Beyond probiotics, uses of their next-generation for poultry and humans: A review.

The production of healthy food is one of the basic requirements and challenges. Research efforts have been introduced in the human's food industry to reduce the microbial resistance and use safe and healthy alternatives with a high durability. However, the conducted work about these issues in the field of livestock animal production have been started since 2015. Inappropriate and extensive use of antibiotics has resulted in the increase of antimicrobial resistance, presence of drug residues in tissues, and destruction of the gut microbiome. Therefore, discovering and developing antibiotic substitutes were urgent demands. Probiotic compounds containing living micro-organisms are important antibiotic alternative that have been beneficially and extensively used in humans, animals, and poultry. However, some probiotics show some obstacles during production and applications. Accordingly, this review article proposes a comprehensive description of the next-generation of probiotics including postbiotics, proteobiotics, psychobiotics, immunobiotics and paraprobiotics and their effects on poultry production and human's therapy. These compounds proved great efficiency in terms of restoring gut health, improving performance and general health conditions, modulating the immune response and reducing the pathogenic micro-organisms. However, more future research work should be carried out regarding this issue.

Potential Effects of Garlic (Allium sativum L.) on the Performance, Immunity, Gut Health, Anti-Oxidant Status, Blood Parameters, and Intestinal Microbiota of Poultry: An Updated Comprehensive Review.

The use of antibiotics as growth promoters or for the prevention of some poultry diseases has faced global concern and serious criticism. Their addition to poultry feed has shown hazardous effects, including the development of antimicrobial resistance and a potentially harmful effect on human health. To eliminate these threats, there is increasing interest in natural alternatives. Plant derivatives such as garlic (Allium sativum L.) and its derivatives are presently extensively used in the poultry production system. The dietary supplementation of broilers and layers with garlic induced improvement in the production parameters, carcass quality, and intestinal integrity. The modulation of the immune response against some important viral diseases has resulted from the supplementation of poultry with garlic. Moreover, garlic has been shown to modulate gut health through antibacterial and antiparasitic activities. Treatment with garlic can also mitigate oxidative stress and reduce free-radical production. The reduction of cholesterol levels and improvement of some liver and blood parameters were also reported following the dietary inoculation of garlic. This review was designed to investigate the influence of garlic as a dietary additive on the performance, immunity, gut health, anti-oxidant status, blood parameters, and intestinal microbiota of poultry.

Resistance profiles, virulence and antimicrobial resistance genes of XDR S. Enteritidis and S. Typhimurium.

Avian salmonellosis is concomitant with high financial crises in the poultry industry as well as food-borne illness in man. The present study is designed to investigate the emergence of Salmonella Enteritidis and Salmonella Typhimurium in diseased broilers, resistance profiles, and monitoring virulence and antibiotic resistance genes. Consequently, 450 samples (cloacal swabs, liver, and spleen) were collected from 150 diseased birds from different farms in Giza Governorate, Egypt. Subsequently, the bacteriological examination was done. Afterward, the obtained Salmonella isolates were tested for serogrouping, antibiogram, PCR monitoring of virulence (invA, stn, hilA, and pefA), and antimicrobial resistance genes (blaTEM, blaCTX-M, blaNDM, ermA, sul1, tetA, and aadA1). The total prevalence of Salmonella in the examined diseased broilers was 9.3%, and the highest prevalence was noticed in cloacal swabs. Among the recovered Salmonella isolates (n = 35), 20 serovars were recognized as S. Enteritidis and 15 serovars were identified as S. Typhimurium. Almost 60% of the retrieved S. Enteritidis serovars were extensively drug-resistant (XDR) to seven antimicrobial classes and inherited sul1, blaTEM, tetA, blaCTX-M, ereA, and aadA1 genes. Likewise, 25% of the recovered S. Enteritidis serovars were multidrug-resistant (MDR) to six classes and have sul1, blaTEM, tetA, blaCTX-M, and ereA resistance genes. Also, 66.7% of the retrieved S. Typhimurium serovars were XDR to seven classes and have sul1, blaTEM, tetA, blaCTX-M, ereA, and aadA1 genes. Succinctly, this report underlined the reemergence of XDR S. Typhimurium and S. Enteritidis in broiler chickens. Meropenem and norfloxacin exposed a hopeful antimicrobial activity toward the re-emerging XDR S. Typhimurium and S. Enteritidis in broilers. Moreover, the recurrence of these XDR Salmonella strains poses a potential public health threat.

Gallibacterium anatis infection in poultry: a comprehensive review.

Gallibacterium anatis (G. anatis), a member of the Pasteurellaceae family, normally inhabits the upper respiratory and lower genital tracts of poultry. However, under certain circumstances of immunosuppression, co-infection (especially with Escherichia coli or Mycoplasma), or various stressors, G. anatis caused respiratory, reproductive, and systemic diseases. Infection with G. anatis has emerged in different countries worldwide. The bacterium affects mainly chickens; however, other species of domestic and wild birds may get infected. Horizontal, vertical, and venereal routes of G. anatis infection have been reported. The pathogenicity of G. anatis is principally related to the presence of some essential virulence factors such as Gallibacterium toxin A, fimbriae, haemagglutinin, outer membrane vesicles, capsule, biofilms, and protease. The clinical picture of G. anatis infection is mainly represented as tracheitis, oophoritis, salpingitis, and peritonitis, while other lesions may be noted in cases of concomitant infection. Control of such infection depends mainly on applying biosecurity measures and vaccination. The antimicrobial sensitivity test is necessary for the correct treatment of G. anatis. However, the development of multiple drug resistance is common. This review article sheds light on G. anatis regarding history, susceptibility, dissemination, virulence factors, pathogenesis, clinical picture, diagnosis, and control measures.

A review on aeromoniasis in poultry: A bacterial disease of zoonotic nature.

Aeromonas spp. are widely distributed in surface water, sewage, untreated and chlorinated, drinking water, as well as meats, fish, shellfish, poultry, and their products. A disease caused by Aeromonas spp. is designated as aeromoniasis. It can affect different aquatic animals, mammals, and birds in different geographic regions. Moreover, gastrointestinal and extra-intestinal disease conditions may be provoked in humans as a result of food poising with Aeromonas spp. Some Aeromonas spp. have been identified, however, Aeromonas hydrophila (A. hydrophila), A. caviae, and A. veronii bv sobria may be of public health significance. Aeromonas spp. are members of family Aeromonadaceae and genus Aeromonas. They are Gram-negative rod-shaped, facultative anaerobic, and oxidase and catalase-positive bacteria. The pathogenicity of Aeromonas in different hosts is mediated by several virulence factors such as endotoxins, cytotoxic enterotoxin, cytotoxins, hemolysins, adhesins, and extracellular enzymes such as proteases, amylases, lipases, ADP-ribosyltransferases, and DNases. Most avian species are susceptible to either natural or experimental infections with Aeromonas spp. Infection usually arises through feacal-oral route. Traveler's diarrhea as well as other systemic and local infections are the clinical picture of food poisoning associated with aeromoniasis in humans. Despite Aeromonas spp. being sensitive to various antimicrobials, multiple drug resistance has been commonly reported worldwide. Accordingly, this review highlights aeromoniasis in poultry regarding Aeromonas virulence factors epidemiology, pathogenicity, zoonosis, and antimicrobial resistance.

Betaine: A Potential Nutritional Metabolite in the Poultry Industry.

Poultry industry has been recognized as a fast-developing sector aiming to produce low-cost and high-nutrient foods for human consumption. This review article aimed to focus on the significant roles of dietary betaine supplementation in the poultry industry. In this respect, different effects of betaine on performance and carcass traits, as well as its osmoregulatory, anticoccidial, immune-modulatory, and heat-stress alleviation activities, were discussed. Different concentrations of betaine supplementation can improve the feed conversion ratio, final body weight, breast muscle yield, egg production ratio, and reduce body fat contents in broiler chicken, turkey, duck, geese, and quail diets. Betaine supplemented with methyl groups can eliminate the need to have some methyl-group donors, including choline and methionine, therefore having positive effects on feed conversion ratio in poultry diets. The osmolytic character of betaine can alleviate heat stress and have a positive impact on tonic immobility, which consequently reduces stress in poultry. By inhibiting distinct developmental stages of Eimeria species, betaine reduces the damaging effects of coccidiosis on broiler chickens and improves intestinal structure and function. The immunological, cardiovascular, neurological, renal, and hepatic metabolic systems benefit from betaine's osmo-protective properties. Therefore, betaine has the potential to be considered as an alternative to feed additives and enhances the health status and productive performance of poultry.

The effect of a postbiotic produced by stabilized non-viable Lactobacilli on the health, growth performance, immunity, and gut status of colisepticaemic broiler chickens.

This work was designed to evaluate the efficacy of a postbiotic compound produced by stabilized non-viable Lactobacilli on the health, growth performance, immunity, and gut status against Escherichia coli (E. coli) challenge of broiler chickens. A total of 400, day-old broiler chicks were allocated into 4 equal groups (1-4) consisting of 100; each assigned into 2 equal replicates (50 each). Chickens in the 1st group were received the dry form of the compound at doses of 1 kg and 0.5 kg/ton feed for starter and grower, and the finisher diets, respectively. Chickens in the 2nd group were given the aqueous form of the compound in a dose of 4 mL/L of the drinking water during the first 3 days of life and at a day before and after each vaccination. Feed and water treatment regimens were administered to chickens in the 3rd group. Group 4 was kept without treatment. Each bird in the 1st, 2nd, 3rd, and 4th group was challenged with E. coli (O78) at 1-week-old. All groups were kept under observation till 5-week-old. Statistical analysis included one-way ANOVA and other methods as described with significant differences at P ≤ 0.05. The results indicated that feed and water treatments with the postbiotic compound induced more significant (P ≤ 0.05) amelioration of a disease picture, enhancement of growth performance, boosting of immune response, improvement of bursa of Fabricius/body weight ratio, and reduction of intestinal coliform count in challenged chickens when compared with challenged non-treated chickens. In conclusion, the postbiotic compound either in a dry and/or an aqueous form is recommended for improving the health, performance, and immunity of colisepticaemic broiler chickens.

Sequence Analysis, Antibiogram Profile, Virulence and Antibiotic Resistance Genes of XDR and MDR Gallibacterium anatis Isolated from Layer Chickens in Egypt.

Background: Gallibacterium anatis is incriminated frequently in severe economic losses and mortalities in the poultry industry. This study aimed to detect the prevalence of G. anatis in layer chickens, sequence analysis, the antibiogram profiles, and PCR screening of virulence determinants and antibiotic resistance genes. Methods: Accordingly, 300 samples (tracheal swabs, ovary and oviduct, and lung) were randomly collected from 100 diseased layer chickens from private commercial layer farms at Elsharkia Governorate, Egypt. The bacteriological examination was carried out. The retrieved isolates were tested for 16S rRNA-23S rRNA gene sequencing, antibiogram profiling, PCR screening of virulence (gtxA, fifA, and gyrB), and antibiotic resistance genes (bla ROB, aphA1, tetB, and tetH). Results: The prevalence of G. anatis was 25% in the examined diseased layer chickens. The sequence analyses emphasized that the tested strains derived from a common ancestor and exhibited a notable genetic similarity with other G. anatis strains from USA, China, and Denmark. The isolated G. anatis strains were highly resistant to sulfamethoxazole-trimethoprim, oxytetracycline, penicillin, ampicillin, kanamycin, neomycin, and erythromycin. The PCR revealed that the retrieved G. anatis strains carried gtxA, gyrB, and fifA virulence genes with a prevalence of 100%, 100%, and 38.3%, respectively. Approximately 30.1% of the retrieved G. anatis isolates were XDR to six antimicrobial classes and harbored bla ROB, aphA1, and tetB resistance genes. Moreover, 20.5% of the isolated G. anatis strains were MDR to three different classes and carried bla ROB and tetH resistance genes. Conclusion: Briefly, this study emphasized the existence of XDR and MDR G. anatis strains in poultry. Florfenicol and norfloxacin displayed a promising antimicrobial effect against the emerging XDR and MDR G. anatis in poultry. The emergence of XDR and MDR G. anatis is considered a public health alarm.

Comparative efficacy of postbiotic, probiotic, and antibiotic against necrotic enteritis in broiler chickens.

Prevention of necrotic enteritis (NE), caused by Clostridium perfringens (C. perfringens), is one of the most important goals to improve the profitability of broiler chickens. This work aimed to compare the efficacy of 2 antibiotic alternatives including a postbiotic (dry feed additive and aqueous nonviable Lactobacillus (L.) species fermentation) and a probiotic (dry feed additive and aqueous Bacillus (B.) subtilis and B. lischeniformis mixture) with an antibiotic (amoxicillin in water) against NE. Four hundred, day-old broiler chicks were divided into 8 equal groups (Gs), n = 50 each (5 replicates; 10 each). Chickens of G1 (postbiotic dry-feed additive), G2 (postbiotic and antibiotic in drinking water), G3 (postbiotic dry and aqueous), G4 (probiotic dry-feed additive), G5 (probiotic and antibiotic in drinking water), G6 (probiotic dry and aqueous), and G7 (nontreated) were orally inoculated with a toxigenic C. perfringens type A on the d 19 to 21 of age and predisposed with 3X coccidial vaccine for induction of NE. However, chickens of G8 were kept nontreated or challenged. The severity of NE signs was markedly decreased in G3 in comparison with other challenged treatment groups, and the mortality rates were 22%, 10%, 16%, 22%, 12%, 20%, and 36% in Gs 1, 2, 3, 4, 5, 6, and 7, respectively. The best significant (P ≤ 0.05) feed conversion ratio was detected in G3 (1.51), G6 (1.54), and G2 and G8 (1.61). In addition, the European production efficiency factor was significantly (P ≤ 0.05) improved in G3 (279.33) and G2 (266.67), but it was decreased in G7 (177.33) when compared with G8 (339.33). An improvement in intestinal and hepatic pathology and liver function tests, as well as a significant (P ≤ 0.05) decrease in bacterial counts were observed in Gs 2, 5, 3, 6, 1, and 4, respectively in comparison with G7. Immunologically, the highest significant (P ≤ 0.05) hemagglutination inhibition antibody titers for Newcastle disease virus vaccine were in Gs 1 and 3 (6.4 log2). In conclusion, the combined feed and water postbiotic treatment demonstrated promising results in ameliorating the severity of NE and improving the hepatic and the immune status of broiler chickens when compared with the commonly used probiotic and antibiotic.

Mange in Rabbits: An Ectoparasitic Disease with a Zoonotic Potential.

Mange in rabbits is a very important parasitic disease causing high losses. The disease is caused mainly by Sarcoptes scabiei, Psoroptes cuniculi, Cheyletiella parasitovorax, and Notoedres cati. Body mange and ear mange are the most common forms of this disease in rabbits. Animals can get mite infestation through direct contact with infected animals or contaminated fomites. This infestation is characterized by zoonotic nature and public health burden. The skin affection is characterized by pruritus, alopecia, severe cachexia, and sometimes death. Infestation is diagnosed mainly by skin scraping and microscopic examination. Control measures mainly depend on the use of different types of systemic and topical acaricides and the use of natural products and supportive elements. Vaccine is not commercially available and is still under investigation. Accordingly, this review article was designed to shed the light on the mange disease in rabbits in terms of mite's infestation and susceptibility, clinical manifestations, zoonosis, diagnosis, and control strategies.

An updated insight into the gastrointestinal helminthoses of poultry: a review.

This review article provides more information about the incidence of helminths affect the gastrointestinal tracts of poultry in different countries, life cycle, clinical picture, diagnosis, and prevention and control measures of such infections. Backyard and deep litter production systems show higher helminth infections than cage system. Moreover, the incidence of helminth infection is more common in tropical countries of Africa and Asia than of European ones due to the suitability of environment and management conditions. Nematodes and cestodes are the most common gastrointestinal helminths of avian species, followed by trematodes. The life cycles of helminths may be direct or indirect, but the infection is usually through faecal-oral route. Affected birds show general signs, low production performance parameters, and even death due to intestinal obstruction and rupture. Lesions of the infected birds reveal catarrhal to haemorrhagic enteritis according to the severity of infection. Diagnosis of affection is mainly based on post mortem examination or microscopic detection of eggs or parasites. As internal parasites adversely affect the host causing poor feed utilization and low performance, thus intervention control strategies are urgent. Prevention and control strategies are relied on application of strict biosecurity measures, eradication of intermediate hosts, early routine diagnosis, and continuous application of specific anthelmintic drugs. Deworming using herbal medicine is recent and successful and may be good alternative to chemicals. In conclusion, helminth infections of poultry remain a major hurdle against the profitable production in poultry producing countries and necessary preventive and control measures should be strictly applied by poultry producers.

Pseudomonas aeruginosa infection of avian origin: Zoonosis and one health implications.

Zoonotic diseases are diseases that are transmitted from animals to humans and vice versa. Pseudomonas aeruginosa (P. aeruginosa) is a pathogen with zoonotic nature. Commercial poultry could be infected with P. aeruginosa, especially at young ages with great losses. Infection of embryos with P. aeruginosa induced death in the shell, while infection of chicks led to septicemia, respiratory and enteric infections, and high mortality. Humans are also highly susceptible to P. aeruginosa infection, and the disease is associated with severe lung damage, especially in immunocompromised patients. Chicken carcass and related poultry retail products play an important role in the transmission of P. aeruginosa to humans, especially after processing in abattoirs. Treatment of P. aeruginosa infection is extremely difficult due to continuous development of antibiotic resistance. The transfer of antibiotic-resistant genes from poultry products to humans creates an additional public health problem. Accordingly, this study focused on avian pseudomonad, especially P. aeruginosa, with respect to infection of poultry, transmission to humans, and treatment and antibiotic resistance.

Paraprobiotics and postbiotics: Contemporary and promising natural antibiotics alternatives and their applications in the poultry field.

With the high rise of drug resistance in microbial populations, there has been a surge in researches to find new natural antibiotics alternative compounds that can be used safely in both humans and animals. The main goals of using this category of alternatives are maintaining the gut microbiome in healthy conditions and preventing the attachment of pathogenic organisms at the early life stages. Probiotics, prebiotics, and synbiotics have been widely used for several years as growth promoters and as preventive measures against several enteric pathogens with successful results. Recently, paraprobiotics and postbiotics are derivatives of probiotic cultures and have been used in humans, animals, and poultry. They are regarded as immunostimulators, anti-inflammatory, antioxidants, and anti-microbial, as well as growth promoters. Till now, there is scanty information about the use of paraprobiotics and postbiotics in animals or in the poultry sector. Accordingly, this review article has focused on defining these new categories of natural alternatives with descriptions of their types, functions, and uses, especially in the poultry field.

Helicobacter pullorum: A potential hurdle emerging pathogen for public health.

Emerging zoonotic pathogens gain more attention due to the adverse effects on human and animal's health and productivity. One of these zoonotic pathogens is Helicobacter pullorum (H. pullorum) which was firstly diagnosed in 1994. This bacterium is enterpathogenic in poultry and contaminates the carcasses meat during processing or improper handling. Human can get H. pullorum infection mainly through mishandling of contaminated carcasses or consumption of undercooked meat. Infection of H. pullorum in human is associated with gastroenteritis and hepatitis. Diagnosis of H. pullorum is very difficult as misdiagnosis with other enteric zoonotic pathogens like Campylobacter and other Helicobacter species is common. Unlike other types of Helicobacter, there are little information and few researches regarding prevalence, pathogenesis, diagnosis and control of H. pullorum infection either animals or human. Accordingly, this review article was prepared to give more details about H. pullorum sources of infection, pathogenicity, incidence in poultry and human as well as its treatment.

Salmonellosis: A food borne zoonotic and public health disease in Egypt.

Salmonellosis is an important food borne disease of public health significance. Global estimates of the disease burden shows more than 20 million cases and 0.15 million deaths annually. The disease caused by a variety of Salmonella organisms worldwide. Salmonella pathogens are belonging to family Enterobacteriaceae that are known to infect many hosts inducing variable clinical diseases pictures. Typhoidal and non-typhoidal Salmonellae are common diseases among Egyptians with severe socioeconomic losses. Different species of animals and poultry as well as their products are the main sources and reservoirs for zoonotic human illness. Enteric fever and gastroenteritis are the main clinical manifestations in patients. Great attention toward salmonellosis drug resistance, prevention and control should be considered.

One health approach of campylobacteriosis in Egypt: An emerging zoonotic disease.

World Health Organization (WHO) defined zoonotic diseases as diseases transmitted from animals to humans and vice versa with or without vector, where more than 75% of the human's diseases have been transmitted from animals or their products. Nevertheless, campylobacteriosis is still one of the most important food borne zoonotic diseases that is likely to challenge global public health all over the world. In Egypt, campylobacteriosis causes severe losses in comparison with other food borne pathogens like Salmonella or Escherichia coli. The potential sources of Campylobacter transmission are poultry backyards and meat, cattle meat, raw milk and water. The main clinical manifestations of human's campylobacteriosis are gastroenteritis and later on nervous signs. Prevention and control strategic plans of campylobacteriosis are very crucial.