RESUMO
Chile, the world's leading copper producer, generates significant volumes of mining waters, some of which cannot be recirculated into the production process. These mining waters are characterized by elevated sulfate (SO42-) concentrations, requiring sustainable management strategies for potential reuse. This study aims to evaluate the rhizofiltration technique using Carpobrotus chilensis for treating mining waters with a high SO42- concentration. Initially, the mining waters exhibited a pH of 7.97 ± 0.16 and a SO42- concentration of 2,743 ± 137 mg L-1, while the control water had a pH of 7.88 ± 0.08 and a SO42- concentration of 775 ± 19.0 mg L-1. The plants were hydroponically cultivated in 40 L containers with mining waters and drinking water as a control. Over an 8-week period, the pH of the mining water decreased to 3.12 ± 0.01, and the SO42- concentration declined to 2,200 ± 110 mg L-1. Notably, the fresh weight of roots was significantly higher in plants grown in mining water (22.2 ± 6.66 g) compared to those in the control treatment (14.3 ± 4.28 g). However, an undesirable increase in the acidity was observed in the mining waters after rhizofiltration, which was attributed to hydrogen sulfate (HSO4-) and/or root exudates. Despite the unexpected increase in acidity, C. chilensis effectively reduced the concentration of SO42- in mining waters by 20%. Additionally, the C. chilensis roots accumulated 4.84 ± 1.40% of sulfur (S), a level comparable to thiophore plants. This study provides evidence that this non-aquatic plant can be used in sulfate rhizofiltration.
Caprobrotus chilensis is a good candidate for sulfate rhizolfiltration in mining waters.The accumulation of sulfur by the roots of Carpobrotus chilensis reached 4.84%Mining waters with a high concentration of sulfates require control of the redox potential.
Assuntos
Biodegradação Ambiental , Mineração , Sulfatos , Poluentes Químicos da Água , Sulfatos/metabolismo , Poluentes Químicos da Água/metabolismo , Filtração , Purificação da Água/métodosRESUMO
The chemical characteristics of mine tailings, organic amendments (doses), and plants are the critical factors that must be evaluated and monitored to ensure the sustainability of phytostabilization. The aim of this study was to evaluate the mobility of copper (Cu) in mine tailings (MT) of the Zone Central of Chile to which commercial humic substances were added, examining their effect on the uptake of Atriplex halimus. Two commercial humic substances (HS1 and HS2) extracted from leonardite (highly oxidized lignite), of different pH and total organic carbon, were evaluated by adsorption curve for Cu. In columns, soluble Cu, pH, and electrical conductivity in leachates were evaluated for MT, MT + HS1, and MT + HS2, and HS1 and HS2 in doses of 120 mg kg-1. In pot assay, seeds were germinated directly in MT and cultivated for 140 days with the addition of HS2 in 120 and 240 mg kg-1. Mine tailing presents high concentration of Cu (2016 ± 223 mg kg-1, pH 6.3 ± 0.1). The results of sequential extraction indicate that Cu is associated with the sulfide fraction of low risk of mobility. The amount of Cu sorbed by HS1 was higher than that sorbed by HS2, and both humic substances showing better fit to the Freundlich than Langmuir model. Lixiviation of Cu was significantly lower in MT + HS1 (0.166 ± 0.043 mg kg-1) and MT + HS2 (0.157 ± 0.018 mg kg-1) than in MT (0.251 ± 0.052 mg kg-1). Copper concentration in plants reached 185.8 ± 37.8 mg kg-1 in the roots and 32.6 ± 7.4 mg kg-1 in the aerial parts cultivated in MT without effect of the humic substance addition in Cu uptake nor growth. Copper concentrations in the aerial parts were adjusted to sufficient or normal levels in plant. A good management of mine tailings through phytostabilization could consider an adequate mixture of humic substances (to avoid leaching of metals) and an organic amendment that provides essential nutrients and increases biomass generation.
Assuntos
Atriplex/química , Atriplex/metabolismo , Monitoramento Ambiental/métodos , Substâncias Húmicas/análise , Poluentes do Solo/análise , Solo/química , Adsorção , Biodegradação Ambiental , Biomassa , Chile , Cobre/análise , Minerais/química , Mineração , Plantas/químicaRESUMO
In the ChiuChiu village (Atacama Desert, Chile), there is a high concentration of arsenic (As) in the soil due to natural causes related to the presence of volcanoes and geothermal activity. To compare the levels of As and the growth parameters among plants of the same genus, three species of plants were established in situ: Atriplex atacamensis (native of Chile), Atriplex halimus, and Atriplex nummularia. These soils have an As concentration of 131.2 ± 10.4 mg kg(-1), a pH of 8.6 ± 0.1, and an electrical conductivity of 7.06 ± 2.37 dS m(-1). Cuttings of Atriplex were transplanted and maintained for 5 months with periodic irrigation and without the addition of fertilizers. The sequential extraction of As indicated that the metalloid in these soils has a high bioavailability (38 %), which is attributed to the alkaline pH, low organic matter and Fe oxide content, and sandy texture. At day 90 of the assay, the As concentrations in the leaves of A. halimus (4.53 ± 1.14 mg kg(-1)) and A. nummularia (3.85 ± 0.64 mg kg(-1)) were significantly higher than that in A. atacamensis (2.46 ± 1.82 mg kg(-1)). However, the three species accumulated higher levels of As in their roots, indicating a phytostabilization capacity. At the end of the assay, A. halimus and A. nummularia generated 30 % more biomass than A. atacamensis without significant differences in the As levels in the leaves. Despite the difficult conditions in these soils, the establishment of plants of the genus Atriplex is a recommended strategy to generate a vegetative cover that prevents the metalloid from spreading in this arid area through the soil or by wind.
Assuntos
Arsênio/análise , Atriplex/fisiologia , Biodegradação Ambiental , Clima Desértico , Poluentes do Solo/análise , Solo/química , Biomassa , Chile , Meio Ambiente , Monitoramento Ambiental , Fertilizantes , Folhas de Planta/química , Raízes de Plantas/químicaRESUMO
El presente trabajo se propone relacionar el funcionamiento del superyó y la psicosis. Partiendo de los hechos clínicos que prefiguran e imponen la noción de superyó, y hasta su conceptualización en 1923, fecha en que encuentra su forma acabada en la teoría, se tratará de mostrar cómo los procesos psíquicos que Freud describe bajo este término lo llevan del lado de la psicosis. El caso del Hombre de las Ratas ofrecerá la oportunidad de entrar en contacto directo con el superyó: al hacer el balance del sentimiento de culpabilidad y la conciencia moral, se tomará nota de que esta instancia no se aviene a la diferencia entre consciente e inconsciente. Se concluirá que el superyó manifiesta las características mayores del inconsciente, salvo que a cielo abierto, como se dice
This paper proposes to link the functioning of the superego and psychosis. Based on the clinical facts that prefigure and impose the notion of superego, and even its conceptualization in 1923, when it finds its way finished in theory, will try to show how mental processes that Freud describes under this term takes at the side of the psychosis. The case of the Rat Man will offer the opportunity to come into direct contact with the superego: at the moment to make a balance stock of guiltiness and the moral conscience, will take note that this instance does not agree to the difference between conscious and unconscious. It will then clear that the superego manifests the greatest features of the unconscious, unless the opencast, as it is said
Assuntos
Humanos , Masculino , Adulto , Psicanálise , Transtornos Psicóticos , Comportamento Obsessivo , Transtorno Obsessivo-Compulsivo , Inconsciente PsicológicoRESUMO
We examined the metazoan parasite fauna of the bigeye flounder, Hippoglossina macrops, in a latitudinal gradient of the southeastern Pacific (ca. 25 degrees S-33 degrees S) to assess whether their parasites show latitudinal and/or biogeographical patterns, and to determine if these patterns agree well with those observed for free-living organisms. In total, 331 fish from 6 localities were analyzed. Eighteen parasite species, including 5 ecto- and 13 endoparasites, were found in the bigeye flounder. Neoheterobothrium chilensis, Floridosentis sp., and Corynosoma australe were the most prevalent parasite species. Ecto- and endoparasites of H. macrops showed different latitudinal patterns. Only endoparasite species show a distributional pattern concordant with biogeographic areas recognized for free-living organisms; this finding was supported by cluster analyses showing 2 major divisions. The first joined the most northern localities (25 degrees S-26 degrees S), and the second consisted of 2 subgroups: (1) 28 degrees S-30 degrees S, and (2) 31 degrees S-32 degrees S. A multivariate analysis of infracommunities showed significant differences in the parasitic load of H. macrops from different latitudes. The latitudinal differences in the prevalence and abundance of the parasites might be explained by differing oceanographic conditions in water masses. These physical conditions could affect the dispersal ability of the infective stages of ecto-parasites, and produce zoogeographical breaks that could affect the distribution of intermediate hosts (invertebrate and vertebrate prey), subsequently affecting the transmission of endoparasites.
Assuntos
Ectoparasitoses/veterinária , Doenças dos Peixes/epidemiologia , Linguados/parasitologia , Enteropatias Parasitárias/veterinária , Animais , Biodiversidade , Chile/epidemiologia , Análise por Conglomerados , Análise Discriminante , Ectoparasitoses/epidemiologia , Ectoparasitoses/parasitologia , Feminino , Doenças dos Peixes/parasitologia , Enteropatias Parasitárias/epidemiologia , Enteropatias Parasitárias/parasitologia , Masculino , Oceano Pacífico , PrevalênciaRESUMO
A program to calculate the neutron KERMA in human tissues has been developed. The program was developed in Mathcad and contains the neutron kerma factors of those elements that are present in different human tissues. Having the elemental composition of any human tissue the neutron kerma can be easily calculated. The program was tested using the elemental composition of tumor tissues such as sarcoma, melanoma, carcinoma and adenoid cystic. Neutron kerma for adipose and muscle tissue for normal adult was calculated. The results are in agreement with those published in literature. The neutron kerma for water was also calculated because in some dosimetric calculations water is used to describe normal and tumor tissues. From this comparison was found that at larger energies kerma factors are approximately the same, but energies less than 100 eV the differences are large.
Assuntos
Humanos , Neoplasias/química , Nêutrons , Radiação de Fundo , Tecido Conjuntivo/química , AlgoritmosRESUMO
Artificial Neural Network Technology has been applied to unfold neutron spectra and to calculate 13 dosimetric quantities using seven count rates from a Bonner Sphere Spectrometer with a (6)LiI(Eu). Two different networks, one for spectrometry and another for dosimetry, were designed. To train and test both networks, 177 neutron spectra from the IAEA compilation were utilised. Spectra were re-binned into 31 energy groups, and the dosimetric quantities were calculated using the MCNP code and the fluence-to-dose conversion coefficients from ICRP 74. Neutron spectra and UTA4 response matrix were used to calculate the expected count rates in the Bonner spectrometer. Spectra and H(10) of (239)PuBe and (241)AmBe were experimentally obtained and compared with those determined with the artificial neural networks.
Assuntos
Algoritmos , Redes Neurais de Computação , Nêutrons , Exposição Ocupacional/análise , Monitoramento de Radiação/instrumentação , Proteção Radiológica/instrumentação , Análise Espectral/instrumentação , Biotecnologia/instrumentação , Biotecnologia/métodos , Desenho de Equipamento , Análise de Falha de Equipamento , Doses de Radiação , Monitoramento de Radiação/métodos , Proteção Radiológica/métodos , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Análise Espectral/métodosRESUMO
A Monte Carlo study to determine the shielding features to neutrons of water-extended polyester was carried out. During calculations, (252)Cf and shielding were modelled and the neutron spectra as well as the H(10) were calculated in four sites. The calculation was extended to include a water shielding, the source in vacuum and in air. Besides neutron shielding characteristics, the Kerma in air due to gammas emitted by (252)Cf and due to capture gamma rays in the shielding were included.
Assuntos
Califórnio/análise , Nêutrons , Poliésteres/química , Proteção Radiológica/instrumentação , Água/química , Desenho de Equipamento , Análise de Falha de Equipamento , Doses de Radiação , Monitoramento de Radiação/instrumentação , Monitoramento de Radiação/métodos , Proteção Radiológica/métodos , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
A gamma ray spectrometer, with a 3('') Ø X 3('') NaI(Tl) detector, with a moderator sphere has been utilised to measure the neutron fluence rate, with this value the H(10) was estimated. When a neutron is captured by the hydrogen-based moderator, a 2.22 MeV prompt gamma ray is produced. In a multichannel analyser the net area under the 2.22 MeV photopeak is proportional to the total neutron fluence rate. The features of this system were determined by a Monte Carlo study that includes 3-, 5- and 10-inches diameter, water and polyethylene moderators and a (239)Pu-Be source. The prompt gamma response was extended to monoenergetic neutron sources. To verify the response, a (239)Pu-Be source in combination with a 10('') polyethylene sphere having a gamma-ray spectrometer with NaI(Tl) was utilised to estimate the neutron fluence rate and the H(10). These results were compared with neutron fluence rate and H(10) obtained using a Bonner sphere spectrometer and with the H(10) measured using a neutron remmeter.
Assuntos
Desenho Assistido por Computador , Nêutrons , Monitoramento de Radiação/instrumentação , Proteção Radiológica/instrumentação , Espectrometria gama/instrumentação , Desenho de Equipamento , Análise de Falha de Equipamento , Método de Monte Carlo , Doses de Radiação , Monitoramento de Radiação/métodos , Proteção Radiológica/métodos , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Espectrometria gama/métodosRESUMO
Mediante una serie de medidas y cálculos Monte Carlo se han determinado las características dosimétricas y los espectros de los fotoneutrones que se producen en torno a un acelerador lineal para radioterapia de 18 MV. Las medidas se realizaron con dosímetros termoluminiscentes TLD 600 y TLD 700 que se expusieron desnudos y emparedados con Cd, así como dentro de una esfera de parafina y dentro de esferas Bonner.
Measurements and Monte Carlo calculations has been utilized to determine the dosimetric features as well as the neutron spectra of photoneutrons produced around an 18 MV linear accelerator for radiotherapy. Measurementes were carried out with bare and Cd covered thermolumiscent dosimeters, TLD600 and TLD700, as well as inside a paraffine moderator. TLD pairs were also utilized as thermal neutrons inside a Bonner sphere spectrometer.
Assuntos
Humanos , Aceleradores de Partículas , Fótons , Nêutrons , Radioterapia/instrumentação , Algoritmos , Análise Espectral , Dosagem Radioterapêutica , Dosímetros de Radiação , Método de Monte Carlo , Tecnologia RadiológicaRESUMO
An artificial neural network (ANN) has been designed to obtain neutron doses using only the count rates of a Bonner spheres spectrometer (BSS). Ambient, personal and effective neutron doses were included. One hundred and eighty-one neutron spectra were utilised to calculate the Bonner count rates and the neutron doses. The spectra were transformed from lethargy to energy distribution and were re-binned to 31 energy groups using the MCNP 4C code. Re-binned spectra, UTA4 response matrix and fluence-to-dose coefficients were used to calculate the count rates in the BSS and the doses. Count rates were used as input and the respective doses were used as output during neural network training. Training and testing were carried out in the MATLAB environment. The impact of uncertainties in BSS count rates upon the dose quantities calculated with the ANN was investigated by modifying by +/-5% the BSS count rates used in the training set. The use of ANNs in neutron dosimetry is an alternative procedure that overcomes the drawbacks associated with this ill-conditioned problem.
Assuntos
Algoritmos , Modelos Biológicos , Redes Neurais de Computação , Nêutrons , Monitoramento de Radiação/métodos , Proteção Radiológica/métodos , Análise Espectral/métodos , Carga Corporal (Radioterapia) , Simulação por Computador , Humanos , Modelos Estatísticos , Doses de Radiação , Monitoramento de Radiação/instrumentação , Eficiência Biológica Relativa , Análise Espectral/instrumentaçãoRESUMO
La hiperplasia prostática benigna (HPB) es una patología de alta prevalencia en la consulta urológica. Una herramienta efectiva de tratamiento es la cirugía abierta transvesical, que en nuestro Servicio se indica principalmente a pacientes con volúmenes prostáticos superiores a 50 gramos. El objetivo de este estudio es conocer el manejo de los pacientes con HPB con indicación de cirugía abierta. Para esto, se efectúo un análisis retrospectivo de 328 fichas clínicas de pacientes intervenidos quirúrgicamente entre agosto de 1999 a julio de 2002. Dentro del estudio, el promedio de edad fue 68 años. El antígeno prostático específico (APE) promedio fue de 7,22 ng/ml, con antígeno menor a 4 ng/ml en el 41 por ciento y mayor a 10 ng/ml en el 19 por ciento. Dentro del estudio diagnóstico se practicó biopsia prostática transrectal ecodirigida al 32 por ciento. El volumen prostático promedio fue de 79,2 gramos, con un rango de 33 a 190 gramos. El principal motivo de consulta fue la sintomatología obstructiva baja (86 por ciento). Al momento de la cirugía, un 52 por ciento portaba sonda Foley uretrovesical. El 66 por ciento de las cirugías fueron realizadas por médicos residentes, asesorados por urólogo del servicio. Las complicaciones intra operatorias fueron 2,7 por ciento, siendo la principal el desgarro capsular. La sonda se retiró a los 6,3 días, con un promedio de estadía hospitalaria postoperatoria de 7,4 días. En el seguimiento, se destaca infección de herida operatoria (7,9 por ciento), hematuria (2,7 por ciento), infección del tracto urinario (ITU) (2,7 por ciento). El 1,2 por ciento fue reintervenido, principalmente por hemovejiga. Se encontró en forma incidental, cáncer prostático en 1,2 por ciento de los casos. No se registró mortalidad en esta revisión. El abordaje quirúrgico transvesical constituye la vía de elección en pacientes con volúmenes prostáticos superiores a 50 gramos. Es una técnica segura y presenta pocas complicaciones intra operatorias. Como complicación tardía más frecuente se presentó la infección de herida operatoria, no siendo significativo el uso de sonda Foley a permanencia en el preoperatorio. Las demás complicaciones están bajo los estándares mundiales esperados. En comparación con la literatura internacional, llama la atención el bajo porcentaje de adenocarcinoma prostático diagnosticado en forma incidental, a pesar de un APE promedio elevado.
Assuntos
Humanos , Masculino , Adulto , Pessoa de Meia-Idade , Estatísticas Hospitalares , Hiperplasia Prostática/cirurgia , Prostatectomia/estatística & dados numéricos , Antígeno Prostático Específico , Hiperplasia Prostática/diagnóstico , Hiperplasia Prostática/epidemiologia , Hiperplasia Prostática/patologia , Prostatectomia/efeitos adversos , Prostatectomia/métodos , Estudos Retrospectivos , Reoperação/estatística & dados numéricos , Tempo de Internação/estatística & dados numéricosRESUMO
La Hiperplasia Prostática Benigna (HPB), es una de las patologías de mayor prevalencia en la consulta urológica diaria. Una herramienta efectiva de tratamiento es la cirugía endoscópica, la que se lleva a cabo mediante la Resección Transuretral (RTU) de los lóbulos prostáticos hiperplásicos. Se realizó un muestreo simple retrospectivo documental de fichas clínicas de pacientes sometidos a cirugía endoscópica con diagnóstico de HPB en el período enero 2000 - julio 2002. En el período evaluado se realizaron 107 RTU, el 74 porciento de los pacientes era mayor de 60 años. El antígeno prostático específico (APE) preoperatorio fue menor de 4 ng/ml en el 63 porciento de los casos y sólo un 6,5 porciento presentó APE mayor a 10 ng/ml. En el 56 porciento de los casos el volumen prostático fluctuó entre 30 y 50 grs. El principal motivo de consulta fue la presencia de sintomatología del tracto urinario bajo con un 95 porciento. En la primera consulta en policlínico, un 28 porciento de los casos ya venía con sonda Foley a permanencia por retención aguda de orina (RAO). Al momento de la cirugía, el 43 porciento estaba con RAO. El 60 porciento de las cirugías fueron realizadas por los becados del servicio. Se realizaron 3 conversiones a cirugía abierta. Una por lesión capsular, otra por falsa vía y otra por hemovejiga. El 71 porciento de los pacientes requirió menos de 5 días de estada con sonda Foley. Un 10 porciento de los pacientes presentó infección urinaria postoperatoria. La RTU es una buena alternativa quirúrgica en pacientes con HPB pequeñas, con escasas complicaciones intraoperatorias. El elevado porcentaje de infecciones urinarias puede ser explicado por el alto número de pacientes portadores de sonda Foley a permanencia en el preoperatorio.
Assuntos
Humanos , Masculino , Adulto , Pessoa de Meia-Idade , Endoscopia/métodos , Hiperplasia Prostática/cirurgia , Ressecção Transuretral da Próstata/métodos , Distribuição por Idade , Antígeno Prostático Específico , Hiperplasia Prostática/diagnóstico , Complicações Intraoperatórias , Infecções Urinárias/etiologia , Complicações Pós-Operatórias , Estudos Retrospectivos , Reoperação/estatística & dados numéricos , Ressecção Transuretral da Próstata/efeitos adversos , Amostragem Aleatória SimplesRESUMO
Linfocitos humanos fueron irradiados en un campo de radiación gamma de baja intensidad para determinar la expresión de las proteínas de choque calórico en función de la dosis. Los linfocitos fueron obtenidos de individuos cuyo trabajo los identifica como ocupacionalmente expuesto y no ocupacionalmente expuestos. La identidad de las proteínas se realizó utilizando anticuerpos contra las proteínas Hsp25, Hsp60, Hsp70 y Hsp90. De éstas, solamente la proteína hsp70 fue detectada antes y después de la irradiación. Los linfocitos del personal ocupacionalmente expuesto y no ocupacionalmente expuesto expresaron, antes y después de la irradiación, solamente la proteína Hsp70. La cantidad de proteína resultó directamente proporcional al tiempo de irradiación. Después de una dosis gamma de 70.5 mGy, los linfocitos del individuo ocupacionalmente expuesto expresaron una mayor cantidad de proteína Hsp70 que la expresada por los linfocitos del personal no ocupacionalmente expuesto. Este hecho es indicio de que el individuo ocupacionalmente expuesto tiene una mayor tolerancia a los rayos gamma (gamma-tolerancia), inducida por un proceso de adaptación generada por su condición laboral
Assuntos
Humanos , Masculino , Adulto , Pessoa de Meia-Idade , Linfócitos/efeitos da radiação , Raios gama/efeitos adversos , Radiação Ionizante , Proteínas de Choque Térmico HSP90/efeitos da radiação , Proteínas de Choque Térmico HSP70/efeitos da radiação , Chaperonina 60/efeitos da radiação , Exposição Ocupacional , Tolerância a RadiaçãoRESUMO
The metazoan parasite fauna of Hippoglossina macrops (n = 123) from northern Chile (30 degrees S) is quantitatively described for the first time, and the role of host age and sex was evaluated. Twelve parasite species were recovered, including 5 ectoparasites (2 Monogenea, 2 Copepoda and 1 Piscicolidae) and 7 endoparasites (1 Digenea, 3 Cestoda, 2 Acanthocephala, and 1 Nematoda). The copepod Holobomolochus chilensis, the monogenean Neoheterobothrium sp., the adult acanthocephalan Floridosentis sp. and the hirudinean, Gliptonobdella sp. are new geographical and host records. The most prevalent ectoparasitic species were the monogenean, Neoheterobothrium sp. and the copepod, H. chilensis. Among endoparasites, the acanthocephalans Floridosentis sp. and Corynosoma australe were most prevalent and abundant. Prevalence and mean intensity of infection for most parasitic species were not affected by host sex, however the prevalence of Floridosentis sp. was significantly greater in males. Intensity of infection was positively correlated with host age for Neoheterobothrium sp., and negatively correlated for Floridosentis sp. and H. chilensis. The helminth species richness of the host H. macrops was lower compared to related flatfishes from the Northern Hemisphere. The relationship of the helminth fauna of H. macrops, its feeding habits and ecological habitats are discussed.
Assuntos
Linguados/parasitologia , Fatores Etários , Animais , Chile , Feminino , Interações Hospedeiro-Parasita/fisiologia , Masculino , Parasitos/fisiologia , Fatores SexuaisRESUMO
The relationship between host size and sex and the abundance, prevalence, species richness, diversity, and total number of parasites in the infracommunities of Sebastes capensis was analyzed. Nine helminth species were obtained from 180 red rockfish captured off Coquimbo Bay, Northern Chile. These included the digeneans Pseudopecoelus sp., Diphtherostomum sp., Derogenes sp., Podocotyloides sp., and Lecithochirium sp., the acanthocephalan Corynosoma sp., and the nematodes Ascarophis sebastodis, Hysterothylacium sp., and Anisakis sp. The abundances of Pseudopecoelus sp. and Anisakis sp. were significantly and positively correlated with the size of S. capensis, while Corynosoma sp. correlated negatively with host size. Only prevalence of Anisakis sp. increased with host size. The number of parasite individuals increased significantly with size of S. capensis. However, species richness and diversity of the infracommunities was not affected by host size. These results suggest that red rockfish > 18 cm do not undergo significant ontogenetic changes in their diet. Host sex did not seem to affect abundance and prevalence of infections. The presence of Derogenes sp. and Podocotyloides sp. are new records of digeneans for S. capensis.
Assuntos
Doenças dos Peixes/parasitologia , Helmintíase Animal/parasitologia , Helmintos/crescimento & desenvolvimento , Análise de Variância , Animais , Constituição Corporal , Chile/epidemiologia , Feminino , Doenças dos Peixes/epidemiologia , Peixes , Helmintíase Animal/epidemiologia , Helmintos/classificação , Helmintos/isolamento & purificação , Masculino , Prevalência , Fatores Sexuais , Vísceras/parasitologiaRESUMO
OBJECTIVE: To test in vitro the feasibility of welding amniotic membranes using Nd:YAG laser energy. STUDY DESIGN: Fresh fetal membranes from term pregnancies were washed and cut into 1 cm2 pieces. Pooled cryoprecipitate (CPT), 50% albumin (Alb), or polytetrafluoroethilene (e-PTFE) were used as solder medium. The optimal settings of the laser were determined. Results were assessed quantitatively and semi-quantitatively using Pearson Chi-square analysis. RESULTS: Laser welding of amniotic membranes was successful in 82.6% of experiments with e-PTFE and in 10.7% of experiments with CPT (P < 0.001). The strength of the welding was also significantly better with e-PTFE (P < 0.001). Optimal results were obtained using 1-7 Watts and 0.1-1 seconds. Laser welding was unsuccessful in 100% of experiments with Alb. CONCLUSIONS: Laser welding of fetal membranes can be accomplished with e-PTFE and to a lesser degree with the CPT using Nd:YAG energy under low wattage-high exposure settings. Further studies are underway to test other grafting or soldering materials.
Assuntos
Membranas Extraembrionárias/cirurgia , Fotocoagulação a Laser , Feminino , Ruptura Prematura de Membranas Fetais/prevenção & controle , Humanos , Técnicas In Vitro , Politetrafluoretileno , GravidezRESUMO
OBJECTIVE: Our purpose was to report the midtrimester Down syndrome screening efficiency of a 2-analyte algorithm, urine beta-core fragment (a metabolite of human chorionic gonadotropin) and nuchal thickness, along with maternal age in a high-risk population undergoing genetic amniocentesis. METHOD: Nuchal thickness, humerus length, and maternal urine beta-core fragment levels were measured prospectively before genetic amniocentesis in 1360 singleton pregnancies, 21 (1.5%) of which had fetal Down syndrome. All analyte levels were expressed as multiples of the normal medians based on biparietal diameter. Backward-stepwise logistic regression was used to determine whether the markers were significant independent predictors of fetal Down syndrome. Matrix analysis was used to calculate an adjusted Down syndrome likelihood ratio for each patient based on the significant screening markers. Multiplication by age-related midtrimester risk gave the adjusted Down syndrome risk. The sensitivity and false-positive rates at different Down syndrome screening thresholds were used to generate a receiver-operator characteristics curve. The area under the curve was used to assess the value of this screening test. RESULTS: On the basis of logistic regression, beta-core fragment level (P 1/60 the sensitivity and false-positive rate for Down syndrome were 85.7% and 4.9%, respectively, when beta-core fragment level, nuchal thickness, and maternal age were used. Correspondence screening values at a risk threshold > 1/150 were 95.2% and 10.8%, respectively. The area under the receiver-operator characteristics curve was 0.9357 (SE = 0. 0137), indicating that the algorithm is excellent for Down syndrome screening. CONCLUSION: In this study, a combination algorithm consisting of nuchal thickness, urine beta-core fragment level, and maternal age had a high screening efficiency for Down syndrome. This algorithm should be investigated as a new option for women at high risk of having a fetus with Down syndrome.
Assuntos
Gonadotropina Coriônica Humana Subunidade beta/urina , Síndrome de Down/diagnóstico , Idade Materna , Pescoço/diagnóstico por imagem , Diagnóstico Pré-Natal , Adolescente , Adulto , Amniocentese , Feminino , Idade Gestacional , Humanos , Cariotipagem , Modelos Logísticos , Pessoa de Meia-Idade , Gravidez , Curva ROC , Fatores de Risco , Ultrassonografia Pré-Natal , alfa-Fetoproteínas/análiseRESUMO
The parasites of the red rockfish Sebastes capensis off northern Chile are described quantitatively for the first time and compared with those of congeneric species of the Northern Hemisphere as well as of other Chilean marine fishes. Sixteen species were recorded, including 8 ectoparasites (2 copepods, 2 isopods, 1 turbellarian, and 3 monogeneans) and 8 endoparasites (2 acanthocephalans, 3 digeneans, and 3 nematodes). The ectoparasites Lepeophtheirus chilensis and Caligus cheilodactylus, and the endoparasites Pseudopecoelus sp. and Corynosoma sp. were predominant. Eighty percent of the fishes harbored 3-6 parasite species. Four parasite genera new to the genus Sebastes were found in S. capensis, which also shares several parasite genera with its congeneric species from other geographic areas. However, in contrast to its congeners, S. capensis exhibits a lower helminth species richness, although when all the metazoan fauna is considered the species number and diversity are similar. When compared with other demersal fishes of the Chilean coast, S. capensis shows a high number of species and high parasite abundance. Diphtherostomum sp. and Gnathia sp. are new generic records for the parasite fauna of Chilean coast fishes and the finding of Paramicrocotyle sp., Neobenedenia melleni, and Interniloculus chilensis in this study represents a new geographical record for these parasites.
Assuntos
Crustáceos/classificação , Ectoparasitoses/veterinária , Doenças dos Peixes/parasitologia , Helmintíase Animal/parasitologia , Acantocéfalos/classificação , Animais , Chile/epidemiologia , Ectoparasitoses/epidemiologia , Ectoparasitoses/parasitologia , Doenças dos Peixes/epidemiologia , Peixes , Helmintíase Animal/epidemiologia , Nematoides/classificação , Oceano Pacífico , Prevalência , Estações do Ano , Trematódeos/classificaçãoRESUMO
OBJECTIVE: Our purpose was to ascertain the screening efficiency of a new midtrimester Down syndrome detection protocol that combines maternal urine testing and ultrasonographic examination. STUDY DESIGN: In a prospective study, beta-core fragment, the stable end product of human chorionic gonadotropin metabolism, was measured in maternal urine. The results were standardized for urine creatinine levels. The study was performed in women undergoing midtrimester genetic amniocentesis (15 to 24 weeks' gestation). Urine beta-core fragment values were expressed as multiples of the normal median for gestational age. The screening performance of a combination of ultrasonographic parameters and urine beta-core values for Down syndrome detection was determined. RESULTS: A total of 511 singleton pregnancies in women undergoing amniocentesis were studied, 18 of the women (3.5%) had a Down syndrome fetus. A urine beta-core fragment level > or = 97th percentile had a sensitivity of 61.1% and a false-positive rate of 3.2%. An abnormal prenatal screen was defined as a urine beta-core level > or = 97th percentile, increased nuchal thickness (> or = 5 mm), or the presence of gross structural defects. Corresponding values for the screening efficiency of an abnormal prenatal screen were sensitivity of 77.8% and a false-positive rate of 4.1%. With an abnormal prenatal screen the odds ratio is 82.8 (95% confidence interval 22.6 to 364.9) for having a Down syndrome fetus. CONCLUSION: The presence of an abnormal maternal urine beta-core level, a gross ultrasonographic anomaly, or increased nuchal thickness had a high detection rate and a low false-positive rate for Down syndrome. This novel screening algorithm is useful for further delineating the risk status in patients at high risk who are reluctant to undergo or decline genetic amniocentesis.