Treatment of masseter muscle hypertrophy with botulinum toxin type A injection: A review of adverse events.

BACKGROUND: The popularity of noninvasive botulinum toxin type A (BTX-A) injections for masseter muscle hypertrophy is increasing among Asian individuals with a square-shaped lower face. AIMS: This study aimed to analyze the adverse events (AEs) caused by BTX-A injections into the masseter muscle. PATIENTS/METHODS: This observational study retrospectively evaluated 46 250 patients who underwent BTX-A injections into the masseter muscle in 2022. The inclusion criteria were the diagnosis of an AE by the physician at the return visit and subsequent follow-up of progress (n = 223). The patients who were lost to follow-up (n = 40) were excluded from the study. RESULTS: Among the 223 patients with AEs, the most common AE was paradoxical bulging (88.3%, n = 197/223). The average period from treatment until confirmation of improvement was 159.6 ± 113.6 days (range 13-667 days) for all AEs, all of which were temporary. The period until improvement was 166.1 days in the intervention group (n = 122) and 151.9 days in the observation group (n = 101) (p = 0.24). As the period until improvement of AEs included the period until the patients visited the clinics and the improvements were confirmed by physicians, the actual period was likely to have been shorter. CONCLUSIONS: (1) All AEs were temporary. (2) All AEs improved within 22.2 months (within 5.3 ± 3.8 months on average). (3) There was no significant difference between the intervention and observation groups in the period until the improvement of AEs.

Lipoaspirate stored at a constant low temperature by electric control suppresses intracellular metabolism and maintains high cell viability.

Background: Cell therapy is a useful treatment method for wide spectrum of diseases which utilizes the immunosuppressive and regenerative abilities of administered cells. It is essential to build a transport system of tissues from which cells are harvested, because various external factors, such as temperature, time, air pressure, and vibration affect the cell functions isolated from body tissues. In particular, temperature is a critical factor which determines the viability of the cells and organs. In this study, we investigated the optimal temperature during the transportation of lipoaspirates from which adipose -derived stem cells (ASCs) were isolated. Method: Lipoaspirates obtained by liposuctions (lipomatic or vaser method) were transported in four different temperature zones (4, 20, 32, and 37 °C) in a transport container which is electrically controlled to maintain a constant temperature during transport. Stromal vascular fractions (SVFs) were harvested from the lipoaspirate, and the cell number, viability and proliferation rate and the yield of ASCs were examined. In addition, the metabolic state of the cells was examined. Results: ASCs from lipoaspirates transported at high temperature significantly decreased cell viability, while those at low temperature maintained high cell viability and showed good cell proliferation. In addition, transportation of lipoaspirates at low temperature resulted in a high level of NAD+/NADH, coenzymes involved in intracellular metabolism, and a low level of lactate in lipoaspirate suppressed the glycolytic system of intracellular metabolism, in ASCs. Conclusion: The lipoaspirate transported at 4 °C exhibited best results regarding live cell number, viability and cell proliferation in our experiments. This study offers a direction to build a transport system that connects laboratories and hospitals and achieve a beneficial therapy for patients.

Current Status of Early Complications Caused by Hyaluronic Acid Fillers: Insights From a Descriptive, Observational Study of 41,775 Cases.

BACKGROUND: The number of hyaluronic acid (HA) filler treatments has increased in recent years. Although extremely rare, serious complications associated with these treatments, such as skin necrosis, blindness, and stroke caused by vascular compromise, have been reported. OBJECTIVES: To evaluate the specific details related to early complications caused by HA filler injection in our group, understand the current status, and gain further insights from the findings. METHODS: A nationwide, observational, descriptive, multicenter, retrospective study was conducted. Of the 41,775 cases (58,533 sites of injection), 29 cases of early complications (onset of less than 14 days after injection) were included in the study. RESULTS: The injection site with the highest rate of early complications was the upper eyelids (0.41%; n = 1/241 sites). The most commonly injected site was the nasolabial fold (n = 13/29 cases), and the most common early complication was vascular compromise (n = 18/29 cases). The average experience of the injectors was 28.7 ± 31.9 months. CONCLUSIONS: All 13 patients injected in the nasolabial fold experienced vascular compromise, potentially related to the anatomical feature of a facial artery running parallel to the nasolabial fold, which is commonly found in Asian populations. Regardless of the injection site, accurate anatomical knowledge and knowledge and experience regarding HA fillers, including appropriate patient selection and injection techniques, are strictly required for injectors to anticipate early complications. Therefore, it is important to establish original guidelines based on experience and ensure their thorough implementation in our facilities.

Quantitative Assessment of the Cryolipolysis Method for Body Contouring in Asian Patients.

PURPOSE: Cryolipolysis has been demonstrated to be a safe and efficient non-invasive treatment modality for reducing subcutaneous discrete areas of fat; however, only limited studies have focused on body contouring in Asian patients. The present work focuses on the quantitative assessment of the cryolipolysis method for body contouring in Asian patients. PATIENTS AND METHODS: The retrospective study evaluated the medical records of 4122 patients with body contouring who underwent cryolipolysis treatment in multiple centers across Japan from January 2019 to June 2019. We evaluated the demographic profiles, treatment areas, and safety assessments among these 4122 patients. The effectiveness of the treatment was assessed in 146 patients at one of the clinics, Shonan Beauty Clinic, out of which 54 and 27 had treatments in the abdomen and upper arm areas, respectively. Clinical outcomes were assessed based on patient surveys, circumference measurements, and assessment of photographs. RESULTS: Evaluation of choice for treatment area suggested that men generally focused more on the surrounding areas of the abdomen, while women even had options for the abdomen and the upper arm. No long-term side effects were observed during the study period. There was a significant reduction in the circumference of both the abdomen and upper arm areas (P < 0.05). Additionally, 16.7% and 18.5% of patients who had received treatment on their abdomen and upper arm, respectively, returned for the next sessions within six months. Patients who had received treatment on their upper arms were more satisfied in the categories of pain and bruising and treatment effectiveness compared to those who had received treatment on their abdomen. CONCLUSION: Based on the results obtained in this retrospective study on Asian patients, it can be stated that cryolipolysis is a safe and well-tolerated nonsurgical fat-reduction procedure. Clinical effectiveness can be consistently achieved with proper patient evaluation and patient satisfaction.