[A surface membrane study of human cell lines with different interferon sensitivities]. / Issledovanie poverkhnostnykh membran v liniiakh chelovecheskikh kletok s razlichnoi chuvstvitel'nost'iu k interferonu.

A comparative study of surface membranes of human cell J-96 and J-48 cultures with different sensitivity to alpha/beta interferon (IF). Reduced sensitivity of J-41 cells to IF-alpha/beta was found to be accompanied by a loss of highly specific receptors for IF-alpha, the lack of changes in the cell surface structures upon treatment with IF-alpha/beta, reduced intensity of cell fusion upon successive treatment with IF and polyethylene glycol. The results are discussed in connection with the observed changes in the activity of superoxide dismutase in J-96 and J-41 cell lines after treatment with IF.

[Interferon-specific cellular receptors in cultured human cells, differing in sensitivity to alpha-interferon]. / Interferon-spetsificheskie kletochnye retseptory v kul'turakh chelovecheskikh kletok, razlichaiushchikhsiia po chuvstvitel'nosti k alpha-interferonu.

The interferon-specific cellular receptors in human cells cultures differing in sensitivity to alpha-interferon have been studied. The J-41 cells resistant to alpha-interferon are practically devoid of receptors highly specific to alpha-interferon. The coefficient of equilibrium and the number of receptors analyzed after Scatchard for J-96 culture of cells are 15.6 x 10(11) M-1 and 210 +/- 90, respectively. Evidently, resistance of J-41 cells to alpha-interferon is connected with the absence of interferon receptors and, as a consequence, inability to interferon-receptor interaction.

[Induction of interferon-specific enzymes in cultures of cells sensitive and resistant to interferon]. / Induktsiia interferonspetsificheskikh fermentov v kul'turakh, chuvstvitel'nykh i rezistentnykh k interferonu.

Induction of 2'-5'-oligoadenylatesynthetase (2-5A synthetase) by interferons and theophylline by means of activation of cAMP-system in interferon susceptible and resistant cell lines were studied. In interferon resistant cell lines the basal activity of 2-5A synthetase exceeded the level of the same enzyme in interferon susceptible cell lines. Activity of 2-5A synthetase is increased in interferon susceptible cell lines by interferon treatment, but the activity of the enzyme is not altered in interferon resistant cell lines. Among the studied cell lines the induction of 2-5A synthetase by theophylline was possible only in L929 cell line. The common mechanism for the absence of 2-5A synthetase induction by interferon and theophylline in interferon resistant cells is discussed.

[Karyological characteristics of a new continuous cell line of human hypernephroma]. / Kariologicheskaia kharakteristika novoi perevivaemoi linii kletok gipernefromy cheloveka.

A new stable human hypernephroma cell line has been characterized by differential staining R-, C- and Ag-I-techniques. The karyotype of the cultivated hypernephroma cells has been designed using the statistical analysis and karyotype reconstruction methods. The specific chromosomal markers of the cell line are described.