RESUMO
The SLICK1 mutation in the prolactin receptor (PRLR) results in a short-hair coat and increased ability to regulate body temperature during heat stress. It is unclear whether the mutation affects capacity for sweating. The objective of this observational study was to evaluate whether the SLICK1 mutation in PRLR alters characteristics of skin related to sweat gland abundance or function. Skin biopsies from 31 Holstein heifers, including 14 wild-type (SL-/-) and 17 heterozygous slick (SL+/-), were subjected to histological analysis to determine the percent of the surface area of skin sections that are occupied by sweat glands. We detected no effect of genotype on this variable. Immunohistochemical analysis of the forkhead transcription factor A1 (FOXA1), a protein essential for sweating in mice, from 6 SL-/- and 6 SL+/- heifers indicated twice as much FOXA1 in sweat glandular epithelia of SL+/- heifers as in SL-/- heifers. Results from RNA sequencing of skin biopsies from 5 SL-/- and 7 SL+/- heifers revealed few genes that were differentially expressed and none that have been associated with sweat gland development or function. In conclusion, results do not support the idea that the SLICK1 mutation changes the abundance of sweat glands in skin, but do show that functional properties of sweat glands, as indicated by increased abundance of immunoreactive FOXA1, are modified by inheritance of the mutation in PRLR.
Assuntos
Receptores da Prolactina , Glândulas Sudoríparas , Animais , Bovinos , Feminino , Camundongos , Fatores de Transcrição Forkhead/genética , Expressão Gênica , MutaçãoRESUMO
A spectrofluorometric method of protein analysis using fluorescamine was shown to be a meaningful method of evaluating denture cleanser efficacy. This assay was useful in the presence of most currently used denture cleanser ingredients, materials which cause other protein assay methods to fail. The determination of dental plaque protein was shown to be an adequate measure of dental plaque dry weight.