A multidisciplinary approach to forensic biological profiling on a single tooth and nail sample.

INTRODUCTION: Analysis of a single tooth and nail can provide valuable forensic information, including year of birth, year of death, age, sex, DNA-profile, geographic residence during childhood and at time of death and drug exposure. The aim is to minimize the amount of used bodily material and to validate the applicability of a multidisciplinary sampling protocol. METHODS: A nail of the big toe, a tooth and blood of seven deceased individuals were collected postmortem. Collected materials were sampled and segmented in accordance with the multidisciplinary sampling protocol. DNA analysis was conducted on the pulp of the tooth, isotope analysis (Sr, Pb, O and C) on the enamel and 14C-, toxicological and tooth cementum annulation analysis on root segments. DNA-, isotope (Sr, Pb, O and C) -, toxicological-, and 14C -analysis were conducted on toenail segments. The acquired DNA profiles were compared with profiles acquired from blood. RESULTS: Material from seven deceased persons was analysed. 45 out of 56 analyses on dental samples were successful, constituting a success rate of 80%. Additionally, 27 out of 35 analyses were successful on nail samples, yielding a success rate of 77%. DNA-, toxicological and 14C- analyses performed better in nail than in tooth. Isotope analyses performed better in tooth than in nail. A profile with personal characteristics was constructed and matched for 62% of parameters with collected medical information. CONCLUSION: The performed sampling protocol for simultaneous multidisciplinary forensic analysis on a single tooth and nail sample provided applicable results and valuable information.

Enhancing the contemporary human and water isotope reference database for the Netherlands: New insights from Sr-O-C-N-H isotope data.

The determination of an individual's geographic origin is an essential aspect of forensic investigations. When primary identifiers cannot be used to make a positive identification, isotope analysis can be utilized to provide new leads. Modern reference data are essential for accurate interpretation of human isotopic data in terms of diet and origin. This article presents Sr-O-C-N-H isotope data of modern individuals (hair, dental enamel, and dentine collagen) and drinking water from the Netherlands. The δ15N values of human hair fall within the range of values observed worldwide and cannot be utilized to differentiate from other countries. Distinct disparities in the hair δ13C are evident between European countries and other regions, making it possible to exclude the Netherlands as a region of origin. Comparing Dutch dental isotope data to those of other nations has proven difficult due to the limited availability of reference data. The same limitation applies to tap water δ2H data.

Isotopic analysis of formula milk reveals potential challenges in geolocating bottle-fed babies.

In forensic investigations involving the identification of unknown deceased individuals, isotope analysis can provide valuable provenance information. This is especially pertinent when primary identifiers (i.e., DNA, dactyloscopy, etc.) fail to yield matches. The isotopic composition of human tissues is linked to that of the food consumed, potentially allowing the identification of regions of origin. However, the isotopic composition of deceased newborns and infants fed with milk formula may be influenced by that of the prepared milk. The findings contribute towards the possibility to isotopically identify bottle-fed infants. More importantly, the data convincingly show that the Sr isotope composition of the prepared milk is determined by that of the formula and not the (local) tap water, thereby limiting the potential of Sr isotope analysis for determining the geological or geographical origin in formula-fed babies in medico-legal cases.

Sr-Pb isotope differences in pre- and post-burial human bone, teeth, and hair keratin: implications for isotope forensics.

The isotopic signatures of human tissues can provide valuable information on geographic origin for medicolegal investigations involving unidentified persons. It is important to understand the impact of diagenetic processes on isotopic signatures, as alterations could result in incorrect estimation of geographic origin. This study examines alterations in isotope signatures of different tissues of five human body donors studied throughout decomposition at the Forensic Anthropology Research Facility (FARF), San Marcos, TX. Two body donors were buried, two were placed in open pits, and one was first allowed to naturally mummify and then buried. Remains were recovered after a period of 7-34 months. The preplacement and post-recovery Sr-Pb isotope data of scalp hair, bone (iliac and tibia), and tooth enamel and dentine were compared. The hair samples record significant shifts in Sr-Pb isotope compositions, with hair keratin Pb isotope composition shifting towards the Pb signature of local soil samples. Hair keratin Sr isotope compositions were altered by the burial environment and possibly also by the lab sample cleaning method. The spongy iliac bone samples show inconsistencies in the recoverability of the preplacement Sr-Pb isotope signatures. The post-placement signatures of the buried donors show slight elevation over preplacement signatures. The post-placement signatures of donors placed in open pits are significantly elevated. The tibia and dental samples record the most consistent isotopic data with the least alteration. These more densely mineralised elements show good recoverability of the preplacement isotope signatures in burials and open pits and are thus deemed better targets for forensic investigative purposes.

Multi-isotopes in human hair: A tool to initiate cross-border collaboration in international cold-cases.

Unidentified human remains have historically been investigated nationally by law enforcement authorities. However, this approach is outdated in a globalized world with rapid transportation means, where humans easily move long distances across borders. Cross-border cooperation in solving cold-cases is rare due to political, administrative or technical challenges. It is fundamental to develop new tools to provide rapid and cost-effective leads for international cooperation. In this work, we demonstrate that isotopic measurements are effective screening tools to help identify cold-cases with potential international ramifications. We first complete existing databases of hydrogen and sulfur isotopes in human hair from residents across North America by compiling or analyzing hair from Canada, the United States (US) and Mexico. Using these databases, we develop maps predicting isotope variations in human hair across North America. We demonstrate that both δ2H and δ34S values of human hair are highly predictable and display strong spatial patterns. Multi-isotope analysis combined with dual δ2H and δ34S geographic probability maps provide evidence for international travel in two case studies. In the first, we demonstrate that multi-isotope analysis in bulk hair of deceased border crossers found in the US, close to the Mexico-US border, help trace their last place of residence or travel back to specific regions of Mexico. These findings were validated by the subsequent identification of these individuals through the Pima County Office of the Medical Examiner in Tucson, Arizona. In the second case study, we demonstrate that sequential multi-isotope analysis along the hair strands of an unidentified individual found in Canada provides detailed insights into the international mobility of this individual during the last year of life. In both cases, isotope data provide strong leads towards international travel.

Comparison of strontium isotope ratios in Mexican human hair and tap water as provenance indicators.

Deceased undocumented border crossers are some of the most difficult individuals to identify due to the inability to narrow down the region of origin and therefore to obtain family reference samples for DNA comparison. The isotopic compositions of various body tissues have been demonstrated to be useful biomarkers for tracking locations and movements to aid in the identification of human remains. This study closes the large spatial gap of available 87Sr/86Sr ratios from North America in tap water and presents the first 87Sr/86Sr human tissue-based ratios from Mexico. The 101 hair samples from 32 locations in Mexico range in 87Sr/86Sr ratios from 0.70424 to 0.71613 (ΔSrmax-min=0.01189). Furthermore, 151 tap water samples from 51 locations range between 0.70404 to 0.71385 (ΔSrmax-min=0.00981). Overall, small variations in the hair and tap water samples collected from individual locations were recorded (ΔSrmax-min=0.00041 and 0.00034 respectively). Despite the fact that Mexico is one of the largest bottled water consumers in the world, the 87Sr/86Sr ratios of human hair and tap water correlated strongly (R2=0.87 for location averages and R2=0.80 when using individual data points). These data represent a valuable resource for identifying the provenance of human remains.

Spatial patterns in 87Sr/86Sr ratios in modern human dental enamel and tap water from the Netherlands: Implications for forensic provenancing.

The analysis of strontium isotope ratios in human dental enamel has become important in the fields of archaeological and forensic science for determining provenance and hence mobility. The prerequisite for the approach relies on a correlation between dietary Sr intake and the underlying local geology. This premise is brought into question for anthropological forensic investigations by the increasing globalisation of food supply, the establishment of nation-wide or international supermarket chains, and increasing urbanisation. To better understand the processes that cause spatial variation of Sr isotope ratios in the modern environment, this study determines the range of 87Sr/86Sr ratios in the modern Dutch environment based on 296 modern human dental enamel and tap water samples. Tap water 87Sr/86Sr from the Netherlands range from 0.70837 to 0.71278 (ΔSrmax-min = 0.0044) and modern human enamel from 0.70847 to 0.70995 (ΔSrmax-min = 0.0015). The 87Sr/86Sr ratios of tap water are predominantly determined by the underlying bedrock geology at the sampling point. In contrast, the human enamel data record an insignificant, weak correlation with water supply or local geology. Hence, the main principle behind the application of 87Sr/86Sr as a proxy for mobility appears invalid in the modern globalised Dutch context. The range of 87Sr/86Sr in modern Dutch humans that can be used for anthropological forensic investigations is between 0.7085 and 0.7100 (n = 153), with 98.0% of individuals between 0.7088 and 0.7099.

Spatial Distributions of Oxygen Stable Isotope Ratios in Tap Water From Mexico for Region of Origin Predictions of Unidentified Border Crossers.

One of the many difficulties associated with identifying undocumented border crossers stems from an inability to narrow down the search area for the region of origin and family members to obtain family reference samples for DNA comparison. While the geography of regions of origins is wide, the biological profiles of the undocumented border crossers often show strong similarities, young and male. The isotopic composition of human bones, teeth, and hair has been demonstrated to be useful biomarkers for tracing locations and movements of individuals and for aiding in the identification of human remains. Data obtained from human remains can be compared to and aligned with various reference sources, such as soils and bedrock, archaeological remains, or cultural data. Here, the spatial deficiency in isotopic reference data for Mexico, specifically for oxygen (δ18 O) isotopes in tap water, is being addressed through the collection and analysis of over 150 water samples and explored with tooth enamel isotopic values from recently identified Mexican nationals. The isoscape was developed using a Spine with Barriers interpolation method and shows sufficient detail to narrow down the regions where specific isotopic values are represented. The individuals were plotted on the developed isoscape using conditional correlations. The methodology was successful in assigning the correct regions of origin for the two individuals, which shows the tremendous potential of the developed isoscape. Nonetheless, there is more research needed to further improve upon this geolocation method, including analysis of multiple isotopes in different tissues, and the development of new isotopic methods.