The impact of simulated 3x3 tournament on vertical jump force-time metrics in national team male basketball players.

With innovative portable force plate systems being widely implemented for lower-body neuromuscular performance assessment in an applied sports setting and the existing gap in the scientific literature regarding player performance during in-game competitive scenarios, the purpose of the present study was to compare changes in countermovement vertical jump (CVJ) performance pre-post a simulated 3×3 basketball tournament. Seven current or former members of a 3×3 national basketball team volunteered to participate in the present investigation. Upon completing standardized warm-up procedures, athletes stepped on a uni-axial force plate system sampling at 1,000 Hz and performed three maximal-effort CVJs with no arm swing. Then, the athletes proceeded to play a simulated 3×3 basketball tournament composed of two consecutive games, separated by a 15-min rest interval. Immediately following the completion of the second game, the identical CVJ testing procedures were repeated. Paired sample t-tests were used to examine pre-post-tournament differences in nineteen CVJ performance metrics (p < 0.05). The results reveal that force-time metrics during both eccentric and concentric phases of the CVJ remain relatively unchanged pre-post simulated 3×3 basketball tournament. However, multiple force-time metrics within the eccentric phase of the CVJ changed by 12.1%-19.1% (e.g., eccentric peak power and peak velocity, eccentric duration), suggesting that the eccentric phase of CVJ might be responsive to performance stimulus to a greater extent than the concentric phase. Overall, these findings further support the importance of comprehensive CVJ analysis when intending to measure changes in neuromuscular performance.

Effects of mining activities and municipal wastewaters on element accumulation and integrated biomarker responses of the European chub (Squalius cephalus).

This study aimed to determine concentrations of 29 elements in the gills and liver as well as biomarker response in gills, liver, and blood of European chub from Pek River (exposed to long-term mining activities), and to compare these findings with individuals from Ibar River (influenced by emission of treated municipal wastewater) and Kruscica reservoir (source of drinking water) using inductively-coupled plasma optical emission spectrometry (ICP-OES). The metal pollution index (MPI) was also calculated. Supporting analyses for the detection of the municipal wastewater presence at investigated localities included analyses of microbiological indicators (total coliforms and Escherichia coli) of faecal pollution. We have assessed biomarker responses from molecular to organism level using the condition index, comet assay, micronucleus test, oxidative stress parameters, histopathological alterations, and fluorescence spectroscopy parameters. Multibiomarker analysis was summarized by Integrated Biomarker Response v2 (IBRv2). Among these locations, Kruscica exhibited the lowest, whereas the Pek River displayed the highest values for both categories of indicator bacteria. Due to the porphyry copper ores mining, individuals from Pek River had several times higher Cu concentrations in both gills and liver compared to the other localities which was confirmed by biomarker responses and IBRv2 value. On the contrary, fish from Kruscica reservoir were the least affected by elemental pollution which is also confirmed by low MPI and IBRv2 values. Responses of biomarkers correspond to the elemental accumulation in the liver and gills of the Ibar River are positioned between the Pek River and Kruscica reservoir. Of all the biomarkers analyzed in this study, the condition index was the least sensitive. The results of this study showed that fluorescence spectroscopy may be a method for fast screening of structural changes in gills caused by the pollution burden.

Does the short-term learning effect impact vertical jump performance assessment on a portable force plate system?

One of the reoccurring questions that arises during the countermovement vertical jump (CVJ) assessment is whether the learning effect has an impact on the accuracy of the results obtained. Thus, the purpose of the present investigation was to examine the impact of the short-term learning effect on the assessment of lower-body neuromuscular performance characteristics when performed on a portable one-dimensional force plate system. Sixteen recreationally active college-age males volunteered to participate in the present study. Each participant completed four sets of three non-consecutive CVJs with no arm swing throughout a single day. Besides strong verbal encouragement, participants were constantly instructed to focus on pushing the ground as explosively as possible. Fourteen force-time metrics were selected for CVJ performance analysis purposes: eccentric and concentric peak and mean force and power, eccentric and concentric duration, contraction time, jump height, reactive strength index-modified, and countermovement depth. Repeated measures multivariate analysis of variance was used to examine statistically significant differences across four testing time points (p < 0.05). The results indicate an absence of any meaningful differences across four testing time points in force-time metrics of interest during both eccentric and concentric phases of the CVJ. Moreover, no differences were observed in CVJ outcome metrics such as countermovement depth, suggesting that the movement strategy tends to remain consistent. Overall, these findings reveal that CVJ test repeatability is not affected by the short-term learning effect and that data are stable at least within the scope of this study and within this population.

Relationship between vertical jump performance and playing time and efficiency in professional male basketball players.

With innovative force plate technology being available to many sports organizations worldwide that allow for time-efficient in-depth neuromuscular performance assessment, the purpose of the present study was to examine the relationship between some of the most commonly analyzed countermovement vertical jump (CVJ) force-time metrics and basketball playing time and efficiency. Twenty-four professional male basketball players volunteered to participate in the present study. The CVJ testing procedures were conducted within the first quarter of the competitive season span. Following a standardized warm-up protocol, each athlete stepped on a dual uni-axial force plate system sampling at 1,000 Hz and performed three maximum-effort CVJs with no arm swing. To minimize the possible influence of fatigue, each jump trial was separated by a 10-15 s rest interval and the average value across three jumps was used for performance analysis purposes. Basketball playing efficiency and average playing time were obtained at the end of the regular season competitive period from the coaching staff records and the official team records. Pearson product-moment correlation coefficients (r) were used to examine the strength of the relationships between force-time metrics and basketball playing time and efficiency, separately for each dependent variable (p < 0.05). A significant positive association was observed between playing efficiency and eccentric mean force and eccentric mean and peak power (r = 0.406-0.552). Similarly, an increase in eccentric mean power was positively correlated with the number of minutes played during the competitive season (r = 0.464). Moreover, the aforementioned relationship remained present even when eccentric mean power was expressed relative to the player's body mass (r = 0.406). Thus, the findings of the present study indicate that, at the professional level of men's basketball competition, CVJ eccentric strength and power have a positive impact on both playing time and efficiency.

Exploring the Antibacterial Potential of Lamiaceae Plant Extracts: Inhibition of Bacterial Growth, Adhesion, Invasion, and Biofilm Formation and Degradation in Pseudomonas aeruginosa PAO1.

In response to the global rise in antibiotic resistance and the prevalence of bacterial biofilm-related infections, the antibacterial efficacy of methanolic, ethanolic, and aqueous extracts of 18 Lamiaceae plants from Serbia was evaluated. The total coumarins and triterpenes were detected spectrophotometrically, while a microdilution assay measured their effects on bacterial growth. Additionally, the impact of these extracts was assessed on Pseudomonas aeruginosa PAO1 adhesion and invasion in human fibroblasts and biofilm formation and degradation. The alcoholic extracts had the highest phytochemical content, with Teucrium montanum and Lavandula angustifolia being the richest in coumarins and triterpenes, respectively. Gram-positive bacteria, particularly Bacillus subtilis, were more susceptible to the extracts. Hyssopus officinalis ethanolic and Sideritis scardica methanolic extracts inhibited bacterial growth the most efficiently. Although the extracts did not inhibit bacterial adhesion, most ethanolic extracts significantly reduced bacterial invasion. Origanum vulgare and H. officinalis ethanolic extracts significantly inhibited biofilm formation, while Teucrium chamaedrys extract was the most active in biofilm degradation. This study significantly contributes to the literature by examining the antibacterial activity of Lamiaceae extracts, addressing major literature gaps, and underscoring their antibacterial potential, particularly Satureja montana and O. vulgare ethanolic extracts, linking their efficacy to coumarins and triterpenes.

Influence of alkylthio and arylthio derivatives of tert-butylquinone on the induction of DNA damage in a human hepatocellular carcinoma cell line (HepG2).

The aim of this study was to investigate the effects of tert-butylquinone (TBQ) and its alkylthio and arylthio derivatives on DNA in vitro, using acellular and cellular test systems. Direct interaction with DNA was studied using the plasmid pUC19. Cytotoxic (MTS assay) and genotoxic (comet assay and γH2AX focus assays) effects, and their influence on the cell cycle were studied in the HepG2 cell line. Our results show that TBQ and its derivatives did not directly interact with DNA. The strongest cytotoxic effect on the HepG2 cells was observed for the derivative 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone (IC50 64.68 and 55.64 µM at 24-h and 48-h treatment, respectively). The tested derivatives did not significantly influence the cell cycle distribution in the exposed cellular populations. However, all derivatives showed a genotoxic activity stronger than that of TBQ in the comet assay, with 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone producing the strongest effect. The same derivative also induced DNA double-strand breaks in the γH2AX focus assay.

Vertical jump neuromuscular performance of professional female handball players-starters vs. non-starters comparison.

Given the complex nature of the handball as a game, players are required to possess a distinct set of physical and physiological attributes to attain peak performance. With the countermovement vertical jump (CVJ) being widely implemented as a non-invasive and time-efficient testing modality in sports settings, the purpose of the present study was twofold: (a) to establish a CVJ profile of professional female handball players and (b) to examine differences in force-time metrics between starters and non-starters. Forty-two professional female handball players (e.g., SuperLeague) volunteered to participate in this study. Each athlete performed three maximum-effort CVJs with no arm swing while standing on a uni-axial force plate system sampling at 1,000 Hz. Independent t-tests were used to examine differences in each variable between starters and non-starters. The results revealed that starters attained superior performance within the eccentric phase of the CVJ when compared to non-starters, particularly in terms of eccentric peak velocity (-0.957 ± 0.242 vs. -0.794 ± 0.177 m·s-1), eccentric mean power (320.0 ± 77.7 vs. 267.1 ± 75.2 W), and eccentric peak power (929.0 ± 388.1 vs. 684.4 ± 214.2 W). While not reaching the level of statistical significance, moderate-to-large effect sizes were observed for concentric impulse, peak velocity, and mean and peak force and power, all in favor of players included in the starting lineup (g = 0.439-0.655). Overall, these findings suggest that at the top-tier level of handball competition, the ability to secure a spot in a starting lineup may be possibly influenced by the athlete's eccentric performance capabilities. Thus, the development of lower-body eccentric strength and power may positively impact on-court athlete performance and ultimately help the team secure the desired game outcome.

Comparison of vertical jump and sprint performances between 3 × 3 and 5 × 5 elite professional male basketball players.

Given its fast-growing popularity and unique on-court competitive demands, 3 × 3 basketball has captured a considerable amount of attention over recent years. However, unlike research focused on studying 5 × 5 basketball players, there is a lack of scientific literature focused on examining countermovement vertical jump (CMJ) and sprint performance characteristics of 3 × 3 athletes. Thus, the purpose of the present study was to compare force-time metrics during both eccentric and concentric phases of the CMJ and acceleration and deceleration capabilities between 3 × 3 and 5 × 5 top-tier professional male basketball athletes. Ten 3 × 3 and eleven 5 × 5 professional basketball players volunteered to participate in the present study. Upon completion of a standardized warm-up, each athlete performed three maximum-effort CMJs, followed by two 10 m sprints. A uni-axial force plate system sampling at 1,000 Hz was used to analyze CMJ force-time metrics and a radar gun sampling at 47 Hz was used to derive sprint acceleration-deceleration measures. Independent t-tests and Hedge's g were used to examine between-group statistically significant differences (p < 0.05) and effect size magnitudes. The findings of the present study reveal that 3 × 3 and 5 × 5 professional male basketball players tend to display similar neuromuscular performance characteristics as no significant differences were observed in any force-time metric during both eccentric and concentric phases of the CMJ (g = 0.061-0.468). Yet, prominent differences were found in multiple measures of sprint performance, with large effect size magnitudes (g = 1.221-1.881). Specifically, 5 × 5 basketball players displayed greater average and maximal deceleration and faster time-to-stop than their 3 × 3 counterparts. Overall, these findings provide reference values that sports practitioners can use when assessing athletes' CMJ and sprint performance capabilities as well as when developing sport-specific training regimens to mimic on-court competitive demands.

Different Body Partialization Procedures Considering Maximum Strength and Explosiveness: Factorial Analysis Approach / Diferentes Procedimientos de Parcialización Corporal Considerando Fuerza Máxima y Explosividad: Enfoque de Análisis Factorial

SUMMARY: The aim of this study was to determine the sensitivity of different methods of partialization, in terms of different body component indices in relation to indicators of strength and explosiveness. The research involved 187 subjects who were divided into two groups based on sex. This research consisted of measuring body composition characteristics by multichannel bioimpedance analysis (BIA) InBody 720, as well as contractile characteristics of different muscle groups with tenziometric Dinamometry method. Based on the results of the factor analysis we found that regardless of the sex the most sensitive variable for partialization of absolute body isometric strength variable (ABiS) is partialization by allometric scaling (0.964 for females and 0.947 for males explained factor variance). However, in the case of absolute body isometric explosiveness (ABiE), the results of this study have demonstrated that partialization relative to skeletal muscle mass according to the body longitudinality - skeletal muscle mass index (SMMI) is the methodological choice disregarding the sex (0.982 for females and 0.980 for males explained factor variance). The results of the study have shown that for the purpose of scaling the maximal strength relative to body composition, the allometric method can be considered as a choice, while for the partialization of maximal isometric explosiveness skeletal muscle mass index is the best choice insensitive of the sex.

El objetivo de este estudio fue determinar la sensibilidad de distintos métodos de parcialización, en términos de diferentes índices de componentes corporales en relación a los indicadores de fuerza y explosividad. En la investigación participaron 187 sujetos que se dividieron según el sexo en dos grupos. Esta investigación consistió en medir las características de composición corporal mediante análisis de bioimpedancia multicanal (BIA) InBody 720, así como las características contráctiles de diferentes grupos musculares con el método de Dinamometría tenciométrica. Con base en los resultados del análisis factorial, encontramos que, independientemente del sexo, la variable más sensible para la parcialización de la variable de fuerza isométrica corporal absoluta (ABiS) fue la parcialización mediante escala alométrica (0,964 para las mujeres y 0,947 para los hombres). Sin embargo, en el caso de la explosividad isométrica corporal absoluta (ABiE), los resultados de este estudio han demostrado que la parcialización relativa a la masa del músculo esquelético según la longitudinalidad del cuerpo - índice de masa del músculo esquelético (SMMI) es la opción metodológica sin tener en cuenta el sexo (0,982 para las mujeres y 0,980 para los hombres). Los resultados del estudio han demostrado que para escalar la fuerza máxima en relación con la composición corporal, el método alométrico puede considerarse como una opción, mientras que para la parcialización de la explosividad isométrica máxima, el índice de masa del músculo esquelético es la mejor opción independiente del sexo.

Clinical Indicators of Bone Deterioration in Alcoholic Liver Cirrhosis and Chronic Alcohol Abuse: Looking beyond Bone Fracture Occurrence.

Although previous studies indicated that chronic alcohol abuse (CAA) and alcoholic liver cirrhosis (ALC) are associated with increased bone fragility, understanding bone fragility determinants is still modest in these individuals. We used a comprehensive individualized clinical fracture risk assessment approach (vertebral osteodensitometry, femoral osteodensitometry and geometry, and serum bone turnover biomarkers) to compare adult male patients with ALC who have not previously had femoral or vertebral fractures (n = 39), patients with CAA (without liver cirrhosis, n = 78) who have not previously had femoral or vertebral fractures and healthy age- and sex-matched controls (n = 43). Our data suggested that intertrochanteric bone mineral density was significantly lower in ALC and CAA patients than in controls. Also, the trabecular bone score was considerably lower in ALC patients compared with CAA and control individuals. The most significant inter-group differences in femoral geometry were noted on the femoral shaft. Patients with ALC and CAA have a higher 10-year risk of major osteoporotic fractures compared to the controls. Analysis of bone turnover biomarkers showed increased osteoprotegerin and beta-C-terminal telopeptide serum concentrations and decreased insulin growth factor-1 concentrations in patients with ALC compared to CAA and control groups. Our data revealed that bone alterations are present in patients with ALC and CAA even if they did not sustain a nontraumatic bone fracture, but it is also indicative that current bone-assessing clinical methods are not entirely reliable. Thus, future studies should focus on developing a reliable integrative clinical tool that can be used to accurately predict and prevent bone fracture occurrences in patients with ALC and CAA.

Differences in countermovement vertical jump force-time metrics between starting and non-starting professional male basketball players.

With force plates being widely implemented for neuromuscular performance assessment in sport-specific settings and various force-time metrics being able to differentiate athletes based on their performance capabilities, the purpose of the present study was to examine the differences in countermovement vertical jump (CVJ) characteristics between starting and non-starting professional male basketball players (e.g., ABA League). Twenty-three athletes (height = 199.2 ± 7.7 kg, body mass = 94.2 ± 8.2 kg, age = 23.8 ± 4.9 years) volunteered to participate in the present investigation. Upon completion of a standardized warm-up protocol, each athlete performed three maximal-effort CVJs without an arm swing while standing on a uni-axial force plate system sampling at 1,000 Hz. Independent t-tests were used to examine statistically significant differences (p < 0.05) in each force-time metric between starters (n = 10) and non-starters (n = 13). No significant differences in any of the CVJ force-time metrics of interest were observed between the two groups, during both the eccentric and concentric phases of the movement (i.e., impulse, duration, peak velocity, and mean and peak force and power). Moreover, starters and non-starters demonstrated similar performance on CVJ outcome (e.g., jump height) and strategy metrics (e.g., countermovement depth). Overall, these findings suggest that at the professional level of play, the ability to secure a spot in the starting lineup is not primarily determined by the players' CVJ performance characteristics.

Capillaria plica in Red Foxes (Vulpes vulpes) from Serbia: Epidemiology and Diagnostic Approaches to Urinary Capillariosis in Domestic Carnivores.

PURPOSE: This study aimed to determine the presence of C. plica in red foxes and to point out the possibilities of different diagnostic methods, that could be used for diagnosis of urinary capillariosis in domestic carnivores. METHODS: Seventeen red foxes from hunting grounds in the Kolubara District, Republic of Serbia, were examined in February 2019. Animals to be shot were selected based on the criteria of gamekeepers and the permission of the competent Ministry. After hunting, the foxes were examined by ultrasound and autopsied. After the necropsy, biochemical and sediment analysis of urine morphological identification of isolated adult parasites, and macroscopic/microscopic examination of the urinary bladder and kidney tissue were performed. RESULTS: Adults of C. plica were detected in 3/17 foxes (17.6%) by ultrasound imaging and in 6/17 foxes (35.3%) by necropsy examination. Parasite eggs were found in the urinary sediment of 9/17 foxes (52.9%). The predominant histopathologic changes were amyloid degeneration of the renal glomeruli and proximal tubules (8/17 foxes-47%) as well as acute cystitis (7/17 foxes-41.2%). The occurrence of C. plica was determined in 12/17 (70.6%) of the examined foxes. DISCUSSION: This study is the first record of the C. plica in red foxes in the Republic of Serbia. Diagnostic methods used in this study could make possible the early revealing of capillariosis in domestic carnivores and could provide reliable clinical and parasitological screening of suspect animals. CONCLUSION: The study presents the first report of urinary capillariosis in a fox population in Serbia. The established high prevalence of C. plica in foxes could presume its higher prevalence in domestic and wild carnivores in the future.

TReND in Africa: Toward a Truly Global (Neuro)science Community.

TReND is a volunteer-scientist run charity dedicated to promoting research and education on the African continent. Focusing on neuroscience, we discuss approaches to address some of the factors that currently stifle Africa's scientific development and our experience in implementing them.

Complete mitogenome data for the Serbian population: the contribution to high-quality forensic databases.

Mitochondrial genome (mtDNA) is a valuable resource in resolving various human forensic casework. The usage of variability of complete mtDNA genomes increases their discriminatory power to the maximum and enables ultimate resolution of distinct maternal lineages. However, their wider employment in forensic casework is nowadays limited by the lack of appropriate reference database. In order to fill in the gap in the reference data, which, considering Slavic-speaking populations, currently comprises only mitogenomes of East and West Slavs, we present mitogenome data for 226 Serbians, representatives of South Slavs from the Balkan Peninsula. We found 143 (sub)haplogroups among which West Eurasian ones were dominant. The percentage of unique haplotypes was 85%, and the random match probability was as low as 0.53%. We support previous findings on both high levels of genetic diversity in the Serbian population and patterns of genetic differentiation among this and ten studied European populations. However, our high-resolution data supported more pronounced genetic differentiation among Serbians and two Slavic populations (Russians and Poles) as well as expansion of the Serbian population after the Last Glacial Maximum and during the Migration period (fourth to ninth century A.D.), as inferred from the Bayesian skyline analysis. Phylogenetic analysis of haplotypes found in Serbians contributed towards the improvement of the worldwide mtDNA phylogeny, which is essential for the interpretation of the mtDNA casework.

Comparison of Three Ratiometric Temperature Readings from the Er3+ Upconversion Emission.

The emission of Er3+ provides three combinations of emission bands suitable for ratiometric luminescence thermometry. Two combinations utilize ratios of visible emissions (2H11/2→4I15/2 at 523 nm/ 4S3/2→4I15/2 at 542 nm and 4F7/2→4I15/2 at 485 nm/ 4S3/2→4I15/2 at 545 nm), while emissions from the third combination are located in near-infrared, e.g., in the first biological window (2H11/2→4I13/2 at 793 nm/ 4S3/2→4I13/2 at 840 nm). Herein, we aimed to compare thermometric performances of these three different ratiometric readouts on account of their relative sensitivities, resolutions, and repeatability of measurements. For this aim, we prepared Yb3+,Er3+:YF3 nanopowders by oxide fluorination. The structure of the materials was confirmed by X-ray diffraction analysis and particle morphology was evaluated from FE-SEM measurements. Upconversion emission spectra were measured over the 293-473 K range upon excitation by 980 nm radiation. The obtained relative sensitivities on temperature for 523/542, 485/542, and 793/840 emission intensity ratios were 1.06 ± 0.02, 2.03 ± 0.23, and 0.98 ± 0.10%K-1 with temperature resolutions of 0.3, 0.7, and 1.8 K, respectively. The study showed that the higher relative temperature sensitivity does not necessarily lead to the more precise temperature measurement and better resolution, since it may be compromised by a larger uncertainty in measurement of low-intensity emission bands.

Banat donkey, a neglected donkey breed from the central Balkans (Serbia).

The dominant donkey breed in the Balkans is the mid-sized Balkan donkey with a grey to chocolate coat color. Local breeders from Serbia, however, still maintain a few larger individuals of a lighter coat color, named Banat donkey, and speculate that they are descendants of a Spanish donkey heard that had been transferred to the Banat region by the Hapsburg Queen Maria Theresa in the XVIII century for a specific purpose, to work in local vineyards. We have previously found a unique nuclear gene-pool and a prevalence of mitochondrial Clade 2 haplotypes in several such animals. In this study, we: (i) perform a comparative analysis of 18 morphological traits of the Banat donkey (seven individuals), Balkan donkey (53 individuals from two sub-populations of this breed) and the potential hybrids (eight individuals), and demonstrate the morphological distinctiveness of the Banat donkey, highlighting the diagnostic traits for distinguishing the breed: hip height, croup width, body length and chest depth; (ii) re-analyse published nuclear microsatellite data for these groups, and reveal that, although severely depopulated, the genetically distinct Banat donkey is not severely affected by the loss of genetic diversity and inbreeding; (iii) demonstrate that previously published Banat donkey mitochondrial haplotypes, analyzed genealogically together with those reported in ancient and modern individuals from Spain, Italy, Turkey, Cyprus and Africa, are shared with three Spanish breeds and individuals belonging to Amiata and some other Italian breeds. A unique morphological feature present in Banat and Somali wild donkeys, but also in Amiata donkeys, black stripes on legs, suggests that the origin of Clade 2 donkeys may be much more complex than previously thought. Actions to preserve the Banat donkey, a valuable but critically endangered genetic resource (<100 individuals), are urgent.

Genomic data provide new insights on the demographic history and the extent of recent material transfers in Norway spruce.

Primeval forests are today exceedingly rare in Europe, and transfer of forest reproductive material for afforestation and improvement has been very common, especially over the last two centuries. This can be a serious impediment when inferring past population movements in response to past climate changes such as the last glacial maximum (LGM), some 18,000 years ago. In the present study, we genotyped 1,672 individuals from three Picea species (P. abies, P. obovata, and P. omorika) at 400K SNPs using exome capture to infer the past demographic history of Norway spruce (P. abies) and estimate the amount of recent introduction used to establish the Norway spruce breeding program in southern Sweden. Most of these trees belong to P. abies and originate from the base populations of the Swedish breeding program. Others originate from populations across the natural ranges of the three species. Of the 1,499 individuals stemming from the breeding program, a large proportion corresponds to recent introductions from mainland Europe. The split of P. omorika occurred 23 million years ago (mya), while the divergence between P. obovata and P. abies began 17.6 mya. Demographic inferences retrieved the same main clusters within P. abies than previous studies, that is, a vast northern domain ranging from Norway to central Russia, where the species is progressively replaced by Siberian spruce (P. obovata) and two smaller domains, an Alpine domain and a Carpathian one, but also revealed further subdivision and gene flow among clusters. The three main domains divergence was ancient (15 mya), and all three went through a bottleneck corresponding to the LGM. Approximately 17% of P. abies Nordic domain migrated from P. obovata ~103K years ago, when both species had much larger effective population sizes. Our analysis of genomewide polymorphism data thus revealed the complex demographic history of Picea genus in Western Europe and highlighted the importance of material transfer in Swedish breeding program.

Capture the vertebral fracture: Risk factors as a prediction.

OBJECTIVE: Vertebral fractures are the most common osteoporotic fractures occurring due to low bone mineral density, as well as other risk factors. The aim of the paper is to investigate risk factors for vertebral osteoporotic fracture occurrence in postmenopausal women. METHODS: Retrospective analysis of data pertaining to 651 postmenopausal women obtained from the National Osteoporosis Registry of Serbia was conducted. Further analyses were performed on 217 osteoporotic women identified from those records, whereby those in the experimental group (n= 110) had a vertebral fracture, while those assigned to the control group (n= 107) did not. The two groups were comparable in terms of age (t= 0.450; p> 0.01). Risk factors that could serve as the best predictors of vertebral fracture occurrence were investigated. Multivariate logistic regression analysis was used for testing effect of several factors on vertebral fracture occurrence as the dependent variable. RESULTS: Patients that have never suffered a vertebral fracture had a significantly higher bone mineral density (t= 8.161; p< 0.01) in comparison to those with a verified vertebral fracture. Factors that significantly contributed to the risk of vertebral fracture were presence of kyphosis (OR 708.338; 95% CI 19.238-26.081.950), use of glucocorticoids (OR 87.618; 95% CI 9.175-836.707), and presence of comorbidities (OR 7.327; 95% CI 1.500-35.793). Moreover, a unit increase in body mass index (BMI) was found to lower the probability of vertebral fracture by a factor of 0.846. Women that entered menopause later have lower chance of suffering a vertebral fracture (OR = 0.539; 95% CI 0.400-0.726). CONCLUSION: Lower body mass index, presence of kyphosis, use of glucocorticoids, early menopause onset, and presence of comorbidities are the factors that contribute the most to vertebral osteoporotic fracture occurrence.

Honey bee viruses in Serbian colonies of different strength.

Protection of honey bees is of great economic importance because of their role in pollination. Crucial steps towards this goal are epidemiological surveys of pathogens connected with honey bee losses. In this study deformed wing virus (DWV), chronic bee paralysis virus (CBPV), acute bee paralysis virus (ABPV) and sacbrood virus (SBV) were investigated in colonies of different strength located in five regions of Serbia. The relationship between colony strength and virus occurrence/infection intensity were assessed as well as the genetic relationship between virus sequences from Serbia and worldwide. Real-time RT-PCR analyses detected at least one virus in 87.33% of colonies. Single infection was found in 28.67% colonies (21.33%, 4.00%, 2.67% and 0.67% in cases of DWV, ABPV, SBV and CBPV, respectively). In the majority of colonies (58.66%) more than one virus was found. The most prevalent was DWV (74%), followed by ABPV, SBV and CBPV (49.30%, 24.00% and 6.70%, respectively). Except for DWV, the prevalence of the remaining three viruses significantly varied between the regions. No significant differences were found between colony strength and either (i) the prevalence of DWV, ABPV, SBV, CBPV and their combinations, or (ii) DWV infection levels. The sequences of honey bee viruses obtained from bees in Serbia were 93-99% identical with those deposited in GenBank.