Assessment of irrigation water quality for vegetable farming in peri-urban Kumasi.

Polluted water contains a variety of toxic compounds that tend to affect human health. Farmers have recently looked at runoff wastewater as a source of irrigation water because it comes at no cost and is a more efficient alternative to potable water due to the high demand but limited supply. This present study assesses the quality and suitability of water sources used for irrigation at the Kwame Nkrumah University of Science and Technology vegetable farmlands. The study specifically investigated the quality of water used for irrigation with the following parameters: pH, turbidity, total dissolved solids, total suspended solids, chloride, chemical oxygen demand, biological oxygen demand, oil and grease, fluoride, nitrate, nitrite, sulphate, sodium, calcium, magnesium, sodium adsorption potential, alkalinity, conductivity, phosphate, Escherichia coli, fecal and total coliforms. The results revealed that the water contained moderate levels of chloride and could be good for plant growth. The total coliform counts range from 2.1 × 106 to 4.15 × 107 MPN/100 mL, suggesting a relatively high microbial load in the irrigation water. The results also suggested that the sodium absorption ratio was very low and may not affect the quality of water for irrigation purposes. Fe levels far exceed the 5 mg/L maximum acceptable limits recommended by the WHO and FAO for the irrigation of vegetables. The high Fe concentration could discolor the leaves of some plants, especially foliage leaves. However, the levels of Cd were within the WHO maximum permissible limit of 0.01 mg/L.

An analysis of the foremost issues with artisanal and small-scale gold mining from Ghana's perspective.

Ghana has abundant mineral reserves in many of its regions, and gold mining remains one of the country's main sources of revenue. Given Ghana's current position in the global gold market, this review provides insight into the ASGM sector to give an understanding of the pertinent issues in the sector and its role in the socio-economic development of the country. This review assesses the effects of ASGM operations in economic, social, health, and environmental contexts to raise awareness of issues related to ASGM. It evaluates the measures taken to lessen the consequences of ASGM and maintain the sector's long-term viability. This review considers the foremost issues, including continued Hg use in ASGM, recent use of cyanide in ASGM, pollution of water bodies, and toxic metal contamination. It takes into account sustainable measures and remedial techniques that Ghana has implemented to alleviate the negative effects and support best mining practices. The primary factors influencing people to participate in ASGM are the need for quick sources of income, the scarcity of jobs in rural areas, the economic hardship, the need to supplement earnings from other activities like trading, and the comparatively meager profits from agricultural activities. Findings indicated that to gain more traction in addressing the challenges in the ASGM sector, the involvement of the community and direct stakeholders is essential to promoting responsible mining and environmentally sustainable practices. This review will increase awareness and pressure on decision-makers, researchers, and ASGM communities about the relevance of environmental conservation and sustainability.

Exposure and health risk assessment of transition metals in rice found on the Ghanaian market.

This study investigates the concentration of transition metals in imported and local rice brands on sale in some Ghanaian markets and the biochemical influences on the health of the Ghanaian population. A comparative analysis with previous studies in Ghana reveals that levels of Fe (3.64-4.44 mg/kg), Cd (0.03 mg/kg) and Cu (14.07-38.13 mg/kg) in the current study are much lower than the 13.67-21.35, 1.67-3.01, and 14.07-38.13 mg/kg recorded for Fe, Cd, and Cu, respectively. Rice sold in the Ghanaian markets contained different transition metals of which some are essential (Zn, Cu, Mn, and Fe). The levels of transition metals, such as Mn, Zn, Cd, Cu, and Fe are in moderate concentrations, which are well within the maximum acceptable limit of the World Health Organization. This study has revealed that R5 and R9 from the USA and India, respectively, recorded hazard indices above the safe limit of 1 and can therefore have the potential to pose detrimental health complications to consumers in the long term.

Multivariate studies and heavy metal pollution in soil from gold mining area.

Mining generates large volumes of waste, which if not regulated can release toxic metals, causing widespread environmental contamination. This study focused on heavy metal contamination in topsoil within a mining area at Nangodi in the Northern Region of Ghana. A total of 24 soil samples were collected from the study area and control samples were analyzed for Hg, Pb, Cd, As, Cr, and Fe using atomic absorption spectroscopy. Results of Pollution Index estimations and Geo-accumulation index (Igeo) classified the soil samples as moderately contaminated to heavily contaminated. Soil samples were severely enriched with As and moderately enriched with b, and Hg. Multivariate analyses such as factor analysis and cluster analysis were employed to examine the relationship between the metals and also differentiate the influence of the natural background content of metals from that due to human activities. Factor analysis identified three polluted soil factor associations. Cadmium, Fe, As, and Pb associated with factor 1, were due to anthropogenic activities. The high intercorrelation revealed by As and Pb shows similarity in their sources. Factor two dominated by Hg is considered an anthropogenic component. Factor 3 correlated with Cr and can be considered a natural component. Correlation analysis and cluster analysis supported each other. Results from the bi-plots showed that sites S1, S8, S11, and S18, have similar metal composition as the control site. Heavy metal contents in soils sampled from Zones A and B have been influenced by the mining activities as seen from the associations of these sites in the bi-plots. The results are useful for metal source identification, and can contribute to monitoring and regulatory programs.

Seasonal Changes in Pesticide Residues in Water and Sediments from River Tano, Ghana.

Pollution due to pesticide residues has been reported in the downstream of the Tano Basin in the rainy season and has been attributed to the anthropogenic activities upstream. However, data on the seasonal variations in pesticide residues in the upstream of Tano Basin are limited. Seasonal variations in 13 organochlorine pesticide residues, 8 organophosphorus pesticide residues, and 5 synthetic pesticide residues in water and sediment samples of River Tano upstream were assessed through extraction and Varian CP-3800 gas chromatography equipped with a CombiPAL Auto sampler set at ionization mode electron impact methods. Significantly higher pesticide residues were detected in water and sediment samples in the rainy season than the dry season. Permethrin (rainy: 0.007 ± 0.01 mg/kg; dry: 0.008 ± 0.02 mg/kg) and profenofos (rainy: 0.021 ± 0.02 mg/kg; dry: 0.026 ± 0.01 mg/kg) showed higher dry season concentrations in the sediment samples. Two isomers of lindane (δ-HCH = 0.059 ± 0.24 µg/L; γ-HCH = 0.002 ± 0.01 µg/L) were detected in the water in the rainy season, but 3 were detected in the sediment samples (δ-HCH = 0.004 ± 0.12 mg/kg; γ-HCH = 0.003 ± 0.01 mg/kg; aldrin = 0.001 ± 0.01 mg/kg) suggesting possible illegal use. The detected pesticide residual levels in both water and sediment samples were lower than the maximum residual levels in water and sediment. The Chemical Control and Management Centre of the Environmental Protection Agency should check possible faking and adulteration of banned organochlorine pesticides.

Boosting the photocatalytic H2 evolution activity of type-II g-GaN/Sc2CO2 van der Waals heterostructure using applied biaxial strain and external electric field.

Two-dimensional (2D) van der Waals (vdW) heterostructures are a new class of materials with highly tunable bandgap transition type, bandgap energy and band alignment. Herein, we have designed a novel 2D g-GaN/Sc2CO2 heterostructure as a potential solar-driven photocatalyst for the water splitting process and investigate its catalytic stability, interfacial interactions, and optical and electronic properties, as well as the effects of applying an electric field and biaxial strain using first-principles calculation. The calculated lattice mismatch and binding energy showed that g-GaN and Sc2CO2 are in contact and may form a stable vdW heterostructure. Ab initio molecular dynamics and phonon dispersion simulations show thermal and dynamic stability. g-GaN/Sc2CO2 has an indirect bandgap energy with appropriate type-II band alignment relative to the water redox potentials. Meanwhile, the interfacial charge transfer from g-GaN to Sc2CO2 can effectively separate electron-hole pairs. Moreover, a potential drop of 3.78 eV is observed across the interface, inducing a built-in electric field pointing from g-GaN to Sc2CO2. The heterostructure shows improved visible-light optical absorption compared to the isolated g-GaN and Sc2CO2 monolayers. Our study demonstrates that tunable electronic and structural properties can be realised in the g-GaN/Sc2CO2 heterostructure by varying the electric field and biaxial strain. In particular, the compressive strain and negative electric field are more effective for promoting hydrogen production performance. Since it is challenging to tune the electric field and biaxial strain experimentally, our research provides strategies to boost the performance of MXene-based heterojunction photocatalysts in solar harvesting and optoelectronic devices.

Assessment of ameliorative effects of organic dietary interventions on neonicotinoid exposure rates in a Japanese population.

Neonicotinoid insecticides (NNIs) are a popular class of insecticides used in various pest management regimens worldwide. Biomonitoring studies continuously report high exposure rates of NNIs in various human populations across the globe. Yet, there is no validated countermeasure for combating the recent exponential rise in NNI exposure rates observed in human populations. The current study assessed the impacts of organic dietary interventions on NNI exposure rates in a Japanese population. A total of 103 volunteers were recruited into the study. Subjects were either served with Organic diets for 5 and 30 days or conventional diets. A total of 919 repeated urine samples were collected from the participants and then subjected to LC-MS/MS analysis to determine urinary concentrations of 7 NNIs parent compounds and an NNI metabolite. Eight NNIs were detected; with a decreasing detection frequency (%Dfs) pattern; desmethyl-acetamiprid (dm-ACE) (64.96%) > dinotefuran (52.12%), imidacloprid (39.61%) > clothianidin (33.95%) > thiamethoxam (28.51%) > acetamiprid (12.62%) > nitenpyram (5.33%) > thiacloprid (2.83%). Dinotefuran, dm-ACE, and clothianidin recorded the highest concentrations in the subjects. The %Df of NNIs in the 5-days or 30-days organic diet group were lower than those of the conventional diet consumers. The organic diet group showed lower rates of multiple NNI exposures than those of the conventional diet consumers. The mean and median cumulative levels of NNIs (median IMIeq) were significantly lower in the organic diet group than the conventional diet group (p < 0.0001). The estimated daily intakes (EDIs) of NNIs were higher in adults than children, but less than 1% of NNI cRfDs, except for clothianidin, which exhibited a %cRfD of 1.32 in children. Compared to the conventional diet group, the 5- and 30-day organic dietary intervention showed drastic reductions in NNI EDIs. Findings from the present study give credence to organic dietary interventions as potential ameliorative strategies for NNI exposure rates in human populations.

Comprehensive evaluation of the possible impact of roofing materials on the quality of harvested rainwater for human consumption.

Water is a vital natural resource that influences life and contributes to domestic and industrial activities. Availability and accessibility of safe water sources are crucial for a country's economic growth. Harvesting of roof runoffs is an alternative source of water used for domestic purposes. However, the quality of harvested rainwater can be affected by roofing materials. This research seeks to assess heavy metals concentration, physicochemical and bacteriological parameters in roof runoffs and estimate the water quality index and health risk these metals pose to consumers. Rainwater samples were collected from Aluzinc, Aluminum, Galvanized, and Asbestos roofing materials within the Ejisu municipality. Rainwater collected directly from the sky was used as control. All runoffs from the four roofing materials recorded Cd, Fe, Cr, turbidity, pH, E.coli, total and fecal coliform levels above WHO limit for drinking water. There was a significant variation in pH, EC, Zn, Cd, Cr, Fe, E. coli, total and fecal coliform levels between control samples and roof runoffs (p < 0.05). The estimated water quality index for runoffs from the four roofing materials exceeded 100 indicating their unsuitability for drinking. Cadmium recorded the highest non-cancer health risk to children and adults in all the roof runoffs. Hazard quotients (HQs) above one were recorded for a child (HQ = 40.1) and an adult (HQ = 13.6) via dermal exposure to Cd in runoff from Aluminum roofing material. Hazard quotient of 9.53 and 4.08 were estimated for a child and an adult respectively via oral exposure to Cd in runoffs from Asbestos roofing material. The estimated cancer risks were above 10-3 for a child and an adult via oral and dermal exposure to Cd in all roof runoffs, suggesting a possible cancer health effect. The study concludes that the four roofing materials impact negatively on the quality of runoffs.

Neonicotinoid residues in commercial Japanese tea leaves produced by organic and conventional farming methods.

The current study sought to assess the residual levels of neonicotinoid insecticides (NEO) in organic and conventional green tea leaves produced in Japan. A total of 103 tea leaves (thus, 42 organic and 61 conventional), were sampled from grocery stores in Japan. Concentrations of NEOs in the tea leaves were quantified using LC-MS/MS; and the data was used to estimate maximum daily intakes of NEOs within the Japanese population. Seven native NEO compounds and one NEO metabolite were detected in both organic and conventional tea leaves. Detection frequencies (%Dfs) of NEOs in the tea samples (n = 103) were found in the decreasing order; thiacloprid (84.47 %) > dinotefuran (74.76 %) > imidacloprid (69.90 %) ≈ clothianidin (69.90 %) > dm-acetamiprid (63.11 %) > thiamethoxam (58.25 %) > acetamiprid (4.85 %) > nitenpyram (1.94 %). About 94.20 % of the tea leaves contained two or more NEO compounds simultaneously. The %Dfs of NEOs were relatively lower in organic tea leaves, compared to the conventional tea leaves. Various percentile concentrations of NEOs were far lower in organic tea leaves, compared to the conventional tea leaves. The maximum daily intakes of NEOs through consumption of tea (MDIgt) were also lower for organic tea leaves, compared to the conventional tea samples.

Simultaneous quantification of imidacloprid and its metabolites in tissues of mice upon chronic low-dose administration of imidacloprid.

This study aimed to (i) develop a sensitive method for simultaneous detection and quantification of imidacloprid (IMI) and seven of its metabolites in tissue specimens, and to (ii) determine the biodistribution of the IMI compounds in tissues of C57BL/6J male mice; after exposure to 0.6 mg/kg bw/day of IMI (10% of no observable adverse effect level of IMI) through a powdered diet for 24 weeks. We successfully developed a method which was accurate (recoveries were ≥ 70% for most compounds), sensitive (LODs ≤ 0.47 ng/mL and LOQs ≤ 1.43 ng/mL were recorded for all detected compounds, R2 ≥ 0.99) and precise (RSDs ≤ 20%) for routine analysis of IMI and seven of its metabolites in blood and various tissue matrices. After bio-distributional analysis, IMI and five of its metabolites were detected in mice. Brain, testis, lung, kidney, inguinal white adipose tissue and gonadal white adipose tissue mainly accumulated IMI, blood and mesenteric white adipose tissue mainly accumulated IMI-olefin; liver mainly accumulated desnitro-IMI; pancreas predominately accumulated 4-hydroxy-IMI. The desnitro-dehydro-IMI and the desnitro-IMI metabolites recorded tissue-blood concentration ratios ≥ 1.0 for testis, brain, lung and kidney. The cumulative levels of the six detected IMI compounds (Σ6 IMI compounds) were found in the decreasing order: blood > testis > brain > kidney > lung > iWAT > gWAT > mWAT > liver > pancreas. Altogether, this study provided essential data needed for effective mechanistic elucidation of compound-specific adverse outcomes associated with chronic exposures to IMI in mammalian species.

Human Exposures to Neonicotinoids in Kumasi, Ghana.

Neonicotinoid insecticides (NNIs) are now popular in many agricultural systems across Africa; however, the extent of human exposures to NNIs in African countries is scarcely reported. The present study evaluates neonicotinoid exposures in the consumer population of Kumasi, a cosmopolitan city in Ghana. A total of 75 human urine samples were collected from healthy volunteers (nonfarmers, aged 13-80 yr) and analyzed with a liquid chromatography electrospray ionization tandem mass spectrometry system. Seven NNIs and 3 NNI metabolites were detected in the following pattern (frequency, median concentration, maximum concentration): N-dm-acetamiprid (94.7%, 0.41 µg/L, 8.79 µg/L) > imidacloprid (70.7%, 0.15 µg/L, 211.62 µg/L) > N-(6-chloro-3-pyridylmethyl)-N-ethyl-N'-methylformamidine (62.2%, 0.43 µg/L, 53.85 µg/L) > 2-[N-(6-chloro-3-pyridylmethyl)-N-ethylamino]-2-(methylimino)acetic acid (56.8%, 0.10 µg/L, 3.53 µg/L) > clothianidin (40%, >limit of quantification [LOQ], 0.45 µg/L) > nitenpyram (18.7%, >LOQ, 0.14 µg/L) ≈ thiamethoxam (18.7%, >LOQ, 0.21 µg/L) > dinotefuran (12.0%, >LOQ, 1.01 µg/L) > acetamiprid (2.7%, >LOQ, 0.08 µg/L) ≈ thiacloprid (2.7%, >LOQ, 0.14 µg/L). Approximately 92% of the subjects were found to be exposed to multiple neonicotinoids simultaneously. The mean, median, and maximum imidacloprid equivalent of the relative potency factor of NNIs were found to be 1.6, 0.5, and 22.52, respectively. The median estimated daily intakes of acetamiprid, imidacloprid, and nitenpyram were 0.47, 1.27, and 0.02 µg/kg/d for females and 0.91, 0.66, and 0.08 µg/kg/d for males, respectively. The maximum daily intakes of all the NNIs were <1% of their chronic reference doses (cRfDs), except for imidacloprid and thiacloprid which recorded maximum daily intakes corresponding to 17.97 and 8.28% of cRfDs, respectively. To the best of our knowledge, the present study is the first biomonitoring report on neonicotinoid insecticides in Africa. Environ Toxicol Chem 2021;40:2306-2318. © 2021 SETAC.

Developmental neurotoxicity of low concentrations of bisphenol A and S exposure in zebrafish.

The vulnerability to environmental insults is heightened at early stages of development. However, the neurotoxic potential of bisphenol A (BPA) and bisphenol S (BPS) at developmental windows remains unclear. To investigate the mechanisms mediating the developmental neurotoxicity, zebrafish embryos were treated with 0.01, 0.03, 0.01, 0.3, 1 µM BPA/BPS. Also, we used Tg(HuC:GFP) zebrafish to investigate whether BPA/BPS could induce neuron development. The reduction in body length, and increased heart rate were significant in 0.3 and 1 µM BPA/BPS groups. The green fluorescence protein (GFP) intensity increased at 72 hpf and 120 hpf in Tg(HuC:GFP) larvae which was consistent with the increased mRNA expression of elval3 following BPS treatments, an indication of the plausible effect of BPS on embryonic neuron development. Additionally, BPA/BPS treatments elicited hyperactivity and reduced static time in zebrafish larvae, suggesting behavioral alterations. Moreover, qRT-PCR results showed that BPA and BPS could interfere with the normal expression of development-related genes vegfa, wnt8a, and mstn1 at the developmental stages. The expression of neurodevelopment-related genes (ngn1, elavl3, gfap, α1-tubulin, mbp, and gap43) were significantly upregulated in BPA and BPS treatments, except for the remarkable downregulation of mbp and gfap elicited by BPA at 48 (0.03 µM) and 120 hpf (0.3 µM) respectively; ngn1 at 48 hpf for 0.1 µM BPS. Overall, our results highlighted that embryonic exposure to low concentrations of BPA/BPS could be deleterious to the central nervous system development and elicit behavioral abnormalities in zebrafish at developmental stages.

Characterization, Spatial Variation and Risk Assessment of Heavy Metals and a Metalloid in Surface Soils in Obuasi, Ghana.

BACKGROUND: Soil contamination with heavy metals and metalloids has become an increasingly important issue in recent years. OBJECTIVES: The present study examines possible contamination of the environment with metals from gold mining activities in Obuasi, Ghana. METHODS: Soil samples were collected from commercial and residential areas and tailing dams in Obuasi in order to investigate the extent of lead (Pb), cadmium (Cd), mercury (Hg), copper (Cu), zinc (Zn), cobalt (Co), chromium (Cr), nickel (Ni) and arsenic (As) pollution, create thematic maps showing the extent of heavy metals pollution, identify the sources of pollution, and to assess risks to humans and the surrounding ecological system. RESULTS: Mean concentrations of metals from the study were found in the order of As > Cu > Zn > Cr > Ni > Pb > Co > Hg > Cd. The results showed that all communities were severely polluted with As, and distribution maps highlighted two hot spots at the sulfite treatment plant and Pompura treatment plant tailings dams. Additionally, the levels of Pb, Cu and Zn were elevated around the city center where vehicular traffic is very dense. Principal component analysis indicated that mining activities may have significantly contributed to metal levels in Obuasi soils. The potential ecological risk (RI) indicated that soils in 41% of the communities pose very high risks to the surrounding ecological system, 50% pose considerable risk, and 9% pose a moderate risk. Arsenic and Hg contributed 73 and 15% of the RI, respectively. The average hazard quotient due to soil As exposure was 2.51 ± 1.23 and ingestion of soils in 95% of the communities in the study area could pose non-carcinogenic health risks to children. Moreover, the average cancer risk for children from the communities was 1.13 × 10-3. Based on the United States Environmental Protection Agency (USEPA) recommendation for cancer risk of 10-6 to 10-4, the cancer risk for children (> 10-3) was higher in 45% of the studied communities. CONCLUSIONS: The central part of the study area is polluted with Pb, Zn and Cu, and As pollution is severe in all of the studied communities. The RI from all study sites revealed very high risk to the ecological system, including mammals. There could be non-cancer and cancer risks to Obuasi residents due to ingestion of As-contaminated soils, and children are particularly vulnerable. COMPETING INTERESTS: The author declares no competing financial interests.

Bioaccumulation factors and multivariate analysis of heavy metals of three edible fish species from the Barekese reservoir in Kumasi, Ghana.

The Barekese reservoir has seen persistent degradation for decades through anthropogenic activities within its catchment. In spite of this, heavy metal pollution profile of fish species of the reservoir is not well studied. Total concentrations of Hg, Cd, Ni, and Cr were analyzed in muscle tissues of 45 edible fish species comprising (Tilapia zilli, Oreochromis niloticus, and Heterotis niloticus) from the reservoir. The examined fish species showed varying bioaccumulative factors for detected metals. A PCA biplot revealed that Cd showed association with all examined fish species used in the study indicating that Cd in the reservoir is biologically available for uptake by all examined fish species. A dendrogram of cluster analysis also revealed that Cd and Cr are in one cluster indicating their associations and similarities to a common anthropogenic source. The study suggests that anthropogenic activities are possible sources of heavy metal pollution in the Barekese reservoir and, hence, the examined fish species could be used to monitor the levels of Ni, Cr, Hg, and Cd in the reservoir.

Sex and site differences in urinary excretion of conjugated pyrene metabolites in the West African Shorthorn cattle.

Industrialization, economic and population growth rates in Ghana have increased the release of contaminants including polycyclic aromatic hydrocarbons (PAHs) into the environment through which humans and animals are exposed. Cattle is reported to be exposed to high levels of PAHs through feed and inhalation. Once exposed, PAHs are metabolized and excreted in urine, feces or bile. In a previous study, cattle in Ghana was reported to excrete high levels of 1-hydroxypyrene (1-OHPyr) due to high exposure to the parent compound, pyrene. 1-OHPyr is further metabolized to glucuronide and sulfate conjugates. Thus, the aim of this study was to investigate the sex and site differences in urinary excretion of conjugated pyrene metabolites using cattle urine collected from rural and urban sites of the Ashanti region, Ghana. From the results, geometric mean concentration adjusted by specific gravity indicated that 1-OHPyreneGlucuronide (PyG) was the most abundant conjugate followed by PyrenediolSulfate (M3). The sum of conjugated pyrene metabolites and sum of both conjugated and deconjugated pyrene metabolites correlated significantly with PyG, PydiolSulfate (M2) and PydiolSulfate (M3). The study revealed no significant difference in urinary excretion of conjugated pyrene metabolites between rural and urban sites. This indicated that similar to urban sites, cattle in rural sites were exposed to high levels of pyrene. There was no significant difference in urinary concentrations of conjugated pyrene metabolites between sexes.

Association between human exposure to heavy metals/metalloid and occurrences of respiratory diseases, lipid peroxidation and DNA damage in Kumasi, Ghana.

Heavy metals and metalloids contamination in soils, water, food and livers of wild rats have been studied in Kumasi, Ghana and despite the estimated risks to residents, there is no epidemiological study to ascertain these projections. In addition, the World Health Organization and International Agency for Research on Cancer have reported an increase in respiratory diseases and cancers, in Ghana. The study's purpose was therefore to explore the potential associations between metal exposure and occurrences of respiratory diseases, lipid peroxidation and/or DNA damage to different age groups and sexes in Kumasi. Human urine was collected from the general population in urban and control sites in Kumasi and nine metals were measured in each sample. Results showed that although Zn was the most abundant total urinary As concentration was higher in 83% of samples compared to reference values. Urinary concentrations of metals, malondialdehyde (MDA) and 8-hydroxy-2-deoxy-guanosine (8-OHdG) were higher in urban sites compared to the control site. Based on the results obtained, there was no significant correlation between urinary metals and age. However, urinary Cd and MDA were highest in age groups 61-85 and 3-20 years, respectively. Significantly higher levels of urinary Co, As and Cd were detected in female participants. The study revealed that exposure to As was significantly associated with increased odds of asthma (odds ratio (OR) = 2.76; CI: 1.11-6.83) and tachycardia (OR = 3.93; CI: 1.01-15.4). Significant association was observed between urinary metals and MDA and 8-OHdG indicating possibility of lipid peroxidation and/or DNA damage in Kumasi residents.

Oxidative stress and respiratory symptoms due to human exposure to polycyclic aromatic hydrocarbons (PAHs) in Kumasi, Ghana.

Studies of polycyclic aromatic hydrocarbons (PAHs) and its metabolites in PM10, soils, rat livers and cattle urine in Kumasi, Ghana, revealed high concentrations and cancer potency. In addition, WHO and IARC have reported an increase in cancer incidence and respiratory diseases in Ghana. Human urine were therefore collected from urban and control sites to: assess the health effects associated with PAHs exposure using malondialdehyde (MDA) and 8-hydroxy-2-deoxyguanosine (8-OHdG); identify any association between OH-PAHs, MDA, 8-OHdG with age and sex; and determine the relationship between PAHs exposure and occurrence of respiratory diseases. From the results, urinary concentrations of the sum of OH-PAHs (∑OHPAHs) were significantly higher from urban sites compared to the control site. Geometric mean concentrations adjusted by specific gravity, GMSG, indicated 2-OHNaphthalene (2-OHNap) (6.01 ± 4.21 ng/mL) as the most abundant OH-PAH, and exposure could be through the use of naphthalene-containing-mothballs in drinking water purification, insect repellent, freshener in clothes and/or "treatment of various ailments". The study revealed that exposure to naphthalene significantly increases the occurrence of persistent cough (OR = 2.68, CI: 1.43-5.05), persistent headache (OR = 1.82, CI: 1.02-3.26), tachycardia (OR = 3.36, CI: 1.39-8.10) and dyspnea (OR = 3.07, CI: 1.27-7.43) in Kumasi residents. Highest level of urinary 2-OHNap (224 ng/mL) was detected in a female, who reported symptoms of persistent cough, headache, tachycardia, nasal congestion and inflammation, all of which are symptoms of naphthalene exposure according to USEPA. The ∑OHPAHs, 2-OHNap, 2-3-OHFluorenes, and -OHPhenanthrenes showed a significantly positive correlation with MDA and 4-OHPhenanthrene with 8-OHdG, indicating possible lipid peroxidation/cell damage or degenerative disease in some participants. MDA and 8-OHdG were highest in age group 21-60. The present study showed a significant sex difference with higher levels of urinary OH-PAHs in females than males.

Contamination Levels and Sources of Heavy Metals and a Metalloid in Surface Soils in the Kumasi Metropolis, Ghana.

BACKGROUND: Environmental contamination with heavy metals and metalloids due to industrial, smelting and mining activities have become common in large and growing cities. Kumasi is one of the most industrialized cities in Ghana and experiences metal pollution due to recent and past activities. Although metals are naturally abundant in the area, their accumulation in soils could potentially lead to adverse effects on local ecosystems. OBJECTIVES: The aims of this study were to determine the distribution, enrichment, geoaccumulation and sources of metals in Kumasi soils and to estimate the contamination factor (CF) and pollution load index (PLI) of these metals in soils. METHODS: Concentrations of eight heavy metals and a metalloid were determined in 112 soil samples randomly collected from 31 sampling sites in the area. In addition, 5 soil samples were collected from a pristine site (Kwame Nkrumah University of Science and Technology Botanical Gardens) for data comparison, to determine the local background values for metal concentrations and to evaluate the extent of metal pollution in the study area. RESULTS: Heavy metals such as zinc (Zn), lead (Pb), cadmium (Cd) and chromium (Cr) were enriched in 65, 32, 58 and 93% of the sampling sites, respectively, and geo-accumulation indexes for Cr, Zn, Cd, mercury (Hg) and Pb showed moderate to extreme contamination in 100, 97, 77, 65 and 45% of the sampling sites, respectively. Principal component and cluster analyses revealed that industrial activities including mining were the major sources of metals in Kumasi soils with high metal input in the community of Suame. Distribution maps revealed hotspots of Cd, nickel (Ni), arsenic (As), cobalt (Co), copper (Cu) and Pb in Suame. The highest CFs for Cu, Cd, Ni, As, Co and Pb highlighted anthropogenic inputs in Suame, while Hg was highest in Mbrom, Zn in Suntreso, and Cr in Aboabo. CONCLUSIONS: The PLI of metals revealed Suame as the most polluted study site, while Anomangye and Bomso were the least polluted.

Pesticide residues in water, sediment and fish from Tono Reservoir and their health risk implications.

Levels of organochlorine (OC) and organophosphorus (OP) pesticide residues in fish, sediments and water and their health risk associated with the consumption of the fish from the Tono Reservoir, Ghana were evaluated. The analytical methods included solvent extraction of the pesticide residues using ultrasound sonication and soxhlet extraction and their subsequent quantification using GC equipped with electron capture detector and pulse flame photometric detector after clean-up on activated silica gel/anhydrous sodium sulphate. A total of 29 pesticides comprising 16 OCs and 13 OPs were analyzed, out of which aldrin, p,p'-DDE and p,p'-DDD were detected in fish and sediment samples. The results showed that all the residues in water had their concentrations below the detection limit. Mean concentrations of organochlorine pesticide (OCP) residues in fish ranged from 0.017 to 0.17, 0.043 to 0.30, 0.027 to 0.243 and 0.097 to 0.263 µg/g in Sarotherodon galilaeus, Clarias anguillaris, Schilbe intermedius and Marcusenius senegalensis respectively. Mean concentrations of organophosphates pesticides ranged from 0.080 to 0.090, 0.080 to 0.087 and 0.050 to 0.063 µg/g in C. anguillaris, S. intermedius and M. senegalensis respectively. The level of chlorpyrifos in S. galilaeus was 0.160 µg/g. Mean concentrations of OCP residue in sediments ranged from 0.047 to 0.090 µg/g. Aldrin recorded the highest level while p,p'-DDD recorded the lowest level. The mean concentrations for all the detected residues were below the WHO/FAO maximum residue limits. Health risk estimation revealed that aldrin in M. senegalensis had great potential for systemic toxicity to consumers.

Excretion of polycyclic aromatic hydrocarbon metabolites (OH-PAHs) in cattle urine in Ghana.

Previous studies of polycyclic aromatic hydrocarbons (PAHs) in particulate matter, soils and livers of wild rats indicated that the city centre of Kumasi, Ghana has been severely polluted with high cancer potency. Cattle urine were therefore collected from Kumasi (urban) and Offinso (rural), Ghana: to determine concentrations of urinary PAH metabolites (OH-PAHs); and find their association with sex; and to estimate exposure of cattle to PAHs from the different sites. From the results, geometric mean concentrations (adjusted by specific gravity), GMSG, showed that 2-OHNaphthalene (2-OHNap) was the most abundant OH-PAH in cattle urine from all study sites, and naphthalene-containing-mothballs might have contributed significantly to the levels. There was no significant difference between urinary OH-PAHs concentrations in cattle from urban and rural sites except for 2-OHPhe and 4-OHPhe, and similar to urban areas, rural sites could also be polluted with PAHs. GMSG of 2-OHNap in cattle urine in Kokote (21.9 ± 6.51 ng/mL; a rural area), was significantly higher compared to the other sites followed by Oforikrom (4.15 ± 4.37 ng/mL; urban). The GMSG concentration (ng/mL) of the sum of OH-PAHs decreased in the order, Kokote (44.7) > Oforikrom (7.87) > Saboa (6.98) > Santasi (6.68) > and Twumasen Estate (5.23). The high concentrations of urinary 2-OHNap, 2-3-OHFlu, 2-OHPhe, 3-OHPhe and 4-OHPhe in Kokote indicated high PAHs exposure to cattle in this area or different/specific source of PAHs exposure. GMSG of 2-OHNap was significantly higher in male cattle compared to females while 1-9-OHPhe was significantly higher in females.