Correction: Biogenic sunflower oil-chitosan decorated fly ash nanocomposite film using white shrimp shell waste: Antibacterial and immunomodulatory potential.

[This corrects the article DOI: 10.1371/journal.pone.0282742.].

Potent and Versatile Biogenically Synthesized Alumina/Nickel Oxide Nanocomposite Adsorbent for Defluoridation of Drinking Water.

In the present work, an extract of Salvia officinalis leaves was used to synthesize an alumina/NiO nanocomposite by the coprecipitation method. First, the shape and surface content of the synthesized adsorbent were determined. Scanning electron microscopy images showed the production of nanospheres and nanorods with sizes between 35 and 50 nm. X-ray diffraction measurement revealed strong, high-intensity peaks, confirming the preparation of a highly crystalline alumina/nickel oxide nanocomposite. Then, the pure nanoalumina, nickel oxide, and functionalized alumina/nickel oxide nanocomposite for water defluoridation were investigated under various conditions, for example, stirring period, pH, and initial fluoride concentration. Defluoridation with greener alumina, nickel oxide, and alumina/nickel oxide nanocomposite lasted 120 min at adsorbent dosages of 0.8 g/L in a pH 7 solution. The adoption process for the three sorbents matches the Langmuir adsorption isotherm. The process dynamics were explored using pseudo-second-order and first-order kinetics. The water quality after treatment met drinking water requirements, proving the viability of using nanoparticles for drinking water defluoridation. This work confirmed effective water defluoridation in the crystalline phase using synthesized nanoalumina, nickel oxide, and their nanocomposite, which highlights their importance for future drinking water defluoridation.

Design, Synthesis, and Evaluation of Novel Magnetic Nanoparticles Combined with Thiophene Derivatives for the Removal of Cr(VI) from an Aqueous Solution.

Most heavy metals are harmful to human health and the environment, even at extremely low concentrations. In natural waters, they are usually found only in trace amounts. Researchers are paying great attention to nanotechnology and nanomaterials as viable solutions to the problem of water pollution. This research focuses on the synthesis of organic thiophene derivatives that can be used as grafted ligands on the surface of silica-coated iron oxide nanoparticles to remove Cr(VI) chromium ions from water. The Vilsmeier-Haack reaction allows the formation of aldehyde groups in thiophene derivatives, and the resulting products were characterized by the FT-IR, NMR, and GC-MS. Schiff base is used as a binder between organic compounds and nanoparticles by the reaction of aldehyde groups in thiophene derivatives and amine groups on the surface of coated iron oxide nanoparticles. Schiff base functionalized Fe3O4 composites (MNPs@SiO2-SB-THCA) and (MNPs@SiO2-SB-THCTA) were successfully synthesized by homogeneous and heterogeneous methods and characterized by a combination of FT-IR, transmission electron microscopy, X-ray photoelectron spectroscopy, and thermogravimetric analysis. The adsorption studies, kinetic modeling, adsorption isotherms, and thermodynamics of the two materials, MNPs@SiO2-SB-THCA and MNPs@SiO2-SB-THCTA, were investigated for the removal of Cr(VI) from water at room temperature and at 50 mg/L. The high adsorption capacity at pH 6 for MNPs@SiO2-SB-THCTA was 15.53 mg/g, and for MNPs@SiO2-SB-THCA, it was 14.31 mg/g.

Charge Transfer Copper Chelating Complex and Biogenically Synthesized Copper Oxide Nanoparticles Using Salvia officinalis Laves Extract in Comparative Spectrofluorimetric Estimation of Anticancer Dabrafenib.

Cancer is a broad category of disease that can affect virtually any organ or tissue in the body when abnormal cells grow uncontrollably, invade surrounding tissue, and/or spread to other organs. Dabrafenib is indicated for the treatment of adult patients with advanced non-small cell lung cancer. In the present study, two newly developed spectrofluorimetric probes for the detection of the anticancer drug Dabrafenib (DRF) in its authentic and pharmaceutical products using an ecologically synthesized copper oxide nanoparticle (CuONPs) from Salvia officinalis leaf extract and a copper chelate complex are presented. The first system is based on the influence of the particular optical properties of CuONPs on the enhancement of fluorescence detection. The second system, on the other hand, acts through the formation of a copper charge transfer complex. Various spectroscopic and microscopic studies were performed to confirm the environmentally synthesized CuONPs. The fluorescence detections in the two systems were measured at λex 350 and λem of 432 nm. The results showed the linear concentration ranges for the DRF-CuONPs-SDS and DRF-Cu-SDS complexes were determined to be 1.0-500 ng mL- 1 and 1.0-200 ng mL- 1, respectively. FI = 1.8088x + 21.418 (r = 0.9997) and FI = 2.7536x + 163.37 (r = 0.9989) were the regression equations. The lower detection and quantification limits for the aforementioned fluorescent systems were determined to be 0.4 and 0.8 ng mL- 1 and 1.0 ng mL- 1, respectively. The results also showed that intra-day DRF assays using DRF-CuONPs-SDS and DRF-Cu(NO3)2-SDS systems yielded 0.17% and 0.54%, respectively. However, the inter-day assay results for the above systems were 0.27% and 0.65%, respectively. The aforementioned two systems were effectively used in the study of DRF with excellent percent recoveries of 99.66 ± 0.42% and 99.42 ± 0.56%, respectively. Excipients such as magnesium stearate, titanium dioxide, red iron oxide, and silicon dioxide used in pharmaceutical formulations, as well as various common cations, amino acids, and sugars, had no effect on the detection of compound.

Functionalized gum acacia-activated carbon-CaO/NiO nanocomposite for potential photocatalytic removal of organic pollutants from water.

A functionalized gum acacia-activated carbon-CaO/NiO nanocomposite was synthesized using an eco-friendly sol-gel method. The formed nanocomposite was designed to apply various light sources to enhance the improved removal of organic dyes such as methylene blue, methyl orange, methyl red, and rhodamine B from aqueous media. The band gap energies of CaO, NiO nanoparticles and gum acacia-activated carbon were 3.54, 4.28, and 5.34 eV, respectively, corresponding to a reflection edge of 350, 290, and 232 nm, respectively. The surface area of the synthesized nanocomposite was measured to be 17.892 m2 g-1. Sunlight and 20 mg L-1 of the nanocomposite quenched the dyes (methylene blue, 99.7%; methyl orange, 98.3%; methyl red, 96.7%; and rhodamine B, 93.5%) after 120, 100, 100, and 75 min of irradiation, respectively. However, after 80, 100, 100, and 75 min, the percentage of dyes under UV light irradiation was 98.6%, 95.8%, 98.4%, and 94.2% for methylene blue, methyl orange, methyl red, and rhodamine B, respectively. The nanocomposite showed excellent stability after five cycles of dye reduction.

Construction of novel potentiometric sensors modified with biogenically synthesized metal oxide nanoparticles for sensitive detection of the opioid agonist-antagonist nalbuphine hydrochloride in its injection.

Novel and sensitive potentiometric sensors were described for the assay of nalbuphine HCl (NBP) in authentic powder and injection samples. The developed sensors were modified with alumina nanoparticles (Al2O3NPs) and copper oxide nanoparticles (CuONPs). The nanoscale materials were synthesized using the extract of Salvia officinalis leaves in an environmentally friendly manner. The synthesized metal oxides were fully confirmed by various analytical techniques. Scanning electron microscope confirmed the morphology of nanosized materials with even distribution and particle size of 55.07 ± 4.15 and 59.48 ± 4.50 nm for Al2O3NPs and CuONPs, respectively. The modified sensors were prepared in three different steps. Nalbuphine hydrochloride was mixed with phosphomolybdic acid to prepare the sensor material nalbuphine phosphomolybdate (NBP-PM). It was then mixed with polyvinyl chloride in the presence of o-nitrophenyl ether and metal oxide nanoparticles to form the membrane matrix. Finally, a copper wire was coated with the sensing material. Excellent potentials of 1.0 × 10-8-1.0 × 10-2 and 1.0 × 10-9-1.0 × 10-2 mol L-1 were measured with lower assay limits of 4.8 × 10-9 and 5.0 × 10-10 mol L-1. The average detection % were 99.28 ± 0.58% and 99.52 ± 0.28% for NBP-PM-Al2O3NPs and NBP-PM-CuONPs, correspondingly. The suitability of the described sensors was investigated in terms of various validation criteria, and the modified sensors exposed excellent applicability and insurance for the quantification of nalbuphine hydrochloride in its bulk samples and injections compared with another standard sensor. It is obvious that the developed NBP-PM-Al2O3NPs and NBP-PM-CuONPs will serve as suitable sensors for the determination of NBP.

Effect of potential microplastics in sewage effluent on Nile Tilapia and photocatalytic remediation with zinc oxide nanoparticles.

The aim of the present study was a qualitative assessment of potential microplastics (MPs) in the sewage effluent collected from a local sewage treatment plant located in Riyadh City, Saudi Arabia. The composite samples of domestic sewage effluent were subjected to UV (ultraviolet) light-induced zinc oxide nanoparticles (ZnONPs) mediated photocatalysis. The first phase of the study included the synthesis of the ZnONPs with an extensive characterization. The synthesized nanoparticles were 220 nm in size with a characteristic spherical/hexagonal shape. These NPs were then used at three different concentrations (10 mM, 20 mM, and 30 mM) for the UV light-induced photocatalysis. A shift in the Raman spectra on photodegradation mirrored the surface changes of the functional groups shown by the FTIR spectra; presence of functional groups containing oxygen and C-C bonds associated with oxidation and chain scission. SEM micrographs showed photodegraded particles. Complementary elemental maps from the EDS analysis showed the presence of C, O, and Cl suggesting the potential presence of MPs. The O/C ratio was used to assess potential oxidation degree. In addition, an evaluation of the toxicological effects of the potential MPs in the sewage effluent on Nile tilapia (Oreochromis niloticus) exposed to the effluent at two concentrations (50% and 75%) elicited a marked response in the endpoints evaluated; EROD activity, MDA (malondialdehyde), 8-oxo-2'-deoxyguanosine levels in and AChE (acetylcholinesterase) activity in the brain. Thus, the key results provide new insights into the use of clean technologies to combat global MP pollution in aquatic ecosystems.

Impact of silver-doped alumina nanocomposite on water decontamination by remodeling of biogenic waste.

The main cause of various fatal diseases in humans and animals is environmental pollution. Ag-doped alumina nanocomposite was prepared using coffee husk extract with a large BET surface area of 126.58 m2 g-1 and investigated for its antibacterial potential against both bacterial strains Escherichia coli and Salmonella typhimurium, and observed as an effective sorbent for removing the water pollution dye indigo carmine (IGC). The lowest concentration of the nanocomposite and the maximum contact time required to achieve complete inhibition of bacteria present in the contaminated water, as well as the capacity of sorption of IGC, were investigated. The results showed that the minimum inhibitory concentration of the Ag-doped alumina nanocomposite was 12 µg mL-1 for both bacterial strains, with the highest inhibition occurring in E. coli. Moreover, the nanocomposite exhibited an experimental qt of 462.7 mg g-1 from 160 mg L-1 IGC solution at 50 °C and followed the Langmuir model. The thermodynamic results showed that the process was endothermic, spontaneous, and physisorptive. The nanocomposite was used to fully treat water samples contaminated with 10 mg L-1 concentrations of IGC. For six consecutive cycles, the reuse research showed an average efficiency of 95.72 ± 3.6%. Consequently, the synthesized Ag-doped alumina nanocomposite is suitable for treatments of contaminated water.

Biogenic sunflower oil-chitosan decorated fly ash nanocomposite film using white shrimp shell waste: Antibacterial and immunomodulatory potential.

A new sunflower oil-chitosan decorated fly ash (sunflower oil/FA-CSNPs) bionanocomposite film was synthesized using the extract of Litopenaeus vannamei (White shrimp) and evaluated as an antibacterial and immunomodulatory agent. Fly ash-chitosan nanoparticles were produced by using chitosan (CS) isolated from white shrimp extract, glacial acetic acid and sodium tripolyphosphate solution as cross-linkage. The ultrafine polymeric sunflower oil-CS film was fabricated by treating fly ash-chitosan nanoparticles with sunflower oil in glacial acetic acid under continuous stirring for 24 h. The nanostructure of the fabricated polymeric film was confirmed and characterized by different microscopic and spectroscopic approaches. The surface morphology of pre-synthesized bionanocomposite film was found to be homogenous, even and without cracks and pores. The crystallinity of formed bionanocomposite film was noticed at angles (2θ) at 12.65°, 15.21°, 19.04°, 23.26°, 34.82°, and 37.23° in the XRD spectrum. The fabricated film displayed excellent stability up to 380 °C. The formed sunflower oil/FA-CSNPs bionanocomposite film showed promising antibacterial towards Bacillus subtilis with highest zone of inhibition of 34 mm and Pseudomonas aeruginosa with zone of inhibition of 28 nm. The as-synthesized bionanocomposite film exhibited highest cell viability effect (98.95%), followed by FA-CSNPs (83.25%) at 200 µg mL-1 concentrations. The bionanocomposite film exerted notable immunomodulatory effect by promoting phagocytosis and enhancing the production of cytokines (NO, IL-6, IL-1ß, and TNF-α) in macrophage-derived RAW264.7 cell line.

Advanced polymeric metal/metal oxide bionanocomposite using seaweed Laurencia dendroidea extract for antiprotozoal, anticancer, and photocatalytic applications.

Background: Biosynthesized nanoparticles are gaining popularity due to their distinctive biological applications as well as bioactive secondary metabolites from natural products that contribute in green synthesis. Methodology: This study reports a facile, ecofriendly, reliable, and cost-effective synthesis of silver nanoparticles (AgNPs), copper oxide nanoparticles (CuONPs), and polymeric PVP-silver-copper oxide nanocomposite using ethanol extract of seaweed Laurencia dendroidea and were evaluated for antiprotozoal, anticancer and photocatalytic potential. The nanostructures of the AgNPs, CuONPs, and polymeric PVP-Ag-CuO nanocomposite were confirmed by different spectroscopic and microscopic procedures. Results: The UV-vis spectrum displayed distinct absorption peaks at 440, 350, and 470 nm for AgNPs, CuONPs, and polymeric Ag-CuO nanocomposite, respectively. The average particles size of the formed AgNPs, CuONPs, and Ag-CuO nanocomposite was 25, 28, and 30 nm, respectively with zeta potential values -31.7 ± 0.6 mV, -17.6 ± 4.2 mV, and -22.9 ± 4.45 mV. The microscopic investigation of biosynthesized nanomaterials revealed a spherical morphological shape with average crystallite sizes of 17.56 nm (AgNPs), 18.21 nm (CuONPs), and 25.46 nm (PVP-Ag-CuO nanocomposite). The antiprotozoal potential of green synthesized nanomaterials was examined against Leishmania amazonensis and Trypanosoma cruzi parasites. The polymeric PVP-Ag-CuO nanocomposite exerted the highest antiprotozoal effect with IC50 values of 17.32 ± 1.5 and 17.48 ± 4.2 µM, in contrast to AgNPs and CuONPs. The anticancer potential of AgNPs, CuONPs, and polymeric PVP-Ag-CuO nanocomposite against HepG2 cancer cell lines revealed that all the nanomaterials were effective and the highest anticancer potential was displayed by PVP-Ag-CuO nanocomposite with IC50 values 91.34 µg mL-1 at 200 µg mL-1 concentration. Additionally, PVP-Ag-CuO nanocomposite showed strong photocatalytic effect. Conclusion: Overall, this study suggested that the biogenic synthesized nanomaterials AgNPs, CuONPs, and polymeric PVP-Ag-CuO nanocomposite using ethanol extract of seaweed L. dendroidea possesses promising antiprotozoal anticancer and photocatalytic effect and could be further exploited for the development of antiprotozoal and anticancer therapeutics agents.

Olive Leaf Extracts for a Green Synthesis of Silver-Functionalized Multi-Walled Carbon Nanotubes.

Green biosynthesis, one of the most dependable and cost-effective methods for producing carbon nanotubes, was used to synthesize nonhazardous silver-functionalized multi-walled carbon nanotubes (SFMWCNTs) successfully. It has been shown that the water-soluble organic materials present in the olive oil plant play a vital role in converting silver ions into silver nanoparticles (Ag-NPs). Olive-leaf extracts contain medicinal properties and combining these extracts with Ag-NPs is often a viable option for enhancing drug delivery; thus, this possibility was employed for in vitro treating cancer cells as a proof of concept. In this study, the green technique for preparing SFMWCNTs composites using plant extracts was followed. This process yielded various compounds, the most important of which were Hydroxytyrosol, Tyrosol, and Oleuropein. Subsequently, a thin film was fabricated from the extract, resulting in a natural polymer. The obtained nanomaterials have an absorption peak of 419 nm in their UV-Vis. spectra. SEM and EDS were also used to investigate the SFMWCNT nanocomposites' morphology simultaneously. Moreover, the MTT assay was used to evaluate the ability of SFMWCNTs to suppress cancer cell viability on different cancer cell lines, MCF7 (human breast adenocarcinoma), HepG2 (human hepatocellular carcinoma), and SW620 (human colorectal cancer). Using varying doses of SFMWCNT resulted in the most significant cell viability inhibition, indicating the good sensitivity of SFMWCNTs for treating cancer cells. It was found that performing olive-leaf extraction at a low temperature in an ice bath leads to superior results, and the developed SFMWCNT nanocomposites could be potential treatment options for in vitro cancer cells.

Atropine-Phosphotungestate Polymeric-Based Metal Oxide Nanoparticles for Potentiometric Detection in Pharmaceutical Dosage Forms.

The new research presents highly conductive polymeric membranes with a large surface area to volume ratio of metal oxide nanoparticles that were used to determine atropine sulfate (AT) in commercial dosage forms. In sensing and biosensing applications, the nanomaterials zinc oxide (ZnONPs) and magnesium oxide (MgONPs) were employed as boosting potential electroactive materials. The electroactive atropine phosphotungstate (AT-PT) was created by combining atropine sulfate and phosphotungstic acid (PTA) and mixing it with polymeric polyvinyl chloride (PVC) with the plasticizer o-nitrophenyl octyl ether (o-NPOE). The modified sensors AT-PT-ZnONPs or AT-PT-MgONPs showed excellent selectivity and sensitivity for the measurements of atropine with a linear concentration range of 6.0 × 10-8 - 1.0 × 10-3 and 8.0 × 10-8 - 1.0 × 10-3 mol L-1 with regression equations of E(mV) = (56 ± 0.5) log [AT] - 294 and E(mV) = (54 ± 0.5) log [AT] - 422 for AT-PT-NPs or AT-PT-MgONPs sensors, respectively. The AT-PT coated wire sensor, on the other hand, showed a Nernstian response at 4.0 × 10-6 - 1.0 × 10-3 mol L-1 and a regression equation E(mV) = (52.1 ± 0.2) log [AT] + 198. The methodology-recommended guidelines were used to validate the suggested modified potentiometric systems against various criteria.

New Isolated Shrimp (Litopenaeus setiferus) Chitosan-Based Films Loaded with Fly Ash for Antibacterial Evaluation.

New three fabricated chitosan (CS) loaded with fly ash (FA) films were developed in this study. The shell waste of white shrimp was used as a precursor for the isolation of chitin and converted into chitosan by carrying out a deacetylation process. The formation of chitosan was conducted by various preparation steps deproteinization, demineralization, and deacetylation. The degree of deacetylation was found to be 95.2%. The obtained chitosan was used to prepare three different chitosan loaded-fly ash films. The prepared films contained various fly ash: chitosan ratios (2:1, FA-CSF1), (1:1, FA-CSF2), and (1:2, FA-CSF3). The obtained films were characterized using FTIR, XRD, and SEM. The micrograph images of the formed films showed spherical particles with an average size of 10 µm. The surface area, adsorption-desorption properties, thermal stability, and water/fat binding features of the fabricated chitosan films were studied. The results revealed that the prepared films displayed typical BET graphs with surface areas ranging from 2.436 m2 g-1 to 8.490 m2 g-1. The fabricated FA-CSF films also showed high thermal stability at temperatures up to 284.9 °C and excellent water/fat binding capacities. The antibacterial potential of the designed films was screened against E. coli (Gram-negative) and B. cereus (Gram-positive) bacterial strains. The tested solution of CS (1%) exhibited inhibition zones for E. coli and B. cereus as 18.51 mm and 14.81 mm, respectively, while in FA solution (1%), the inhibition zones were found to be 10.16 mm, and 13.57 mm, respectively. The results encourage and open up the new and promising areas of research for applying chitosan extracted from waste materials in biological applications.

Antimicrobial Activity of Green Silver Nanoparticles Synthesized by Different Extracts from the Leaves of Saudi Palm Tree (Phoenix Dactylifera L.).

The Arabian desert is rich in different species of medicinal plants, which approved variable antimicrobial activities. Phoenix dactylifera L. is one of the medical trees rich in phenolic acids and flavonoids. The current study aimed to assess the antibacterial and antifungal properties of the silver nanoparticles (AgNPs) green-synthesized by two preparations (ethanolic and water extracts) from palm leaves. The characteristics of the produced AgNPs were tested by UV-visible spectroscopy and Transmitted Electron Microscopy (TEM). The antifungal activity of Phoenix dactylifera L. was tested against different species of Candida. Moreover, its antibacterial activity was evaluated against two Gram-positive and two Gram-negative strains. The results showed that AgNPs had a spherical larger shape than the crude extracts. AgNPs, from both preparations, had significant antimicrobial effects. The water extract had slightly higher antimicrobial activity than the ethanolic extract, as it induced more inhibitory effects against all species. That suggests the possible use of palm leaf extracts against different pathogenic bacteria and fungi instead of chemical compounds, which had economic and health benefits.

Advanced Functionalized CeO2/Al2O3 Nanocomposite Sensor for Determination of Opioid Medication Tramadol Hydrochloride in Pharmaceutical Formulations.

BACKGROUND: The exceptional characteristics of cerium oxide (CeO2) and aluminum oxide (Al2O3) nanoscales have inspired significant attention to those nanocomposites as possible electroactive resources for applications of sensing and biosensing. METHODS: In this research, an innovative new factionalized CeO2/Al2O3 nanocomposite membrane sensor was presented to assess tramadol hydrochloride (TRD) in marketable products. RESULTS: Tramadol-phosphomolybdate (TRD-PM) was formed by mixing tramadol hydrochloride and phosphomolybdic acid (PMA) in the attendance of polymeric matrix and o-nitrophenyloctyl ether solvent mediator. With 1.0 × 10-10-1.0 × 10-2 mol L-1 as a range of linearity and EmV = (57.567 ± 0.2) log [TRD] + 676.29 as a regression equation, the functionalized sensor using TRD-PM-CeO2/Al2O3 nanocomposite showed great selectivity and sensitivity for the discriminating and measurement of TRD. Using the regression equation EmV = (52.143 ± 0.4) log [TRD] + 431.45, the unmodified coated wire sensor of TRD-PM, on the other hand, showed a Nernstian response between 1.0 × 10-6 and 1.0 × 10-2 mol L-1, Using the methodology's specified guidelines, the proposed improved potentiometric system was validated against several criteria. CONCLUSION: The suggested method is suitable for the determination of TRD in its products.

Unfunctionalized and Functionalized Multiwalled Carbon Nanotubes/Polyamide Nanocomposites as Selective-Layer Polysulfone Membranes.

Nowadays, reverse osmosis is the most widely utilized strategy in membrane technology due to its continuous improvement. Recent studies have highlighted the importance of the surface characteristics of support layers in thin-film membranes to improve their reverse osmosis performance. In this study, interfacial polymerization was used to generate the membranes by employing polyamide as a selective layer on top of the polysulfone supporting sheet. Different membranes, varying in terms of the concentrations of unfunctionalized and functionalized multiwalled carbon nanotubes (MWCNTs), as well as ethanol, have been fabricated. The efficiency of the membrane has been increased by increasing its permeability towards water with high salt rejection. Different characterization techniques were applied to examine all of the fabricated membranes. PA-EtOH 30% (v/v), as a selective layer on polysulfone sheets to enhance the membrane's salt rejection, was shown to be the most efficient of the suggested membranes, improving the membrane's salt rejection. The water permeability of the polyamide membrane with EtOH 30% (v/v) was 56.18 L/m2 h bar, which was more than twice the average permeability of the polyamide membrane (23.63 L/m2 h bar). The salt rejection was also improved (from 97.73% for NaCl to 99.29% and from 97.39% for MgSO4 to 99.62% in the same condition). The PA-MWCNTs 0.15% membrane, on the other hand, had a reduced surface roughness, higher hydrophobicity, and higher water contact angle readings, according to SEM. These characteristics led to the lowest salt rejection, resulting from the hydrophobic nature of MWCNTs.

Fabrication of novel buckypaper metal oxide nano-catalysis glycerol carbonate/MWCNTs membrane for efficient removal of heavy metals.

This study describes the fabrication of novel buckypaper membranes through the dispersion of multi-walled carbon nanotubes (MWCNTs) in the presence of surfactants metal oxide nano-catalysis Zinc oxide and magnesium oxide (ZnO and MgO) glycerol carbonate separately. Following vacuum filtration of the scattered solutions, self-supporting membranes known as buckypapers (BPs) were produced. The suggested membranes were employed for the efficient removal of heavy metals. The obtained data indicated that the incorporation of both glycerol carbonates prepared by two different nano metal oxides enhanced the permeability of MWCNTs membranes rejection efficiency. The characterization of the synthesized metal oxide nanoparticles, as well as the physicochemical and morphological properties of the membranes, were investigated. The rejection capabilities of membranes for the heavy metal ions were examined. Moreover, the suggested MWCNTs/ZnO nano-catalyst glycerol carbonate BP membrane displayed high rejection efficiency for heavy metals (Cd2+, Cu2+, Co2+, Ni2+, and Pb2+) than that prepared from the MgO nano-catalyst one.

Synthesis, Biological, and Molecular Docking Studies on 4,5,6,7-Tetrahydrobenzo[b]thiophene Derivatives and Their Nanoparticles Targeting Colorectal Cancer.

Initiation of colorectal carcinogenesis may be induced by chromosomal instability caused by oxidative stress or indirectly by bacterial infections. Moreover, proliferating tumor cells are characterized by reprogrammed glucose metabolism, which is associated with upregulation of PDK1 and LDHA enzymes. In the present study, some 4,5,6,7-tetrahydrobenzo[b]thiophene derivatives in addition to Fe3O4 and Fe3O4/SiO2 nanoparticles (NPs) supported with a new Schiff base were synthesized for biological evaluation as PDK1 and LDHA inhibitors as well as antibacterial, antioxidant, and cytotoxic agents on LoVo and HCT-116 cells of colorectal cancer (CRC). The results showed that compound 1b is the most active as PDK1 and LDHA inhibitor with IC50 values (µg/mL) of 57.10 and 64.10 compared to 25.75 and 15.60, which were produced by the standard inhibitors sodium dichloroacetate and sodium oxamate, respectively. NPs12a,b and compound 1b exhibited the strongest antioxidant properties with IC50 values (µg/mL) of 80.0, 95.0, and 110.0 µg/mL, respectively, compared to 54.0 µg/mL, which was produced by butylated hydroxy toluene. Moreover, NPs12a and carbamate derivative 3b exhibited significant cytotoxic activities with IC50 values (µg/mL) of 57.15 and 81.50 (LoVo cells) and 60.35 and 71.00 (HCT-116 cells). Thus, NPs12a and compound 3b would be considered as promising candidates suitable for further optimization to develop new chemopreventive and chemotherapeutic agents against these types of CRC cell lines. Besides, molecular docking in the colchicine binding site of the tubulin (TUB) domain revealed a good binding affinity of 3b to the protein; in addition, the absorption, distribution, metabolism, and excretion (ADME) analyses showed its desirable drug-likeness and oral bioavailability characteristics.

Greener Synthesis of Zinc Oxide Nanoparticles: Characterization and Multifaceted Applications.

Nanoparticles (NPs) have unique properties compared to their bulk counterparts, and they have potentials for various applications in many fields of life science. Green-synthesized NPs have garnered considerable interest due to their inherent features such as rapidity, eco-friendliness and cost-effectiveness. Zinc oxide nanoparticles (ZnO NPs) were synthesized using an aqueous extract of Kalanchoe blossfeldiana as a reducing agent. The resulting nanoparticles were characterized via X-ray diffraction (XRD), dynamic light scattering (DLS), UV-Vis spectroscopy, photoluminescence (PL), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDS). The antimicrobial potential of the synthesized ZnO NPs against bacterial and fungal strains was examined by the disk diffusion method, and they showed a promising antibacterial and antifungal potential. The catalytic activity of the synthesized ZnO NPs in reducing methylene blue (MB) and eosin was studied via UV-Vis spectroscopy. The decolorization percentages of the MB and Eosin Y dyes were 84% and 94%, respectively, which indicate an efficient degradation of the ZnO NPs. In addition, the cytotoxic activity of the ZnO NPs on the HeLa cell line was evaluated via in vitro assay. The MTT assay results demonstrate a potent cytotoxic effect of the ZnO NPs against the HeLa cancer cell line.

Drug Design of Inhibitors of Alzheimer's Disease (AD): POM and DFT Analyses of Cholinesterase Inhibitory Activity of ß-amino di-Carbonyl Derivatives.

BACKGROUND: Since deficit of acetylcholine has been evidenced in the Alzheimer's disease (AD) patients, cholinesterase inhibitors are currently the most specified drug category for the remediation of AD. METHOD: In the present study, 16 compounds (1-16) with dicarbonyl skeletons have been synthesized and tested for their inhibitory potential in vitro against AChE and BChE using ELISA microtiter plate assays at 100 µg/mL. Since metal accumulation is related to AD, the compounds were also tested for their metal-chelation capacity. RESULTS AND CONCLUSION: All the investigated dicarbonyl compounds exerted none or lower than 30% inhibition against both cholinesterases, whereas compounds 2, 8 and 11 showed 37, 42, 41% of inhibition towards BChE, being the most active. The highest metal-chelation capacity was observed with compound 8 (53.58 ± 2.06%). POM and DFT analyses are in good harmonization with experimental data.