RESUMO
It was shown that bidistilled modified water substantially enhances the hydrolysis of the peptide the bovine insulin B-chain. The exposure of the peptide to bidistilled modified water for 20 hours at room temperature leads to an almost complete hydrolysis of its molecule into fragments that differ from the initial molecule in elution time from a column in high-performance liquid chromatography.
Assuntos
Insulina/química , Água , Animais , Bovinos , Cromatografia Líquida de Alta Pressão , HidróliseRESUMO
The effect of bidistilled modified water obtained by treating bidistilled water with static electric field in the cathode compartment of a diaphragm electrolyzer on the production of tumor necrosis factor and activity of natural killer cells of mice was studied. It was found that bidistilled modified water added to cultured murine macrophages increases the tumor necrosis factor production 2.5-3-fold. A similar degree of stimulation of tumor necrosis factor production was observed after peroral administration of bidistilled modified water for 10-25 days. The activity of natural killer cells under these conditions increased by 150%. The stimulation of these immunity system components responsible for the resistance of the organism to tumors led to a suppression of tumor growth in tumor-bearing mice receiving bidistilled modified water.
Assuntos
Adjuvantes Imunológicos/uso terapêutico , Carcinoma de Ehrlich/tratamento farmacológico , Fator de Necrose Tumoral alfa/biossíntese , Água , Adjuvantes Imunológicos/química , Animais , Carcinoma de Ehrlich/imunologia , Divisão Celular/efeitos dos fármacos , Células Cultivadas , Eletrólise , Humanos , Células Matadoras Naturais/efeitos dos fármacos , Células L , Macrófagos Peritoneais/metabolismo , Masculino , Camundongos , Baço/efeitos dos fármacos , Baço/patologia , Água/químicaRESUMO
It was shown that the exposure of a recombinant reverse transcriptase of human immunodeficiency virus HIV-1 to bidistilled modified water substantially decreases the RNA-dependent DNA polymerase activity of this enzyme.
Assuntos
Inibidores Enzimáticos/química , HIV-1/química , DNA Polimerase Dirigida por RNA/química , Água/química , Depressão Química , Proteínas Recombinantes/químicaRESUMO
It was shown that bidistilled modified water induces a marked decrease in the intensity of intrinsic fluorescence of bovine serum albumin and increases the binding of this protein to the fluorescent probe 1.8 ANS. These effects can be interpreted as a denaturing action of bidistilled modified water on the protein and a change in its conformational state, which is probably caused by changes in the microenvironment of the protein molecule. In addition, a substantial increase in the intrinsic fluorescence of bidistilled modified water, as compared with that of distilled water, was found.