[Lupus erythematosus: effects of different therapeutic methods on intranuclear characteristics of lymphoid cells]. / Vliianie razlichnykh metodov lecheniia krasnoi volchanki na vnutriiadernye kharakteristiki limfoidnykh kletok.

In lymphocytes of peripheral blood from 46 patients with various forms of lupus erythematosus measurements were made of histone fractions and DNA. It is proved that intranuclear characteristics of lymphoid cells can be used in the choice of treatment and assessment of its efficacy. Lupus erythematosus treatment with glucocorticoids (diprospan, diprospan + celeston) is effective in correction of intranuclear lymphocyte changes in this disease.

[An analysis of the lymphoid cell histones in patients with different forms of lupus erythematosus]. / Analiz gistonov limfoidnykh kletok u bol'nykh s razlichnymi formami krasnoi volchanki.

The study was made of lisine- and arginine-rich histone fractions and DNA in lymphocytes of peripheral blood in patients with lupus erythematosus. The findings showed intranuclear changes of immunocompetent cells in various forms of the disease. The above parameters may be used in the diagnosis, evaluation of treatment efficacy, prognosis.

[The chemical-toxicological analysis of ergot alkaloids]. / Khimiko-toksikologicheskii analiz alkaloidov sporyn'i.

Russian and foreign literature dealing with this topic is reviewed. Brief descriptions of methods of examinations starting from color tests and up to high-pressure liquid chromatography and mass spectrometry are presented in a chronological order. Clinical and toxicological characteristics of some ergot alkaloids are described.

[Effect of T-activin on peripheral immunity organs in intact and thymectomized mice]. / Vliianie T-aktivina na perifericheskie organy immuniteta intaktnykh i timéktomirovannykh myshei.

Morphometry of the spleen, axillary lymph nodes and cytological assay of the bone marrow and peripheral blood were performed in (CBA X C57BL)F1 mice 1, 5, 10 and 15 days after subcutaneous injection of 0.5 microgram T-activin to intact and thymectomized (when adult) mice 2 months after operation. It was demonstrated that in intact animals, injection of T-activin stimulated the whole system of immunogenesis. The time course of plasmatization and the response of the germinative centers differing from that seen during antigen administration suggests that T-activin is not immunogenous, acting as a stimulant of the previous immune responses. The permanent amount of the degenerating cells attests to the lack of the toxic drug effect.

[Effect of T-activin on thymocyte reproduction and migration]. / Vliianie T-aktivina na reproduktsiiu i migratsiiu timotsitov.

Forty seven F1 (CBA X C57Bl) mice were used for quantitative morphologic examination of the thymus on 1, 5, 10 and 15 days after a single injection of 0.5 microgram T-activin, and injections of 0.1 microgram of T-activin once a day during 5 days. The number of transformed thymocytes and mitoses figures in cortex was found to be increased reaching a maximum at the 5th day as regards the magnitude and spreading. By the end of the research the number of transformed thymocytes and mitoses returned to initial values. There was a periodical (at the 5th and 10th days) 1 mm2 reduction in the number of thymocytes, an increase in the proportion of medullary thymocytes, reaching maximum at the 5th day, and a tendency towards the reduction of a relative area of the parenchyma. These indicators did not return to the initial values by the 15th days. However there was a tendency towards normalization. The conclusion is made about the stimulatory effect of T-activin on reproduction and migration of thymocytes.

Factors controlling stem cell recirculation. III. Effect of the thymus on the migration and differentiation of hemopoietic stem cells.

Experiments were carried out to investigate the effect of thymectomy on the migration and differentiation of hemopoietic stem cells released from shielded parts of bone marrow after irradiation of mice with lethal doses of x-rays. In 2-3 wk after thymectomy, the rate of migration declined and the differentiation of stem cells into granulocytic colonies was inhibited. Transplantation of syngeneic thymus or lymph node cells into thymectomized mice enhanced the migration of stem cells from the bone marrow and restored the usual pathways of their differentiation.