RESUMO
BACKGROUND: The process of tissue injury in coronary artery disease (CAD) has been associated with activation of the complement system, partly due to the action of mannose-binding lectin (MBL) and C3, which are expressed in atherosclerotic lesions. OBJECTIVE: The aim of this study was to evaluate the serum levels of MBL and C3 in patients with CAD and to compare them with healthy controls. Additionally, we aim to assess the correlation between MBL and C3 levels and cardiometabolic parameters. METHODS: MBL and C3 serum concentration were determined by ELISA and immunoturbidimetry, respectively, in up to 119 patients undergoing coronary angiography for CAD evaluation, comprising 48 individuals diagnosed with acute myocardial infarction (MI) and 71 without MI. A total of 93 paired healthy controls were included in the study. RESULTS: Individuals with CAD had MBL serum concentration higher than controls (p = .002), regardless of the presence of MI (p = .006). In addition, high concentration of MBL (>2000 ng/mL) was more frequent in patients with CAD (p = .007; OR = 2.6; 95% CI = 1.3-5.1). C3 levels were not significantly associated with any of the patient groups but were positively correlated with cardiometabolic parameters such as body mass index (BMI) and triglycerides levels. CONCLUSIONS: Higher concentrations of MBL were found to be associated with CAD, whereas C3 levels were found to be associated with cardiovascular risk factors.
RESUMO
Neste trabalho investigou-se a prevalencia das infeccoes pelos virus HIV, HTLV e pelo Treponema pallidum em uma populacao indigena Kaingang (150 individuos) e mestica (64 individuos) da reserva de Mangueirinha, no Estado do Parana, Brasil. Osresultados demonstraram ausencia de positividade para HIV 1/2, HTLV 1/2 e Sifilis nos individuos investigados.
This study investigated the prevalence of HIV, HTLV and Treponema pallidum infections in 150 Kaingang Indians andin 64 individuals of mixed race Kaingang with non Indians from the Mangueirinha reservation in the state of Paraná, Brazil. The results showed that none of the individuals was positive for HIV 1/2, HTLV 1/2 and Syphilis.
Assuntos
Humanos , Masculino , Feminino , Lactente , Pré-Escolar , Criança , Adolescente , Adulto , Pessoa de Meia-Idade , Idoso de 80 Anos ou mais , Infecções por Deltaretrovirus , HIV , Índio , Estudos Soroepidemiológicos , SífilisRESUMO
RACIONAL: A doença celíaca representa, na atualidade, a doença intestinal mais comum em populações caucasóides e apresenta prevalência que varia de 8 por cento a 18 por cento nos familiares dos pacientes. A pesquisa dos anticorpos anti-endomísio (EmA-IgA) e antitransglutaminase tecidual (anti-tTG-IgA) constitui importante recurso não-invasivo e sensível de triagem e diagnóstico da doença celíaca em grupos de risco e populações. OBJETIVO: Avaliar a prevalência do EmA e anti-tTG em um grupo de familiares de celíacos e verificar o grau de concordância entre os dois métodos. MÉTODOS: Foram estudados 177 familiares (76(feminino); 101(masculino); 2-79 anos) e 93 indivíduos voluntßrios e sadios (34 (feminino); 59 (masculino); 2-71 anos) como grupo controle. O EmA foi detectado por imunofluorescência indireta (substrato: cordão umbilical humano) e o anti-tTG pelo método de ELISA (kit comercial). RESULTADOS: A positividade total de anticorpos nos familiares em estudo foi de 21 por cento (37/177), mostrando significativa diferença em relação aos controles (0 por cento; 0/93). Doze por cento (21/177) dos familiares foram positivos para o EmA e 13,56 por cento (24/177) para o anti-tTG, sendo 4,52 por cento (8/177) positivos concomitantemente para os dois anticorpos. A concordância de resultados entre os dois métodos foi de 83,6 por cento (148/177) e a discordância de 16,4 por cento (29/177), caracterizando uma correlação positiva significante (r= 0.435) entre ambos. Dentre os concordantes, 79,1 por cento (140/177) eram negativos para o anti-tTG e EmA, e 4,52 por cento (8/177) positivos para ambos. Nos casos discordantes, 7,34 por cento (13/177) apresentaram EmA positivo e anti-tTG negativo e 9,04 por cento (16/177) eram anti-tTG positivo e EmA negativo. CONCLUSÃO: Embora a alta positividade obtida para o EmA e anti-tTG destaque a importância da triagem sorológica em familiares de pacientes com doença celíaca, as discordâncias detectadas no estudo...
BACKGROUND: Celiac disease is the most common intestinal disorder of caucasian populations and presents a prevalence of 8 percent to 18 percent between the relatives of patients. The anti-endomysial (IgA-EmA) and anti-tissue transglutaminase antibodies (IgA-tTG) have represented an important non invasive and sensitivity method of screening and diagnosis of celiac disease in risk groups and populations. AIM: To investigate the prevalence of IgA-EmA and IgA-tTG antibodies in relatives of celiac patients and verify the degree of concordance between them. METHODS: One hundred and seventy seven relatives of celiac patients (76(feminino); 101(masculino); 2-79 years) and 93 healthy individuals were evaluated (34(feminino); 59(masculino); 2-71 years). IgA-EmA were detected by indirect immunofluorescence, with human umbilical cord as substrate, while anti-IgA-tTG titers were measured by enzyme-linked immunosorbent assay (ELISA), using commercial kit. RESULTS: Total positivity to antibodies in relatives of celiac patients was of 21 percent (37/177), and showed significant difference compared to control group (0 percent; 0/93). Twelve percent (21/177) of celiac disease relatives were positive to IgA-EmA, 13.56 percent (24/177) to IgA-tTG, and 4.52 percent (8/177) to both assays simultaneously. The concordance between both methods was 83.6 percent (148/177) and the discordance was 16.4 percent (29/177), with a positive and significant correlation (r = 0.435). Among the concordant results, 79.1 percent (140/177) were negative and 4.52 percent (8/177) were positive to both antibodies. Among the discordant results, 7.34 percent (13/177) were positive to IgA-EmA and negative to IgA-tTG, while 9.04 percent (16/177) were negative to IgA- EmA and positive to IgA-tTG. CONCLUSION: Although the high positivity to IgA-EmA and IgA-tTG emphasizes the importance of the serological screening in relatives of celiac patients, the discordances detected in this study...