[Analysis on formula of Mongolian medicine for prevention of COVID-19].

The epidemic situation of coronavirus disease 2019(COVID-19) is developing rapidly in the world, and the influence is serious. In this study, the prescription of Mongolian medicine to prevent new type of COVID-19 was investigated. Based on the second edition and the third edition of COVID-19 Mongolian Medicine Prevention and Treatment Guidance Program issued by the Inner Mongolia Autonomous Region Health Commission, using Excel 2007, SPSS Modeler 18, SPSS Statistics 25, Cytoscape 3.7.1 statistical software as a tool, the association rules analysis and cluster analysis of Mongolian medicine included in the standard were carried out. Among the 45 prophylactic prescriptions included in the standard, a total of 34 high-frequency drugs using frequency ≥5 were used, of which Carthami Flos(21 times, 4.46%), Chebulae Fructus(20 times, 4.26%), Moschus(13 times, 2.77%), Myristicae Semen(12 times, 2.55%), Santali Albi Lignum(12 times, 2.55%), and Bovis Calculus(12 times, 2.55%) were the most common. The main drugs for the prevention of COVID-19 were Liang(13 times, 38.23%), Wen(9 times, 26.47%), the flavor was Ku(20 times, 34.48%), Xin(13 times, 22.41%), Gan(11 times, 18.97%), the most used drugs treating hot evil(99 times, 32.46%), treatment of "Heyi" drugs(51 times, 16.72%), treatment of "Badagan" drugs(40 times, 13.11%), treatment of "sticky" drugs(37 times, 12.13%), and a cough, eliminating phlegm and antiasthmatic(31 times, 10.16%), the association rule analysis found that the highest association intensity of the drug pair combination of 11. Clustering analysis using the cluster analysis of inter-group join method found a total of 8 categories. In this study, 45 prescriptions of Mongolian medicine for the prevention of COVID-19 were collec-ted and further analyzed, hoping to provide new ideas for clinical diagnosis and treatment.

[Effects of electric mildly-warmed needle of inner mongolian medicine on liver MDA and GSH content, GSH-Px and SOD activity in fatigue rats].

OBJECTIVE: To investigate the effect of electric mildly-warmed needle of inner Mongolian medicine on changes of free radical metabolism in the liver tissue of rats with fatigue. METHODS: A total of 33 male SD rats were evenly randomized into control, model and electro-mildly-warmed needle (EMWN) groups. Fatigue model was established by forcing the rat to swim in a water pool till exhaustion, once daily, continuously for 21 days. "Dinghui" (central spot of the bregmatic bone) and "Xinxue" (the center of the depression beneath the 7th thoracic vertebra) were punctured with silver needle which was warmed electrically by using a MLY-I Electrical Needle-warming Apparatus, once every 3 days. 7 sessions altogether. After decapitation, the rat's liver was taken, homogenated and centrifuged for detecting malondialdehyde (MDA) content with thio-barbituric acid, reduced glutathione (GSH) contents, glutathione peroxidase (GSH-Px) activity with chromatometry and superoxide dismutase (SOD) activity with xanthine oxidase method respectively. RESULTS: Compared with control group, liver GSH content, GSH-Px and SOD activity in model group lowered significantly (P < 0.05, 0.01), while in comparison with model group, these 3 indexes of EMWN group increased considerably (P < 0.05, 0.01). No significant differences were found among 3 groups in MDA levels (P > 0.05). The results showed that the effect of mildly-warmed needle treatment could resist fatigue induced decline of activity of antioxidant enzymes. CONCLUSION: Electric mildly-warmed needle therapy of inner Mongolian medicine can raise the activity of antioxidant enzymes in liver tissue of fatigue rats, which may underlie the action of mildly-warmed needle in improving fatigue.