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OBJECTIVE: To evaluate the effects of Prostate artery embolization (PAE) and open simple prostatectomy (OP) on lower urinary tract symptoms and urodynamic parameters in subjects with prostate size >80cc³. METHODS: PoPAE study (OP or PAE) was a randomized, open-label controlled trial performed between January 2020 and May 2022. Subjects with large prostates (>80cc³), urodynamic parameters meeting obstruction criteria (Bladder Outlet Obstruction Index-BOOI>40), and good detrusor function (Bladder contractility index>100) were included. The primary and co-primary endpoints were the variation in peak flow rate on uroflowmetry (Qmax) and BOOI. The secondary endpoints were the IPSS and ultrasonographic changes. RESULTS: Twenty three and 25 subjects underwent PAE and OP were evaluated, respectively. At baseline, the 2 groups have shown similar clinical, radiological, laboratory, and urodynamic parameters. After 6 months, Qmax improved 8,3 ± 4.17 mL/sec in PAE and 15.1 ± 8.04 mL/sec in OP (mean difference 6.78 in favor of PE; P = .012 [CI -9.00 to -3.00]). After treatment, 88% of those men underwent OP were classified as unobstructed or equivocal (BOOi<40). On the other hand, 70% of subjects underwent PAE remained obstructed (BOOI>40) and none of them shifted to unobstructed status (BOOI<20). It was observed a similar reduction in IPSS and PVR in both groups. CONCLUSION: PAE was inferior to conventional surgery for releasing BOO and improving peak urinary flow in large prostates. Nevertheless, PAE was able to improve symptoms and PVR, and might be an alternative method in selected patients.
Assuntos
Embolização Terapêutica , Sintomas do Trato Urinário Inferior , Próstata , Prostatectomia , Hiperplasia Prostática , Urodinâmica , Humanos , Masculino , Prostatectomia/métodos , Próstata/irrigação sanguínea , Próstata/diagnóstico por imagem , Embolização Terapêutica/métodos , Idoso , Hiperplasia Prostática/cirurgia , Hiperplasia Prostática/complicações , Hiperplasia Prostática/terapia , Pessoa de Meia-Idade , Sintomas do Trato Urinário Inferior/etiologia , Sintomas do Trato Urinário Inferior/terapia , Sintomas do Trato Urinário Inferior/diagnóstico , Sintomas do Trato Urinário Inferior/fisiopatologia , Tamanho do Órgão , Obstrução do Colo da Bexiga Urinária/etiologia , Obstrução do Colo da Bexiga Urinária/terapia , Obstrução do Colo da Bexiga Urinária/fisiopatologia , Resultado do Tratamento , Artérias/diagnóstico por imagemRESUMO
Objective Semiquantitative and automated measurement of nuclear material removal and cell infiltration in decellularized tendon scaffolds (DTSs). Method 16 pure New Zealand rabbits were used, and the gastrocnemius muscle tendon was collected bilaterally from half of these animals (16 tendons collected); 4 were kept as control and 12 were submitted to the decellularization protocol (DTS). Eight of the DTSs were used as an in vivo implant in the experimental rotator cuff tear (RCT) model, and the rest, as well as the controls, were used in the semiquantitative and automated evaluation of nuclear material removal. The eight additional rabbits were used to make the experimental model of RCT and subsequent evaluation of cellular infiltration after 2 or 8 weeks, within the DTS. Results The semiquantitative and automated analysis used demonstrated a removal of 79% of nuclear material ( p < 0.001 and power > 99%) and a decrease of 88% (p < 0.001 and power >99%) in the area occupied by nuclear material after the decellularization protocol. On cell infiltration in DTS, an increase of 256% (p < 0.001 and power >99%) in the number of cells within the DTS was observed in the comparison between 2 and 8 weeks postoperatively. Conclusion The proposed semiquantitative and automated measurement method was able to objectively measure the removal of nuclear material and cell infiltration in DTS.
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Abstract Objective Semiquantitative and automated measurement of nuclear material removal and cell infiltration in decellularized tendon scaffolds (DTSs). Method 16 pure New Zealand rabbits were used, and the gastrocnemius muscle tendon was collected bilaterally from half of these animals (16 tendons collected); 4 were kept as control and 12 were submitted to the decellularization protocol (DTS). Eight of the DTSs were used as an in vivo implant in the experimental rotator cuff tear (RCT) model, and the rest, as well as the controls, were used in the semiquantitative and automated evaluation of nuclear material removal. The eight additional rabbits were used to make the experimental model of RCT and subsequent evaluation of cellular infiltration after 2 or 8 weeks, within the DTS. Results The semiquantitative and automated analysis used demonstrated a removal of 79% of nuclear material (p< 0.001 and power > 99%) and a decrease of 88% (p < 0.001 and power >99%) in the area occupied by nuclear material after the decellularization protocol. On cell infiltration in DTS, an increase of 256% (p < 0.001 and power >99%) in the number of cells within the DTS was observed in the comparison between 2 and 8 weeks postoperatively. Conclusion The proposed semiquantitative and automated measurement method was able to objectively measure the removal of nuclear material and cell infiltration in DTS.
Resumo Objetivo Mensuração semiquantitativa e automatizada da remoção de material nuclear e da infiltração celular em scaffolds tendinosos descelularizados (STDs). Método Foram utilizados 16 coelhos Nova Zelândia puros, sendo o tendão do músculo gastrocnêmio coletado bilateralmente de metade destes animais (16 tendões coletados); 4 foram mantidos como controle e 12 foram submetidos ao protocolo de descelularização (STD). Dos STDs, 8 foram utilizados como implante in vivo no modelo experimental de lesão do manguito rotador (LMR) e os restantes, assim como os controles, foram utilizados na avaliação semiquantitativa e automatizada da remoção de material nuclear. Os oito coelhos adicionais foram utilizados na confecção do modelo experimental de LMR e posterior avaliação da infiltração celular após 2 ou 8 semanas, dentro do STD. Resultados A análise semiquantitativa e automatizada utilizada demonstrou uma remoção de 79% do material nuclear (p< 0,001 e poder > 99%) e uma diminuição de 88% (p< 0,001 e poder > 99%) na área ocupada por material nuclear após o protocolo de descelularização. Sobre a infiltração celular no STD, foi observado um aumento de 256% (p< 0,001 e poder > 99%) no número de células dentro do STD na comparação entre 2 e 8 semanas de pós-operatório. Conclusão O método de mensuração semiquantitativo e automatizado proposto foi capaz de mensurar objetivamente a remoção de material nuclear e a infiltração celular no STD.
Assuntos
Animais , Coelhos , Tendões , Engenharia Tecidual , Medicina Regenerativa , Matriz Extracelular , Alicerces TeciduaisRESUMO
Objective Histological and macroscopic evaluation of the healing process of acute lesions of the femoral rectus muscle using stem cells derived from adipose tissue-derived stem cells (ADSCs). Method An experimental study was conducted with 18 hind legs of New Zealand rabbits, which were divided into three study groups according to the intervention to be performed. In group I, no surgical procedure was performed; in group II-SHAN, the experimental lesion was performed without any additional intervention protocol; in group III-Intervention, the addition of ADSCs was performed in the same topography of the experimental lesion. After the proposed period, 2 weeks, the material was collected and submitted to macroscopic and histological evaluation. Results The quantitative analysis showed that the addition of ADSCs is related to the reduction of inflammatory cells in the 2-week evaluation (164.2 cells in group II - SHAN to 89.62 cells in group III - ADSC). The qualitative analysis of the slides with Picrosirius red, noticed an increase in orange/yellow fibers in group III - ADSC, which evidences a final healing process. The macroscopic evaluation found no difference between the groups. Conclusion The use of ADSCs in the treatment of acute muscle injury presented histological advantages when compared to their non-use.
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Abstract Objective Histological and macroscopic evaluation of the healing process of acute lesions of the femoral rectus muscle using stem cells derived from adipose tissue-derived stem cells (ADSCs). Method An experimental study was conducted with 18 hind legs of New Zealand rabbits, which were divided into three study groups according to the intervention to be performed. In group I, no surgical procedure was performed; in group II—SHAN, the experimental lesion was performed without any additional intervention protocol; in group III—Intervention, the addition of ADSCs was performed in the same topography of the experimental lesion. After the proposed period, 2 weeks, the material was collected and submitted to macroscopic and histological evaluation. Results The quantitative analysis showed that the addition of ADSCs is related to the reduction of inflammatory cells in the 2-week evaluation (164.2 cells in group II - SHAN to 89.62 cells in group III - ADSC). The qualitative analysis of the slides with Picrosirius red, noticed an increase in orange/yellow fibers in group III - ADSC, which evidences a final healing process. The macroscopic evaluation found no difference between the groups. Conclusion The use of ADSCs in the treatment of acute muscle injury presented histological advantages when compared to their non-use.
Resumo Objetivo Avaliação histológica e macroscópica do processo de cicatrização das lesões agudas do músculo reto femoral, com utilização de células-tronco derivadas de tecido adiposo (ADSCs, na sigla em inglês). Método Foi realizado um estudo experimental com 18 patas traseiras de coelhos Nova Zelândia, que foram divididos em três nos grupos de estudo de acordo com a intervenção a ser realizada. No grupo I não foi realizado procedimento cirúrgico; no grupo II - SHAN foi realizado a lesão experimental sem nenhum protocolo de intervenção adicional; e no grupo III - Intervenção foi realizado a adição de ADSCs na mesma topografia onde foi realizada a lesão experimental. Após o período proposto, 2 semanas, o material foi coletado, submetido a avaliação macroscópica e histológica. Resultados A análise quantitativa demonstrou que a adição de ADSCs está relacionada com a diminuição de células inflamatórias na avaliação com 2 semanas (164,2 células no grupo II - SHAN para 89,62 células no grupo III - ADSC). A análise qualitativa das lâminas coradas com Picrosírius red demonstrou um aumento das fibras de cor laranja/amarela no grupo III - ADSC, o que evidencia um processo final de cicatrização. A avaliação macroscópica não encontrou diferença entre os grupos. Conclusão A utilização de ADSCs no tratamento de lesão muscular aguda apresentou vantagens histológicas quando comparada a sua não utilização.
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Animais , Coelhos , Regeneração , Medicina Regenerativa , Células-Tronco Mesenquimais , Músculos , Doenças MuscularesRESUMO
Bladder and urinary sphincter malfunctioning that results from some change in the central and/or peripheral nervous system is defined as neurogenic bladder. The urinary tract symptoms that can be related to its filling, emptying, or both have a significant impact on the quality of life of individuals. The present review was based on the document prepared for the public health system in Brazil as a treatment guidelines proposal. Material and Methods: Survey questions were structured as per PICO (Population, Intervention, Control, and Outcome). Search strategies were defined and performed in the MEDLINE/Pubmed, Embase, Epistemonikos and Google Scholar databases. The selection of articles followed the evidence hierarchy concept; evidence body was identified, and the quantitative study data were extracted. The quality of evidence and grade of recommendation were qualitatively assessed according to GRADE (Grading of Recommendations, Assessment, Development and Evaluations). Results: A total of 2.707 articles were identified, with 49 of them being selected to compose the basis for this review. Neurogenic bladder treatments were classified according to their focus on filling or emptying symptoms and sub- classified in pharmacological and surgical treatments. Conclusion: Treatment guidelines are important tools for the public health system to promote the best practice when treating neurogenic bladder patients.
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Humanos , Sistema Urinário , Bexiga Urinaria Neurogênica/terapia , Qualidade de Vida , Bexiga Urinária , Inquéritos e QuestionáriosRESUMO
Mesenchymal stem cells (MSCs), such as adipose-derived stem cells (ADSCs) and skeletal muscle-derived stem cells (MDSCs), are potential sources for cell-based therapeutic strategies. However, there is little knowledge about the lipid composition of these stem cells and the mechanisms of their differentiation. Lipids have important biological and physiological functions that are critical for understanding the regulation and control of stem cell fate. This study sought to analyze the lipidome of rabbit ADSCs and MDSCs and their adipogenic and osteogenic differentiation. The MSCs were isolated and were characterized by flow cytometry. Lipids were extracted from both MSCs and differentiated cells, and the lipids were subsequently analyzed with a hybrid triple quadrupole time-of-flight mass spectrometer. The results showed a total of 1687 lipid species. MSCs exhibited different lipid profiles as well as changes in lipid composition after differentiation. Furthermore, the expression levels of N-acyl-phosphatidylethanolamine (NAPE) 54:7+NH4 (-FA 17:0(NH4)) and phosphatidylcholine (PC) 42:6+Na were higher in the adipogenic lineages in of both MSC types, and NAPE 58:2+NH4 (-FA 17:0 (NH4)) and NAPE 56:2+NH4 (-FA 17:0 (NH4)) had higher levels in the osteogenic lineages, suggesting lipid similarities in cells differentiated from different stem cell sources.
Assuntos
Diferenciação Celular , Lipidômica , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/metabolismo , Adipogenia , Tecido Adiposo/citologia , Animais , Regulação da Expressão Gênica , Osteogênese , CoelhosRESUMO
The Guidelines Project, an initiative of the Brazilian Medical Association, aims to combine information from the medical field in order to standardize producers to assist the reasoning and decision-making of doctors. The information provided through this project must be assessed and criticized by the physician responsible for the conduct that will be adopted, depending on the conditions and the clinical status of each patient.
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Acetanilidas/administração & dosagem , Agonistas de Receptores Adrenérgicos beta 3/administração & dosagem , Antagonistas Muscarínicos/administração & dosagem , Tiazóis/administração & dosagem , Bexiga Urinária Hiperativa/tratamento farmacológico , Antidepressivos/administração & dosagem , Benzilatos/administração & dosagem , Benzofuranos/administração & dosagem , Brasil , Tomada de Decisão Clínica , Quimioterapia Combinada , Humanos , Ácidos Mandélicos/administração & dosagem , Nortropanos/administração & dosagem , Pirrolidinas/administração & dosagem , Succinato de Solifenacina/administração & dosagem , Tartarato de Tolterodina/administração & dosagemRESUMO
The Guidelines Project, an initiative of the Brazilian Medical Association, aims to combine information from the medical field in order to standardize producers to assist the reasoning and decision-making of doctors. The information provided through this project must be assessed and criticized by the physician responsible for the conduct that will be adopted, depending on the conditions and the clinical status of each patient.
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Cistite Intersticial/tratamento farmacológico , Administração Intravesical , Toxinas Botulínicas Tipo A/uso terapêutico , Brasil , Sulfatos de Condroitina/uso terapêutico , Tomada de Decisão Clínica , Dimetil Sulfóxido/uso terapêutico , Diterpenos/uso terapêutico , Humanos , Ácido Hialurônico/uso terapêutico , Lidocaína/uso terapêutico , Mycobacterium bovis , Poliéster Sulfúrico de Pentosana/uso terapêutico , Resultado do TratamentoRESUMO
The Guidelines Project, an initiative of the Brazilian Medical Association, aims to combine information from the medical field in order to standardize producers to assist the reasoning and decision-making of doctors. The information provided through this project must be assessed and criticized by the physician responsible for the conduct that will be adopted, depending on the conditions and the clinical status of each patient.
Assuntos
Humanos , Tiazóis/administração & dosagem , Antagonistas Muscarínicos/administração & dosagem , Bexiga Urinária Hiperativa/tratamento farmacológico , Agonistas de Receptores Adrenérgicos beta 3/administração & dosagem , Acetanilidas/administração & dosagem , Pirrolidinas/administração & dosagem , Benzilatos/administração & dosagem , Benzofuranos/administração & dosagem , Brasil , Quimioterapia Combinada , Tartarato de Tolterodina/administração & dosagem , Succinato de Solifenacina/administração & dosagem , Tomada de Decisão Clínica , Ácidos Mandélicos/administração & dosagem , Antidepressivos/administração & dosagem , Nortropanos/administração & dosagemRESUMO
The Guidelines Project, an initiative of the Brazilian Medical Association, aims to combine information from the medical field in order to standardize producers to assist the reasoning and decision-making of doctors. The information provided through this project must be assessed and criticized by the physician responsible for the conduct that will be adopted, depending on the conditions and the clinical status of each patient.
Assuntos
Humanos , Cistite Intersticial/tratamento farmacológico , Poliéster Sulfúrico de Pentosana/uso terapêutico , Administração Intravesical , Brasil , Dimetil Sulfóxido/uso terapêutico , Sulfatos de Condroitina/uso terapêutico , Resultado do Tratamento , Toxinas Botulínicas Tipo A/uso terapêutico , Diterpenos/uso terapêutico , Tomada de Decisão Clínica , Ácido Hialurônico/uso terapêutico , Lidocaína/uso terapêutico , Mycobacterium bovisRESUMO
A estimulaçäo magnética perineal (EMP) para o tratamento da incontinência urinária feminina surgiu recentemente como uma alternativa näo invasiva. EMP induz uma despolarizaçäo dos nervos do assoalho pélvico gerando contraçäo da musculatura da regiäo, sem os incovenientes e, supostamente, com eficácia semelhante á estimulaçäo elétrica. Como a EMP é uma modalidade de tratamento recente, existem poucos estudos clínicos relacionados ao seu uso. Realizamos um estudo clínico prospectivo com 91 mulheres com incontinência urinária demonstrável, O tratamento consistia de l6 sessöes de 20 minutos de EMP, sendo 10 minutos de estimulaçäo a baixa freqüência (5Hz) e 10 minutos de estimulaçäo a alta freqüência (50Hz), com intervalo mínimo de 36 horas entre as aplicaçöes. A avaliaçäo dos resultados foi realizada em duas etapas consecutivas e programadas, a primeira com 50 pacientes e a segunda com 41 pacientes. Nas duas etapas as pacientes foram avaliadas antes e após o tratamento, através de uma completa história clínica, do exame físico e do preenchimento de um diário miccional de 3 dias consecutivos e de um escore de qualidade de vida específico para incontinência urinária que variava de 22 á 110. Na segunda etapa acrescentamos o estudo urodinâmico completo antes e após o tratamento. O estudo urodinâmico antes do tratamento mostrou 24(58,5 por cento) mulheres com incontinências urinária pura de esforço(IUE) e 17(41,5 por cento) pacientes com incontinência urinária mista ou hiperatividade detrusora pura. As últimas foram divididas em 2 grupos: Grupo I - Nove (22 por cento) mulheres com IUE e contraçäo vesical involuntária parcialmente inibida sem perdas urinárias e Grupo II - Oito (19,5 por cento) mulheres com forte contraçäo involuntária levando a perda urinária. A análise dos resultados da primeira etapa revelou um aumento médio no escore de qualidade de vida de 39 por cento (p<0,001), uma diminuiçäo no número de absorventes utilizados por dia de 42 por cento (p<0,001) e uma diminuiçäo no número de perdas de 60 por cento (p<0,001). A avaliaçäo urodinâmica após o tratamento mostrou um aumento 36 por cento no volume médio do primeiro desejo miccional (p=0,0024) e 17,7 por cento no volume médio do desejo normal de micçäo (p=0,005). O aumento médio napressäo de perda foi 24 por cento (p=0,001). Dez mulheres tornaram-se secas(24 por cento). Todas pacientes que tornaram-se secas apresentavam pressäo de perda antes do tratamento maior que 80cm de H2O. Sete pacientes...