy , Mycobacterium Infections/epidemiologyABSTRACT
Introduction: Streptococcus agalactiae is the main etiological agent causing invasive infection of the newborn with symptoms that may be associated with septicemia, pneumonia or meningitis and prevalences up to 50% worldwide where there is an increase in antibiotic resistance. Objective: To estimate the prevalence of vagino-rectal colonization by S. agalactiae and its sensitivity profile in pregnant women attending a third-level hospital. Materials and methods: One hundred and twenty one pregnant women were sampled by vaginal and rectal swabs. The cultures were carried out following the methodology recommended by the CDC, and chromID Strepto B agar was added. The suggestive colonies were identified biochemically and the sensitivity profiles according to CLSI were determined. As control, S. pneumoniae ATCC 49619 and S. agalactiae ATCC 12403 were used. Results: Pregnant colonization prevalence by S. agalactiae was 20.66%, with a total of 40 isolates of which 12.5% were non-sensitivity to penicillin. Sensitivity to levofloxacin, clindamycin and erythromycin was 100%, 92.5% y 87.5%, respectively, with the phenotypes iMLSB (3/40) and M (2/40). No sensitivity to tetracycline was found. Conclusions: The prevalence of vagino-rectal colonization by S. agalactiae in the study population was 20.66%, obtaining isolates not sensitive to penicillin and resistance to macrolides and lincosamidas by the Kirby-Bauer technique, so the importance of carrying out active active in pregnant women colonized by Streptococcus agalactiae and to perform constant epidemiological surveillance to detect changes in the sensitivity profiles of the isolates.
Introducción. Streptococcus agalactiae es el principal agente etiológico causante de infección invasiva del recién nacido con cuadros clínicos que pueden cursar con septicemia, neumonía o meningitis con prevalencias hasta del 50 % a nivel mundial, donde se viene presentando un incremento en su resistencia antibiótica. Objetivo. Estimar la prevalencia de colonización vaginorrectal por S. agalactiae y su perfil de sensibilidad, en mujeres embarazadas atendidas en un hospital de tercer nivel. Materiales y métodos. Se muestrearon 121 mujeres gestantes mediante hisopado vaginal y rectal. Los cultivos se desarrollaron siguiendo la metodología recomendada por los Centers for Disease Control and Prevention (CDC) y se agregó el agar chromID Strepto B. Las colonias sugestivas se identificaron bioquímicamente y se determinaron los perfiles de sensibilidad según el Clinical and Laboratory Standards Institute (CLSI). Resultados. La prevalencia de colonización por S. agalactiae en las mujeres gestantes fue del 20,66 %. Se obtuvieron 40 aislamientos del total de muestras analizadas, de los cuales, el 12,5 % no presentó sensibilidad a la penicilina. La sensibilidad a la levofloxacina, la clindamicina y la eritromicina fue de 100 %, 92,5 % y 87,5 %, respectivamente; no se encontró sensibilidad a la tetraciclina. El fenotipo iMLSB se encontró en tres y, el M, en dos de los 40 aislamientos. Conclusiones. La prevalencia de colonización vaginorrectal por S. agalactiae en la población de estudio, fue de 20,66 %. Se obtuvieron aislamientos no sensibles a la penicilina, y con resistencia a los macrólidos y las lincosamidas mediante el método de Kirby-Bauer. Por ello, es importante la búsqueda activa en las mujeres gestantes colonizadas por estreptococos del grupo B y la vigilancia epidemiológica constante para detectar cambios en los perfiles de sensibilidad de los aislamientos.
Subject(s)
Rectum/microbiology , Streptococcus agalactiae/isolation & purification , Vagina/microbiology , Adult , Colombia , Cross-Sectional Studies , Drug Resistance, Bacterial , Female , Humans , Perineum/microbiology , Pregnancy , Pregnancy Trimester, Third , Streptococcus agalactiae/drug effects , Tertiary Care Centers , Young AdultABSTRACT
OBJECTIVES: Assess Staphylococcus aureus (S. aureus) colonization in healthy Australian adults receiving an investigational S. aureus 3-antigen vaccine (SA3Ag). METHODS: In this phase 1, double-blind, sponsor-unblinded study, participants were randomized to receive a single dose (1 of 3 dose levels) of SA3Ag or placebo and a booster dose or placebo at 6 months. S. aureus isolates from nasal, perineal, and oropharyngeal swabs before and through 12 months post-vaccination were identified. RESULTS: Baseline S. aureus colonization prevalence was 30.6% (any site), with nasal carriage (27.0%) more common than oropharyngeal/perineal (3.2% each). Following initial vaccination (low-dose: 102; mid-dose: 101; high-dose: 101; placebo: 102) and booster (low-dose: 45; mid-dose: 44; high-dose: 27; placebo: 181), placebo and SA3Ag groups showed similar S. aureus carriage through 12 months. Most colonized participants (74.0%) were colonized by single spa types. Placebo and SA3Ag groups had similar persistence of colonization, with 19.6-30.7% due to single spa types. Acquisition was observed in mid- and high-dose recipients (â¼20%) and low-dose and placebo recipients (â¼12%). Vaccination resulted in substantial increases in antibodies to all 3 antigens, irrespective of carriage status. CONCLUSIONS: Based on descriptive analyses of this small study, SA3Ag vaccination did not impact S. aureus acquisition or carriage. Carriage status did not impact antibody responses to SA3Ag.
Subject(s)
Carrier State/epidemiology , Carrier State/prevention & control , Staphylococcal Infections/epidemiology , Staphylococcal Infections/prevention & control , Staphylococcal Vaccines/immunology , Staphylococcus aureus/classification , Staphylococcus aureus/isolation & purification , Adolescent , Adult , Aged , Aged, 80 and over , Antigens, Bacterial/administration & dosage , Antigens, Bacterial/immunology , Australia , Carrier State/microbiology , Double-Blind Method , Healthy Volunteers , Humans , Immunization Schedule , Middle Aged , Nasal Mucosa/microbiology , Oropharynx/microbiology , Perineum/microbiology , Placebos/administration & dosage , Prevalence , Staphylococcal Infections/microbiology , Staphylococcal Vaccines/administration & dosage , Staphylococcus aureus/immunology , Treatment Outcome , Vaccines, Subunit/administration & dosage , Vaccines, Subunit/immunology , Young AdultABSTRACT
BACKGROUND: Many data suggest that patients with low rectal adenocarcinoma who achieved ypT0N0 status have improved survival and disease-free survival (DFS) compared to all other stages however only few data are available regarding the specific prognosis factors of this subgroup. This study aimed to evaluate predictive factors for disease free survival after complete pathological response (CPR) in cases of low rectal adenocarcinoma. MATERIALS AND METHODS: From January 2005 to December 2013, all patients with low rectal adenocarcinoma who underwent neoadjuvant chemoradiotherapy followed by total mesorectal excision and achieved CPR were included at 7 Moroccan and 1 Algerian centres. Predictive factors for disease-free survival were analysed by uni and multivariate analysis. RESULTS: Eigthy-four (12.1%) patients achieved a CPR (ypT0N0). Multivariate analysis revealed that both poorly differentiated tumors (OR, 9.23; 95 CI 1.35-62.82; P = 0.023) and the occurrence of perineal sepsis (OR, 13.51; 95 CI 1.96-93.12; P = 0.008) were independently associated with impaired DFS. CONCLUSIONS: Patients with low rectal cancer who exhibited a CPR after neoadjuvant therapy have good prognoses; however, the occurrence of perineal sepsis and/or poor initial differentiation may be associated with impaired DFS in these patients. TRIAL REGISTRATION: The study was retrospectively registered the 28th July 2018 in ClinicalTrials.gov register with the reference NCT03601689.
Subject(s)
Adenocarcinoma/mortality , Adenocarcinoma/therapy , Chemoradiotherapy/mortality , Neoadjuvant Therapy/mortality , Rectal Neoplasms/mortality , Rectal Neoplasms/therapy , Adenocarcinoma/pathology , Adenocarcinoma/surgery , Adult , Aged , Disease-Free Survival , Female , Follow-Up Studies , Humans , Kaplan-Meier Estimate , Male , Middle Aged , Neoplasm Recurrence, Local , Neoplasm Staging , Perineum/microbiology , Prognosis , Rectal Neoplasms/pathology , Rectal Neoplasms/surgery , SepsisABSTRACT
Erysipelas is a common skin infection causing significant morbidity. At present there are no established procedures for bacteriological sampling. Here we investigate the possibility of using cultures for diagnostic purposes by determining the perianal colonization with beta-hemolytic streptococci (BHS) in patients with erysipelas. Patients with erysipelas and a control group of patients with fever without signs of skin infection were prospectively included and cultures for BHS were taken from the tonsils, the perianal area, and wounds. BHS were grouped according to Lancefield antigen, species-determined, and emm-typed. Renewed cultures were taken after four weeks from patients with erysipelas and a positive culture for BHS. 25 patients with erysipelas and 25 with fever were included. In the group with erysipelas, 11 patients (44%) were colonized with BHS, ten patients were colonized in the perianal area, and one patient in the throat. In contrast, only one patient in the control group was colonized (p = 0.005 for difference). All of the patients with erysipelas colonized with BHS had an erythema located to the lower limb. The BHS were then subjected to MALDI-TOF MS and most commonly found to be Streptococcus dysgalactiae. Renewed cultures were taken from nine of the 11 patients with BHS and three of these were still colonized. Streptococcus dysgalactiae colonizes the perianal area in a substantial proportion of patients with erysipelas. The possibility of using cultures from this area as a diagnostic method in patients with erysipelas seems promising.
Subject(s)
Carrier State/microbiology , Erysipelas/microbiology , Streptococcus/isolation & purification , Adolescent , Adult , Aged , Aged, 80 and over , Anal Canal/microbiology , Carrier State/epidemiology , Female , Humans , Male , Middle Aged , Palatine Tonsil/microbiology , Perineum/microbiology , Prevalence , Prospective Studies , Wounds and Injuries/microbiology , Young AdultABSTRACT
BACKGROUND: Early life microflora is an important determinant of immune and metabolic development and may have lasting consequences. However, the mode of delivery and the effect of povidone iodine disinfection on neonatal oral microflora colonization are still unclear. The objective of the study was to understand the effects of the use of polyvidone iodine on infant's oral microflora after transvaginal examination during delivery, provided data support for the establishment of neonatal oral microflora health. METHODS: A total of 20 cases of full-term neonatal delivered in October 2017 in Shenzhen Bao'an Maternity and Child Health Hospital through vaginal delivery. These neonates were randomly divided into two groups, the conventional disinfection group and the non-disinfection group. Simultaneously, 10 infants with elective cesarean section were taken as comparison. With Illumina MiSeq platform, 16S rRNA V3-V4 sequencing method was used to analyze bacterial DNA of oral secretions. RESULTS: At the phylum level, compared to the non-disinfection group, higher relative abundance of Bacteroidetes and Proteobacteria, and lower proportion of Firmicutes were observed in the cesarean section group and the disinfection group. As main composition of phylum Firmicutes, genus Lactobacillus presented extremely low in the cesarean section group and the disinfection group, whereas it was the absolute dominant bacteria in the non-disinfection group. Compared with the caesarean section group, only Lactobacillus increased in majority of the non-disinfection group. There was no increase in Lactobacillus in the disinfection group, but Prevotella, Escherichia-Shigella, Staphyloccus, and Klebsiella increased significantly. Through KEGG pathway analysis, we found that there were more harmful pathways such as staphylococcus aureus infection, viral myocarditis and sporulation in the disinfection group. CONCLUSIONS: The mode of delivery affects the infant's Lactobacillus obtained from the mother. Moreover, vulvar disinfection played an important part in the colonization of neonatal oral microbiota. And the impact of the first oral colonizers on infant health needs further follow-up investigations.
Subject(s)
Bacteria/isolation & purification , Delivery, Obstetric/methods , Disinfection , Mouth/microbiology , Perineum/microbiology , Adult , Anti-Infective Agents, Local , Bacteria/genetics , Bacteroidetes/isolation & purification , Cesarean Section , DNA, Bacterial/analysis , Escherichia/isolation & purification , Female , Humans , Infant, Newborn , Klebsiella/isolation & purification , Lactobacillus/isolation & purification , Male , Povidone-Iodine , Prevotella/isolation & purification , Proteobacteria/isolation & purification , Staphylococcus/isolation & purification , Vagina , Young AdultABSTRACT
Necrotising fasciitis (NF) is a potentially lethal spread of infection that is uncommonly seen within the province of surgery. Seen mostly in the extremities and the perineal regions, it has been reported rarely to involve the retroperitoneal space and presents with a spectrum of symptoms and signs as such. Literature supports classification of NF based on the microbes involved. Irrespective of the aetiology and the causative organism, NF remains a serious surgical emergency with high morbidity and mortality not only associated with the disease process itself, but also with the extensive surgical debridement it requires in its management along with antimicrobial administration. We present a case of such an infection found in the retroperitoneal space secondary to a perineal infection forming a rare presentation of this deadly process, and how it was successfully managed secondary to timely surgical intervention.
Subject(s)
Fasciitis, Necrotizing/diagnostic imaging , Perineum/microbiology , Retroperitoneal Space/microbiology , Administration, Intravenous , Aged , Anti-Bacterial Agents/administration & dosage , Anti-Bacterial Agents/therapeutic use , Colostomy/methods , Corynebacterium/isolation & purification , Debridement , Fasciitis, Necrotizing/drug therapy , Fasciitis, Necrotizing/surgery , Female , Humans , Jejunostomy/methods , Laparotomy/methods , Perineum/diagnostic imaging , Perineum/pathology , Perineum/surgery , Retroperitoneal Space/pathology , Tomography, X-Ray Computed/methods , Treatment OutcomeABSTRACT
Background: Erysipelas and cellulitis are usually caused by betahaemolytic streptococci but the aetiology is often difficult to verify in clinical practice. Methods: Patients with erysipelas or cellulitis were analysed for betahaemolytic streptococci in samples from multiple body sites, including the perineum and the anal canal, during the acute episode and at follow up. Healthy control persons were sampled from the same sites. Results: Betahaemolytic streptococci group A, C or G were identified in 23/28 (82%) patients, most commonly group G. A wound or ulcer, present in 16/28 (57%), was colonized in 8/16 (50%). The perineum and anal canal were colonized in 11/28 (39%) and 10/28 (36%), respectively. At follow-up after about 4 weeks, only 4/28 (14%) were colonized (p<.001). In 39 healthy control persons, no betahaemolytic streptococci group A were found, groups C or G were found in 4/39 (10%). Group B streptococci were more often identified in controls, than in patients,12/39 (31%). Conclusions: Acute episodes of erysipelas or cellulitis are associated with colonization of betahaemolytic streptococci at multiple sites including the perineum and anal canal, in particular serogroup G. This may be important for choice of primary antibiotic therapy and possibilities for prevention of relapses.
Subject(s)
Anal Canal/microbiology , Cellulitis/microbiology , Erysipelas/microbiology , Perineum/microbiology , Streptococcal Infections/microbiology , Streptococcus agalactiae/isolation & purification , Streptococcus pyogenes/isolation & purification , Viridans Streptococci/isolation & purification , Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Streptococcus agalactiae/classification , Streptococcus pyogenes/classification , Viridans Streptococci/classification , Young AdultABSTRACT
In Denmark, eradication treatment is recommended for methicillin-resistant Staphylococcus aureus (MRSA) carriers. Here, we analyze factors associated with eradication outcome. MRSA carriers referred to the MRSA Knowledge Center at Hvidovre Hospital in 2013 were included. Carriers were sampled from nose, throat, and perineum. Eradication regimen was 5 days of mupirocin nasal ointment and chlorhexidine whole-body wash. Oral antibiotics were sometimes added. Factors associated with eradication after the first eradication attempt were analyzed by logistic regression and expressed as odds ratio (OR) with 95% confidence interval (95% CI). Of 164 individuals, 143 completed 1- and 6-month follow-up after 1st treatment. Eradication was achieved in 63 (38.4%) patients after one treatment and 101 (61.6%) individuals became MRSA free after up to 4 eradication treatments. Throat carriage was associated with a higher failure rate (OR 0.29 (0.10-0.80)), while the presence of Panton-Valentine leukocidin (PVL) genes (37%) was associated with higher success rate (OR 3.52 (1.44-8.57)). Other factors analyzed were not significantly associated with eradication outcome. None of the 26 patients lost to follow-up developed later MRSA infections. This study estimates the efficacy of treatment of MRSA carriage with an eradication rate of 38.4% after the first treatment and a total eradication rate of 61.6% after several treatments. Throat carriers had a lower eradication success rate. Adding oral antibiotics to the first treatment did not increase success. The finding of a significant higher success rate when having a PVL-positive clone should be further investigated.
Subject(s)
Bacterial Toxins/genetics , Carrier State/microbiology , Exotoxins/genetics , Leukocidins/genetics , Methicillin-Resistant Staphylococcus aureus/drug effects , Pharynx/microbiology , Staphylococcal Infections/drug therapy , Adolescent , Adult , Aged , Aged, 80 and over , Anti-Bacterial Agents/therapeutic use , Child , Child, Preschool , Denmark , Female , Humans , Male , Methicillin-Resistant Staphylococcus aureus/genetics , Middle Aged , Nose/microbiology , Perineum/microbiology , Retrospective Studies , Staphylococcal Infections/prevention & control , Treatment Outcome , Whole Genome Sequencing , Young AdultABSTRACT
BACKGROUND: Health care workers (HCWs) are significant vectors for transmission of multidrug-resistant organisms among patients in intensive care units (ICUs). We studied ICU patients on contact precautions, colonized with vancomycin-resistant Enterococcus (VRE), to assess whether bacterial burden is associated with transmission to HCWs' gloves or gowns, a surrogate outcome for transmission to subsequent patients. METHODS: From this prospective cohort study, we analyzed 96 VRE-colonized ICU patients and 5 HCWs per patient. We obtained samples from patients' perianal area, skin, and stool to assess bacterial burden and cultured HCWs' gloves and gowns for VRE after patient care. RESULTS: Seventy-one of 479 (15%) HCW-patient interactions led to contamination of HCWs' gloves or gowns with VRE. HCW contamination was associated with VRE burden on the perianal swab (odds ratio [OR], 1.37; 95% confidence interval [CI], 1.19, 1.57), skin swabs (OR, 2.14; 95% CI, 1.51, 3.02), and in stool (OR, 1.95; 95% CI, 1.39, 2.72). Compared with colonization with Enterococcus faecalis, colonization with Enterococcus faecium was associated with higher bacterial burden and higher odds of transmission to HCWs. CONCLUSIONS: We show that ICU patients with higher bacterial burden are more likely to transmit VRE to HCWs. These findings have implications for VRE decolonization and other infection control interventions.