ABSTRACT
A 20-year-old female resident of Beijing intended to consume the eggs of the parasitic worm, Taenia saginata, for weight loss; however, she apparently inadvertently ingested Taenia solium (pork tapeworm) eggs, which resulted in disseminated cysticercosis. Cysticerci developed in the brain, tongue, muscles, liver, peritoneum, and subcutaneous tissues. She was administered oral albendazole and praziquantel. After four 10-day courses of treatment, most of the cysts disappeared and she recovered. After 3 years, the patient remains in good health.
Subject(s)
Anthelmintics/therapeutic use , Brain/pathology , Cysticercosis/pathology , Taenia solium/pathogenicity , Tongue/pathology , Albendazole/therapeutic use , Animals , Brain/diagnostic imaging , Brain/parasitology , Cysticercosis/diagnostic imaging , Cysticercosis/drug therapy , Cysticercosis/parasitology , Female , Humans , Liver/diagnostic imaging , Liver/parasitology , Liver/pathology , Muscles/diagnostic imaging , Muscles/parasitology , Muscles/pathology , Peritoneum/diagnostic imaging , Peritoneum/parasitology , Peritoneum/pathology , Praziquantel/therapeutic use , Subcutaneous Tissue/diagnostic imaging , Subcutaneous Tissue/parasitology , Subcutaneous Tissue/pathology , Taenia saginata , Taenia solium/growth & development , Tongue/diagnostic imaging , Tongue/parasitology , Treatment Outcome , Weight Loss , Young Adult , Zygote/growth & development , Zygote/pathologyABSTRACT
Canine peritoneal larval cestodiasis (CPLC) is a little-known parasitological infestation of the peritoneal cavity of wild and domestic carnivores with Mesocestoides spp. larvae. While adult Mesocestoides tapeworms reside within the small intestine, the larvae occasionally penetrate the host's intestinal wall, causing a potentially life-threatening peritonitis. Severity of infection as well as the host response influences the prognosis significantly, and early diagnosis and treatment are essential. However, due to the lack of specific symptoms, this condition is underdiagnosed and, furthermore, no clear effective treatment has yet been described. The aim of this study is therefore to report two clinical cases of CPLC in dogs and to illustrate their clinical presentation and follow-up to serve as a reference for clinicians and researchers alike. Both animals were presented with abdominal distention as their main complaint. They underwent clinical examination, abdominal ultrasonography, abdominocentesis, and laparotomy followed by biochemical, cytological, parasitological, and molecular examination of the collected samples. After surgical lavage, the dogs received anthelmintic treatment with either fenbendazole (FBZ) or praziquantel (PZQ). Overall, timely and prolonged administration of high doses of FBZ seems to be the most effective treatment method. Irrespective, to date, no treatment capable of complete eradication of the infection and prevention of recurrence of disease has been found. In conclusion, further investigation into appropriate treatment plans as well as diagnostic development is needed.
Subject(s)
Cestode Infections/parasitology , Dog Diseases/parasitology , Mesocestoides/isolation & purification , Peritoneum/parasitology , Animals , Anthelmintics/therapeutic use , Cestode Infections/diagnosis , Cestode Infections/drug therapy , Dog Diseases/diagnosis , Dog Diseases/therapy , Dogs , Female , Follow-Up Studies , Larva/drug effects , Larva/growth & development , Male , Mesocestoides/drug effects , Mesocestoides/growth & development , Treatment OutcomeABSTRACT
BACKGROUND: Here, Mesocestoides (M.) vogae infection in mice is proposed as a suitable experimental model for studying the immunity in the peritoneal cavity of mice. METHODS: To investigate the kinetics of immune parameters in M. vogae-infected mice, we detected, using flow cytometry, the expression of selected lymphoid and myeloid markers within the peritoneal cell population at day 0, 3, 6, 10, 14, 19, 25, 30 and 35 post-infection. Then, using ELISA, we analyzed the cytokine IFN-γ, TGF-ß, IL-4 and IL-10 responses and the levels of anti-M. vogae IgG and IgM antibodies in the peritoneal lavage fluid. Cells isolated from the peritoneal cavity were subjected to further molecular analysis. To assess cell activation, peritoneal cells were exposed to LPS, and culture supernatants were collected and assayed for the level of cytokines and production of nitrite. Ly6C+ and Ly6G+ cells were isolated using MACS from the peritoneal cells at day 35 post-infection. Both MACS-isolated subsets were co-cultured with preactivated T cells to measure their suppressive capacity. Next, the role of parasite excretory-secretory antigens in induction of CD11b+ myeloid cells with the suppressive phenotype and the production of IL-10 was examined. RESULTS: In the peritoneal cavity an initial increase of CD11b+Gr-1+F4/80highMHC IIhigh cells, NK, NKT cells and CD8+ cytotoxic T cells was observed in the first week of infection. At day 14 post-infection, an increase in the number of myeloid CD11b+Gr-1+ cells was detected, and most of this cell population expressed low levels of F4/80 and MHC II in later stages of infection, suggesting the impairment of antigen-presenting cell functions, probably through the excretory-secretory molecules. Moreover, we confirmed that peritoneal Gr1+ cells (Ly6C+ and Ly6G+ population) are phenotypically and functionally consistent with myeloid-derived suppressor cells. Metacestode infection elicited high levels of IL-10 and upregulated STAT-3 in peritoneal cells. A higher level of IgM suggests that this isotype may be predominant and is involved in the host protection. CONCLUSIONS: Mesocestoides vogae tetrathyridia induced the recruitment of immunosuppressive cell subsets, which may play a key role in the downregulation of immune response in long-term parasitic diseases, and excretory-secretory antigens seem to be the main regulatory factor.
Subject(s)
Cestode Infections/immunology , Immunity, Cellular , Immunity, Humoral , Mesocestoides/immunology , Peritoneum/immunology , Animals , Cytokines , Disease Models, Animal , Flow Cytometry , Male , Mesocestoides/pathogenicity , Mice , Mice, Inbred BALB C , Peritoneum/cytology , Peritoneum/parasitologyABSTRACT
The helminth parasite Fasciola hepatica modulates the host immune response at early stages of infection (Rodríguez et al., PLoS Negl Trop Dis 9:e0004234, 2015; Vukman et al., J Immunol 190:2873-2879, 2013). Nevertheless, little is known about the cell composition of the peritoneal fluid at these early stages of infection.In this chapter, we describe a method to perform peritoneal lavages and to recover peritoneal fluid from sheep experimentally infected and noninfected with F. hepatica at early stages of infection. In addition, with the aim to characterize the peritoneal fluid immune cell phenotype, we describe a procedure to obtain the total leukocyte count, the differential leukocyte count and the preparation and storage of peritoneal fluid smears, together with the application of an immunocytochemical technique and an automatic method to count the immunoreactive cells. Finally, the present protocol describes the evaluation of the gross and the histopathological lesions together with the immunohistochemical analysis of the hepatic tissue.
Subject(s)
Ascitic Fluid/immunology , Fasciola hepatica/immunology , Fascioliasis/immunology , Liver/immunology , Microscopy/methods , Peritoneal Lavage/methods , Peritoneum/immunology , Animals , Antibodies, Helminth/immunology , Ascitic Fluid/parasitology , Fascioliasis/parasitology , Immunohistochemistry/methods , Leukocyte Count/methods , Liver/parasitology , Peritoneal Cavity/parasitology , Peritoneum/parasitology , Sheep/immunology , Sheep/parasitology , Sheep Diseases/immunology , Sheep Diseases/parasitologyABSTRACT
A 7-month-old female mixed breed foal with a 2-day history of recumbency and inability to open its mouth convulsed acutely and died and was submitted for necropsy examination. The foal was thin and large patches of haemorrhage were present throughout the peritoneal wall, the diaphragmatic surfaces and the retroperitoneum. Numerous nematode larvae were visible on the serosal surfaces and penetrated and embedded into the subserosa associated with the haemorrhages. The dorsal portion of the abdominal diaphragm had a partial tear and large numbers of nematodes were within the muscle fibres. Histologically, the larvae had a smooth cuticle, polymyarian/coelomyarian musculature and multinucleated intestinal cells, and were typically surrounded by haemorrhage, neutrophils, dense fibrovascular connective tissue and rare multinucleated giant cells. Parasitological examination identified the larvae as Strongylus edentatus based on the morphology of the buccal capsule. Additionally, there was severe muscle necrosis of the tongue and liver tissue analysis detected selenium deficiency. S. edentatus infections are uncommon in California, USA, and are typically non-lethal. In this case, the selenium deficiency may have led to immunosuppression, resulting in the hyperinfection with S. edentatus, and to the muscle damage and tear of the diaphragm. Although ivermectin treatment was indicated in the history, inadequate deworming or anthelmintic resistance may have played a role in the severity of infection.
Subject(s)
Horse Diseases/pathology , Horse Diseases/parasitology , Strongyle Infections, Equine/pathology , Strongylus , Animals , Female , Horses , Larva/parasitology , Larva/pathogenicity , Liver/chemistry , Muscles/parasitology , Muscles/pathology , Peritoneum/parasitology , Peritoneum/pathology , Selenium/analysis , Strongylus/isolation & purificationABSTRACT
Immune signatures of sheep acutely-infected with Fasciola hepatica, an important pathogen of livestock and humans were analysed within the peritoneal compartment to investigate early infection. Within the peritoneum, F. hepatica antibodies coincided with an intense innate and adaptive cellular immune response, with infiltrating leukocytes and a marked eosinophilia (49%). However, while cytokine qPCR analysis revealed IL-10, IL-12, IL-13, IL-23 and TGFß were elevated, these were not statistically different at 18 days post-infection compared to uninfected animals indicating that the immune response is muted and not yet skewed to a Th2 type response that is associated with chronic disease. Proteomic analysis of the peritoneal fluid identified infection-related proteins, including several structural proteins derived from the liver extracellular matrix, connective tissue and epithelium, and proteins related to the immune system. Periostin and vascular cell adhesion protein 1 (VCAM-1), molecules that mediate leukocyte infiltration and are associated with inflammatory disorders involving marked eosinophilia (e.g. asthma), were particularly elevated in the peritoneum. Immuno-histochemical studies indicated that the source of periostin and VCAM-1 was the inflamed sheep liver tissue. This study has revealed previously unknown aspects of the immunology and pathogenesis associated with acute fascioliasis in the peritoneum and liver.
Subject(s)
Fasciola hepatica/immunology , Fascioliasis/veterinary , Peritoneum/immunology , Peritoneum/parasitology , Sheep Diseases/immunology , Sheep Diseases/parasitology , Animals , Biomarkers , Biopsy , Cytokines/blood , Cytokines/metabolism , Extracellular Matrix/metabolism , Gene Expression Profiling , Inflammation Mediators , Leukocyte Count , Liver/immunology , Liver/metabolism , Liver/parasitology , Liver/pathology , Sheep , Sheep Diseases/metabolism , Sheep Diseases/pathologyABSTRACT
Cystic echinococcosis is a widespread zoonotic parasitic disease especially in Tunisia which is one of the most endemic countries in the Mediterranean area. The etiological agent, Echinococcus granulosus sensu lato, implies dogs and other canids as definitive hosts and different herbivore species as intermediate hosts. Human contamination occurs during the consumption of parasite eggs passed in the environment through canid feces. Hydatid cysts coming from a child operated for multiple echinococcosis were collected and analyzed in order to genotype and to obtain some epidemiological molecular information. Three targets, ribosomal DNA ITS1 fragment, NADH dehydrogenase subunit 1 (nad1), and mitochondrial cytochrome c oxydase subunit 1 (CO1) genes, were amplified and analyzed by RFLP and sequencing approach. This study presents the first worldwide report in human of a simultaneous infection with Echinococcus granulosus sensu stricto (genotype G1) and Echinococcus canadensis (genotype G6) species. This is also the first report of the presence of E. canadensis in the Tunisian population which argues in favor of a greater importance of this species in human infestation in Tunisia than previously believed.
Subject(s)
Echinococcosis/parasitology , Echinococcus granulosus/classification , Albendazole/therapeutic use , Animals , Anticestodal Agents/therapeutic use , Canidae/parasitology , Child , DNA, Helminth/chemistry , DNA, Helminth/isolation & purification , DNA, Intergenic/chemistry , DNA, Intergenic/genetics , Dogs , Echinococcosis/drug therapy , Echinococcosis/surgery , Echinococcus granulosus/genetics , Echinococcus granulosus/pathogenicity , Electron Transport Complex IV/genetics , Genotype , Humans , Liver/parasitology , Liver/surgery , Male , Mitochondria/genetics , Molecular Epidemiology , Oxidoreductases/genetics , Peritoneum/parasitology , Peritoneum/surgery , Polymorphism, Restriction Fragment Length , Tunisia , Zoonoses/parasitologyABSTRACT
Extraintestinal oxyuriasis, in our experience with three affected women of fertile age, presented itself as a solitary fibrotic nodular lesion, with a varying location. The sites of location were: parietal peritoneum, serous surface of the uterus and wall of the uterine tube. The size of the nodules was 5 to 10 mm. Histologically, the lesions were hypocellular fibrotic nodules with a variable amount of neutrophils and amorphous eosinophilic material in the center, harbouring eggs of the parasite and remnants of pinworm cuticle. All three lesions were asymptomatic, only being discovered incidentally during the operations for unrelated conditions. Their peroperative recovery by a surgeon did not alter the course of surgery. These findings document the ability of pinworms to migrate into the abdominal cavity via the female genital tract.
Subject(s)
Fallopian Tubes/parasitology , Oxyuriasis/pathology , Peritoneum/parasitology , Uterus/parasitology , Adult , Female , Humans , Incidental FindingsABSTRACT
Canine peritoneal larval cestodiasis (CPLC) is an unusual parasitic disease in dogs that is caused by asexual proliferation of larval Mesocestoides. A 12 year-old spayed Shetland sheepdog with abdominal distension was referred to the Animal Medical Center at Nihon University, Japan. The presence of ascites was confirmed by abdominal ultrasonography and X-ray imaging. In addition, a number of parasites were observed in the ascitic fluid collected by abdominal paracentesis. Each of the whitish colored parasites was less than 1mm in size. The parasites were morphologically identified as Mesocestoides sp. tetrathyridia. The parasites had four suckers and calcareous corpuscles, but no hooks or rostellum. Mitochondrial (mt) 12S rDNA and mt cytochrome c oxidase subunit 1 DNA amplified from the tetrathyridia were used for molecular identification to species level. DNA sequence analysis showed that the tetrathyridia shared more than 99% identity with M. vogae (syn. M. corti) for each gene. The patient was treated with a standard dose (5mg/kg) of praziquantel, which was administered subcutaneously twice at an interval of 14 days. This resulted in successful deworming. This is the first case that CPLC was diagnosed in a dog that had never been taken outside of Japan, indicating that M. vogae is distributed in this country.
Subject(s)
Cestode Infections/veterinary , Dog Diseases/diagnosis , Dog Diseases/parasitology , Mesocestoides/physiology , Peritoneum/parasitology , Animals , Cestode Infections/diagnosis , Cestode Infections/drug therapy , Cestode Infections/parasitology , Dog Diseases/drug therapy , Dogs , Electron Transport Complex IV/genetics , Female , Japan , Larva , Mesocestoides/genetics , Praziquantel/therapeutic use , RNA, Ribosomal/genetics , Sequence Homology, Nucleic Acid , Treatment OutcomeABSTRACT
PURPOSE: To describe peritoneal manifestations of fascioliasis on CT. MATERIALS AND METHODS: We reviewed CT images in 31 patients with fascioliasis confirmed by enzyme-linked immunosorbent assay (ELISA) (n = 24) or surgery (n = 7). Image analyses were performed to identify hepatic, biliary, and peritoneal abnormalities. RESULTS: Hepatic abnormalities were seen in 28 (90.3 %) of the 31 patients. The most common finding was caves sign, which was present in 25 (80.1 %) patients. Three patients (9.7 %) presented with biliary abnormalities exhibiting dilatation and enhancing wall thickening of the bile duct, wall thickening of the gallbladder, and elongated structures in the bile duct or gallbladder. Peritoneal abnormalities were seen in 14 (45.2 %) of the 31 patients. The most common peritoneal abnormality was mesenteric or omental infiltration, which was seen in 9 (29.0 %) patients. Other peritoneal findings included lymph node enlargement (n = 7), ascites (n = 7), thickening of ligamentum teres (n = 2), and peritoneal mass (n = 2). CONCLUSION: Peritoneal manifestations of fascioliasis are relatively common, and CT findings include mesenteric or omental infiltration, lymph node enlargement, ascites, thickening of the ligamentum teres, and peritoneal masses.
Subject(s)
Fascioliasis/diagnostic imaging , Peritoneum/diagnostic imaging , Peritoneum/parasitology , Tomography, X-Ray Computed , Adult , Aged , Aged, 80 and over , Biliary Tract/diagnostic imaging , Biliary Tract/parasitology , Cholecystography , Fascioliasis/complications , Female , Gallbladder/parasitology , Humans , Liver/diagnostic imaging , Liver/parasitology , Male , Middle Aged , Peritoneal Diseases/diagnostic imaging , Peritoneal Diseases/etiology , Peritoneal Diseases/parasitology , Tomography, X-Ray Computed/methodsABSTRACT
Changes and local immune response were evaluated in the peritoneal cell populations, duodenal lamina propria and liver from goats immunized with recombinant glutathione transferase sigma class (rFhGST-S1) during early stages of infection with Fasciola hepatica. Group 1 (n=7) was unimmunized and uninfected; group 2 (n=10) was immunized with adjuvant Quil A and infected; group 3 (n=10) was immunised with rFhGST-S1 and infected. Three goats from each group were killed at 7-9 days post-infection (dpi) to evaluate early changes and immune response. The remaining goats were killed at 15 weeks post-infection (wpi). rFhGST-S1 vaccination induced variable response: three goats showed low fluke burden at 15 wpi and two goats showed low hepatic damage at early infection stages. This response was associated to a severe infiltrate of eosinophils in peritoneal fluid and hepatic necrotic foci, high iNOS expression in peritoneal cells and abundant infiltrate of eosinophils surrounding hepatic migrating flukes. T lymphocyte subsets were found in the vicinity of necrotic areas but they were absent in the vicinity of migrating larvae. No significant variation for T cell subsets, except for CD4 and γδ T lymphocytes, that were higher in the Quil A group compared to the rFhGST-S1 group. Expression of IL4 and IFN-γ in the hepatic inflammatory infiltrates was very occasional.
Subject(s)
Fasciola hepatica/immunology , Fascioliasis/veterinary , Goat Diseases/parasitology , Liver/pathology , Peritoneum/pathology , Vaccines/immunology , Adjuvants, Immunologic/therapeutic use , Animals , Fascioliasis/immunology , Fascioliasis/parasitology , Fascioliasis/pathology , Fascioliasis/prevention & control , Glutathione Transferase/genetics , Glutathione Transferase/immunology , Goat Diseases/immunology , Goat Diseases/pathology , Goat Diseases/prevention & control , Goats/immunology , Goats/parasitology , Immunity, Cellular/immunology , Immunity, Humoral/immunology , Liver/parasitology , Male , Peritoneum/parasitology , Quillaja Saponins , Saponins/therapeutic use , Vaccines/therapeutic use , Vaccines, Synthetic/immunology , Vaccines, Synthetic/therapeutic useABSTRACT
Toxoplasma gondii is an obligate intracellular protozoa parasite that causes the disease toxoplasmosis. It resides within host cells in a parasitophorous vacuole distinct from the host cell endocytic system. T. gondii was used as a model to investigate how obligate intracellular parasites alter their gene expression in response to the host immune response during infection compared to growth in host cells in vitro. While bacterial pathogens clearly alter gene expression to adapt to the host environment during infection, the degree to which the external environment affects gene expression by obligate intracellular pathogens sequestered within host cells is less clear. The global transcriptome of T. gondii was analyzed in vivo in the presence and absence of the IFN-γ-dependent host innate immune response. The parasites' in vivo transcriptome was also compared to its transcriptome in vitro in fibroblast cells. Our results indicate that the parasite transcriptome is significantly altered during in vivo infection in the presence, but not absence, of IFN-γ-dependent immunity compared with fibroblasts infected in vitro. Many of the parasite genes increased in vivo appear to be common to an early general stress response by the parasite; surprisingly putative oocyst stage specific genes were also disproportionately increased during infection.
Subject(s)
Genes, Protozoan , Toxoplasma/genetics , Toxoplasmosis/immunology , Transcriptome , Animals , Cells, Cultured , Fibroblasts/immunology , Fibroblasts/metabolism , Fibroblasts/parasitology , Gene Expression Regulation , Host-Parasite Interactions , Humans , Immunity, Innate , Interferon-gamma/genetics , Interferon-gamma/metabolism , Interferon-gamma/physiology , Mice , Mice, Knockout , Oligonucleotide Array Sequence Analysis , Oocysts/metabolism , Peritoneum/immunology , Peritoneum/metabolism , Peritoneum/parasitology , Signal Transduction/genetics , Toxoplasma/metabolism , Toxoplasma/physiology , Toxoplasmosis/parasitologyABSTRACT
Mammals are serially infected with a variety of microorganisms, including bacteria and parasites. Each infection reprograms the immune system's responses to re-exposure and potentially alters responses to first-time infection by different microorganisms. To examine whether infection with a metazoan parasite modulates host responses to subsequent bacterial infection, mice were infected with the hookworm-like intestinal nematode Nippostrongylus brasiliensis, followed in 2-4 weeks by peritoneal injection of the pathogenic bacterium Klebsiella pneumoniae. Survival from Klebsiella peritonitis two weeks after parasite infection was better in Nippostrongylus-infected animals than in unparasitized mice, with Nippostrongylus-infected mice having fewer peritoneal bacteria, more neutrophils, and higher levels of protective interleukin 6. The improved survival of Nippostrongylus-infected mice depends on IL-4 because the survival benefit is lost in mice lacking IL-4. Because mast cells protect mice from Klebsiella peritonitis, we examined responses in mast cell-deficient Kit(W-sh)/Kit(W-sh) mice, in which parasitosis failed to improve survival from Klebsiella peritonitis. However, adoptive transfer of cultured mast cells to Kit(W-sh)/Kit(W-sh) mice restored survival benefits of parasitosis. These results show that recent infection with Nippostrongylus brasiliensis protects mice from Klebsiella peritonitis by modulating mast cell contributions to host defense, and suggest more generally that parasitosis can yield survival advantages to a bacterially infected host.
Subject(s)
Klebsiella Infections/complications , Klebsiella pneumoniae/pathogenicity , Mast Cells/immunology , Nippostrongylus/pathogenicity , Peritonitis/immunology , Peritonitis/parasitology , Sepsis/complications , Animals , Interleukin-6/metabolism , Intestines/microbiology , Intestines/parasitology , Klebsiella pneumoniae/immunology , Likelihood Functions , Mast Cells/microbiology , Mast Cells/parasitology , Mice , Neutrophil Infiltration , Peritoneum/immunology , Peritoneum/microbiology , Peritoneum/parasitology , Peritonitis/complications , Peritonitis/metabolism , Survival AnalysisABSTRACT
As the main treatment for this infection is surgery, the surgery team personnel are at the risk of the protoscoleces released from the hydatid cysts (HC) of patients. The first goal of this study was to determine the probability of the production of ocular HC in mice due to the fluid of the aspirated protoscoleces from the sheep liver with HC. The second goal of this study was to produce HC in the peritoneum and liver, in order to gather more information for future studies on hepatic and peritoneal HC treatment procedures. For the first goal of this study, different concentrations of protoscoleces were prepared and injected into the eyes of 60 mice. After 20 weeks, 10 of the 60 mice of this group died. The remaining 50 mice were examined by a surgeon under the anesthesia. There weren't any symptoms of HC in the eyes and around it. For the second goal, 39 new mice were separated into three sub groups and 0.5 ml of protoscolex solution was injected intraperitoneally. After 20 weeks, they were anesthetized and their peritoneum, intestines and liver were examined. HC was seen in the peritoneum and liver of 6 mice.
Subject(s)
Echinococcosis/transmission , Echinococcus/growth & development , Eye/parasitology , Liver/parasitology , Peritoneum/parasitology , Animals , Echinococcosis/surgery , Echinococcus/isolation & purification , General Surgery , Lung/parasitology , Mice , Mice, Inbred BALB C , Occupational Diseases/parasitology , SheepABSTRACT
The main aim of this work was to assign the cuticular lipids identified in a parasitic nematode and to distinguish those originating from its host. The hypothesis that long-chained fatty acids and sterols are imported by the parasite in the absence of certain enzymes was also tested. The organisms (Anisakis simplex and Gadus morhua) were extracted in petroleum ether and dichloromethane. Matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF) was used to identify unknown components, and electrospray ionization mass spectrometry (ESI/MS) to verify recognized groups of lipids. The lipid classes identified in the surface layer were free saturated and unsaturated fatty acids, triacylglycerols, sterols and non-polar sphingolipids (ceramides, sphingoid bases). The most abundant fraction consisted of fatty acids. The predominant saturated acids were tetradecanoic acid in the petroleum ether extract of A. simplex, hexadecanoic acid in the dichloromethane extract of A. simplex, and also the polyunsaturated octadecahexaenoic and octadecatrienoic acids in both extracts of the parasitic nematode. The mass spectrum revealed the presence of fatty acids with different numbers of carbons, and with odd and even numbers of unsaturated bonds. The MALDI-TOF mass spectrum also identified triacylglycerols (TAGs). The dominant short-chain TAGs were CoCoCy:(1), CoCoPg and Bu0:0B:(6). The majority of TAGs were found in the ether and dichloromethane extracts of A. simplex. Sterols were the least common class of lipids found in the nematode extracts; most likely, this is the fraction that is entirely incorporated from the host organism because of the parasite's inability to synthesize them. MALDI-TOF also identified non-polar sphingolipids--ceramides and sphingoid bases. The signals due to N-octanoyl-D-erythro-octasphinganine (m/z 288.3) and N-tetranoyl-D-erythro-tetradecasphinganine (m/z 316.4) were dominant on the mass spectra; quite a large number of short-chain non-polar sphingolipids were also identified.
Subject(s)
Anisakiasis/veterinary , Anisakis/chemistry , Fish Diseases/parasitology , Gadus morhua/metabolism , Lipids/analysis , Peritoneum/chemistry , Animals , Anisakiasis/parasitology , Fatty Acids, Nonesterified/analysis , Fatty Acids, Nonesterified/chemistry , Gadus morhua/parasitology , Lipids/classification , Lipids/isolation & purification , Membrane Lipids/analysis , Membrane Lipids/classification , Membrane Lipids/isolation & purification , Peritoneum/parasitology , Spectrometry, Mass, Electrospray Ionization , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Sphingolipids/analysis , Sphingolipids/chemistry , Sterols/analysis , Sterols/chemistry , Triglycerides/analysis , Triglycerides/chemistryABSTRACT
The original description of Henneguya pellis, a myxozoan parasitizing blue catfish Ictalurus furcatus, is supplemented with new data on histopathology, spore morphology, and 18S small subunit (SSU) ribosomal DNA (rDNA) sequence. Plasmodia presented as both internal and external, raised, cyst-like lesions on the body wall of the peritoneal cavity and on the skin. The cysts contained numerous elongate, lanceolate myxospores, flattened parallel to the suture line. The spore body was 14.8 ± 1.1 µm (range 13.0-17.1) long and 4.8 ± 0.8 µm (range 4.0-7.4) wide in frontal view. The caudal appendages were 77.7 ± 8.8 (range 57.4-96.4) in length. There were 2 pyriform polar capsules, unequal in length, with the longer capsule measuring 7.2 ± 0.6 µm (range 6.2-8.4) in length and the shorter capsule measuring 6.5 ± 0.5 µm (range 5.5-8.0). The polar capsules were not significantly different in width, measuring 1.7 ± 0.2 µm (range 1.4-1.9). There were 8 turns in the polar filament coil. The total length of the spore was 92.5 ± 9.2 µm (range 73.3-113.5). Spore morphology and site of development are similar to that of Henneguya sutherlandi from channel catfish; however, 18S rDNA sequence data support previous findings that identify H. pellis and H. sutherlandi as 2 distinct species.
Subject(s)
Fish Diseases/parasitology , Ictaluridae/parasitology , Myxozoa/classification , Parasitic Diseases, Animal/parasitology , RNA, Ribosomal, 18S/genetics , Animals , Base Sequence , DNA Primers/chemistry , DNA, Ribosomal/chemistry , Dermis/parasitology , Molecular Sequence Data , Myxozoa/anatomy & histology , Myxozoa/genetics , Peritoneum/parasitology , Phylogeny , Polymerase Chain Reaction , Sequence AlignmentABSTRACT
The lower incidence of allergy and autoimmune diseases in developing countries has been associated with a high prevalence of parasitic infections. Here we provide direct experimental evidence that parasites can exert bystander immunosuppression of pathogenic T cells that mediate autoimmune diseases. Infection of mice with Fasciola hepatica resulted in recruitment of dendritic cells, macrophages, eosinophils, neutrophils, and CD4(+) T cells into the peritoneal cavity. The dendritic cells and macrophages in infected mice expressed IL-10 and latency-associated peptide, and they had low surface expression of costimulatory molecules and/or MHC class II. Furthermore, most CD4(+) T cells in the peritoneal cavity of infected mice secreted IL-10, but not IFN-gamma or IL-4. There was a less significant expansion of CD4(+)Foxp3(+) T cells. F. hepatica-specific Tr1-type clones generated from infected mice suppressed proliferation and IFN-gamma production by Th1 cells. Infection was associated with suppression of parasite-specific Th1 and Th2 responses, which was reversed in IL-10-defective mice. Infection with F. hepatica also exerted bystander suppression of immune responses to autoantigens and attenuated the clinical signs of experimental autoimmune encephalomyelitis. Protection was associated with suppression of autoantigen-specific IFN-gamma and IL-17 production. The suppression of Th1 and Th17 responses and attenuation of experimental autoimmune encephalomyelitis by F. hepatica was maintained in IL-10(-/-) mice but was reversed by neutralization of TGF-beta in vivo. Our study provides evidence that F. hepatica-induced IL-10 subverts parasite-specific Th1 and Th2 responses, but that F. hepatica-induced TGF-beta plays a critical role in bystander suppression of autoantigen-specific Th1 and Th17 responses that mediate autoimmune diseases.
Subject(s)
Autoimmunity/immunology , Fascioliasis/immunology , Interleukin-17/immunology , Th1 Cells/immunology , Transforming Growth Factor beta/physiology , Animals , Autoantigens , Fasciola hepatica/immunology , Interleukin-10/biosynthesis , Mice , Peritoneum/immunology , Peritoneum/parasitology , Peritoneum/pathology , Th1 Cells/parasitology , Th2 Cells/immunology , Th2 Cells/parasitology , Transforming Growth Factor beta/biosynthesisABSTRACT
Gnathostoma turgidum is a nematode that parasitizes the stomach of opossums, Didelphis virginiana. Despite its wide distribution in the Americas, its natural life cycle is poorly understood. Recently, we found an endemic area for G. turgidum infection in Sinaloa, Mexico (Diaz-Camacho et al., 2009). Based on sporadic surveys for several years, the prevalence was apparently high in summer and extremely low in winter. To confirm that this is really a seasonal variance, we conducted a longitudinal survey on G. turgidum infection in opossums from November 2007 to November 2008. The results showed amazing seasonal changes in the prevalence, with synchronized migration and maturation of worms in opossums. Between February and March, many juvenile worms, with occasional AL3, were found in the liver, but no worms were found in the stomach. Mature adult worms began to appear in the stomach around April and rapidly increased in number toward July, when all worms resided in the stomach. Then, the worms disappeared almost completely by November. These results suggest that G. turgidum is an annual parasite of the opossum, D. virginiana, in Mexico.
Subject(s)
Didelphis/parasitology , Gnathostoma/physiology , Seasons , Spirurida Infections/veterinary , Animals , Diaphragm/parasitology , Female , Gnathostoma/anatomy & histology , Intestines/parasitology , Liver/parasitology , Male , Mexico/epidemiology , Peritoneum/parasitology , Prevalence , Spirurida Infections/epidemiology , Spirurida Infections/parasitology , Stomach/parasitologyABSTRACT
Plasmacytoid dendritic cells (pDCs) play a key role in the innate immune response to viral infection, due largely to their ability to produce large quantities of type I IFNs. These cells are also notable for their ability to differentiate into conventional dendritic cells after appropriate stimulation. Here, we show that a splenic population of murine CD11c(+) cells expressing pDC markers Gr-1, B220, and PDCA-1 is preferentially parasitized after infection with the virulent RH strain of Toxoplasma gondii. Although these markers are closely associated with pDCs, the population we identified was unusual because the cells express CD11b and higher than expected levels of CD11c. By adoptive transfer of CD45.1-positive cells into CD45.2 congenic mice, we show that CD11c(+)Gr-1(+) cells migrate from the peritoneal cavity to the spleen. During infection, these cells accumulate in the marginal zone region. Recruitment of infected CD11c(+)Gr-1(+) cells to the spleen is partially dependent upon signaling through chemokine receptor CCR2. Intracellular cytokine staining demonstrates that infected, but not noninfected, splenic CD11c(+)Gr-1(+) dendritic cells are suppressed in their ability to respond to ex vivo TLR stimulation. We hypothesize that Toxoplasma exploits pDCs as Trojan horses, targeting them for early infection, suppressing their cytokine effector function, and using them for dissemination within the host.
Subject(s)
Antigens, Surface/biosynthesis , Dendritic Cells/immunology , Dendritic Cells/parasitology , Toxoplasmosis/immunology , Toxoplasmosis/parasitology , Animals , Antigens, Surface/genetics , Biomarkers/metabolism , CD11c Antigen/biosynthesis , CD11c Antigen/genetics , Cells, Cultured , Dendritic Cells/metabolism , Female , Immunophenotyping , Interleukin-12/antagonists & inhibitors , Interleukin-12/biosynthesis , Mice , Mice, Congenic , Mice, Inbred C57BL , Mice, Knockout , Peritoneum/immunology , Peritoneum/parasitology , Peritoneum/pathology , Receptors, Chemokine/biosynthesis , Receptors, Chemokine/genetics , Toxoplasma/genetics , Toxoplasma/growth & development , Toxoplasma/immunology , Toxoplasmosis/metabolismABSTRACT
Infection caused by Echinococcus multilocularis is a rare helminthiasis, human cases have not been diagnosed in Hungary until now. The endemic region is Central Europe; the occurrence of this infection has been reported from most of the neighbouring countries; however, E. multilocularis has been found in the red fox population in Hungary. Summarizing the recent knowledge concerning epidemiological, clinical patterns and therapeutic options, the authors describe the first Hungarian case of alveolar echinococcosis. In the presence of appropriate clinical findings, the possibility of this rare infection has to be considered in the differential diagnosis of infiltrative hepatic lesions.