Synergistic effect of silicon availability and salinity on metal adsorption in a common estuarine diatom.

Increasing nitrogen and phosphorus discharge and decreasing sediment input have made silicon (Si) a limiting element for diatoms in estuaries. Disturbances in nutrient structure and salinity fluctuation can greatly affect metal uptake by estuarine diatoms. However, the combined effects of Si and salinity on metal accumulation in these diatoms have not been evaluated. In this study, we aimed to investigate how salinity and Si availability combine to influence the adsorption of metals by a widely distributed diatom Phaeodactylum tricornutum. Our data indicate that replete Si and low salinity in seawater can enhance cadmium and copper adsorption onto the diatom surface. At the single-cell level, surface potential was a dominant factor determining metal adsorption, while surface roughness also contributed to the higher metal loading capacity at lower salinities. Using a combination of non-invasive micro-test technology, atomic force microscopy, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy, we demonstrate that the diversity and abundance of the functional groups embedded in diatom cell walls vary with salinity and Si supply. This results in a change in the cell surface potential and transient metal influx. Our study provides novel mechanisms to explain the highly variable metal adsorption capacity of a model estuarine diatom.

Cotransport of nanoplastics with nZnO in saturated porous media: From brackish water to seawater.

The ocean serves as a repository for various types of artificial nanoparticles. Nanoplastics (NPs) and nano zinc oxide (nZnO), which are frequently employed in personal care products and food packaging materials, are likely simultaneously released and eventually into the ocean with surface runoff. Therefore, their mutual influence and shared destiny in marine environment cannot be ignored. This study examined how nanomaterials interacted and transported through sea sand in various salinity conditions. Results showed that NPs remained dispersed in brine, while nZnO formed homoaggregates. In seawater of 35 practical salinity units (PSU), nZnO formed heteroaggregates with NPs, inhibiting NPs mobility and decreasing the recovered mass percentage (Meff) from 24.52% to 12.65%. In 3.5 PSU brackish water, nZnO did not significantly aggregate with NPs, and thus barely affected their mobility. However, NPs greatly enhanced nZnO transport with Meff increasing from 14.20% to 25.08%, attributed to the carrier effect of higher mobility NPs. Cotransport from brackish water to seawater was simulated in salinity change experiments and revealed a critical salinity threshold of 10.4 PSU, below which the mobility of NPs was not affected by coexisting nZnO and above which nZnO strongly inhibited NP transport. This study highlights the importance of considering the mutual influence and shared destiny of artificial nanoparticles in the marine environment and how their interaction and cotransport are dependent on changes in seawater salinity.

Chemical composition and egg production capacity throughout bloom development of ctenophore Mnemiopsis leidyi in the northern Adriatic Sea.

High abundances of gelatinous zooplankton (GZ) can significantly impact marine ecosystem by acting as both sink and source of organic matter (OM) and nutrients. The decay of GZ bloom can introduce significant amount of OM to the ocean interior, with its variability influenced by GZ life traits and environmental factors, impacting microbial communities vital to marine biogeochemical cycles. The invasive ctenophores Mnemiopsis leidyi has formed massive blooms in the northern Adriatic Sea since 2016. However, the variability in the chemical composition and egg production of blooming populations, as well as the role of environmental factors in governing this variability, remains largely unknown. Our analysis of biometry, chemical composition, and fecundity of M. leidyi sampled in the Gulf of Trieste in 2021 revealed stable carbon and nitrogen content throughout bloom development, with no significant correlation with seawater temperature, salinity, oxygen, and chlorophyll a concentration. Although the studied population exhibited homogeneity in terms of biometry and chemical composition, the number of produced eggs varied substantially, showing no clear correlation with environmental variables and being somewhat lower than previously reported for the study area and other Mediterranean areas. We observed a positive correlation between the wet weight of individuals and the percentage of hatched eggs, as well as a significant positive correlation between the percentage of hatched eggs and ambient seawater temperature. Additionally, we noted that the speed of hatching decreased with decreasing seawater temperature in autumn, corresponding to the end of M. leidyi bloom.

Nitrogen Fixation and Microbial Communities Associated with Decomposing Seagrass Leaves in Temperate Coastal Waters.

Seagrass meadows play pivotal roles in coastal biochemical cycles, with nitrogen fixation being a well-established process associated with living seagrass. Here, we tested the hypothesis that nitrogen fixation is also associated with seagrass debris in Danish coastal waters. We conducted a 52-day in situ experiment to investigate nitrogen fixation (proxied by acetylene reduction) and dynamics of the microbial community (16S rRNA gene amplicon sequencing) and the nitrogen fixing community (nifH DNA/RNA amplicon sequencing) associated with decomposing Zostera marina leaves. The leaves harboured distinct microbial communities, including distinct nitrogen fixers, relative to the surrounding seawater and sediment throughout the experiment. Nitrogen fixation rates were measurable on most days, but highest on days 3 (dark, 334.8 nmol N g-1 dw h-1) and 15 (light, 194.6 nmol N g-1 dw h-1). Nitrogen fixation rates were not correlated with the concentration of inorganic nutrients in the surrounding seawater or with carbon:nitrogen ratios in the leaves. The composition of nitrogen fixers shifted from cyanobacterial Sphaerospermopsis to heterotrophic genera like Desulfopila over the decomposition period. On the days with highest fixation, nifH RNA gene transcripts were mainly accounted for by cyanobacteria, in particular by Sphaerospermopsis and an unknown taxon (order Nostocales), alongside Proteobacteria. Our study shows that seagrass debris in temperate coastal waters harbours substantial nitrogen fixation carried out by cyanobacteria and heterotrophic bacteria that are distinct relative to the surrounding seawater and sediments. This suggests that seagrass debris constitutes a selective environment where degradation is affected by the import of nitrogen via nitrogen fixation.

The ocean losing its breath under the heatwaves.

The world's oceans are under threat from the prevalence of heatwaves caused by climate change. Despite this, there is a lack of understanding regarding their impact on seawater oxygen levels - a crucial element in sustaining biological survival. Here, we find that heatwaves can trigger low-oxygen extreme events, thereby amplifying the signal of deoxygenation. By utilizing in situ observations and state-of-the-art climate model simulations, we provide a global assessment of the relationship between the two types of extreme events in the surface ocean (0-10 m). Our results show compelling evidence of a remarkable surge in the co-occurrence of marine heatwaves and low-oxygen extreme events. Hotspots of these concurrent stressors are identified in the study, indicating that this intensification is more pronounced in high-biomass regions than in those with relatively low biomass. The rise in the compound events is primarily attributable to long-term warming primarily induced by anthropogenic forcing, in tandem with natural internal variability modulating their spatial distribution. Our findings suggest the ocean is losing its breath under the influence of heatwaves, potentially experiencing more severe damage than previously anticipated.

Insights into the physiology, biochemistry and ecological significance of the red seaweed Tricleocarpa fragilis in the Andaman Sea.

The present study focused on the physiological and biochemical aspects of Tricleocarpa fragilis, red seaweed belonging to the phylum Rhodophyta, along the South Andaman coast, with particular attention given to its symbiotic relationships with associated flora and fauna. The physicochemical parameters of the seawater at the sampling station, such as its temperature, pH, and salinity, were meticulously analyzed to determine the optimal harvesting period for T. fragilis. Seaweeds attach to rocks, dead corals, and shells in shallow areas exposed to moderate wave action because of its habitat preferences. Temporal variations in biomass production were estimated, revealing the highest peak in March, which was correlated with optimal seawater conditions, including a temperature of 34 ± 1.1 °C, a pH of 8 ± 0.1, and a salinity of 32 ± 0.8 psu. GC‒MS analysis revealed n-hexadecanoic acid as the dominant compound among the 36 peaks, with major bioactive compounds identified as fatty acids, diterpenes, phenolic compounds, and hydrocarbons. This research not only enhances our understanding of ecological dynamics but also provides valuable insights into the intricate biochemical processes of T. fragilis. The established antimicrobial potential and characterization of bioactive compounds from T. fragilis lay a foundation for possible applications in the pharmaceutical industry and other industries.

From Sea Water to Salt Crystals: An Onsite Investigation of Microplastics in a Conventional Sea Salt Farming System.

This study investigated microplastic (MP) contamination in conventional sea salt farming systems. Various crude sea salt samples (n = 22) that were traditionally produced were collected from salt farms and local vendors. Salt water (n = 15), macroalgae (n = 6), and clay of pond floors (n = 6) were collected from ponds subjected to different production (stabilization, evaporation, and concentration and crystallization concentration) processes. All samples were analyzed for MP abundance and characteristics. The potential sources of MP contamination in the salt were also investigated. The mean abundance of MPs in the salt water and clay of pond floor increased progressively throughout the production process and reached its highest level in the concentration and crystallization ponds (7400 MP particles/m3 in salt water and 19,336 MP particles/m2 in the clay of the pond floor). A maximum of 26,500 MP particles/kg of macroalgal material indicated the potential sink of MPs on the surface of the algae. Approximately 34-2377 MP particles/kg salt were found in the crude sea salt samples. However, the mean abundance (378 MP particles/kg of salt) indicated nonsignificant impacts of different harvesting processes on MP contamination. Most MP size distributions, shapes and polymer types in the salts were similar to those found in the salt water, macroalgae and clay of the pond floor. Approximately 99% of the MPs were fragments that were suspected to be decomposed from larger plastic debris and plastic machinery and tools used at the salt farm. Similar patterns of polymer distribution, in which PP > PE > PET > PS, were found for all samples studied.

Phaeocystis globosa and diatom blooms promote distinct bacterial communities and associations in a coastal ecosystem.

Phytoplankton and bacteria form the foundation of marine food webs. While most studies on phytoplankton bloom influence on bacteria dynamics focus on diatom-dominated blooms due to their global ecological significance, it is unclear if similar patterns extend to other species that compete with diatoms like Phaeocystis spp. This study aimed to contribute to the understanding of associations between phytoplankton and bacteria in a temperate ecosystem. For this, we studied the dynamics of phytoplankton and bacteria, combining 16S metabarcoding, microscopy, and flow cytometry over 4 years (282 samples). Phytoplankton and bacterial communities were studied throughout the year, particularly during contrasting phytoplankton blooms dominated by the Haptophyte Phaeocystis globosa or diatoms. We applied extended local similarity analysis (eLSA) to construct networks during blooming and non-blooming periods. Overall, the importance of seasonal and species-specific interactions between phytoplankton and bacteria is highlighted. In winter, mixed diatom communities were interconnected with bacteria, indicating a synergistic degradation of diverse phytoplankton-derived substrates. In spring, despite the intensity variations of P. globosa blooms, the composition of bacterial communities remained consistent over several years, suggesting establishing a stable-state environment for bacterial communities. Specific associations between monospecific diatom blooms and bacteria were evidenced in summer.

Carbonate chimneys at the highly productive point Dume methane seep: Fine-scale mineralogical, geochemical, and microbiological heterogeneity reflects dynamic and long-lived methane-metabolizing habitats.

Methane is a potent greenhouse gas that enters the marine system in large quantities at seafloor methane seeps. At a newly discovered seep site off the coast of Point Dume, CA, ~ meter-scale carbonate chimneys host microbial communities that exhibit the highest methane-oxidizing potential recorded to date. Here, we provide a detailed assessment of chimney geobiology through correlative mineralogical, geochemical, and microbiological studies of seven chimney samples in order to clarify the longevity and heterogeneity of these highly productive systems. U-Th dating indicated that a methane-driven carbonate precipitating system at Point Dume has existed for ~20 Kyr, while millimeter-scale variations in carbon and calcium isotopic values, elemental abundances, and carbonate polymorphs revealed changes in carbon source, precipitation rates, and diagenetic processes throughout the chimneys' lifespan. Microbial community analyses revealed diverse modern communities with prominent anaerobic methanotrophs, sulfate-reducing bacteria, and Anaerolineaceae; communities were more similar within a given chimney wall transect than in similar horizons of distinct structures. The chimneys represent long-lived repositories of methane-oxidizing communities and provide a window into how carbon can be transformed, sequestered, and altered over millennia at the Point Dume methane seep.

Enhanced nutrient supply promotes mutualistic interactions between cyanobacteria and bacteria in oligotrophic ocean.

Cyanobacteria can form complex interactions with heterotrophic microorganisms, but this relationship is susceptible to nutrient concentrations. Disentangling the cyanobacteria-bacteria interactions in relation to nutrient supply is essential to understanding their roles in geochemical cycles under global change. We hypothesize that enhanced nutrient supply in oligotrophic oceans can promote interactions among cyanobacteria and bacteria. Therefore, we investigated the planktonic bacteria and their interactions with cyanobacteria in relation to elevated nutrients caused by enhanced upwelling around a shallow and a deep seamount in the tropical western Pacific Ocean. We found obviously higher complexity of network occurred with significantly more cyanobacteria in the deep chlorophyll maximum layer of the shallow seamount when compared with that of the deep seamount. Cyanobacteria can shape bacterial interaction and community evenness in response to relatively high nutrient concentrations. The effects of the nutrients on cyanobacteria-related networks were further estimated based on the Tara Oceans data. Statistical analyses further showed a facilitative effect of nitrate concentrations on cyanobacteria-bacteria mutualistic interactions in the global oligotrophic ocean. By analysing the Tara Ocean macrogenomic data, we detected functional genes related to cyanobacteria-bacteria interactions in all samples, indicating the existence of a mutualistic relationship. Our results reveal cyanobacteria-bacteria interaction in response to nutrient elevation in oligotrophic ocean and highlight the potentially negative effects of global change on the bacterial community from the view of the bio-interaction.

Assessing Chlorophyll-a Variations in Caspian Sea during the COVID-19 Pandemic.

The COVID-19 pandemic's disruptions to human activities prompted serious environmental changes. Here, we assessed the variations in coastal water quality along the Caspian Sea, with a focus on the Iranian coastline, during the lockdown. Utilizing Chlorophyll-a data from MODIS-AQUA satellite from 2015 to 2023 and Singular Spectrum Analysis for temporal trends, we found a 22% Chlorophyll-a concentration decrease along the coast, from 3.2 to 2.5 mg/m³. Additionally, using a deep learning algorithm known as Long Short-Term Memory Networks, we found that, in the absence of lockdown, the Chlorophyll-a concentration would have been 20% higher during the 2020-2023 period. Furthermore, our spatial analysis revealed that 98% of areas experienced about 18% Chlorophyll-a decline. The identified improvement in coastal water quality presents significant opportunities for policymakers to enact regulations and make local administrative decisions aimed at curbing coastal water pollution, particularly in areas experiencing considerable anthropogenic stress.

Effects of marine eutrophication environment on microbial corrosion: A review.

Metal materials undergo severe corrosion in eutrophic environments. The effect of DO decay stimulated by high concentrations of nitrogen and phosphorus pollutants on microorganisms leads to the coupling of electrochemical and microbial corrosion processes. However, there are few studies on microbial corrosion mechanisms in eutrophic environments. This article discusses the corrosive factors of marine eutrophication, summarizes the impact of marine eutrophication on microbial corrosion and the potential mechanisms, including aerobic biofilm corrosion, aerobic & anaerobic mixed biofilm corrosion, and anaerobic microbial electron transfer corrosion, and expounds on the research methods for microbial corrosion of materials serving in estuarine areas prone to pollution. Microbial prevention and control, such as nutrient restriction and microbial interspecies competition, are of research value in the field of green protection. Microbial corrosion mechanisms studies in marine eutrophication environments are significant for environment monitor development, water intake and algae control technologies, and corrosion protection in polluted environments.

Unveiling a new oceanic anoxic event at the Norian/Rhaetian boundary (Late Triassic).

The latest Triassic was characterised by protracted biotic extinctions concluding in the End-Triassic Extinction (~ 200 Ma) and a global carbon cycle perturbation. The onset of declining diversity is closely related to reducing conditions that spread globally from upper Sevatian (uppermost Norian) to across the Norian-Rhaetian boundary, likely triggered by unusually high volcanic activity. We correlate significant organic carbon cycle perturbations to an increase of CO2 in the ocean-atmosphere system, likely outgassed by the Angayucham igneous province, the onset of which is indicated by the initiation of a rapid decline in 87Sr/86Sr and 188Os/187Os seawater values. A possible causal mechanism involves elevated CO2 levels causing global warming and accelerating chemical weathering, which increased nutrient discharge to the oceans and greatly increased biological productivity. Higher export production and oxidation of organic matter led to a global O2 decrease in marine water across the Norian/Rhaetian boundary (NRB). Biotic consequences of dysoxia/anoxia include worldwide extinctions in some fossil groups, such as bivalves, ammonoids, conodonts, radiolarians.

Future changes in coastal upwelling and biological production in eastern boundary upwelling systems.

Upwelling along oceanic eastern boundaries has attracted significant attention due to its profound effects on ocean productivity and associated biological and socioeconomic implications. However, uncertainty persists regarding the evolution of coastal upwelling with climate change, particularly its impact on future biological production. Here, using a series of state-of-the-art climate models, we identify a significant seasonal advancement and prolonged duration of upwelling in major upwelling systems. Nevertheless, the upwelling intensity (total volume of upwelled water) exhibits complex changes in the future. In the North Pacific, the upwelling is expected to attenuate, albeit with a minor magnitude. Conversely, in other basins, coastal upwelling diminishes significantly in equatorward regions but displays a slight decline or even an enhancement at higher latitudes. The climate simulations also reveal a robust connection between changes in upwelling intensity and net primary production, highlighting the crucial impact of future coastal upwelling alterations on marine ecosystems.

Distribution, Source, and Contamination of Heavy Metals in Coastal Sediments of Jeddah, Red Sea, Saudi Arabia.

The study investigates heavy metal (HM) contamination in coastal sediments of Jeddah along Red Sea coast, analyzing spatial distribution and sources. 24 samples underwent (ICP-AES) for Fe, Al, Mn, Ni, Pb, Zn, Cu, Cr, Co, Sr, V, and As. HM averages followed Fe ˃ Al ˃ Sr ˃ Mn ˃ Zn ˃ V ˃ Cu ˃ Ni ˃ Cr ˃ As ˃ Co ˃ Pb. Contamination indices revealed severe Sr enrichment, minor As and Co enrichment, and no enrichment for other HMs. Sediment quality guidelines suggest Ni, Cu, Zn, and As risks to benthic communities at some sites, while Cr and Pb pose minimal risk. Multivariate analysis indicates natural sources for Fe, Al, Mn, Ni, Zn, Cu, Cr, Co, and V, and anthropogenic sources for Sr, As, and Pb, linked to agriculture, industry, and urbanization. Increased Sr values may stem from seawater acidification impacting calcitic corals and molluscs.

A novel indicator of anthropogenic influence on the fluctuability and stability of phytoplankton community composition.

Marine community composition is expected to be relatively stable in a natural environment over time but shift under increasing anthropogenic disturbances. In coastal waters, diatoms and dinoflagellates are two dominant phytoplankton functional groups. In this study, we developed an areal phytoplankton community composition index (APCI) that is based on the area of a scatter plot of dinoflagellate abundance (y-axis) vs diatom abundance (x-axis) using a time window of 1 year, 2 years or 3 years data. An APCI allows an ecological interpretation: it represents the fluctuability of a community composition within a time window and a temporal change between two neighbouring APCIs in a time series represents the stability of the composition. We used a 28-yr time series of monthly data on diatom and dinoflagellate abundance at four stations in Tolo Harbour and Channel (Tolo), Hong Kong to test the hypothesis that temporal changes in APCIs indicate environmental disturbances and to examine the applicability of APCI to indicate changes in nutrient conditions. We calculated the area (APCI) of a scatter plot of monthly data for 1-year, 2-year and 3-year windows, referred to as APCI-1y, -2y and -3y, respectively. The results show that, the fluctuability, is larger in APCI-3y than in APCI-1y, while the stability is stronger as temporal changes between neighbouring APCI-3y are smaller than between APCI-1ys. Temporal trends of APCIs are significantly correlated with those of dissolved inorganic nitrogen and phosphate concentration, which have declined after the implementation of a sewage diversion management plan in 1998. Hence, the APCI method is likely a robust indicator to assess a response of the phytoplankton community composition in a water body to environmental disturbances.

Dynamics of Noctiluca scintillans blooms: A 20-year study in Jangmok Bay, Korea.

This 20-year study (2001-2020) conducted in Jangmok Bay, Korea, assessed the intricate relationships between environmental factors and Noctiluca scintillans blooms. Granger causality tests and PCA analysis were used to assess the impact of sea surface temperature (SST), salinity, dissolved oxygen (DO) concentration, wind patterns, rainfall, and chlorophyll-a (Chl-a) concentration on bloom dynamics. The results revealed significant, albeit delayed, influences of these variables on bloom occurrence, with SST exhibiting a notable 2-month lag and salinity a 1-month lag in their impact. Additionally, the analysis highlighted the significant roles of phosphate, ammonium, and silicate, which influenced N. scintillans blooms with lags of 1 to 3 months. The PCA demonstrates how SST and wind speed during spring and summer, along with wind direction and salinity in winter, significantly impact N. scintillans blooms. We noted not only an increase in large-scale N. scintillans blooms but also a cyclical pattern of occurrence every 3 years. These findings underscore the synergistic effects of environmental factors, highlighting the complex interplay between SST, salinity, DO concentration, and weather conditions to influence bloom patterns. This research enhances our understanding of harmful algal blooms (HABs), emphasizing the importance of a comprehensive approach that considers multiple interconnected environmental variables for predicting and managing N. scintillans blooms.

Assessment of the effects of dredging on metal levels in port waters using DGT passive samplers and spot sampling.

Aiming at assessing the effect of dredging activities on the levels of metals in Bilbao Port (northern Spain), dissolved and labile metal concentrations in the water were concurrently measured, before, during, and after dredging activities by spot sampling and Diffusive Gradients in Thin-films (DGTs) passive samplers, respectively. Most of the dissolved metal results were below the quantification limits (Cd, <0.06-0.26 µg/L; Co, <5 µg/L; Cu, <5-15 µg/L; Fe, <10-48 µg/L; Mn, <10-22 µg/L; Ni, <2.6-7 µg/L; Pb, <0.39-0.8 µg/L; Zn, <9-24 µg/L). In contrast, DGT results for all sampling times and stations were obtained (Cd, 0.02-0.12 µg/L; Co, 0.08-0.15 µg/L; Cu, 0.5-2.8 µg/L; Fe, 1.0-3.6 µg/L; Mn, 4.7-23.5 µg/L; Ni, 0.5-0.9 µg/L; Pb, 0.15-0.28 µg/L; Zn, 2.6-7.2 µg/L), enabling to determine those metals affected by dredging. Only labile-Pb concentration surpassed momentarily the DGT-Environmental Quality Standard, enabling to rule out biological effects on biota. DGTs are a promising technique for facilitating decision-making during dredging operations.

Dispersion analysis of the 2017 Persian Gulf oil spill based on remote sensing data and numerical modelling.

Oil spills, detected by SAR sensors as dark areas, are highly effective marine pollutants that affect the ocean surface. These spills change the water surface tension, attenuating capillary gravitational waves and causing specular reflections. We conducted a case study in the Persian Gulf (Arabian Sea to the Strait of Hormuz), where approximately 163,900 gal of crude oil spilled in March 2017. Our study examined the relationship between oil weathering processes and extracted backscatter values using zonal slices projected over SAR-detected oil spills. Internal backscatter values ranged from -22.5 to -23.5, indicating an oil chemical binding and minimal interaction with seawater. MEDSLIK-II simulations indicated increased oil solubilization and radar attenuation rates with wind, facilitating coastal dispersion. Higher backscatter at the spill edges compared to the core reflected different stages of oil weathering. These results highlight the complex dynamics of oil spills and their environmental impact on marine ecosystems.