RESUMO
Snake venom metalloproteinases (SVMPs) are enzymatic proteins present in large amounts in snake venoms presenting proteolytic, hemorrhagic, and coagulant activities. BjussuMP-II, a class P-I SVMP, isolated from the Bothrops jararacussu snake venom does not have relevant hemorrhagic activity but presents fibrinolytic, fibrinogenolytic, antiplatelet, gelatinolytic, and collagenolytic action. This study aimed to verify the action of BjussuMP-II on human neutrophil functionality focusing on the lipid bodies formation and hydrogen peroxide production, the release of dsDNA through colorimetric and microscopic assays, and cytokines by immunoenzymatic assays. Results showed that BjussuMP-II at concentrations of 1.5 up to 50 µg/mL for 24 h is not toxic to human neutrophils using an MTT assay. Under non-cytotoxic concentrations, BjussuMP-II can induce an increase in the formation of lipid bodies, production of hydrogen peroxide and cytokines [tumor necrosis factor-α (TNF-α), interleukin-1ß (IL-1ß), interleukin-6 (IL-6) and interleukin-8 (IL-8)] liberation and, the release of dsDNA to form NETs. Taken together, the data obtained show for the first time that BjussuMP-II has a pro-inflammatory action and activates human neutrophils that can contribute to local damage observed in snakebite victims.
Assuntos
Bothrops , Venenos de Crotalídeos , Animais , Humanos , Venenos de Crotalídeos/metabolismo , Neutrófilos , Bothrops/genética , Peróxido de Hidrogênio/metabolismo , Metaloproteases/metabolismo , Citocinas/metabolismo , Interleucina-6RESUMO
SUMMARY The present study is a review of data available in Brazil on bacterial diseases transmitted through the consumption of seafood and related products. Data are presented regarding outbreaks and cases of disease and laboratory findings associated with pathogens in seafood and related products, and methods for prevention and control are described. .
RESUMO Esta revisão apresenta dados qualitativos e quantitativos sobre doenças bacterianas e achados laboratoriais associados ao consumo de pescado e derivados no Brasil de 1983 a 2011. Os resultados mostram uma séria lacuna de dados epidemiológicos relacionados a surtos causados por pescado. Os poucos dados disponíveis indicam que em contraste com outros surtos alimentares transmitidos por carne, aves, laticínios e vegetais, as bactérias patogênicas teriam um menor destaque na transmissão destas doenças pelo consumo de pescado e derivados. Vibrio parahaemolyticus parece ser a causa mais frequente das doenças causadas pelo consumo de pescado e derivados, bem como a bactéria patogênica mais comumente presente nesses produtos nas investigações laboratoriais. .
